LOCUS       pSLCAR-CD19-28z        8866 bp DNA     circular SYN 15-APR-2024
DEFINITION  Modular CD28-CD3z CAR backbone, For Transient Expression or 
            Lentiviral Production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Bloemberg D, Nguyen T, MacLean S, Zafer A, Gadoury C, Gurnani K, 
            Chattopadhyay A, Ash J, Lippens J, Harcus D, Page M, Fortin A, Pon 
            RA, Gilbert R, Marcil A, Weeratna RD, McComb S
  TITLE     A High-Throughput Method for Characterizing Novel Chimeric Antigen 
            Receptors in Jurkat Cells.
  JOURNAL   Mol Ther Methods Clin Dev. 2020 Jan 31;16:238-254. doi: 
            10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13.
  PUBMED    32083149
REFERENCE   2  (bases 1 to 8866)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8866)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther
            Methods Clin Dev."; date: "2020-01-31"; pages: "
            10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..71)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        243..622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        623..825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             840..1020
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1067..1192
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1685..1918
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2103..2147
                     /codon_start=1
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2370..2487
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2513..2724
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             2750..2803
                     /codon_start=1
                     /label=signal peptide from CD28
                     /note="signal peptide from CD28"
                     /translation="MLRLLLALNLFPSIQVTG"
     CDS             2813..2878
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2879..3196
                     /codon_start=1
                     /label=anti-CD19 VL from FMC63
                     /translation="DIQMTQTTSSLSASLGDRVTISCRASQDISKYLNWYQQKPDGTVK
                     LLIYHTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKL
                     EI"
     CDS             3254..3613
                     /codon_start=1
                     /label=anti-CD19 VH FMC63
                     /translation="EVKLQESGPGLVAPSQSLSVTCTVSGVSLPDYGVSWIRQPPRKGL
                     EWLGVIWGSETTYYNSALKSRLTIIKDNSKSQVFLKMNSLQTDDTAIYYCAKHYYYGGS
                     YAMDYWGQGTSVTVSS"
     CDS             3629..3763
                     /codon_start=1
                     /label=CD8a hinge region
                     /translation="TTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACD
                     "
     CDS             3773..3868
                     /codon_start=1
                     /label=CD28 transmembrane region
                     /note="CD28 transmembrane region"
                     /translation="PSKPFWVLVVVGGVLACYSLLVTVAFIIFWVR"
     CDS             3869..3988
                     /codon_start=1
                     /label=CD28 costim.
                     /translation="SKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRS"
     CDS             3995..4336
                     /codon_start=1
                     /label=CD3z stim domains
                     /translation="LRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPE
                     MGGKPQRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYD
                     ALHMQALPPR"
     CDS             4343..4399
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4400..5113
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    5131..5719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5791..6024
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6102..6236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6263..6398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6419..6437)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6447..6463)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6605..7060
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7086..7190
                     /label=AmpR promoter
     CDS             7191..8048
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8222..8810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 catgtgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
       61 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
      121 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
      181 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      241 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      601 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      661 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      721 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      781 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
      841 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      901 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      961 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1021 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1081 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1141 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1201 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1261 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1321 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1381 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1441 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1501 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc ccgggtttat tacagggaca
     2341 gcagagatcc actttggcgc cggctcgagt tttaaaagaa aaggggggat tggggggtac
     2401 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2461 aaacaaatta caaaaattca aaatttttcg agtggctccg gtgcccgtca gtgggcagag
     2521 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     2581 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2641 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2701 aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgccacca tgctcaggct
     2761 gctcttggct ctcaacttat tcccttcaat tcaagtaaca ggagggtctt cggactacaa
     2821 ggatcatgac ggagactata aggatcacga tattgattac aaagatgacg acgacaaaga
     2881 catccagatg acacagacta catcctccct gtctgcctct ctgggagaca gagtcaccat
     2941 cagttgcagg gcaagtcagg acatctctaa gtatttgaat tggtatcagc agaaaccaga
     3001 tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag tcccatcaag
     3061 gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc tggagcaaga
     3121 agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt tcggaggggg
     3181 gactaagttg gaaataacag gctccacctc tggatccggc aagcccggat ctggcgaggg
     3241 atccaccaag ggcgaggtga aactgcagga gtcaggacct ggcctggtgg cgccctcaca
     3301 gagcctgtcc gtcacatgca ctgtctcagg ggtctcatta cccgactatg gtgtaagctg
     3361 gattcgccag cctccacgaa agggtctgga gtggctggga gtaatatggg gtagtgaaac
     3421 cacatactat aattcagctc tcaaatccag actgaccatc atcaaggaca actccaagag
     3481 ccaagttttc ttaaaaatga acagtctgca aactgatgac acagccattt actactgtgc
     3541 caaacattat tactacggtg gtagctatgc tatggactac tggggtcaag gaacctcagt
     3601 caccgtctcc tcagcggccg caggtaccac cacaacgccc gctcctcggc caccgacgcc
     3661 agcgccaact attgcgagtc agcctctcag tctgcgacct gaggcttgtc gaccagcagc
     3721 cggaggcgca gtgcacacga gggggctgga cttcgcctgt gatagaagac ctccttctaa
     3781 gcccttttgg gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac
     3841 agtggccttt attattttct gggtgaggag taagaggagc aggctcctgc acagtgacta
     3901 catgaacatg actcccaggc ggcccggacc cacccgcaag cattaccagc cctatgcccc
     3961 accacgcgac ttcgcagcct atcgctccgc tagcctgaga gtgaagttca gcaggagcgc
     4021 agacgccccc gcgtaccagc agggccagaa ccagctctat aacgagctca atctaggacg
     4081 aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa
     4141 gccgcagaga aggaagaacc ctcaggaagg cctgtacaat gaactgcaga aagataagat
     4201 ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc cggaggggca aggggcacga
     4261 tggcctttac cagggtctca gtacagccac caaggacacc tacgacgccc ttcacatgca
     4321 ggccctgccc cctcgcgcta gcgccacgaa cttctctctg ttaaagcaag caggcgacgt
     4381 ggaagaaaac cccggtcccg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4441 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4501 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4561 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     4621 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4681 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4741 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4801 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4861 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4921 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4981 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5041 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5101 cgagctgtac aagtaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     5161 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5221 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5281 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5341 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5401 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5461 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5521 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5581 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5641 ctgccggctc tgcggcctct tccgcgtctt tgccttcgcc ctcagacgag tcggatctcc
     5701 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     5761 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat
     5821 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5881 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5941 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6001 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     6061 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     6241 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6301 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6361 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     6421 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     6481 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     6541 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     6601 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6661 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6721 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6781 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6841 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6901 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6961 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7021 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7081 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7141 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7201 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7261 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7321 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7381 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7441 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7501 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7561 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7621 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7681 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7741 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7801 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7861 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7921 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7981 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8041 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8101 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8161 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8221 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8281 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8341 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8401 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8461 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8521 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8581 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8641 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8701 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8761 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8821 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctca
//
