LOCUS       V003148                 8609 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003148
VERSION     V003148
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Mesen-Ramirez P, Reinsch F, Blancke Soares A, Bergmann B, Ullrich
            AK, Tenzer S, Spielmann T.
  TITLE     Stable Translocation Intermediates Jam Global Protein Export in
            Plasmodium falciparum Parasites and Link the PTEX Component EXP2
            with Translocation Activity
  JOURNAL   PLoS Pathog. 12 (5), E1005618 (2016)
   PUBMED   27168322
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Birnbaum J, Flemming S, Spielmann T.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Parasitology Section, Bernhard Nocht
            Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, Hamburg
            20359, Germany
REFERENCE   3  (bases 1 to 8609)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8609)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS
            Pathog."; date: "2016"; volume: "12"; issue: "5"; pages: "E1005618"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-MAR-2016) Parasitology Section, Bernhard Nocht Institute for
            Tropical Medicine, Bernhard-Nocht-Str. 74, Hamburg 20359, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..453
                     /label="P. berghei 3' region"
                     /note="P. berghei 3' region"
                     /regulatory_class="terminator"
     regulatory      complement(465..810)
                     /label="Pfhop 5' region"
                     /note="Pfhop 5' region"
                     /regulatory_class="terminator"
     regulatory      811..1301
                     /label="PfCAM 5' region"
                     /note="PfCAM 5' region"
                     /regulatory_class="promoter"
     CDS             1308..1868
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     regulatory      1874..2443
                     /label="P. falciparum hrpII 3' region"
                     /note="P. falciparum hrpII 3' region"
                     /regulatory_class="terminator"
     promoter        complement(2448..2465)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2472..2488)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2962..3066
                     /label="AmpR promoter"
     CDS             3067..3924
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4098..4686
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4974..4995
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5010..5040
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5048..5064
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5072..5088
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5106..5124
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    5142..6257
                     /note="similar to PfEHD; P. falciparum Eps 15 homology
                     domain protein, C-terminal fragment (162-533)"
     misc_feature    6264..6320
                     /label="linker"
                     /note="linker"
     misc_feature    6327..6980
                     /note="similar to 2xFKBP; 2 x FKBP (FK506 binding protein)"
     CDS             6330..6650
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label="FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6657..6977
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label="FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    6987..7025
                     /label="linker"
                     /note="linker"
     CDS             7032..7745
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     misc_feature    7752..7808
                     /label="T2A sequence"
                     /note="T2A sequence"
     CDS             7755..7808
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7809..8600
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ggatatggca gcttaatgtt cgtttttctt atttatatat ttataccaat tgattgtatt
       61 tataactgta aaaatgtgta tgttgtgtgc atattttttt ttgtgcatgc acatgcatgt
      121 aaatagctaa aattatgaac attttatttt ttgttcagaa aaaaaaaact ttacacacat
      181 aaaatggcta gtatgaatag ccatatttta tataaattaa atcctatgaa tttatgacca
      241 tattaaaaat ttagatattt atggaacata atatgtttga aacaataaga caaaattatt
      301 attattatta ttatttttac tgttataatt atgtgtctcc ttcaatgatt cataaatagt
      361 tggacttgat ttttaaaatg tttataatat gattagcata gttaaataaa aaaagttgaa
      421 aaattaaaaa aaaacatata aacacaaatg atggtttttc cttcaatttc gatatcaatt
      481 tatagaaaca aaatatatac ttgtataatt ttattttttt atataaatca ttacatatat
