LOCUS       V000976                 5708 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000976
VERSION     V000976
KEYWORDS    pSMT3-M
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Carroll P, Schreuder LJ, Muwanguzi-Karugaba J, Wiles S, Robertson
            BD, Ripoll J, Ward TH, Bancroft GJ, Schaible UE, Parish T
  TITLE     Sensitive detection of gene expression in mycobacteria under
            replicating and non-replicating conditions using optimized far-red
            reporters.
  JOURNAL   PLoS One. 2010  . 5(3):e9823.
   PUBMED   20352111
REFERENCE   2  (bases 1 to 5708)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5708)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.
            2010  . 5(3):e9823."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(436..452)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3307..4302)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     rep_origin      4672..5260
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     5414..5431
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    5548..5569
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5584..5614
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5622..5638
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5646..5662
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 ctagaggtga ccacaacgac gcgcccgctt tgatcgggga cgtctgcggc cgaccattta
       61 cgggtcttgt tgtcgttggc ggtcatgggc cgaacatact cacccggatc ggagggccga
      121 ggacaaggtc gaacgagggg catgacccgg tgcggggctt cttgcactcg gcataggcga
      181 gtgctaagaa taacgttggc actcgcgacc ggtgagtcgt aggtcgggac ggtgaggcca
      241 ggcccgtcgt cgcagcgagt ggcagcgagg acaacttgag ccgtccgtcg cgggcactgc
      301 gcccggccag cgtaagtagc ggggttgccg tcacccggtg acccccggtt tcatccccga
      361 tccggaggaa tcacttcgca atggccaaga caattgcgga tcccccgggc tgcaggaatt
      421 cgatatcaag cttatcgata ccgtcgacct cgaggggggg cccggtacgt acccggggat
      481 catcgagccg agaacgttat cgaagttggt catgtgtaat cccctcgttt gaactttgga
      541 ttaagcgtag atacaccctt ggacaagcca gttggattcg gagacaagca aattcagcct
      601 taaaaagggc gaggcctgcg gtggtggaac accgcagggc ctctaaccgc tcgacgcgct
      661 gcaccaacca gcccgcgaac ggctggcagc cagcgtaagg cgcggctcat cgggcggcgt
      721 tcgccacgat gtcctgcact tcgagccaag cctcgaacac ctgctggtgt gcacgactca
      781 cccggttgtt gacaccgcgc gcggccgtgc gggctcggtg gggcggctgt gtcgcccttg
      841 ccagcgtgag tagcgcgtac ctcacctcgc ccaacaggtc gcacacagcc gattcgtacg
      901 ccataaagcc aggtgagccc accagctccg taagttcggg cgctgtgtgg ctcgtacccg
      961 cgcattcagg cggcaggggg tctaacgggt ctaaggcggc gtgtacggcc gccacagcgg
     1021 ctctcagcgg cccggaaacg tcctcgaaac gacgcatgtg ttcctcctgg ttggtacagg
     1081 tggttggggg tgctcggctg tcgctggtgt tccaccacca gggctcgacg ggagagcggg
     1141 ggagtgtgca gttgtggggt ggcccctcag cgaaatatct gacttggagc tcgtgtcgga
     1201 ccatacaccg gtgattaatc gtggtctact accaagcgtg agccacgtcg ccgacgaatt
     1261 tgagcagctc tggctgccgt actggccgct ggcaagcgac gatctgctcg aggggatcta
     1321 ccgccaaagc cgcgcgtcgg ccctaggccg ccggtacatc gaggcgaacc caacagcgct
     1381 ggcaaacctg ctggtcgtgg acgtagacca tccagacgca gcgctccgag cgctcagcgc
     1441 ccgggggtcc catccgctgc ccaacgcgat cgtgggcaat cgcgccaacg gccacgcaca
     1501 cgcagtgtgg gcactcaacg cccctgttcc acgcaccgaa tacgcgcggc gtaagccgct
     1561 cgcatacatg gcggcgtgcg ccgaaggcct tcggcgcgcc gtcgacggcg accgcagtta
     1621 ctcaggcctc atgaccaaaa accccggcca catcgcctgg gaaacggaat ggctccactc
     1681 agatctctac acactcagcc acatcgaggc cgagctcggc gcgaacatgc caccgccgcg
     1741 ctggcgtcag cagaccacgt acaaagcggc tccgacgccg ctagggcgga attgcgcact
     1801 gttcgattcc gtcaggttgt gggcctatcg tcccgccctc atgcggatct acctgccgac
     1861 ccggaacgtg gacggactcg gccgcgcgat ctatgccgag tgccacgcgc gaaacgccga
     1921 attcccgtgc aacgacgtgt gtcccggacc gctaccggac agcgaggtcc gcgccatcgc
     1981 caacagcatt tggcgttgga tcacaaccaa gtcgcgcatt tgggcggacg ggatcgtggt
     2041 ctacgaggcc acactcagtg cgcgccagtc ggccatctcg cggaagggag cagcgcgcac
     2101 ggcggcgagc acagttgcgc ggcgcgcaaa gtccgcgtca gccatggagg cattgctatg
     2161 agcgacggct acagcgacgg ctacagcgac ggctacaacc ggcagccgac tgtccgcaaa
