LOCUS       V003159                 5912 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003159
VERSION     V003159
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Stable low-copy-number Staphylococcus aureus shuttle vectors
  JOURNAL   Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)
   PUBMED   12634346
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Grkovic S, Brown MH, Hardie KM, Firth N, Skurray RA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2002) Biological Sciences, University of Sydney,
            Macleay Building, A12, Sydney, NSW 2006, Australia
REFERENCE   3  (bases 1 to 5912)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 149 (PT 3), 785-794 (2003)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-NOV-2002) Biological Sciences, University of Sydney, Macleay
            Building, A12, Sydney, NSW 2006, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..31
                     /note="multiple cloning site (MCS); contains uniques sites
                     for HindIII, SalI, BamHI and SmaI"
     terminator      complement(115..201)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             468..1115
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1373..2110)
                     /codon_start=1
                     /gene="par"
                     /product="partitioning protein"
                     /label="par"
                     /note="Par"
                     /protein_id="AAO24782.1"
                     /translation="MKTIKMVADELNVTKQTVVNNAKNLNISFEKENGVNYIDDNDYLK
                     IVEKITKKERTTQNKENKKSEITYENTEKNRYNNSDGFETLKTKVNELEKQVEIFETRA
                     KNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLEHQLEEERQLSYSF
                     DKSTNDRENFDVQEASYTSDSVNTDQYQKEEKKPEVQPKDISESQQDEKSQQQDDSFNQ
                     NDKDIAIEETQTKKGFWSRLFGG"
     gene            complement(1373..2110)
                     /gene="par"
                     /label="par"
     CDS             2535..3455
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label="rep"
                     /note="Rep"
                     /protein_id="AAO24783.1"
                     /translation="MSKFKKISASEFETLRFYQLPKFLFEDEYFSKMPTDAKVMYALLK
                     DRFELSRLNNWVDSENNIYLLYTNKQLCSILNYAEPKIIKLKKELEKYNLIINERQGLN
                     KPNKIYLLEPTYDKELINSKFQXKEFISSRTNKSSVQELINSKSSDTDFNNTEYIETKN
                     NDTNYTNDTSNMISKNSHSNHTNHQQTEFNNDALKFQALEELPSQIKSYVSNFEIKDIR
                     IIKSILLKGKKSFNNTHDTYYRLEDVEFELVSVLKRFKAMLLQKNETVETMQGYLMQSI
                     KAELEEIHALNMRRQNIPQYNIFNQ"
     gene            2535..3455
                     /gene="rep"
                     /label="rep"
     promoter        3550..3654
                     /label="AmpR promoter"
     CDS             3655..4512
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4686..5274
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5460..5600)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 aagcttggct gcaggtcgac ggatccccgg gaattccctg gcagtttatg gcgggcgtcc
       61 tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt
      121 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      181 ctgagccttt cgttttattt gatgcctggg aattcttgaa gacgaaaggg cctcgtgata
      241 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc ttcaactaaa
      301 gcacccatta gttcaacaaa cgaaaattgg ataaagtggg atatttttaa aatatatatt
      361 tatgttacag taatattgac ttttaaaaaa ggattgattc taatgaagaa agcagacaag
      421 taagcctcct aaattcactt tagataaaaa tttaggaggc atatcaaatg aactttaata
      481 aaattgattt agacaattgg aagagaaaag agatatttaa tcattatttg aaccaacaaa
      541 cgacttttag tataaccaca gaaattgata ttagtgtttt ataccgaaac ataaaacaag
      601 aaggatataa attttaccct gcatttattt tcttagtgac aagggtgata aactcaaata
      661 cagcttttag aactggttac aatagcgacg gagagttagg ttattgggat aagttagagc
      721 cactttatac aatttttgat ggtgtatcta aaacattctc tggtatttgg actcctgtaa
      781 agaatgactt caaagagttt tatgatttat acctttctga tgtagagaaa tataatggtt
      841 cggggaaatt gtttcccaaa acacctatac ctgaaaatgc tttttctctt tctattattc
      901 catggacttc atttactggg tttaacttaa atatcaataa taatagtaat taccttctac
      961 ccattattac agcaggaaaa ttcattaata aaggtaattc aatatattta ccgctatctt
     1021 tacaggtaca tcattctgtt tgtgatggtt atcatgcagg attgtttatg aactctattc
     1081 aggaattgtc agataggcct aatgactggc ttttataata tgagataatg ccgactgtac
     1141 tttttacagt cggttttcta atgtcactaa cctgccccgt tagttgaaga aggtttttat
