LOCUS       40924_40672       11492 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSLS51, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Sanber KS, Knight SB, Stephen SL, Bailey R, Escors D, Minshull J, 
            Santilli G, Thrasher AJ, Collins MK, Takeuchi Y.
  TITLE     Construction of stable packaging cell lines for clinical lentiviral 
            vector production
  JOURNAL   Sci Rep 5, 9021 (2015)
  PUBMED    25762005
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   Sanber KS, Knight SB, Stephen SL, Bailey R, Escors D, Minshull J, 
            Santilli G, Thrasher AJ, Collins MK, Takeuchi Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2015) UCL Division of Infection and Immunity, 
            University College London, Gower Street, London WC1E 6BT, UK
REFERENCE   3  (bases 1 to 11492)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11492)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 5, 
            9021 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-JAN-2015) UCL Division of Infection and Immunity, University 
            College London, Gower Street, London WC1E 6BT, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        314..517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    738..937
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1138..1554
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     CDS             1598..2389
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     enhancer        2874..3253
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3254..3457
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             3609..4631
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4632..5348
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     repeat_region   5522..5969
                     /label=MLV U3
                     /note="MLV U3"
     protein_bind    5970..6003
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   6013..6080
                     /label=MLV R
                     /note="MLV R"
     repeat_region   6081..6157
                     /label=MLV U5
                     /note="MLV U5"
     promoter        complement(6414..6743)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             8297..8485
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    8590..8730
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8916..9504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9973..10354)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(10539..10643)
                     /label=AmpR promoter
     promoter        11433..11451
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tctagagtcc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
       61 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      121 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      181 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      241 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      301 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      361 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      421 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      481 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gcgccagtct
      541 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
      601 ccgaatcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccacga
      661 cgggggtctt tcatttgggg gctcgtccgg gatttggaga cccctgccca gggaccaccg
      721 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      781 tctatgtttg atgttatgcg cctgcgtctg tactagttag ctaactagct ctgtatctgg
      841 cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc
      901 agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg
      961 accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
     1021 tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc
     1081 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc
     1141 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc
     1201 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa
     1261 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc
     1321 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac
     1381 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac
     1441 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt
     1501 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggaatt
     1561 ccgatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     1621 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     1681 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     1741 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     1801 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     1861 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     1921 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     1981 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     2041 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     2101 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     2161 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     2221 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     2281 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     2341 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     2401 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     2461 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     2521 atcctccagc gcggggatct catgctggag ttcttcgccc accccgggct cgatcccctc
     2581 gcgagttggt tcagctgctg cctgaggctg gacgacctcg cggagttcta ccggcagtgc
     2641 aaatccgtcg gcatccagga aaccagcagc ggctatccgc gcatccatgc ccccgaactg
     2701 caggagtggg gaggcacgat ggccgctttg gtcgaggcgg atccggccat tagccatatt
     2761 attcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatcc
     2821 atatcataat atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg
     2881 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     2941 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3001 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3061 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3121 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3181 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3241 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     3301 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     3361 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3421 taggcatgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3481 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
     3541 ccgcgggccc tctagactcg agaattcacg cgtggtacct ctagaggatc cccggagccg
     3601 ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     3661 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     3721 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     3781 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     3841 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     3901 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     3961 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     4021 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     4081 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     4141 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     4201 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     4261 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     4321 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     4381 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     4441 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     4501 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     4561 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     4621 gggcaaagga aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4681 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4741 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4801 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     4861 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4921 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4981 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5041 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     5101 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5161 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5221 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5281 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5341 tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     5401 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     5461 aattgttgtt gttaacatcg ataaaataaa agattttatt tagtctccag aaaaaggggg
     5521 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     5581 catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa
     5641 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     5701 caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     5761 gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc
     5821 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     5881 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     5941 aataaaagag cccacaaccc ctcactcggt accgttcgta taatgtatgc tatacgaagt
     6001 tatgtttaaa cggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     6061 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     6121 tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga
     6181 gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag caacttatct
     6241 gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc ggtactagtt
     6301 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg gaacacccgg
     6361 ccgcaaccct gggagaggaa ttctcatgtt tgacagctta tcatcgataa gcttttgcaa
     6421 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
     6481 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     6541 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     6601 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     6661 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     6721 tttccacacc ctaactgaca cacattccac agccggatcc tctacgccgg acgcatcgtg
     6781 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     6841 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     6901 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     6961 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     7021 aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     7081 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     7141 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     7201 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     7261 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     7321 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     7381 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     7441 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     7501 ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca
     7561 tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg
     7621 cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta
     7681 acggattcac cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc
     7741 aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc
     7801 gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga
     7861 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
     7921 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
     7981 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
     8041 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
     8101 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
     8161 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
     8221 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aattccccct
     8281 tacacggagg catcaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat
     8341 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc
     8401 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg
     8461 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8521 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     8581 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     8641 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     8701 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     8761 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8821 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8881 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8941 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9001 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9061 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9121 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9181 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9241 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9301 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     9361 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9421 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9481 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9541 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9601 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9661 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9721 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9781 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9841 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9901 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9961 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
    10021 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10081 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg tcgttgtcag aagtaagttg
    10141 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10201 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10261 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10321 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10381 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10441 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10501 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10561 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10621 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    10681 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    10741 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    10801 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    10861 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    10921 catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
    10981 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
    11041 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
    11101 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
    11161 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc
    11221 tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag
    11281 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
    11341 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
    11401 taatacataa ccttatgtat catacacata cgatttaggt gacactatag aatacaagct
    11461 ggaagatctt ccagcttggg ctgcaggtcg ac
//
