LOCUS       V003147                 5920 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003147
VERSION     V003147
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Alrefaei YN, Suttiprapa S, Okatcha TI, Rinaldi G, Brindley PJ.
  TITLE     A plasmid encoding the Mos-1 mariner transposase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Alrefaei YN, Suttiprapa S, Okatcha TI, Rinaldi G, Brindley PJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2011) Microbiology, Immunology and Tropical
            Medicine, George Washington University, 2300 Eye Street, NW,
            Washington, DC 20037, USA
REFERENCE   3  (bases 1 to 5920)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-SEP-2011) Microbiology, Immunology and Tropical Medicine, George
            Washington University, 2300 Eye Street, NW, Washington, DC 20037,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..485
                     /label="hr5 enhancer"
                     /note="baculovirus early transcription enhancer"
     promoter        489..1080
                     /label="IE1 promoter"
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     CDS             1107..2141
                     /gene="marinerT"
                     /label="Mariner Mos1 transposase"
                     /note="Mariner Mos1 transposase from Drosophila mauritiana.
                     Accession#: Q7JQ07"
     terminator      2652..2959
                     /label="IE1 terminator"
                     /note="terminator of the ie1 gene from the baculovirus
                     Autographa californica"
     promoter        3735..3839
                     /label="AmpR promoter"
     CDS             3840..4697
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4871..5459
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5747..5768
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5783..5813
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5821..5837
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5845..5861
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcgagt ggatgatggt
      121 catttgtttt tcaaatcgag atgatgtcat gttttgcaca cgggctcata aactgcttta
      181 cgagtagaat tctacgtgta acgcacgatc gattgatgag tcatttgttt tgcaatatga
      241 tatcatacaa tatgactcat ttgtttttca aaaccgaact tgatttacgg gtagaattct
      301 acttgtaaag cacaatcaaa aagatgatgt catttgtttt tcaaaactga actcgcttta
      361 cgagtagaat tctacgtgta aaacacaatc aagaaatgat gtcatttgtt ataaaaataa
      421 aagctgatgt catgttttgc acatggctca taactaaact cgctttacgg gtagaattct
      481 acgcgcgtcg atgtctttgt gatgcgcgcg acatttttgt aggttattga taaaatgaac
      541 ggatacgttg cccgacatta tcattaaatc cttggcgtag aatttgtcgg gtccattgtc
      601 cgtgtgcgct agcatgcccg taacggacct cgtacttttg gcttcaaagg ttttgcgcac
      661 agacaaaatg tgccacactt gcagctctgc atgtgtgcgc gttaccacaa atcccaacgg
      721 cgcagtgtac ttgttgtatg caaataaatc tcgataaagg cgcggcgcgc gaatgcagct
      781 gatcacgtac gctcctcgtg ttccgttcaa ggacggtgtt atcgacctca gattaatgtt
      841 tatcggccga ctgttttcgt atccgctcac caaacgcgtt tttgcattaa cattgtatgt
      901 cggcggatgt tctatatcta atttgaataa ataaacgata accgcgttgg ttttagaggg
      961 cataataaaa gaaatattgt tatcgtgttc gccattaggg cagtataaat tgacgttcat
     1021 gttggatatt gtttcagttg caagttgaca ctggcggcga caagatcgtg aacaaccaag
     1081 tgaccgcgga tctagatgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc
     1141 ggacagtatt aattttctgt tttcatttga agaaaacagc tgcggaatcg caccgaatgc
     1201 ttgttgaagc ctttggcgaa caagtaccaa ctgtgaaaac gtgtgaacgg tggtttcaac
     1261 gcttcaaaag tggtgatttt gacgtcgacg acaaagagca cggaaaaccg ccaaaaaggt
     1321 acgaagacgc cgaactgcaa gcattattgg atgaagacga tgctcaaacg caaaaacaac
     1381 tcgcagagca gttggaagta agtcaacaag cagtttccaa tcgcttgcga gagatgggaa
     1441 agattcagaa ggtcggtaga tgggtgccac atgagttgaa cgagaggcag atggagaggc
     1501 gcaaaaacac atgcgaaatt ttgctttcac gatacaaaag gaagtcgttt ttgcatcgta
     1561 tcgttactgg agatgaaaaa tggatctttt ttgttaatcc taaacgtaaa aagtcatacg
     1621 ttgatcctgg acaaccggcc acatcgactg ctcgaccgaa tcgctttggc aagaagacga
     1681 tgctctgtgt ttggtgggat cagagcggtg tcatttacta tgagctcttg aaacccggcg
     1741 aaacggtgaa tacggcacgc taccaacaac aattgatcaa tttgaaccgt gcgcttcaga
     1801 gaaaacgacc ggaatatcaa aaaagacaac acagggtcat ttttctccat gacaacgctc
     1861 catcacatac ggcaagagcg gttcgcgaca cgttggaaac actcaattgg gaagtgcttc
