LOCUS       62056_19935        7668 bp DNA     circular SYN 18-NOV-2020
DEFINITION  Cloning vector pSL1180 EACMCV DNA-B 1.6mer, complete sequence.
ACCESSION   MT856192
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Deeply Sequenced Infectious Clones of Key Cassava Begomovirus 
            Isolates from Cameroon
  JOURNAL   Microbiol Resour Announc 9 (46), e00802-20 (2020)
  PUBMED    33184153
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2020) Ecology, Evolution, and Natural Resources, 
            Rutgers University, 14 College Farm Rd, New Brunswick, NJ 08901, USA
REFERENCE   3  (bases 1 to 7668)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc"; date: "2020"; volume: "9"; issue: "46"; pages: 
            "e00802-20"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-AUG-2020) Ecology, Evolution, and Natural Resources, Rutgers 
            University, 14 College Farm Rd, New Brunswick, NJ 08901, USA"
FEATURES             Location/Qualifiers
     source          1..7668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1047..1068
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1083..1113
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1121..1137
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1145..1161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1242..2924
                     /note="duplicated part of EACMCV DNA-B partial tandem
                     dimer, BclI to ClaI, inclusive, copy 1"
     misc_feature    1495..4226
                     /label=EACMCV DNA-B monomer unit, nick site to nick site
                     /note="EACMCV DNA-B monomer unit, nick site to nick site"
     gene            1801..2565
                     /gene="BV1"
                     /label=BV1
     CDS             1801..2565
                     /codon_start=1
                     /transl_table=11
                     /gene="BV1"
                     /product="NSP"
                     /label=BV1
                     /protein_id="QNR00640.1"
                     /translation="MYTTPRRGYRSAYRPRAIRRNYAKTPYKPVTKPPVNRRITFDSSK
                     QVYVRRSIEDVHSGANMQLANQGQQTSYVNFPSLGVDGNGGRCFDHIKLLNLRVSGTVS
                     VTQIGGDDPMGEQSLLRGIFLMAVIVDKRPFVPEGVNTLPTFKELFGEYDTVYGNPRLK
                     DNIRHRYRLLGTVKQYITTEEAHVQKPVNLRRKLSNARYPVWSSFKDLDASSTGGNYKN
                     VNKNAILVSYVWVSCERSKCDVYAQFVLNYVG"
     gene            complement(2706..3629)
                     /gene="BC1"
                     /label=BC1
     CDS             complement(2706..3629)
                     /codon_start=1
                     /transl_table=11
                     /gene="BC1"
                     /product="MP"
                     /label=BC1
                     /protein_id="QNR00641.1"
                     /translation="MDNQFTVTDNTYIHSKRTEYVLTNDAAPIHLQFPSSFEQATMRLK
                     GRCMKIDHIIIEYRNQVPFNATGSVIVEIRDNRVSLEDAAQAAFTFPIACNVDLHYFSS
                     TYFSISEPSPWSIMYRVEDSNVVEGVKFASIKAKLRLSSAKHSTDIRFKPPTINILSKG
                     YTKDCIDFWSVEKGETRRRLLNPTPTAHSQRPIAHRPITILPGETWATKSQLGLPCSSS
                     QRDIGCMRSQSMRIDPTTRPLDADNDSTEYPYQGLYRLSTPVLDPGDSVSQTPSDTVSR
                     KDLESLIETTINRCLIKTQCETPRQL"
     misc_feature    2925..3973
                     /note="unique part of EACMCV DNA-B partial tandem dimer,
                     ClaI to BclI, noninclusive"
     misc_feature    3974..5656
                     /note="duplicated part of EACMCV DNA-B partial tandem
                     dimer, BclI to ClaI, inclusive, copy 2"
     primer_bind     complement(5737..5753)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5966..6421
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6703..6807
                     /label=AmpR promoter
     CDS             6808..7665
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