      541 aattatacaa tattttttct aagagataat tatatattaa tatatataaa aaaaggtgtt
      601 tttttttttt ttttttattt ttatttttat tttatggtaa tattttattt tccttatttt
      661 ataaattata ttagtttata tgtgattaat tttatatatt atcaatttat atatttttaa
      721 atgcttactt aattatcttt tttttttttt tttttttttt ttcccctctt tttatattaa
      781 tttatttttg aaaaaattga tatatatata tatatataat atatatatat acatgtagta
      841 gtattaaaca atgtataata tatataaata atatatttat atatttcatt tcaattttaa
      901 ttttttttgg tttttttttt ttttcttttt gtcatattta aaaaaaatta tattcatata
      961 agttatgcat tttttataaa cattattcaa tatatgtata atataatata tatatatata
     1021 ttaatgtatt attccaatgt gcatgataaa agaaaaaaat aatatttata aaaaaaaaga
     1081 aaaataaaac aaaaaaagaa aaaaaaaaaa aaaaaaaaaa aaatacaaaa ataaataata
     1141 taatttataa ttatatattc ttgtcacaat aaaaatatat atatatatat atatatttat
     1201 aatatgtata ttttaaacta gaaaaggaat aactaatatt ttatttatta tcattcaaga
     1261 tttatatttt ataataataa atacctaata gaaatatatc aggatccatg catggttcgc
     1321 taaactgcat cgtcgctgtg tcccagaaca tgggcatcgg caagaacggg gactacccct
     1381 ggccaccgct caggaacgaa tttagatatt tccagagaat gaccacaacc tcttcagtag
     1441 aaggtaaaca gaatctggtg attatgggta agaagacctg gttctccatt cctgagaaga
     1501 atcgaccttt aaagggtaga attaatttag ttctcagcag agaactcaag gaacctccac
     1561 aaggagctca ttttctttcc agaagtctag atgatgcctt aaaacttact gaacaaccag
     1621 aattagcaaa taaagtagac atggtctgga tagttggtgg cagttctgtt tataaggaag
     1681 ccatgaatca cccaggccat cttaaactat ttgtgacaag gatcatgcaa gactttgaaa
     1741 gtgacacgtt ttttccagaa attgatttgg agaaatataa acttctgcca gaatacccag
     1801 gtgttctctc tgatgtccag gaggagaaag gcattaagta caaatttgaa gtatatgaga
     1861 agaatgatta agcttattta ataatagatt aaaaatatta taaaaataaa aacataaaca
     1921 cagaaattac aaaaaaaata catatgaatt ttttttttgt aatcttcctt ataaatatag
     1981 aataatgaat catataaaac atatcattat tcatttattt acatttaaaa ttattgtttc
     2041 agtatcttta atttattatg tatatataaa aataacttac aattttatta ataaacaata
     2101 tatgtttatt aattcatgtt ttgtaattta tgggatagcg atttttttta ctgtctgtat
     2161 ttttcttttt taattatgtt ttaattgtat tttattttta ttattgttct ttttatagta
     2221 ttattttaaa acaaaatgta ttttctaaga acttataata ataataaata taaattttaa
     2281 taaaaattat atttatcttt tacaatatga acataaagta caacattaat atatagcttt
     2341 taatattttt attcctaatc atgtaaatct taaatttttc tttttaaaca tatgttaaat
     2401 atttatttct cattatatat aagaacatat ttattaaatc tagaattcta tagtgagtcg
     2461 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2521 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2581 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     2641 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     2701 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     2761 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     2821 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     2881 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2941 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3001 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3061 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3121 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3181 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3241 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3301 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3361 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3421 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3481 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3541 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3601 acgatgcctg tagcaatgcc aacaacgttg cgcaaactat taactggcga actacttact
     3661 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3721 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3781 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3841 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3901 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3961 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4021 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4081 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4141 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4201 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4261 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4321 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4381 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4441 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4501 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4561 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4621 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4681 tcgaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4741 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4801 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4861 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4921 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4981 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5041 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     5101 aagctattta ggtgacacta tagaatactc gcggccgcta agtattaagt ggaataaagc