     1921 cgcatgcggc ttactcacca gacctggccc catccgatta ccacctattc gcttcgatgg
     1981 gacacgcact cgctgagcag cgcttcgatt cttacgaaag tgtgaaaaaa tggctcgatg
     2041 aatggttcgc cgcaaaagac gatgagttct actggcgtgg aatccacaaa ttgcccgaga
     2101 gatgggaaaa atgtgtagct agcgacggca aatactttga ataaatggat ccaaaaaaca
     2161 aaaaaagtac gctcacgtac aaatacagca gcgtcgctaa tcttttgttt aataattata
     2221 aatatcatga caatattgcg agtaataata acgcagaaaa tttaaaaaag gttaagaagg
     2281 aggacggcag catgcacatt gtcgaacagt atttgactca gaatgtagat aatgtaaagg
     2341 gtcacaattt tatagtattg tctttcaaaa acgaggagcg attgactata gctaagaaaa
     2401 acaaagagtt ttattggatt tctggcgaaa ttaaagatgt agacgttagt caagtaattc
     2461 aaaaatataa tagatttaag catcacatgt ttgtaatcgg taaagtgaac cgaagagaga
     2521 gcactacatt gcacaataat ttgttaaaat tgttagcttt aatattacag ggtctggttc
     2581 cgttgtccga cgctataacg tttgcggaac aaaaactaaa ttgtaaatat aaaaaattcg
     2641 aatttaatta attatacata tattttgaat ttaattaatt atacatatat tttatattat
     2701 ttttgtcttt tattatcgag gggccgttgt tggtgtgggg ttttgcatag aaataacaat
     2761 gggagttggc gacgttgctg cgccaacacc acctcctcct cctcctttca tcatgtatct
     2821 gtagataaaa taaaatatta aacctaaaaa caagaccgcg cctatcaaca aaatgatagg
     2881 cattaacttg ccgctgacgc tgtcactaac gttggacgat ttgccgacta aaccttcatc
     2941 gcccagtaac caatctagac ccaagtcgcc aactaaatca ccaaacgagt aaggttcgat
     3001 gcacatgagt gtttggcccg caggaagatc gctaatatct acgtattgag gcgaatctgg
     3061 gtcggcggac ggatcgctgc cgcgacaaac tgttttttct acttcatagt tgaatccttg
     3121 gcacatgttg gttagttcgg gcggattgtt aggcaacaag gggtcgaatg ggcaaatggt
     3181 aacatccgac tgatttagat tggggtcttg acgacaagtg cgctgcaata acaagcaggc
     3241 ctcggcgatt tctccggcgt ctttaccttg cacataataa cttccgccgg tgttattgat
     3301 ggcgttgatt atatcttgta ctagtgtggc ggcgctaaac aagaaatagc cgccggtggc
     3361 caagagtatg cccgttcctc ctacttttaa gctggcgcgc ctagccggcc cagctgcgcg
     3421 aaattttaac aaaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     3481 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     3541 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3601 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     3661 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3721 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3781 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3841 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3901 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3961 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4021 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4081 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4141 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4201 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4261 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4321 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4381 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4441 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4501 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4561 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4621 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4681 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4741 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4801 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4861 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4921 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4981 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     5041 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5101 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5161 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5221 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5281 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5341 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5401 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5461 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5521 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5581 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5641 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5701 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5761 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5821 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattggcgc
     5881 gcctaggccg gccgaattcg aatggccatg ggacgtcggg
//
