LOCUS       62056_19995        7573 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector pSMXL6-LUC, complete sequence.
ACCESSION   MN728546
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..3000
                     /label=promotor of AtSMXL6
                     /note="promotor of AtSMXL6"
                     /regulatory_class="promoter"
     CDS             3008..4657
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      4678..4930
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4939..4955)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5429..5533
                     /label=AmpR promoter
     CDS             5534..6391
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6565..7153
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7441..7462
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7477..7507
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7515..7531
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7539..7555
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctgaaactta gggtttttcg aatttcggat tttccgatct gaggttttgg cagagagaca
       61 gagacggaga gtcgctgaca cgaaggtgac ggatgagaga tgattacagt ttcagtaaaa
      121 taaataatgg gctcatataa ggcctttaat ttatttattt ttattgtcca ggcccattta
      181 gttctttgaa ggttgcttta agaaaataac ttgtaaggcc tcgattggct taataatggg
      241 ctgatagaag gtttcgatgc caaaaaaggc atgacagagg tgacttgcca cttgtaactt
      301 gcccatgctg ctctccgcaa gtcttcacaa taatgtatac tatttgggaa ataatagttg
      361 ccataaagtt tggtttgctt catcgttagg cacatgtcga ccaaattttg gattcggggt
      421 agcaaacaaa caaaaacaat ttcactttga ttttcttttt ctctttttga ttttctaaat
      481 aatttttcaa tttatacata aatgaacata aatatatatt gttttcttga atatatcttt
      541 aatatgattt acagttactt ttgtagattt taaaataaag tgaataaaaa aagaagtaca
      601 aaacacattc actcaaaaag ctatgatatg acacaaatgt cacatcaaat atgcatgtga
      661 ttatctaaat gttcgacagg tgccactgaa ccatgagaat tagataacaa ttaatcaaag
      721 ataatcattc agggcttacg tattagaaaa ggattacgta taagaagata ataataggtt
      781 tgatttttat ctctggtaat tattcaattc aaaaagtttg atcaacatca ctggatttgt
      841 atgaaagttt tgtactttcc ttgaaaacca aagtggagtc tattcaaaga ccttttgctt
      901 gatattcaat atgacaatcg aggattggtt tacttggtct acgctatcca tcgaggacaa
      961 aattaatcat ctaagatgca tcacataaag tttatcaaca cgtggcataa aacgaatgga
     1021 tgttataaat aacgtcatcc acttataaag tcccacagtg tcaaaaggag accgcgaagg
     1081 ggaccacaga tcgaatagtg accgttggtt gccacgcgat ttacagatgt cgttagcttc
     1141 acttttggct ctaactaggg ctactgcgtg ctcgttacag gggatctgga actagggttc
     1201 acgctgatta tcgggtttta tttctgtatt atgttaatta tcaatgtgac ggtcaaaata
     1261 tgcctgtgag attaattaaa atttgttcct actatatttg tatatgcatt ctagtctctc
     1321 ctaagtaagt gtgacgctct taattaggat ttgatcattt aaggtggtgt cacgtatatt
     1381 tatctcccaa ctatttgaat tgaactactg cacacattaa tcagagactt ggcgaagttg
     1441 tgagaagttg aataatatac caataattaa atgcttagtt gcatatatat gtattatatg
     1501 gaacattata atcatgtatg ggttacaggg ttagtgtgga agaaacacga cgtgagcttt
     1561 aaccagctcg tggcgcaaat caaatcaaac tatttttggg tgtggttgtt ggactggtct
     1621 acttctagta gcagcttagc cgcgaaggtc gcaataccat aaacagaaaa ttatttcgat
     1681 gccttgtgat ttttggtgac ttctttccta acatacgtct taaaatacaa atgaccgaaa
     1741 ataggccatt ttaactttcc ggcagttgta aaaggatcga taattgagta aaaagatgcg
     1801 tctgcgatca ggaataaaag atgtgcaaaa gtaatgtgaa acaataaata aataaaaaat
     1861 cccctttatt agatgatata tatgacaatg acgcagaaaa gcatataaag gtggtgtttg
     1921 ggcctctaat cacttgtgct tttcctcttt tctcttatga cattcaccta atcttttata
     1981 aagtttaagc attgtctacc actcatactt tttatatatt ttctctacta tatttaagtt
     2041 gacttttctt tgtcaatccc aattaatttc tctttggatc atttttacta aatatgaaac
     2101 aattagtaga aagatgtaaa aaggggagag atgcgactcc ctcatgtttc aagaccttga
     2161 ctattagtat agtttgttca tataccatat gacctagaat ataagttatc agaaaaaaga
     2221 agaattattt aagggagaca aagatcaatt gtcgaatcaa gaagtcgtga aaaagcagtg
     2281 aaacaatttg atgggagaga acaaagaatc aaataattat attttagcac taatttctca
     2341 tgtcggtaaa ccttaaatta atcaaagtac tcaatcttgg taaataaagg tttgtgccat
     2401 acataatcaa agttatcaat cttgtacccc aatttatggt tgtaaaaact cttttaacat
     2461 gctaaaatct tatcatacag aactaaaagc tcgtaacatt aggagattga agcaaatgaa
     2521 taattgaaga gagaatcaga agatgtaaag tggggaaaga cgagagagat tctacgagca
     2581 ccacatattc gtattattat ttcaccatcg ttataagaca tgcaagaaag caaaaaaaac
     2641 aaaggagaat aaaagaaaaa aatcagacaa tatattggag gaccctacaa agcaatatac
     2701 caccaaaaca acgactcttg tacatatcca agcgtaagat tttgtggatt gaaccaaaac
     2761 catctttttt atatagcaac ttttaagcta aagagactaa taagaagttt tctgataaaa
     2821 atcatttcca gaaatttcac gcttcttttt tttttttata tatatttgct gattattttt
     2881 ttggcttttt cttgtgtttc tgattattgt tggtgagaag agtaagtggg ctggtttttt
     2941 attggagaaa aggcgttggt gaagtttcta cttttgattc tgactttttt tttgccggcg
     3001 gtcgaccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga
     3061 agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg
     3121 aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga
     3181 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat
     3241 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat
     3301 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat
     3361 gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa
     3421 cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta
     3481 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga
     3541 atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc
     3601 ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag
     3661 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt
     3721 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg
     3781 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca
     3841 ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag
     3901 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc
     3961 cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca
     4021 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa
     4081 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac
     4141 cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat
     4201 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct
     4261 acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct
     4321 gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt
     4381 gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg
     4441 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat
     4501 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt
     4561 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat
     4621 cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ttctagcgaa tttccccgat
     4681 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     4741 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     4801 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     4861 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     4921 ttactagatc gggaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4981 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5041 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     5101 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     5161 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5221 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5281 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5341 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5401 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5461 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5521 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5581 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5641 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5701 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5761 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5821 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5881 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5941 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6001 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6061 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6121 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6181 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6241 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6301 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6361 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6421 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6481 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6541 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6601 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6661 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6721 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6781 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6841 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6901 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6961 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7021 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7081 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7141 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7201 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7261 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7321 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7381 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7441 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7501 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7561 ttacgccaag ctt
//