     5161 aaataagtcg tggttacgat ttcgaaaaag taatatattg gtttgcacaa cgagttgatt
     5221 taatattatt aatttttgat gctcataaat tagatatatc tgatgaattt agaagatgta
     5281 tacaagcaat taaaggacaa gattctaaaa ttcgtataat attaaataaa gcggatacaa
     5341 taaatactca acaattaatg agagtttatg gttcattaat gtggtcttta ggtattgtta
     5401 taaatacccc agaagttaat agggtatata taggatcctt ttgggataaa aaattaatgc
     5461 atgatgaaaa tagaacaata tttgaagaag aagcttctga tttatataaa gaaatttcga
     5521 aaattcctag aaattctaca atggttagat taaatgattt tattaaaaga tgtcgaactt
     5581 taaaagttca tatatattta ttaacacact taagaaaaaa attaccattt tttaaaaaat
     5641 tcttaaataa aagaaaaatt gtaaattctt tagaaaaaat atatgaggag gtatctaaag
     5701 attataattt accactggga gatttccctc ctgtacaatt tatgaaagaa aaattattag
     5761 atatggattg gatgagaatt cctaaattag aaacaaaaaa aatcgatagg ataaataaag
     5821 ttttaaatac acatatacca caattattag aaatgattcc gaaagaatct gttactgttg
     5881 aagtgtcaag atttgaaaat caagaaggta caattgtcga aaataaacta acaccatttt
     5941 tagagttaac atcaggagaa atacccttat gggtaaaaca aaaatattta ttaagcccaa
     6001 ttgatacttc aaaatattct gatgattttt ataaactagg tccagatgat tttggaaaat
     6061 tatcaggaga acaagttaaa caagatctaa taaaaagtaa attaccaagt gccgttctac
     6121 ataaaatatg gaatttggcg gatataacag aagatggata tctcgatctt ttcgaatatt
     6181 ccttagctag acatttcata gaaatgaaaa ttgaggggtt cgacttacct gccaaggttc
     6241 ccaaggatat tattaaacct aggtcaggat tgagatcaag atctgctgct gctggtgctg
     6301 gtggtgctgc tagagctgct ctgcagagag gagtacaagt tgaaacaata tcaccaggag
     6361 atggtcgtac atttccaaaa agaggtcaaa cttgtgttgt acattatact ggaatgcttg
     6421 aagatggaaa gaaatttgat tcatctcgtg atagaaataa accatttaaa tttatgctag
     6481 gtaaacaaga agtaatacga ggttgggaag aaggagttgc tcaaatgagt gtaggtcaaa
     6541 gagcaaaact tactatatct ccagattatg cttatggtgc aactggacat ccaggtataa
     6601 ttccacctca tgcaactctt gtatttgatg tggagcttct aaaactagaa actagaggtg
     6661 ttcaggttga aacaatttca cctggagatg gcagaacctt tcctaaaaga ggacagactt
     6721 gcgtagttca ttatacaggc atgctagagg atggtaagaa atttgattct agtcgagata
     6781 gaaataagcc attcaagttt atgctaggta aacaggaagt aataagaggt tgggaagagg
     6841 gtgtagcaca gatgtcagtt ggacaaagag caaagttaac aatatcacca gattatgcat
     6901 acggtgcaac aggccatcct ggcatcatcc ctccacatgc aactttagta ttcgacgttg
     6961 aattgttaaa gttagagaca acgcgtgcta gaggtgctgc tgctggtgct ggaggtgcag
     7021 gtagacgtac gatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     7081 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     7141 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     7201 ggccaacact tgtcactact ttcgcgtatg gtcttcaatg ctttgcgaga tacccagatc
     7261 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     7321 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     7381 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     7441 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     7501 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     7561 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     7621 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     7681 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgagctct
     7741 acaaagtcga cggagaagga agaggaagtt tattaacatg tggagatgta gaagaaaatc
     7801 caggaccaat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7861 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7921 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7981 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     8041 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     8101 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     8161 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     8221 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     8281 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     8341 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     8401 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8461 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8521 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8581 gccttcttga cgagttcttc taactcgag
//
