LOCUS       40924_40742        1968 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Low-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize at HincII to make blunt cloning vector.
FEATURES             Location/Qualifiers
     source          1..1968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      65..96
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        97..187
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             188..1000
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1004..1192
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      1219..1246
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1258..1845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1867..1896)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gacgaattct ctagatatcg ctcaatactg accatttaaa tcatacctga cctccatagc
       61 agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa
      121 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      181 aggagctatg agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa
      241 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
      301 gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
      361 aggtagcgtt gccaatgatg ttacagatga gatggtcagg ctaaactggc tgacggaatt
      421 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
      481 cactgcgatc ccagggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
      541 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
      601 ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
      661 cggtttggtt ggtgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
      721 ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
      781 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
      841 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
      901 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
      961 gaataaattg cagtttcact tgatgctcga tgagtttttc taaatgacca aacaggaaaa
     1021 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
     1081 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
     1141 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgatgagg
     1201 gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt
     1261 ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc agaggtggcg
     1321 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1381 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1441 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1501 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1561 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1621 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1681 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctc
     1741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1801 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt
     1861 ttctaccgaa gaaaggccca cccgtgaagg tgagccagtg agttgattgc agtccagtta
     1921 cgctggagtc tgaggctcgt cctgaatgat atcaagcttg aattcgtt
//
