LOCUS       V003156                 6615 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003156
VERSION     V003156
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Stable low-copy-number Staphylococcus aureus shuttle vectors
  JOURNAL   Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)
   PUBMED   12634346
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2002) Biological Sciences, University of Sydney,
            Macleay Building, A12, Sydney, NSW 2006, Australia
REFERENCE   3  (bases 1 to 6615)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6615)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-NOV-2002) Biological Sciences, University of Sydney, Macleay
            Building, A12, Sydney, NSW 2006, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..37
                     /note="multiple cloning site (MCS); contains uniques sites
                     for HindIII, SalI, BamHI and SmaI"
     terminator      complement(114..200)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             468..1115
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1373..2110)
                     /codon_start=1
                     /gene="par"
                     /product="partitioning protein"
                     /label="par"
                     /note="Par"
                     /protein_id="AAO24777.1"
                     /translation="MKTIKMVADELNVTKQTVVNNAKNLNISFEKENGVNYIDDNDYLK
                     IVEKITKKERTTQNKENKKSEITYENTEKNRYNNSDGFETLKTKVNELEKQVEIFETRA
                     KNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLEHQLEEERQLSYSF
                     DKSTNDRENFDVQEASYTSDSVNTDQYQKEEKKPEVQPKDISESQQDEKSQQQDDSFNQ
                     NDKDIAIEETQTKKGFWSRLFGG"
     gene            complement(1373..2110)
                     /gene="par"
                     /label="par"
     CDS             2535..3455
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label="rep"
                     /note="Rep"
                     /protein_id="AAO24778.1"
                     /translation="MSKFKKISASEFETLRFYQLPKFLFEDEYFSKMPTDAKVMYALLK
                     DRFELSRLNNWVDSENNIYLLYTNKQLCSILNYAEPKIIKLKKELEKYNLIINERQGLN
                     KPNKIYLLEPTYDKELINSKFQNKEFISSRTNKSSVQELINSKSSDTDFNNTEYIETKN
                     NDTNYTNDTSNMISKNSHSNHTNHQQTEFNNDALKFQALEELPSQIKSYVSNFEIKDIR
                     IIKSILLKGKKSFNNTHDTYYRLEDVEFELVSVLKRFKAMLLQKNETVETMQGYLMQSI
                     KAELEEIHALNMRRQNIPQYNIFNQ"
     gene            2535..3455
                     /gene="rep"
                     /label="rep"
     promoter        3551..3655
                     /label="AmpR promoter"
     CDS             3656..4513
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4687..5275
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5461..5601)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(5749..6591)
                     /gene="blaZ"
                     /label="Beta-lactamase"
                     /note="Beta-lactamase from Staphylococcus aureus.
                     Accession#: P00807"
ORIGIN
        1 aagcttggct gcaggtcgac ggatccccgg gaattcctgg cagtttatgg cgggcgtcct
       61 gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc
      121 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
      181 tgagcctttc gttttatttg atgcctgggg aattcttgaa gacgaaaggg cctcgtgata
      241 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc ttcaactaaa
      301 gcacccatta gttcaacaaa cgaaaattgg ataaagtggg atatttttaa aatatatatt
      361 tatgttacag taatattgac ttttaaaaaa ggattgattc taatgaagaa agcagacaag
      421 taagcctcct aaattcactt tagataaaaa tttaggaggc atatcaaatg aactttaata
      481 aaattgattt agacaattgg aagagaaaag agatatttaa tcattatttg aaccaacaaa
      541 cgacttttag tataaccaca gaaattgata ttagtgtttt ataccgaaac ataaaacaag
      601 aaggatataa attttaccct gcatttattt tcttagtgac aagggtgata aactcaaata
      661 cagcttttag aactggttac aatagcgacg gagagttagg ttattgggat aagttagagc
      721 cactttatac aatttttgat ggtgtatcta aaacattctc tggtatttgg actcctgtaa
      781 agaatgactt caaagagttt tatgatttat acctttctga tgtagagaaa tataatggtt
      841 cggggaaatt gtttcccaaa acacctatac ctgaaaatgc tttttctctt tctattattc
      901 catggacttc atttactggg tttaacttaa atatcaataa taatagtaat taccttctac
      961 ccattattac agcaggaaaa ttcattaata aaggtaattc aatatattta ccgctatctt
     1021 tacaggtaca tcattctgtt tgtgatggtt atcatgcagg attgtttatg aactctattc
     1081 aggaattgtc agataggcct aatgactggc ttttataata tgagataatg ccgactgtac
     1141 tttttacagt cggttttcta atgtcactaa cctgccccgt tagttgaaga aggtttttat
     1201 attacagctc cagatccata tccttcagat ccctagataa ttcttctgat aatttagttt
     1261 ttgttagagt ttaacataac tgatgattat cagaagtttc tttaatataa ataaaagcac
     1321 tataagcgac ttaacttata gtgcttattt aatatatttt ttcaaacaat cattagccac
     1381 caaacaaacg actccagaag ccttttttag tttgggtttc ttctatagca atatctttat
     1441 cattttgatt aaatgaatca tcttgttgtt gtgatttttc atcttgttga ctctcagata
     1501 tatctttagg ttgaacctct ggtttctttt cttctttctg atattgatca gtatttacag
     1561 aatccgaagt atatgatgct tcttgtacat caaagttttc tcgatcatta gtagatttat
     1621 caaaggaata acttaattgt ctctcttcct ctaattgatg ttccaatttt tgaatttttt
     1681 tattgctttc taaagctaaa atttgttgat tctctaataa tttatttaat gtattaacat
     1741 tgctattttg ttggtctagt tgtttagtta aattctcaat atacttttca tcatttttag
     1801 ctctagtttc aaagatttct acctgctttt ctaattcgtt tacttttgtt tttaatgtct
