LOCUS       V016342                 6903 bp    DNA     circular SYN 26-JUN-2019
DEFINITION  Exported.
ACCESSION   V016342
VERSION     V016342
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Aoki SK, Lillacci G, Gupta A, Baumschlager A, Schweingruber D,
            Khammash M.
  TITLE     A universal biomolecular integral feedback controller for robust
            perfect adaptation
  JOURNAL   Nature 570, 533-537 (2019)
   PUBMED   31217585
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Aoki SK, Lillacci G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2019) Biosystems Science and Engineering, ETH
            Zurich, Mattenstrasse 26, Basel 4058, Switzerland
REFERENCE   3  (bases 1 to 6903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 570,
            533-537 (2019)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-APR-2019) Biosystems Science and Engineering, ETH Zurich,
            Mattenstrasse 26, Basel 4058, Switzerland"
FEATURES             Location/Qualifiers
     source          1..6903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      26..47
                     /label="UP element"
                     /note="UP element"
                     /regulatory_class="enhancer"
     regulatory      50..85
                     /label="PsigW"
                     /note="PsigW"
                     /regulatory_class="promoter"
     regulatory      112..118
                     /label="sRBS"
                     /note="sRBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             128..169
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             176..1048
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     regulatory      1139..1145
                     /label="sRBS"
                     /note="sRBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             1155..1178
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1191..1901
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     terminator      2206..2292
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2384..2411
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(2462..2509)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(2513..3262)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     regulatory      complement(3291..3344)
                     /label="Pconstitutive"
                     /note="Pconstitutive"
                     /regulatory_class="promoter"
     regulatory      3395..3459
                     /label="Plux"
                     /note="Plux"
                     /regulatory_class="promoter"
     regulatory      3395..3414
                     /label="LuxR binding site"
                     /note="LuxR binding site"
                     /regulatory_class="other"
     regulatory      3460..3485
                     /label="RBS5000"
                     /note="RBS5000"
                     /regulatory_class="ribosome_binding_site"
     CDS             3486..4046
                     /gene="sigW"
                     /label="ECF RNA polymerase sigma factor SigW"
                     /note="ECF RNA polymerase sigma factor SigW from Bacillus
                     subtilis (strain 168). Accession#: Q45585"
     regulatory      4061..4147
                     /label="rrnB T1 terminator"
                     /note="rrnB T1 terminator"
                     /regulatory_class="terminator"
     regulatory      4174..4245
                     /label="PBAD"
                     /note="PBAD"
                     /regulatory_class="promoter"
     regulatory      4174..4190
                     /label="AraC I1 binding site"
                     /note="AraC I1 binding site"
                     /regulatory_class="other"
     regulatory      4195..4211
                     /label="AraC I2 binding site"
                     /note="AraC I2 binding site"
                     /regulatory_class="other"
     regulatory      4261..4286
                     /label="RBS5000"
                     /note="RBS5000"
                     /regulatory_class="ribosome_binding_site"
     CDS             4287..4550
                     /codon_start=1
                     /transl_table=11
                     /product="RsiW"
                     /label="RsiW"
                     /note="cytoplasmic RsiW domain"
                     /protein_id="QDD67909.1"
                     /translation="MSCPEQIVQLMHMHLDGDILPKDEHVLNEHLETCEKCRKHFYEME
                     KSIALVRSTSHVEAPADFTANVMAKLPKEKKRASVKRWFRTH"
     terminator      complement(4768..4811)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(5074..5662)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(5750..5844)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(5877..6734)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6735..6839)
                     /label="AmpR promoter"
ORIGIN
        1 gacgtctcta caccctgcca aaaatggaaa attttttttc aaaagtaaaa ttgaaacctt
       61 ttgaaacgaa gctcgtatac atacagagga tcctgtcaat ttccgcgata gaggaggtag
      121 atctatgggc aaaccgattc cgaacccgct gctgggcctg gatagcaccg ccgcggctga
      181 agcgcaaaat gatcccctgc tgccgggata ctcgtttaat gcccatctgg tggcgggttt
      241 aacgccgatt gaggccaacg gttatctcga tttttttatc gaccgaccgc tgggaatgaa
      301 aggttatatt ctcaatctca ccattcgcgg tcagggggtg gtgaaaaatc agggacgaga
      361 atttgtttgc cgaccgggtg atattttgct gttcccgcca ggagagattc atcactacgg
      421 tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt tactttcgtc cgcgcgccta
      481 ctggcatgaa tggcttaact ggccgtcaat atttgccaat acggggttct ttcgcccgga
      541 tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa atcattaacg ccgggcaagg
      601 ggaagggcgc tattcggagc tgctggcgat aaatctgctt gagcaattgt tactgcggcg
      661 catggaagcg attaacgagt cgctccatcc accgatggat aatcgggtac gcgaggcttg
      721 tcagtacatc agcgatcacc tggcagacag caattttgat atcgccagcg tcgcacagca
      781 tgtttgcttg tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag ggattagcgt
      841 cttaagctgg cgcgaggacc aacgtatcag ccaggcgaag ctgcttttga gcaccacccg
      901 gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac gatcaactct atttctcgcg
      961 ggtatttaaa aaatgcaccg gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa
     1021 agtgaatgat gtagccgtca agttgtcact cgaggcagca aataaaaatg aagagaatac
     1081 gaatgaagtt gcacaaccaa tgttaaatgc aggtcaagca aatcgccgtc gcgtttaaag
     1141 gaggtatata tatggactac aaggacgacg acgacaaggc cgccatcgag cgtaaaggcg