     2221 aagcggcgcg tgaccgccgc cgaaggcgct cgaatcaccg gactatccga acgccacgtc
     2281 gtccggctcg tggcgcagga acgcagcgag tggctcgccg agcaggctgc acgccgcgaa
     2341 cgcatccgcg cctatcacga cgacgagggc cactcttggc cgcaaacggc caaacatttc
     2401 gggctgcatc tggacaccgt taagcgactc ggctatcggg cgaggaaaga gcgtgcggca
     2461 gaacaggaag cggctcaaaa ggcccacaac gaagccgaca atccaccgct gttctaacgc
     2521 aattggggag cgggtgtcgc gggggttccg tggggggttc cgttgcaacg ggtcggacag
     2581 gtaaaagtcc tggtagacgc tagttttctg gtttgggcca tgcctgtctc gttgcgtgtt
     2641 tcgttgcgcc cgttttgaat accagccaga cgagacgggg ttctacgaat cttggtcgat
     2701 accaagccat ttccgctgaa tatcggggag ctcaccgcca gaatcggtgg ttgtggtgat
     2761 gtacgtggcg aactccgttg tagtgcctgt ggtggcatcc gtggccactc tcgttgcacg
     2821 gttcgttgtg ccgttacagg ccccgttgac agctcaccga acgtagttaa aacatgctgg
     2881 tcaaactagg tttaccaacg atacgagtca gctcatctag ggccagttct aggcgttgtt
     2941 cgttgcgcgg ttcgttgcgc atgtttcgtg tggttgctag atggctccgc aaccacacgc
     3001 ttcgaggttg agtgcttcca gcacgggcgc gatccagaag aacttcgtcg tgcgactgtc
     3061 ctcgttgatc cttgccgagc tgggatggaa gctcggccga ccaccctgga ggagatgatc
     3121 gaggatgcca gggcctttca cgcccgccgc tgctgagcgt ccgccgccgg gcccgcaccg
     3181 ccgtcggccg gcccgctccg ggctcgcagc agcgggcttc ggcgcgggcc cggggctccc
     3241 gagcgcgggc ggggctccgg gcggccgccg ggggccgggg gcggcgccgg gcggcccggg
     3301 gcgtcaggcg ccgggggcgg tgtccggcgg cccccagagg aactgcgcca gttcctccgg
     3361 atcggtgaag ccggagagat ccagcggggt ctcctcgaac acctcgaagt cgtgcaggaa
     3421 ggtgaaggcg agcagttcgc gggcgaagtc ctcggtccgc ttccactgcg ccccgtcgag
     3481 cagcgcggcc aggatctcgc ggtcgccccg gaaggcgttg agatgcagtt gcaccaggct
     3541 gtagcgggag tctcccgcat agacgtcggt gaagtcgacg atcccggtga cctcggtcgc
     3601 ggccaggtcc acgaagatgt tggtcccgtg caggtcgccg tggacgaacc ggggttcgcg
     3661 gccggccagc agcgtgtcca cgtccggcag ccagtcctcc aggcggtcca gcagccgggg
     3721 cgagaggtag ccccacccgc ggtggtcctc gacggtcgcc gcgcggcgtt cccgcagcag
     3781 ttccgggaag acctcggaat ggggggtgag cacggtgttc ccggtcagcg gcaccctgtg
     3841 cagccggccg agcacccggc cgagttcgcg ggccagggcg agcagcgcgt tccggtcggt
     3901 cgtgccgtcc atcgcggacc gccaggtggt gccggtcatc cggctcatca ccaggtaggg
     3961 ccacggccag gctccggtgc cgggccgcag ctcgccgcgg ccgaggaggc ggggcaccgg
     4021 caccggggcg tccgccagga ccgcgtacgc ctccgactcc gacgcgaggc tctccggacc
     4081 gcaccagtgc tcgccgaaca gcttgatcac cgggtcgggc tcgccgacca gtacggggtt
     4141 ggtgctctcg ccgggcaccc gcagcaccgg cggcaccggc agcccgagct cctccagggc
     4201 tcggcgggcc agcggctccc agaattcctg gtcgttccgc aggctcgcgt aggaatcatc
     4261 cgaatcaata cggtcgagaa gtaacaggga ttcttgtgtc acagcggacc tctattcaca
     4321 gggtacgggc cggcttaatt ccgcacggcc ggtcgcgaca cggcctgtcc gcaccgcgga
     4381 tcaggcgttg acgatgacgg gctggtcggc cacgtcgggg acgacgggga gtcaggcaac
     4441 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4501 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4561 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4621 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4681 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4741 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4801 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4861 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4921 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4981 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5041 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5101 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5161 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5221 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5281 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5341 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5401 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5461 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5521 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5581 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5641 tcacacagga aacagctatg accatgatta ccagatctgg ctcgagctcc accgcggtgg
     5701 cggccgct
//