     1201 attacagctc cagatccata tccttcagat ccctagataa ttcttctgat aatttagttt
     1261 ttgttagagt ttaacataac tgatgattat cagaagtttc tttaatataa ataaaagcac
     1321 tataagcgac ttaacttata gtgcttattt aatatatttt ttcaaacaat cattagccac
     1381 caaacaaacg actccagaag ccttttttag tttgggtttc ttctatagca atatctttat
     1441 cattttgatt aaatgaatca tcttgttgtt gtgatttttc atcttgttga ctctcagata
     1501 tatctttagg ttgaacctct ggtttctttt cttctttctg atattgatca gtatttacag
     1561 aatccgaagt atatgatgct tcttgtacat caaagttttc tcgatcatta gtagatttat
     1621 caaaggaata acttaattgt ctctcttcct ctaattgatg ttccaatttt tgaatttttt
     1681 tattgctttc taaagctaaa atttgttgat tctctaataa tttatttaat gtattaacat
     1741 tgctattttg ttggtctagt tgtttagtta aattctcaat atacttttca tcatttttag
     1801 ctctagtttc aaagatttct acctgctttt ctaattcgtt tacttttgtt tttaatgtct
     1861 caaagccatc tgaattatta tatctattct tttcagtgtt ttcataagtt atttctgatt
     1921 ttttattttc tttattttga gttgttcttt ctttcttagt gattttttct actattttca
     1981 aataatcatt atcatcaata taattcactc cattttcttt ttcaaaagat atattcaaat
     2041 ttttagcatt attaacaaca gtttgtttag ttacattcaa ttcatcagca accattttta
     2101 tagttttcat atcatccacc cttaggcacc taatttacta cctaatttta ccacctaaca
     2161 atttttcttt catctttttt accgcctaaa tatatcattt ttactaccta agtacctaat
     2221 ttactaccta attagccact aaaataataa attttgttgt tttaaggtca taacatgatt
     2281 ctgatctgta cttaaaacgc tttatataca cctttaaaaa tgttaatatt atcttatgaa
     2341 ttttaaaaag ccaatgctat tggcgtagca tcggctctgg taattaaaac gatttgcgtt
     2401 cgtttattta tatatttttt tgatacttgt attatatata tctactcgtc taagtgcaag
     2461 cacaaaacat ataacttacg taaaaattgt tttattacct caactctaag taaaaggaaa
     2521 tgaggttttt tattatgtct aaatttaaaa aaatatctgc aagtgaattc gaaacattac
     2581 gtttttatca attacccaaa tttttatttg aagatgaata cttttctaaa atgcccacag
     2641 atgcaaaagt tatgtatgct ttattaaaag atcgctttga actatcaaga ttaaataact
     2701 gggtagattc agaaaataat atttatcttt tatataccaa taaacagtta tgctcaattt
     2761 taaattatgc agaaccaaaa atcattaaat taaaaaaaga attagaaaaa tacaatttga
     2821 ttataaacga aagacaaggt ttaaataaac ctaacaaaat ttatttactc gaacccacat
     2881 atgacaagga actaataaat tctaagttcc agamcaaaga atttattagt tccagaacta
     2941 ataaatcatc agttcaagaa ctaataaatt ctaagtcaag tgatactgat tttaataata
     3001 ctgaatatat agagactaag aataatgata cgaattatac gaatgataca tctaacatga
     3061 tttctaagaa ttctcattcg aatcatacaa atcatcaaca aaccgaattt aataatgatg
     3121 ccttaaaatt ccaggcgctt gaagaattac cttcgcaaat caaatcttat gtaagtaatt
     3181 ttgaaattaa agacatccgt attattaaaa gtatcttact caaggggaaa aagtcattta
     3241 ataatacaca tgatacatat taccgtttag aagacgtcga atttgaactt gtaagtgttt
     3301 taaaacgttt taaagccatg ttgctacaaa aaaatgaaac cgttgaaact atgcaaggct
     3361 atttaatgca atcaattaaa gctgaacttg aagaaataca tgcattaaat atgcgtcgtc
     3421 aaaacatacc tcaatacaat atctttaatc aataactcaa ataatcttac aacaatcaaa
     3481 acaacatcaa aatttggaat taagtcaaca gaaaaggatc tcccaggtgg cacttttcgg
     3541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     3901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     4201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4681 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4741 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4801 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4861 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4921 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4981 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5041 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     5101 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5161 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5221 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5281 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5341 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5401 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5461 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     5521 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     5581 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5641 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5701 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgaacggt ctggttatag
     5761 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcga ttgggatata
     5821 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     5881 ctcgtcgaag ctcggcggat ttgtcctact ca
//
