LOCUS       V003157                 6152 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003157
VERSION     V003157
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Stable low-copy-number Staphylococcus aureus shuttle vectors
  JOURNAL   Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)
   PUBMED   12634346
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2002) Biological Sciences, University of Sydney,
            Macleay Building, A12, Sydney, NSW 2006, Australia
REFERENCE   3  (bases 1 to 6152)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-NOV-2002) Biological Sciences, University of Sydney, Macleay
            Building, A12, Sydney, NSW 2006, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(58..74)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             700..1347
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1605..2342)
                     /codon_start=1
                     /gene="par"
                     /product="partitioning protein"
                     /label="par"
                     /note="Par"
                     /protein_id="AAO24790.1"
                     /translation="MKTIKMVADELNVTKQTVVNNAKNLNISFEKENGVNYIDDNDYLK
                     IVEKITKKERTTQNKENKKSEITYENTEKNRYNNSDGFETLKTKVNELEKQVEIFETRA
                     KNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLEHQLEEERQLSYSF
                     DKSTNDRENFDVQEASYTSDSVNTDQYQKEEKKPEVQPKDISESQQDEKSQQQDDSFNQ
                     NDKDIAIEETQTKKGFWSRLFGG"
     gene            complement(1605..2342)
                     /gene="par"
                     /label="par"
     CDS             2767..3687
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label="rep"
                     /note="Rep"
                     /protein_id="AAO24791.1"
                     /translation="MSKFKKISASEFETLRFYQLPKFLFEDEYFSKMPTDAKVMYALLK
                     DRFELSRLNNWVDSENNIYLLYTNKQLCSILNYAEPKIIKLKKELEKYNLIINERQGLN
                     KPNKIYLLEPTYDKELINSKFQNKEFISSRTNKSSVQELINSKSSDTDFNNTEYIETKN
                     NDTNYTNDTSNMISKNSHSNHTNHQQTEFNNDALKFQALEELPSQIKSYVSNFEIKDIR
                     IIKSILLKGKKSFNNTHDTYYRLEDVEFELVSVLKRFKAMLLQKNETVETMQGYLMQSI
                     KAELEEIHALNMRRQNIPQYNIFNQ"
     gene            2767..3687
                     /gene="rep"
                     /label="rep"
     promoter        3783..3887
                     /label="AmpR promoter"
     CDS             3888..4745
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4919..5507
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5693..5833)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     protein_bind    6026..6047
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6062..6092
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6100..6116
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6124..6140
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
       61 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      121 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      181 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
      241 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      301 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
      361 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      421 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
      481 tttataggtt aatgtcatga taataatggt ttcttagacg tcttcaacta aagcacccat
      541 tagttcaaca aacgaaaatt ggataaagtg ggatattttt aaaatatata tttatgttac
      601 agtaatattg acttttaaaa aaggattgat tctaatgaag aaagcagaca agtaagcctc
      661 ctaaattcac tttagataaa aatttaggag gcatatcaaa tgaactttaa taaaattgat
      721 ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt
      781 agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat
      841 aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt
      901 agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga gccactttat
      961 acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac
     1021 ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa
     1081 ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat tccatggact
     1141 tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct acccattatt
     1201 acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta
     1261 catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg
     1321 tcagataggc ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca
     1381 gtcggttttc taatgtcact aacctgcccc gttagttgaa gaaggttttt atattacagc
     1441 tccagatcca tatccttcag atccctagat aattcttctg ataatttagt ttttgttaga
     1501 gtttaacata actgatgatt atcagaagtt tctttaatat aaataaaagc actataagcg
     1561 acttaactta tagtgcttat ttaatatatt ttttcaaaca atcattagcc accaaacaaa
     1621 cgactccaga agcctttttt agtttgggtt tcttctatag caatatcttt atcattttga
     1681 ttaaatgaat catcttgttg ttgtgatttt tcatcttgtt gactctcaga tatatcttta