     1201 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
     1261 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
     1321 agttcatctg tactactggt aaactgccgg taccttggcc gactctggta acgacgctga
     1381 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
     1441 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
     1501 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
     1561 tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg gaatacaatt
     1621 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
     1681 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
     1741 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
     1801 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
     1861 ccgcagcggg catcacgcat ggtatggatg aactgtacaa agcggccgcg gcgaacaaaa
     1921 acgaagaaaa caccaacgaa gtgccgacct ttatgctgaa cgcgggccag gcgaaccgtc
     1981 ttcagttgta agcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga
     2041 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     2101 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     2161 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     2221 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     2281 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     2341 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     2401 gatggccttt ttgcgtttct acaaactctg gcggtaagtc aatgatagca ctcggtacct
     2461 ccaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt taatttttaa
     2521 agtatgggca atcaattgct cctgttaaaa ttgctttaga aatactttgg cagcggtttg
     2581 ttgtattgag tttcatttgc gcattggtta aatggaaagt gacagtacgc tcactgcaac
     2641 ctaatatttt tgaaatatcc caagagcttt ttccttcgca tgcccacgct aaacattctt
     2701 tttctctttt ggttaaatcg ttgtttgatt tattatttgc tatatttatt tttcgataat
     2761 tatcaactag agaaggaaca attaatggta tgttcataca cgcatgtaaa aataaactat
     2821 ctatatagtt gtctttttct gaatgtgcaa aactaagcat tccgaagcca ttgttagccg
     2881 tatgaatagg gaaactaaac ccagtgataa gacctgatgt tttcgcttct ttaattacat
     2941 ttggagattt tttatttaca gcattgtttt caaatatatt ccaattaatt ggtgaatgat
     3001 tggagttaga ataatctact ataggatcat attttattaa attagcgtca tcataatatt
     3061 gcctccattt tttagggtaa ttatctagga ttgaaatatc agatttaacc atagaatgag
     3121 gataaatgat cgcgagtaaa taatattcac aatgtaccat tttagtcata tcagataagc
     3181 attgattaat atcattattg cttctacaag ctttaatttt attaattatt ctgtatgtgt
     3241 cgtcggcatt tatgtttttc atattcacca ccctgaattg actctcttcc gggcgctatc
     3301 atgccatacc gcgaaaggtt ttgcgccatt cgatggcgcg ccgcttcgtc aggccacata
     3361 gctttcttgt tctgatcgga acgatcgttg gctgacctgt aggatcgtac aggtttacgc
     3421 aagaaaatgg tttgttatag tcgaataaat actagagaac ttctagatcg ctaaaacggg
     3481 ctagcatgga aatgatgatt aaaaaaagaa ttaaacaagt caaaaaaggc gaccaggatg
     3541 catttgcgga catcgtagat atttacaaag ataaaattta tcagctttgc taccgtatgc
     3601 ttggcaatgt gcatgaggcg gaggatattg cacaggaagc tttcatcaga gcgtacgtta
     3661 atatcgacag ttttgatatt aaccggaaat tttcaacttg gctttatcga atcgcgacca
     3721 atttgaccat tgaccgcatt cgcaaaaaga agccggatta ttacctcgac gcagaggtgg
     3781 ctggtacgga aggcttgacc atgtattctc aaatcgtcgc agatggtgtt ttgcctgaag
     3841 atgcagttgt atcgctggag ctctcaaaca cgatacagca gaaaatttta aagcttcctg
     3901 acaaatacag aacagtcatc gtattaaagt atattgacga actctcatta attgaaatcg
     3961 gggagattct aaacattcct gtggggactg tgaaaacgcg gattcacaga ggcagagagg
     4021 ctcttagaaa acaattaagg gatctttaat ctagaggcat caaataaaac gaaaggctca
     4081 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     4141 gacaaatgac gaagcaacat tacactagtc gactagcatt tttatccata agattagcgg
     4201 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttgga
     4261 aataacgcaa agggaggcgg atcgatatga gctgtcctga acaaattgtg cagcttatgc
     4321 atatgcatct tgatggagat atccttccaa aagatgaaca cgtattaaat gaacatctgg
     4381 agacatgcga gaaatgcaga aagcattttt acgagatgga gaaatccata gcgctcgtac
     4441 ggagcacatc gcatgtcgaa gcccccgcgg attttaccgc taatgtcatg gcaaaattgc
     4501 ctaaggagaa gaaaagagct tctgtaaaaa gatggttcag aacccattaa gcatgcaagc
     4561 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
     4621 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
     4681 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
     4741 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     4801 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     4861 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     4921 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     4981 caaactctga attctctaga tactacatca cagatgacag gcaaaaggcc agcaaaaggc
     5041 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5101 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5161 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5221 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5281 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5341 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5401 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5461 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     5521 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5581 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5641 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5701 gtggaacgaa aactcacgtt aagggatttt ggtcatgact agtgcttgga ttctcaccaa
     5761 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     5821 ttactggatc tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa
     5881 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5941 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6001 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6061 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6121 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6181 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6241 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6301 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6361 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6421 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6481 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6541 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6601 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6661 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6721 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6781 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6841 acatttcccc gaaaagtgcc acctgacggc tgtctgttca gttatgaacg aaaagtgcca
     6901 cct
//
