LOCUS       40924_40677        9590 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSLS94, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Sanber KS, Knight SB, Stephen SL, Bailey R, Escors D, Minshull J, 
            Santilli G, Thrasher AJ, Collins MK, Takeuchi Y.
  TITLE     Construction of stable packaging cell lines for clinical lentiviral 
            vector production
  JOURNAL   Sci Rep 5, 9021 (2015)
  PUBMED    25762005
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   Sanber KS, Knight SB, Stephen SL, Bailey R, Escors D, Minshull J, 
            Santilli G, Thrasher AJ, Collins MK, Takeuchi Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2015) UCL Division of Infection and Immunity, 
            University College London, Gower Street, London WC1E 6BT, UK
REFERENCE   3  (bases 1 to 9590)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9590)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 5, 
            9021 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-JAN-2015) UCL Division of Infection and Immunity, University 
            College London, Gower Street, London WC1E 6BT, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2214..2247
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2270..2866
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    2969..3090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        3328..3706
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3707..3910
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          4046..4178
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             4214..5713
                     /codon_start=1
                     /label=HIV-1 gag
                     /note="gag protein from human immunodeficiency virus 1"
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVQAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPA
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFRNQRKIVKCFNCGKEG
                     HTARNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             5509..8517
                     /codon_start=1
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHT
                     NDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQ
                     AIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQDEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTIHTDNGSNFTSATVKAACWWAGIKQEFGIPYNPQSQGVVESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRNPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     polyA_signal    complement(8568..8689)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     8789..8822
                     /label=RE LoxP site
                     /note="RE LoxP site"
     protein_bind    complement(8789..8822)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(8898..8914)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8947..8965)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8975..8991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9133..9588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggc gccataactt
     2221 cgtataatgt atgctatacg aacggtaggg ccccccctcg agtgccacca tgaccgagta
     2281 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     2341 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     2401 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     2461 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     2521 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     2581 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     2641 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     2701 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     2761 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     2821 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     2881 cgacccgcag cgcccgaccg aaaggagtcg acgcacgacc ccatcactag tgaatttcga
     2941 cccgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     3001 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     3061 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     3121 caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     3181 aaaatcgatt caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     3241 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     3301 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     3361 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3421 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3481 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3541 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     3601 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     3661 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3721 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     3781 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     3841 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     3901 aagcagagct cgtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca
     3961 cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag
     4021 aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga
     4081 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     4141 tggtcttact gacatccact ttgcctttct ctccacaggt gctagcctcg agatccgaat
     4201 tccataggcc accatgggag cacgggctag tgttctttct ggaggtgagc ttgacaggtg
     4261 ggagaagatc agactgcgcc ccggcggcaa aaagaagtac aagctgaagc acatcgtgtg
     4321 ggcctctcgc gaattggaga ggtttgccgt gaaccccggg ctcctggaga caagcgaggg
     4381 gtgccggcag atcctcggcc aattgcagcc cagtttgcaa accggcagcg aggagttgcg
     4441 gagcctgtac aacaccgtgg ccacattgta ctgcgtccac cagcgcatcg aaatcaagga
     4501 tacaaaagag gccctggata aaatcgaaga ggaacagaat aagagcaaaa agaaggccca
     4561 acaagccgcc gctgataccg gccattctaa ccaagtgtct cagaactatc ccatcgtcca
     4621 aaatattcaa ggccagatgg tccaccaagc tatcagcccc cggaccctga acgcctgggt
     4681 gaaggtggtg gaggaaaaag ccttttctcc cgaggtcatc cctatgttca gcgccctgag
