LOCUS       62056_19930        4758 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..31
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        94..112
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        885..903
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     primer_bind     complement(1745..1761)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1974..2429
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2711..2815
                     /label=AmpR promoter
     CDS             2816..3673
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3847..4435
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4723..4744
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgaca tgattacgaa ttctaatacg actcactata gggttaaaac
      121 agcctgtggg ttgatcccac ccacagggcc tattgggcgc tagcactctg gtatcacggt
      181 acctttgtgc gcctgtttta tatcccctcc cccaactgta acttagaagt aacacactcc
      241 gatcaacagt cagcgtggca caccagccat gttttgatca agcacttctg ttaccccgga
      301 ctgagtatca atagactgct cacgcggttg aaggagaaag cgttcgttat ccggccaact
      361 acttcgaaaa acccagtaac accatagagg ttgcagagtg tttcgctcag cactacccca
      421 gtgtagacca ggccgatgag tcaccgcatt ccccacgggc gaccgtggcg gtggctgcgt
      481 tggcggcctg cctatgggga aacccatagg acgctctaat acagacatgg tgcgaagagt
      541 ctattgagct agttggtaat cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca
      601 cacaccctca aaccagaggg cagtgtgtcg taacgggcaa ctctgcagcg gaaccgacta
      661 ctttgggtgt ccgtgtttca ttttattcct atactggctg cttatggtga caattgagag
      721 attgttacca tatagctatt ggattggcca tccggtgtct aatagagcta ttatatatct
      781 ctttgttgga tttataccac ttagcttgag agaggttaaa acattacaat tcattgttaa
      841 attgaataca acaaaggatc ctaaggtacc taattgccta gaaaacatga ggatcaccca
      901 tgtctgcagg tcgactctag aaaacatgag gatcacccat gtctgcagta ttcccgggtt
      961 cattagatcc taaggtacct aattgcctag aaaacatgag gatcacccat gtctgcaggt
     1021 cgactctaga aaacatgagg atcacccatg tctgcagtat tcccgggttc attagatcct
     1081 aaggtaccta attgcctaga aaacatgagg atcacccatg tctgcaggtc gactccagaa
     1141 aacatgagga tcacccatgt ctgcagtatt cccgggttca ttagatccta aggtacctaa
     1201 ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg actctagaaa acatgaggat
     1261 cacccatgtc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
     1321 acatgaggat cacccatgtc tgcaggtcga ctctagaaaa catgaggatc acccatgtct
     1381 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaaa catgaggatc
     1441 acccatgtct gcaggtcgac tccagaaaac atgaggatca cccatgtctg cagtattccc
     1501 gggttcatta gatctgcgcg cgatcgatat cagcgcttta aatttgcgca tgctagctat
     1561 agttctagag gtaccggttg ttaacgttag ccggctacgt atactccgga atattaatag
     1621 gcctaggatg catatggcgg ccgcctgcag ctggcgccat cgatacgcgt acgtcgcgac
     1681 cgcggacatg tacagagctc gagaagtact agtggccacg tgggccgtgc accttaagct
     1741 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     1801 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     1861 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     1921 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     1981 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2041 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2101 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2161 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     2221 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2281 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     2341 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2401 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     2461 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2521 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2581 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     2641 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     2701 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2761 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2821 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2881 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2941 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3001 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3061 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3121 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3181 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3241 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3301 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3361 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3421 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3481 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3541 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3601 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3661 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3721 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3781 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3841 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3901 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3961 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4021 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4081 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4141 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4201 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4261 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4321 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4381 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4441 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4501 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4561 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4621 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     4681 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     4741 tcattaggca ccccaggc
//