       61 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      961 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1021 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     1081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     1141 cacacaggaa acagctatga catgattacg aattcgaatg gccatgggac gtcgacctga
     1201 ggtaattata acccgggccc tatatatgga tccaattgca atgatcagat gtgatgcgcc
     1261 gtgtcgttaa agtatatcgt attgggtctt tatataggcg ttaataagtg acacgtggta
     1321 gagagagaaa gaagagagaa gcgagagcat tcgggggaca ctcaaaactc cagggaaacg
     1381 gtggaacggg gggcaatata tatgatgtcc cccaatggca tatgtgtaaa taggtagact
     1441 tccatttgaa ttgaaaagta ggatctgtgt aattgcggcc atccgattaa tattaccgga
     1501 tggccgcttt ggtggacgcg tggacgttgg cgaatgggtg ggtccgacag aaagcgttag
     1561 tgcgttccag cagatggaaa aggagagcat gtggtcctgt agacattttt attataatga
     1621 aattgcgcga gtgttgtact gacacgtggc aagagggatt gttaatatca ttgtcgtggt
     1681 gggtccgtta aatagatgtt aatgggttta aggactggta tattggggag ccacgtgtat
     1741 gcatgatagt gtctcagatg tgtataaata tccgagcatg ttaaggggtt ctaccacacc
     1801 atgtatacta cgcctaggcg tgggtataga tcagcgtata ggcctcgtgc catacgtcga
     1861 aattatgcga aaacgccgta taaaccggta acgaaaccgc ccgttaacag aaggataaca
     1921 tttgattcgt cgaaacaggt gtatgtgagg aggtcgatag aggatgtgca cagtggggcg
     1981 aatatgcagt tggctaatca gggtcagcaa acgtcgtatg tgaactttcc atcgctcgga
     2041 gttgatggta atgggggtag gtgtttcgat cacattaagc ttttaaactt acgggtgtcg
     2101 gggaccgtta gcgtcacgca aattggagga gacgatccta tgggagagca gtctctattg
     2161 aggggtattt ttttaatggc cgttattgtg gacaaaagac catttgtccc cgaaggtgtc
     2221 aacacgttgc ctacgtttaa ggaattattt ggagagtatg ataccgtcta cggcaatcct
     2281 aggttaaagg ataatattcg acatcgttat cgtctgcttg ggaccgttaa acagtatata
     2341 actacggagg aagctcatgt acaaaagccg gtgaatttaa gacgaaagtt aagtaacgcc
     2401 cggtatcccg tgtggtcttc gttcaaggat ttggatgcta gtagtactgg gggtaattat
     2461 aagaatgtta acaagaatgc tattcttgtg agttatgtat gggtgtcttg cgaacgtagc
     2521 aagtgtgacg tgtatgcaca atttgtatta aattatgtgg gatgataata aaaggtttta
     2581 attgtgcgca atatcaacca tttggagcaa gtgataacag tgtggacata tagataaatg
     2641 tatgtgttgt aaacgaaata tgattaaggg gggaaacaaa accagtaaat acaaaataca
     2701 tatatctaca actgtcgcgg tgtctcgcat tgagttttaa tgaggcatcg gttaatggtt
     2761 gtctcgatga gggattcaag gtccttgcgt gagactgtgt ccgatggtgt ttgtgacact
     2821 gaatcgccag ggtctaatac tggtgtgctt aatctataga gtccttgata tgggtattcg
     2881 gttgagtcgt tgtccgcgtc tagtggtcgt gtggtcggat cgatgcgcat cgactgtgat
     2941 cgcatacatc ctatgtcccg ttggcttgat gagcatggta ggccgagttg tgatttggta
     3001 gcccatgttt cgccaggtag aatggttatg ggcctgtggg cgatgggtct ttgactatgg
     3061 gctgttggag tcggatttag taatctccgt cttgtttccc ctttttccac ggaccagaag
     3121 tctatgcaat cttttgtgta tcccttggat aatatgttga ttgttggggg cttgaatcgt
     3181 atgtccgtgg aatgtttggc cgatgataat cggagcttcg ccttgatgga tgcgaatttc
     3241 acgccttcga cgacgttaga gtcttcgact ctgtacatga tgctccaagg ggagggttct
     3301 gaaatcgaaa aatatgttga agagaagtag tggaggtcta cgttgcaagc gattgggaaa
     3361 gtgaatgcag cttgagctgc gtcttcaagg ctgacgcgat tgtctcggat ttcgacgatg
     3421 accgatccag ttgcgttaaa tgggacctgg tttcggtatt caattataat gtggtcgatt
     3481 ttcatacatc ggcctttgag tcgcatggta gcctgctcga atgagctagg gaattggaga
     3541 tgaatgggtg cagcatcgtt tgttaataca tactcggtgc gtttgctgtg tatgtaagta
     3601 ttgtcggtga cggtgaattg gttgtccatg ctaagaagta aaaaacaagg gtgaggaaac
     3661 ggatagaaga gatgtataaa agtgagaaca aggttagaaa atgtttagag acatggaaac
     3721 aaataggcat ttgagacata taaaaacata ctaaatcaga acaattatca tatatgtatg
     3781 actggccgcg cagcggaatg gaaggcagat aaatcggcga acaaagaaaa aaagcgaagt
     3841 gggttatgtg atgtaaatca cttacggaat caccggtgaa gcagtcgtga aggaatgcct
     3901 cggccaaggg cgagaaaaca aaaaataaat gttaaggaaa taaaagttga acgtattggg
     3961 ataaaaagga aagtgatcag atgtgatgcg ccgtgtcgtt aaagtatatc gtattgggtc
     4021 tttatatagg cgttaataag tgacacgtgg tagagagaga aagaagagag aagcgagagc
     4081 attcggggga cactcaaaac tccagggaaa cggtggaacg gggggcaata tatatgatgt