     4741 cgagggcgct acaccccagg acctgaatac aatgttgaat accgtcggcg gccaccaggc
     4801 cgctatgcag atgctgaagg aaacaattaa cgaagaggcc gccgagtggg accgggtcca
     4861 ccccgtccag gctggcccca tcgcacccgg gcaaatgcgg gagccgagag gctccgatat
     4921 cgccggcacc acctccacat tgcaagagca gatcggctgg atgaccaaca atcccccaat
     4981 tcccgtgggc gagatctaca agcggtggat cattctcggc ctgaacaaga tcgtgcggat
     5041 gtactctccc acatctatcc tcgatatccg gcagggcccc aaagagcctt tccgggatta
     5101 cgtggataga ttttacaaga ccttgcgggc tgaacaggcc agccaagaag tgaagaactg
     5161 gatgacggag acactcctcg tgcagaacgc caatcccgac tgcaaaacca tcctgaaggc
     5221 cttgggccca gccgccacct tggaggagat gatgaccgcc tgccaaggcg tgggaggccc
     5281 tgggcacaaa gcccgggtgc tcgccgaggc catgtctcag gtgaccaaca gcgccacaat
     5341 catgatgcaa cgggggaact tccgcaatca gcggaaaatc gtgaaatgct ttaactgtgg
     5401 caaagaaggg cacacagccc gcaactgcag ggcccctagg aaaaagggct gttggaaatg
     5461 tggaaaggaa ggacaccaaa tgaaagattg tactgagaga caggctaatt ttttagggaa
     5521 gatctggcct tcccacaagg gaaggccagg gaattttctt cagagcagac cagagccaac
     5581 agccccacca gaagagagct tcaggtttgg ggaagagaca acaactccct ctcagaagca
     5641 ggagccgata gacaaggaac tgtatccttt agcttccctc agatcactct ttggcagcga
     5701 cccctcgtca caataaagat aggggggcag ctcaaggagg ctctcctgga caccggagca
     5761 gacgacaccg tgctggagga gatgtcactc cctggtcggt ggaagcctaa gatgattggt
     5821 ggtatagggg gcttcattaa ggtgcggcaa tacgaccaaa tcttgatcga gatttgcggc
     5881 cacaaggcca tcggcaccgt gctggtgggc cccacccccg tgaatatcat cggccggaac
     5941 ctcctcaccc aaatcggctg taccctgaac ttccctatct ctcccatcga aaccgtgccc
     6001 gtgaagctga aacccggcat ggacgggccc aaggtgaagc agtggcccct caccgaggag
     6061 aagatcaagg ccctggtgga gatctgcacc gaaatggaga aagagggcaa gatcagcaag
     6121 atcggccccg agaaccccta taacaccccc gtgttcgcta tcaaaaagaa ggattccacc
     6181 aagtggcgga agctggtgga ctttcgggag ttgaacaaac ggacccagga tttttgggag
     6241 gtgcagctgg gcatccccca ccctgccggc ctgaagaaaa agaagagcgt gaccgtgctc
     6301 gacgtcggcg acgcctactt cagcgtgcct ctggacgagg attttcgcaa atacaccgcc
     6361 ttcacaatcc cctccatcaa taacgaaacc cccggcatcc ggtaccaata taacgtcttg
     6421 ccccaaggct ggaagggcag ccccgccatc tttcagtcct ctatgaccaa gattctggaa
     6481 cccttccgga agcagaaccc cgatatcgtg atttaccagt atatggacga cctctacgtg
     6541 ggcagcgatc tggagatcgg ccaacaccgg accaagatcg aagaactccg gcagcacctc
     6601 ctccgctggg gcttgacaac ccccgataag aagcaccaaa aggagcctcc ctttttgtgg
     6661 atgggctacg agttgcaccc cgacaagtgg accgtgcaac ccatcgtcct ccccgagaag
     6721 gattcttgga ccgtgaacga tatccaaaaa ctggtcggca agctcaactg ggcctcccaa
     6781 atctatcccg gcatcaaggt gcgccagctg tgcaagttgt tgcggggcac aaaggcgttg
     6841 accgaggtga tccccttgac cgaggaggcc gaattggagc tcgccgagaa tcgggaaatc
     6901 ttgaaggagc ccgtgcacgg cgtctactac gatcccagca aggatctgat cgccgagatc
     6961 caaaaacaag gccaggggca gtggacctac cagatctacc aggaaccctt caagaacctc
     7021 aagaccggca agtacgcccg gatgagaggc gcccatacca acgacgtgaa gcagctgacc
     7081 gaagccgtcc agaagatcac aaccgagtct atcgtgatct ggggcaaaac ccccaagttc
     7141 aagctcccta tccagaagga aacgtgggaa acctggtgga ccgaatactg gcaggctaca
     7201 tggattcccg aatgggagtt cgtgaacaca ccccctctgg tcaagctgtg gtatcaactg
     7261 gaaaaggagc ctatcgtggg cgccgagaca ttttacgtgg acggcgctgc caatcgcgaa
     7321 accaagctgg gcaaggccgg ctacgtgacc aatcggggcc gccagaaggt ggtgacattg
     7381 accgatacca ccaaccagaa aaccgaactg caggccatct acttggccct ccaagacagc
     7441 ggcctggagg tgaatatcgt gaccgatagc cagtacgccc tgggcattat ccaggcccag
     7501 cccgaccagt ccgagagcga actggtgaac cagatcatcg aacaactgat caagaaagag
     7561 aaagtgtacc tcgcctgggt gcccgcccat aaggggatcg gcggcaacga gcaggtggac
     7621 aagctggtgt ccgccggcat tcgcaaggtg ttgttcctgg acggcatcga caaagctcag
     7681 gacgagcacg aaaagtacca ttccaactgg cgggccatgg cctccgactt caatttgcca
     7741 cccgtggtgg ccaaggagat cgtggcttct tgcgacaagt gccaattgaa gggcgaggct
     7801 atgcacggcc aggtggattg ctcccccggc atctggcagt tggactgcac ccacctggag
     7861 ggcaaggtga ttctcgtggc cgtgcacgtg gcttccggct acatcgaggc tgaggtgatc
     7921 ccggccgaaa ccggccaaga gactgcctac ttcttgctga agctggccgg caggtggccc
     7981 gtaaagacca tccacaccga taacgggtct aactttacat ccgccaccgt gaaagctgct
     8041 tgctggtggg caggcattaa acaagagttc ggcatccctt ataaccctca gtcccagggc
     8101 gtggtggaga gcatgaacaa ggagctgaaa aagatcatcg gccaagtgcg ggaccaagcc
     8161 gagcacttga aaaccgccgt gcagatggcc gtgtttattc ataacttcaa gcggaagggc
     8221 ggcatcggcg gctattccgc cggtgagcgg atcgtggata tcatcgccac cgatatccag
     8281 accaaggagc tgcagaagca gatcaccaag atccagaact tcagagtgta ctatcgcgat
     8341 tctcggaacc ccttgtggaa ggggccagcc aaattgttgt ggaaggggga gggcgccgtg
     8401 gtgatccagg acaactccga tatcaaggtg gtcccgcgga ggaaggccaa aattatccgc
     8461 gactacggca agcaaatggc cggcgacgac tgcgtcgcct cccggcaaga cgaggactga
     8521 aagcttctcg acccgggatc ccgggcggcc gcttcgagca gacatgataa gatacattga
     8581 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     8641 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     8701 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta
     8761 aaacctctac aaatgtggta aaatcgatat aacttcgtat aatgtatgct atacgaacgg
     8821 taggcgccaa gcttgataga tctatttaaa tacgtagttt aaacctgagg tgaccatcga
     8881 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
     8941 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     9001 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     9061 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     9121 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     9181 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     9241 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     9301 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9361 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9421 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9481 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     9541 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
//