     1741 ggttgaacct ctggtttctt ttcttctttc tgatattgat cagtatttac agaatccgaa
     1801 gtatatgatg cttcttgtac atcaaagttt tctcgatcat tagtagattt atcaaaggaa
     1861 taacttaatt gtctctcttc ctctaattga tgttccaatt tttgaatttt tttattgctt
     1921 tctaaagcta aaatttgttg attctctaat aatttattta atgtattaac attgctattt
     1981 tgttggtcta gttgtttagt taaattctca atatactttt catcattttt agctctagtt
     2041 tcaaagattt ctacctgctt ttctaattcg tttacttttg tttttaatgt ctcaaagcca
     2101 tctgaattat tatatctatt cttttcagtg ttttcataag ttatttctga ttttttattt
     2161 tctttatttt gagttgttct ttctttctta gtgatttttt ctactatttt caaataatca
     2221 ttatcatcaa tataattcac tccattttct ttttcaaaag atatattcaa atttttagca
     2281 ttattaacaa cagtttgttt agttacattc aattcatcag caaccatttt tatagttttc
     2341 atatcatcca cccttaggca cctaatttac tacctaattt taccacctaa caatttttct
     2401 ttcatctttt ttaccgccta aatatatcat ttttactacc taagtaccta atttactacc
     2461 taattagcca ctaaaataat aaattttgtt gttttaaggt cataacatga ttctgatctg
     2521 tacttaaaac gctttatata cacctttaaa aatgttaata ttatcttatg aattttaaaa
     2581 agccaatgct attggcgtag catcggctct ggtaattaaa acgatttgcg ttcgtttatt
     2641 tatatatttt tttgatactt gtattatata tatctactcg tctaagtgca agcacaaaac
     2701 atataactta cgtaaaaatt gttttattac ctcaactcta agtaaaagga aatgaggttt
     2761 tttattatgt ctaaatttaa aaaaatatct gcaagtgaat tcgaaacatt acgtttttat
     2821 caattaccca aatttttatt tgaagatgaa tacttttcta aaatgcccac agatgcaaaa
     2881 gttatgtatg ctttattaaa agatcgcttt gaactatcaa gattaaataa ctgggtagat
     2941 tcagaaaata atatttatct tttatatacc aataaacagt tatgctcaat tttaaattat
     3001 gcagaaccaa aaatcattaa attaaaaaaa gaattagaaa aatacaattt gattataaac
     3061 gaaagacaag gtttaaataa acctaacaaa atttatttac tcgaacccac atatgacaag
     3121 gaactaataa attctaagtt ccagaacaaa gaatttatta gttccagaac taataaatca
     3181 tcagttcaag aactaataaa ttctaagtca agtgatactg attttaataa tactgaatat
     3241 atagagacta agaataatga tacgaattat acgaatgata catctaacat gatttctaag
     3301 aattctcatt cgaatcatac aaatcatcaa caaaccgaat ttaataatga tgccttaaaa
     3361 ttccaggcgc ttgaagaatt accttcgcaa atcaaatctt atgtaagtaa ttttgaaatt
     3421 aaagacatcc gtattattaa aagtatctta ctcaagggga aaaagtcatt taataataca
     3481 catgatacat attaccgttt agaagacgtc gaatttgaac ttgtaagtgt tttaaaacgt
     3541 tttaaagcca tgttgctaca aaaaaatgaa accgttgaaa ctatgcaagg ctatttaatg
     3601 caatcaatta aagctgaact tgaagaaata catgcattaa atatgcgtcg tcaaaacata
     3661 cctcaataca atatctttaa tcaataactc aaataatctt acaacaatca aaacaacatc
     3721 aaaatttgga attaagtcaa cagaaaagga tctccccagg tggcactttt cggggaaatg
     3781 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3841 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3901 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3961 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4021 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4081 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     4141 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4201 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4261 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4321 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4381 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     4441 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4501 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4561 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4621 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4681 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4741 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4801 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4861 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4921 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4981 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5041 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     5101 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5161 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5221 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5281 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     5341 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5401 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5461 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5521 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5581 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5641 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     5701 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     5761 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     5821 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5881 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5941 gttttcaccg tcatcaccga aacgcgcgag gcagctggca cgacaggttt cccgactgga
     6001 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6061 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6121 acacaggaaa cagctatgac catgattacg cc
//
