LOCUS       62056_20000        7573 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector pSMXL7-LUC, complete sequence.
ACCESSION   MN728547
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..3000
                     /label=promoter of AtSMXL7
                     /note="promoter of AtSMXL7"
                     /regulatory_class="promoter"
     CDS             3008..4657
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      4678..4930
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4939..4955)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5429..5533
                     /label=AmpR promoter
     CDS             5534..6391
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6565..7153
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7441..7462
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7477..7507
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7515..7531
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7539..7555
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctattttatt tatgtgacag tttggatttg ttgagagtat gattctaatg aatttgataa
       61 ttagtatcct cttactgcaa tagtaagagg tatccttttt tttgtcgcaa aggtagatta
      121 cttgttatat tagatattag cacaattaca actgacttgc agaaagtcag accaaaaaat
      181 gagatcaagg ggcacaagaa acacccacta aaaggtcaac cctgagaatc caaaaattaa
      241 cagggcaaca aaagaataag cctcccaaaa agcaaggagc ctaaaagcaa aaagacttca
      301 gtccacagag gttagaactc tctcatccct aaagacacca agaagccaag caggaaaact
      361 tacggccacg tacgattgaa gcctacactc cttagtaaca ctttgggcaa tgagattagc
      421 acctctattc gaagacaccg attcaagcac cactttccaa actgcaatgc catttaaagc
      481 taacaagatc tccgaagact gaaacctaaa agaaggccaa gcttgcggcc tgtttaacac
      541 cccaacaagc accgcagctt ccacaccaaa aaccacccta ttcacatgat gactaaccat
      601 actctcaaca gcccaaacaa tgcttagaaa ttgagcctcc aaaaaggagg aaatattgga
      661 gaaggctctt ctactgtgca tcaagacatt gcctctgtca ttcctcaaca cccaagcaac
      721 ccctgcaagt ctagtaagag gtatcctatt ttgtacaaaa aaactattat gattttgaat
      781 taccaatcga attaaattta aatcatacaa gcgatcaaat taatatttga atcaaaattt
      841 actaatttat aaattagctg aaaccaaaaa tataattaaa ttgaaaagca gccggtagtc
      901 caatgatcaa tgtttgtttg tctttaattg gcctgaaaat aataaaacta ttttatggta
      961 ataatgattg gtcattgagt aaatttcctt ttcctaaagg ggaaatacaa aaggagacct
     1021 ttgtggcgga gaatataaaa tgtggaagat aaaagtaaaa caaaaaagga agtttttttt
     1081 tttcttcttc atgattatta atgaaagact ttttgaatga caatgacacc aaaaacaaaa
     1141 aagcatataa atggtggtgg tggaatttat aaaggagttg gggcctaatc acttactcat
     1201 gcttttattc tttaccatat cacatccatc taatctctta aaagtaaaat tatatcactg
     1261 tctaccactg aatatatgct tagttataat taagttgact tgttgttaat catttctatt
     1321 atcttcaagt tatacgtctc aaaatttaaa taattgaatt ttcacaaaaa aaaaatctat
     1381 ttgtaaagta tcagtcagta tgcaaatcaa ttcattttaa ctttttctga acaaaaatat
     1441 ttctatcatc atcattgtgt ttaatttttt tccaaaacac tcgattacaa tctttgtata
     1501 gcttttaaac tcttaacata gccatatgta aatagatatc cgacatttta aaacccgtaa
     1561 gtacacagat tatctctctg aactgattct gtttagaaac tatgcatctt ttactagtta
     1621 acttgttgtg aattctagca ctatgcatct ttattggctt aatacagtgc aatggccaat
     1681 atatattttt cttttgtaac atagtgtatc ttagttttat atttcgttga atgaccttct
     1741 agatgattgt tacactgatt catctccatt ctcagcgtct tgtgataaat cttcaattta
     1801 taatgtccaa catttaacca tctataaaag aactaaacac atcgaaatag acttctagct
     1861 atatatgtga tttagaaaaa gattcattgt ttatgttcca aaatattcat atcatattgt
     1921 taatatacga acaaagtctt tgacatttcc tcttatagat catctcttat ccaagatttc
     1981 ttcgtaatat actaaaagaa gtaatcatca ttctagttta ttatgtcgcc aatgttttca
     2041 aacttggata attaatctgt ttgtttggta ccgtgaaaca aattttggtt tatttatcaa
     2101 attacagtaa aattcttaat attttttttt ccaacaatca atataggaat aaagctaaca
     2161 gaagttggtt gaacaaaact aacaaaagct caacacaatt aaaatgaatt acttggtctg
     2221 aatcgaacta ttaccttttg gaatatagtt agttaatgtt cacaatacga atacaagttg
     2281 gaaaaaacgg aaagaagaag atttggcata gatactcact cactcataca cttagatatt
     2341 tttctgtcta gctactttag ataagaataa gagaagagag aaaatcaaaa acaataaaaa
     2401 gaagaataat tgtgaaaaac aatgtggatt gtcaaatcca aacttaattg aaaatttgaa
     2461 gagtttaagc gaatggaagc aaaggaagat ctttgagttg ggccatagag aatctatgct
     2521 caccacatgt gcctttcttt gcctttcctc cttcaatata cacgagtatt tggggcaaca
     2581 cataacaaca cataacaaca acaacaacaa aaattacaca aacaaaaaca tttcataata
     2641 ataacaaata atcattcaaa atataaatat aaaatatatt agtgggttcc atcgccaata
     2701 gaccaccaaa cagcgactct tctttcttac atatctatct tcacaagctt ctgaggattg
     2761 aaaccaaaga aagaaacgta caaacataaa acataaaaac tatatattat aaattaaaaa
     2821 cacttctttt tcataaaagc gactacacgg ttttcatttc tttgtcgtaa aaatcccatt
     2881 tccagcttta aaaatttcac gctttgtttt tgtttatagt ttcttgtatg aattttattg
     2941 attggtgttg tggtttggtt tacttttaat ttcgaatttt ttataactaa accggcgacg
     3001 gtcgaccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga
     3061 agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg
     3121 aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga
     3181 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat
     3241 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat
     3301 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat
     3361 gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa
     3421 cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta
     3481 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga
     3541 atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc
     3601 ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag
     3661 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt
     3721 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg
     3781 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca
     3841 ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag
     3901 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc
     3961 cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca
     4021 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa
     4081 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac
     4141 cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat
     4201 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct
     4261 acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct
     4321 gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt
     4381 gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg
     4441 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat
     4501 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt
     4561 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat
     4621 cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ttctagcgaa tttccccgat
     4681 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     4741 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     4801 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     4861 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     4921 ttactagatc gggaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4981 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5041 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     5101 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     5161 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5221 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5281 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5341 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5401 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5461 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5521 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5581 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5641 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5701 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5761 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5821 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5881 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5941 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6001 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6061 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6121 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6181 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6241 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6301 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6361 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6421 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6481 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6541 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6601 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6661 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6721 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6781 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6841 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6901 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6961 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7021 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7081 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7141 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7201 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7261 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7321 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7381 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7441 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7501 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7561 ttacgccaag ctt
//
