LOCUS       62056_20010        6791 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector pSMXL7-WT-LUC, complete sequence.
ACCESSION   MN728549
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..6791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    633..655
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     promoter        717..1062
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1073..2005
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
     polyA_signal    2065..2241
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     2405..2421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2428..2446
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2472..2488
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2497..2980
                     /label=part of SMXL7 promoter
                     /note="part of SMXL7 promoter"
                     /regulatory_class="promoter"
     CDS             2982..4628
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    4874..4898
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(4989..5577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5751..6563)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc
      541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct
      601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta
      661 tcgtacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag
      721 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt
      781 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat
      841 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca
      901 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
      961 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     1021 ccttcctcta tataaggaag ttcatttcat ttggagagga cagcccacca ccatgacttc
     1081 gaaagtttat gatccagaac aaaggaaacg gatgataact ggtccgcagt ggtgggccag
     1141 atgtaaacaa atgaatgttc ttgattcatt tattaattat tatgattcag aaaaacatgc
     1201 agaaaatgct gttatttttt tacatggtaa cgcggcctct tcttatttat ggcgacatgt
     1261 tgtgccacat attgagccag tagcgcggtg tattatacca gaccttattg gtatgggcaa
     1321 atcaggcaaa tctggtaatg gttcttatag gttacttgat cattacaaat atcttactgc
     1381 atggtttgaa cttcttaatt taccaaagaa gatcattttt gtcggccatg attggggtgc
     1441 ttgtttggca tttcattata gctatgagca tcaagataag atcaaagcaa tagttcacgc
     1501 tgaaagtgta gtagatgtga ttgaatcatg ggatgaatgg cctgatattg aagaagatat
     1561 tgcgttgatc aaatctgaag aaggagaaaa aatggttttg gagaataact tcttcgtgga
     1621 aaccatgttg ccatcaaaaa tcatgagaaa gttagaacca gaagaatttg cagcatatct
     1681 tgaaccattc aaagagaaag gtgaagttcg tcgtccaaca ttatcatggc ctcgtgaaat
     1741 cccgttagta aaaggtggta aacctgacgt tgtacaaatt gttaggaatt ataatgctta
     1801 tctacgtgca agtgatgatt taccaaaaat gtttattgaa tcggacccag gattcttttc
     1861 caatgctatt gttgaaggtg ccaagaagtt tcctaatact gaatttgtca aagtaaaagg
     1921 tcttcatttt tcgcaagaag atgcacctga tgaaatggga aaatatatca aatcgttcgt
     1981 tgagcgagtt ctcaaaaatg aacaataatt ctagccggta cgctgaaatc accagtctct
     2041 ctctacaaat ctatctctct ctattttctc cataaataat gtgtgagtag tttcccgata
     2101 agggaaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
     2161 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     2221 tccagtacta aaatccagat cgataacatt aacgtttaca atttccattc gccattcagg
     2281 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     2341 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     2401 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attgggtacc
     2461 gggccccccc tcgaggtcga cggtatcgat aagcttttaa ttgaaaattt gaagagttta
     2521 agcgaatgga agcaaaggaa gatctttgag ttgggccata gagaatctat gctcaccaca
     2581 tgtgcctttc tttgcctttc ctccttcaat atacacgagt atttggggca acacataaca
     2641 acacataaca acaacaacaa caaaaattac acaaacaaaa acatttcata ataataacaa
     2701 ataatcattc aaaatataaa tataaaatat attagtgggt tccatcgcca atagaccacc
     2761 aaacagcgac tcttctttct tacatatcta tcttcacaag cttctgagga ttgaaaccaa
     2821 agaaagaaac gtacaaacat aaaacataaa aactatatat tataaattaa aaacacttct
     2881 ttttcataaa agcgactaca cggttttcat ttctttgtcg taaaaatccc atttccagct
     2941 ttaaaaattt cacgctttgt ttttgtttag gtctcatgaa agaagacgcc aaaaacataa
     3001 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
     3061 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     3121 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
     3181 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     3241 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     3301 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     3361 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     3421 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     3481 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     3541 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     3601 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     3661 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     3721 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     3781 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     3841 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     3901 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     3961 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     4021 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     4081 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     4141 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     4201 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     4261 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     4321 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
     4381 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     4441 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     4501 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     4561 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     4621 tcgccgtgta attctagaga attcgctgaa atcaccagtc tctctctaca aatctatctc
     4681 tctctatttt ctccataaat aatgtgtgag tagtttcccg ataagggaaa ttagggttct
     4741 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     4801 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca
     4861 gatccactag ccttgacagg atatattggc gggtaaacta agtcgctgta tgtgtttgtt
     4921 tgagatctca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5401 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5461 ccagttacct tcggaagaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5641 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5701 agttttaaat caatctaaag tatatatgtg taacattggt ctagtgatta gaaaaactca
     5761 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     5821 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     5881 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     5941 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     6001 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     6061 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     6121 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     6181 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     6241 tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     6301 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     6361 tcatctgtaa caacattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     6421 tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc
     6481 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac
     6541 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
     6601 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
     6661 acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat
     6721 cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc
     6781 acctacaaca a
//