     1861 caaagccatc tgaattatta tatctattct tttcagtgtt ttcataagtt atttctgatt
     1921 ttttattttc tttattttga gttgttcttt ctttcttagt gattttttct actattttca
     1981 aataatcatt atcatcaata taattcactc cattttcttt ttcaaaagat atattcaaat
     2041 ttttagcatt attaacaaca gtttgtttag ttacattcaa ttcatcagca accattttta
     2101 tagttttcat atcatccacc cttaggcacc taatttacta cctaatttta ccacctaaca
     2161 atttttcttt catctttttt accgcctaaa tatatcattt ttactaccta agtacctaat
     2221 ttactaccta attagccact aaaataataa attttgttgt tttaaggtca taacatgatt
     2281 ctgatctgta cttaaaacgc tttatataca cctttaaaaa tgttaatatt atcttatgaa
     2341 ttttaaaaag ccaatgctat tggcgtagca tcggctctgg taattaaaac gatttgcgtt
     2401 cgtttattta tatatttttt tgatacttgt attatatata tctactcgtc taagtgcaag
     2461 cacaaaacat ataacttacg taaaaattgt tttattacct caactctaag taaaaggaaa
     2521 tgaggttttt tattatgtct aaatttaaaa aaatatctgc aagtgaattc gaaacattac
     2581 gtttttatca attacccaaa tttttatttg aagatgaata cttttctaaa atgcccacag
     2641 atgcaaaagt tatgtatgct ttattaaaag atcgctttga actatcaaga ttaaataact
     2701 gggtagattc agaaaataat atttatcttt tatataccaa taaacagtta tgctcaattt
     2761 taaattatgc agaaccaaaa atcattaaat taaaaaaaga attagaaaaa tacaatttga
     2821 ttataaacga aagacaaggt ttaaataaac ctaacaaaat ttatttactc gaacccacat
     2881 atgacaagga actaataaat tctaagttcc agaacaaaga atttattagt tccagaacta
     2941 ataaatcatc agttcaagaa ctaataaatt ctaagtcaag tgatactgat tttaataata
     3001 ctgaatatat agagactaag aataatgata cgaattatac gaatgataca tctaacatga
     3061 tttctaagaa ttctcattcg aatcatacaa atcatcaaca aaccgaattt aataatgatg
     3121 ccttaaaatt ccaggcgctt gaagaattac cttcgcaaat caaatcttat gtaagtaatt
     3181 ttgaaattaa agacatccgt attattaaaa gtatcttact caaggggaaa aagtcattta
     3241 ataatacaca tgatacatat taccgtttag aagacgtcga atttgaactt gtaagtgttt
     3301 taaaacgttt taaagccatg ttgctacaaa aaaatgaaac cgttgaaact atgcaaggct
     3361 atttaatgca atcaattaaa gctgaacttg aagaaataca tgcattaaat atgcgtcgtc
     3421 aaaacatacc tcaatacaat atctttaatc aataactcaa ataatcttac aacaatcaaa
     3481 acaacatcaa aatttggaat taagtcaaca gaaaaggatc tccccaggtg gcacttttcg
     3541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3601 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3661 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3721 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3781 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3841 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     3901 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3961 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4021 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4081 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4141 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     4201 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4261 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4321 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4381 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4441 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4501 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4561 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4621 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4681 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4741 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4801 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4861 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4921 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4981 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5041 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     5101 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5161 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5221 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5281 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5341 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     5401 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     5461 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     5521 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     5581 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     5641 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5701 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc aggggttaaa attccttcat
     5761 tacactcttg gcggtttcac ttatcaactt atcatttggc ttatcacttt tattgtcttt
     5821 attcgtaaaa atgactaaaa caataggttc agattggccc ttaggataaa caaaagcaac
     5881 atcatttcta gaagcatatg ttattgcttg accactttta tcagcaacct tatagtcttt
     5941 tggaacaccg tctttaatta aagtatctcc gcttttatta tttaacatta aatcaagtaa
     6001 gaatttcttg ttttctttgc ttaattttcc atttgcgata agtttattta aagtcttacc
     6061 gaaagcagca ggtgttgaag tatctttttt gctctttggt gaatagtaat ttaattctat
     6121 ctcatatcta actggatttg ttactttatc tcctagttct tttagacgtt gtttaacttt
     6181 tttgattcca ccgatttctt ttataatttt attgtttgct gtattatcac tatatgtcat
     6241 tgaagcctca ataagtgctt ttaaagtgat atcttttcct acatattttt ctaaaatagg
     6301 agaataagca actatatcat ctttgttaat atgtactttt ttatttaact tattataagg
     6361 tacttgttct aacaaaatag cactatttat cgcttttgaa gttgaagcat aggcaaatct
     6421 cttatctgaa ttaaatttta cttccttacc acttttagta tctaaagcat aaacaccaat
     6481 atgagcatta tatttttttt ctaaatcatt taactctttg gcatgtgaac tgtttgaatt
     6541 acatgcactt aaaactaaag caattacaat taaaaatatt aactttttca taataaaccc
     6601 tccgattacc tacct
//
