LOCUS       62056_20015        6573 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector pSMXL8-LUC, complete sequence.
ACCESSION   MN728548
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..6573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..2000
                     /label=promoter of AtSMXL8
                     /note="promoter of AtSMXL8"
                     /regulatory_class="promoter"
     CDS             2008..3657
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      3678..3930
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3939..3955)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4429..4533
                     /label=AmpR promoter
     CDS             4534..5391
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5565..6153
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6441..6462
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6477..6507
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6515..6531
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6539..6555
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaaaaattat tccaatcagt gagagatctt caaaggaaag attctcattc gcgccaatat
       61 ttttgctggg aaaaggtaaa actttttaga ttattctata aattattttg ctgatgttcc
      121 cttacgaaaa aaacagtatt cagtaccaaa atgtctggca catataatcg tatagacgat
      181 aagtagttgt cgaaatttta tagggcaatg gctcaagcaa gtgaaacatt gacacctagc
      241 ccattcacat ctatgtcact atatatgtgg gttcacaaag gtgacgtttt ccttatgtga
      301 atcatgtaaa tgctataaag ttttgtagta catgagcgat tatatattat tacgaaactc
      361 ctgaaatata tgatatataa tcagatgggt tgaggtagtg acaatagttt gtaagtgcac
      421 caaaatacgt tttgagctat taagtccgaa gtttaggtcc ttttaagtta tcgaaggtat
      481 gcttaaaaaa atttactacc ttttagtttt tttttgcatg atcgttattc gagaaacatg
      541 ctgacgcaca gattgatcag acgctgtggt aatccaagtg atagcaacaa cacaaaatca
      601 tgtggagaat gatgatgatg atgcacatat ggatttgcat aaattttgta atctccgaaa
      661 tattgtggag aatgaaggag gagaaacata acattttaaa aatggataaa gaaaaagaaa
      721 gtgatggaaa aataagtatg tggtagaaaa atagggttcc ttcttctcat ttgtctcttt
      781 caagctttat gatgcaaagc gacccttctt tttcttccct ttattttctt ttattgttat
      841 cgttagtgtc actttctttt tattaatttc ataaagtcgt atgatcgtat ccattgtttt
      901 tattttgctc cgacaggacc gacactgatc tttctatatc tttcggtaat taatgttata
      961 ggattccttt ttcagatcac atcatttgta ttttatgtta ttttcgttta acataataca
     1021 ctaataaaat gacttcataa aagatatggt ttaggttggg gaataaaaga tatggtcgag
     1081 ccatacttct aatccttaat ttaggttgat agaacatcta ccgtcactaa caagtaatga
     1141 agtcagatta aaacttgatc gtgacaaaaa ttgtcccata tattttaaat gaacatgata
     1201 acatattaat tgaaaaatgt tttagtgact tagttgtatt gaatagcaaa ggcaagtaag
     1261 tagttatata caatgacttt cctatactcg agaagaccgt atccaagtaa atcaagaaga
     1321 tcgatatata tctagccgga tctttggatt gtcaagtgtc aacaatctta attagatcaa
     1381 aacagtctgt tttaaaacat atgccaagtt gtcaaacaga aaccaagctt ttcatcactc
     1441 aaaggagaat cacaaattct gcatggcgtg ggggatagat atatatcatc gggagccact
     1501 gagtccaaga aattgaatct attgacttcc tattgctaat ctatttcaaa ataaccacgt
     1561 cagagagata cgagtacatg taatatcaat gagatatttt cttaggagta atttgaagat
     1621 atttaatttg cggttgtaat aatccctttt gaaaataatt tgacttgtac acacggcaac
     1681 aaaagaatga tctaaaggca agtaacaaga atctgagtat ccaccacctc ttcctgttcc
     1741 tgttcttgtt cctgttcgat tccaaactct cactttctct gcccccctcg tgtctggctc
     1801 ccaccttttg tgtccactca accaacaaca acagtgtatc tcttctcctc cgttaaataa
     1861 ctttatattt ccccgttaca tttccttatt gtaacaacca aaaaataatt cacctgagac
     1921 tcacattctt taaggttttc gcatttaata tttcactttc ttgaaatctc ctatattttt
     1981 gttatatatg gtcgtcggcg gtcgaccatg gaagacgcca aaaacataaa gaaaggcccg
     2041 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
     2101 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
     2161 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     2221 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     2281 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     2341 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
     2401 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
     2461 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
     2521 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
     2581 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
     2641 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc
     2701 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     2761 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
     2821 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta
     2881 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     2941 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc
     3001 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt
     3061 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     3121 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
     3181 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     3241 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     3301 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
     3361 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
     3421 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     3481 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     3541 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     3601 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa
     3661 ttctagcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     3721 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     3781 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     3841 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     3901 cgcgcgcggt gtcatctatg ttactagatc gggaattcac tggccgtcgt tttacaacgt
     3961 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4021 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4081 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4141 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4201 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4261 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4321 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4381 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4441 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4501 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4561 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4621 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4681 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4741 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4801 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4861 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4921 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4981 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5041 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5101 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5161 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5221 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5281 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5341 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5401 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5461 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5521 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5581 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5641 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5701 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5761 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5821 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5881 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5941 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6001 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6061 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6121 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6181 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6241 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6301 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6361 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6421 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     6481 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6541 ggaaacagct atgaccatga ttacgccaag ctt
//
