LOCUS       40924_40712        4801 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSM47, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Fuchs U, Czymmek KJ, Sweigard JA.
  TITLE     Five hydrophobin genes in Fusarium verticillioides include two 
            required for microconidial chain formation
  JOURNAL   Fungal Genet. Biol. 41 (9), 852-864 (2004)
  PUBMED    15288021
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Sweigard J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2003) Crop Genetics, DuPont, PO 80402, Wilmington,
            DE 19880-0402, USA
REFERENCE   3  (bases 1 to 4801)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4801)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2004"; volume: "41"; issue: "9"; pages: 
            "852-864"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2003) Crop Genetics, DuPont, PO 80402, Wilmington, DE 
            19880-0402, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    195..228
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(273..306)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        363..464
                     /label=TRP1 promoter
     CDS             465..1136
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1194..2536)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     complement(2580..2596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2604..2620)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2628..2658)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2673..2694)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2982..3570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3744..4601)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4602..4706)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaaca tatcataact tcgtataatg tatgctatac gaagttatag gcctggatct
      241 tcgaggtcga cggtatatcg aattaacata tcataacttc gtataatgta tgctatacga
      301 agttataggc ctggatcgcg gccgcattta aatggcgcgc ctcgaggcgc gccatttaaa
      361 tgaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg tgactattga
      421 gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct gttattaatt
      481 tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca gaggccgcag
      541 aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc aatagaaaga
      601 gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata
      661 gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag gaggatgttt
      721 tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat gagtcgtggc
      781 aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt ccaaaagact
      841 gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc ttgtttgatt
      901 cagaagcagg tgggacaggt gaacttttgg attggaactc gatttctgac tgggttggaa
      961 ggcaagagag ccccgaaagc ttacatttta tgttagctgg tggactgacg ccagaaaatg
     1021 ttggtgatgc gcttagatta aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga
     1081 caaatggtgt aaaagactct aacaaaatag caaatttcgt caaaaatgct aagaaatagg
     1141 ttattactga gtagtattta tttaagtatt gtttgtgcac ttgcctgcag cccaacgaag
     1201 catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
     1261 agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac
     1321 gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca
     1381 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta
     1441 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt
     1501 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc
     1561 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat
     1621 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc
     1681 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt
     1741 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta
     1801 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt
     1861 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat
     1921 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa
     1981 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt
     2041 gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg
     2101 tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag
     2161 ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa
     2221 cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac
     2281 ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt
     2341 atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct
     2401 cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta
     2461 gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt
     2521 cctttgatat tggatcatat gcatagtacc gagaaactag gaagcttggc gtaatcatgg
     2581 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2641 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2701 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2761 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     3181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3421 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4621 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4681 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     4741 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     4801 c
//