     4141 cccccaatgg catatgtgta aataggtaga cttccatttg aattgaaaag taggatctgt
     4201 gtaattgcgg ccatccgatt aatattaccg gatggccgct ttggtggacg cgtggacgtt
     4261 ggcgaatggg tgggtccgac agaaagcgtt agtgcgttcc agcagatgga aaaggagagc
     4321 atgtggtcct gtagacattt ttattataat gaaattgcgc gagtgttgta ctgacacgtg
     4381 gcaagaggga ttgttaatat cattgtcgtg gtgggtccgt taaatagatg ttaatgggtt
     4441 taaggactgg tatattgggg agccacgtgt atgcatgata gtgtctcaga tgtgtataaa
     4501 tatccgagca tgttaagggg ttctaccaca ccatgtatac tacgcctagg cgtgggtata
     4561 gatcagcgta taggcctcgt gccatacgtc gaaattatgc gaaaacgccg tataaaccgg
     4621 taacgaaacc gcccgttaac agaaggataa catttgattc gtcgaaacag gtgtatgtga
     4681 ggaggtcgat agaggatgtg cacagtgggg cgaatatgca gttggctaat cagggtcagc
     4741 aaacgtcgta tgtgaacttt ccatcgctcg gagttgatgg taatgggggt aggtgtttcg
     4801 atcacattaa gcttttaaac ttacgggtgt cggggaccgt tagcgtcacg caaattggag
     4861 gagacgatcc tatgggagag cagtctctat tgaggggtat ttttttaatg gccgttattg
     4921 tggacaaaag accatttgtc cccgaaggtg tcaacacgtt gcctacgttt aaggaattat
     4981 ttggagagta tgataccgtc tacggcaatc ctaggttaaa ggataatatt cgacatcgtt
     5041 atcgtctgct tgggaccgtt aaacagtata taactacgga ggaagctcat gtacaaaagc
     5101 cggtgaattt aagacgaaag ttaagtaacg cccggtatcc cgtgtggtct tcgttcaagg
     5161 atttggatgc tagtagtact gggggtaatt ataagaatgt taacaagaat gctattcttg
     5221 tgagttatgt atgggtgtct tgcgaacgta gcaagtgtga cgtgtatgca caatttgtat
     5281 taaattatgt gggatgataa taaaaggttt taattgtgcg caatatcaac catttggagc
     5341 aagtgataac agtgtggaca tatagataaa tgtatgtgtt gtaaacgaaa tatgattaag
     5401 gggggaaaca aaaccagtaa atacaaaata catatatcta caactgtcgc ggtgtctcgc
     5461 attgagtttt aatgaggcat cggttaatgg ttgtctcgat gagggattca aggtccttgc
     5521 gtgagactgt gtccgatggt gtttgtgaca ctgaatcgcc agggtctaat actggtgtgc
     5581 ttaatctata gagtccttga tatgggtatt cggttgagtc gttgtccgcg tctagtggtc
     5641 gtgtggtcgg atcgatacgc gtacgtcgcg accgcggaca tgtacagagc tcgagaagta
     5701 ctagtggcca cgtgggccgt gcaccttaag cttggcactg gccgtcgttt tacaacgtcg
     5761 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5821 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5881 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     5941 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6001 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6061 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6121 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6181 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6241 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6301 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6361 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     6421 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     6481 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6541 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6601 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6661 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6721 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6781 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6841 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6901 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6961 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7021 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7081 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7141 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7201 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7261 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7321 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7381 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7441 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7501 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7561 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7621 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaa
//
