LOCUS       40924_40582        6267 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSK9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Kuchmina E, Wallner T, Kryazhov S, Zinchenko VV, Wilde A.
  TITLE     An expression system for regulated protein production in 
            Synechocystis sp. PCC 6803 and its application for construction of a
            conditional knockout of the ferrochelatase enzyme
  JOURNAL   J. Biotechnol. 162 (1), 75-80 (2012)
  PUBMED    22749908
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Zinchenko VV, Kryazhov SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2011) Dept. of Genetics, Biology Division, Moscow 
            State University, Moscow 119899, Russia
REFERENCE   3  (bases 1 to 6267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol."; date: "2012"; volume: "162"; issue: "1"; pages: 
            "75-80"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-DEC-2011) Dept. of Genetics, Biology Division, Moscow State 
            University, Moscow 119899, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    656..1145
                     /note="p6803-platform; derived from Synechocystis sp. PCC
                     6803 chromosome in INSD: BA000022"
     CDS             complement(1819..2475)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     misc_feature    2581..3058
                     /note="leader region of petJ gene derived from
                     Synechocystis sp. PCC 6803 chromosome in INSD: BA000022"
     misc_feature    3059..4063
                     /note="p6803-platform; derived from Synechocystis sp. PCC
                     6803 chromosome in INSD: BA000022"
     promoter        complement(4082..4100)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4121..4137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4145..4161)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4169..4199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4214..4235)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4523..5111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5285..6142)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6143..6247)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggaggccag
      661 tgctggagga atatgatttt gtcatcctcg actgtgcccc tggttataat ctgttgaccc
      721 gcagtggcat tgcggccagc gacttttatc tgttgccggc tcgtcctgaa cccctatcgg
      781 tggtggggat gcagttactg gaaagaagaa ttgagaaact gaaggaaagc cataaggcct
      841 ccgatgatcc cctgaatatc aatctgatcg gagtggtgtt tattctgtcc ggcggcggtt
      901 tgatgagtcg ctactataac caggtaatgc ggcgggtaca aacggatttc accccgggac
      961 aactttttca gcagtccatt cccatggatg tcaatgtggc taaggcagtg gatagcttta
     1021 tgccggtggt tacctccatg cccaatacgg cgggttcaaa agcttttatt aaattaaccc
     1081 aggaattttt acagaaagta gaagcttttg gctaaagcaa agcccccatt gattaacaac
     1141 gggaggggta ccgaggtgct gctgaagttg cccgcaacag agagtggaac caaccggtga
     1201 taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt
     1261 acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt
     1321 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc
     1381 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac
     1441 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
     1501 tgtattaacg aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata
     1561 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
     1621 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
     1681 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
     1741 cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata
     1801 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1861 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg
     1921 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1981 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     2041 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     2101 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     2161 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     2221 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     2281 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     2341 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     2401 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     2461 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     2521 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacct cggtacccct
     2581 catcgggggc tgtgttggcc gagacggcac tgaggatttt actctccatg gcattccaag
     2641 gaatatctac ccaactcacc tgctccggcg gattgttccg ctcaaaagta ctaatcaagt
     2701 cgtcaaaata cttattaaat tttggctgca attgcatagt ccaaaagctg actttcccct
     2761 ccatgctctg gggggaattg ctctggcaac tgattaatcc actgagcaac agcccaagac
     2821 acgcaaacaa aaaccaacgt cttggcgatc gccatcggca ccatgaaacc atcgtaaaag
     2881 ctggggaaag aataaaaaac agtggttcag gaattgcatt gccatggcca cttcacaaac
     2941 ctagccaatt ttagcttgac cgcaactttg acagattgtc ttttgacttt gcctggaccg
     3001 cctcccataa taccttcgcg tcttgaagac tttatccttg aaaggagaac atatgtttct
     3061 cggcaaaaat taattatcga ttggctggaa cctggtcaaa ccagggcttt tcatccattg
     3121 gaaaagcgat tttgatcatc tagggtcagg agcaaagatc tgatcaaata ttgatcattt
     3181 attaggaaag ctgaactttc accactttat ttttggcttc ctctactttg ggcaaagtca
     3241 aagttaggat accggcatcg taattagctt taacttctgt gttttggatt gctccaggta
     3301 caggaataac ccggcggaaa ctgccatagc ggaactctgt gcgccgcacc ccatcttttt
     3361 cggtgctatg ggtatcctgg cgatcgccgc tgacggtcac cgcatccctg gcggcttgga
     3421 tgtccaaatt atcggggtcc atgccaggta attctagttt gagcacatag gcttcttcag
     3481 tttcagttag ttctgcttta ggattaaacc cttggcgatc gccgtggcgg tccgtaggga
     3541 caaaaacttc ttcaaacagt tggttcatct gctgctggaa attatccatt tcccgcaggg
     3601 gattgtaaag aatgagagac ataatgttaa ctcctgatgt gtggaaggaa ttgattaccc
     3661 ttgaatggtt ctatcttaaa atttcccctt ccaggttaga ttcggttttc aggaaagaag
     3721 gtggggggat tgccgaaatt acatttctag ccgcaatttt tagtaaaaaa aagatgagtt
     3781 tttacctcac cttaagtaaa tatttgagtg gcaaaacaaa atggtaaaaa tagctaagct
     3841 tccaccgccc tatggatttt tggaaggaag tcttaggttg tgaaaaacta taaaaaccaa
     3901 ccataggaat ggagaccttt acccaacaag ttgaccccta ggtaacaaat ccaaaccacc
     3961 gtaaaaccgc tggcggccaa aatagcgggc ttgcggcctt gccaaccttt ggtaatgcgg
     4021 gcatggagat aggcggcaaa tactagccag gtgattaggg cccggtaccc agcttttgtt
     4081 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4141 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4201 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4261 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4321 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4381 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4441 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4501 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4561 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4621 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4681 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4741 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4801 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4861 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4921 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     4981 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5041 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5101 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5161 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5221 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5281 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5341 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5401 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5461 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5521 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5581 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5641 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5701 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5761 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5821 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5881 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5941 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6001 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6061 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6121 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6181 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6241 ttccgcgcac atttccccga aaagtgc
//
