LOCUS       40924_40557        7664 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSK485, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Hartmann T, Dumig M, Jaber BM, Szewczyk E, Olbermann P, Morschhauser
            J, Krappmann S.
  TITLE     Validation of a Self-Excising Marker in the Human Pathogen 
            Aspergillus fumigatus by Employing the {beta}-Rec/six Site-Specific 
            Recombination System
  JOURNAL   Appl. Environ. Microbiol. 76 (18), 6313-6317 (2010)
  PUBMED    20656854
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Duemig M, Krappmann S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2010) YIG3, Research Center for Infectious 
            Diseases, Josef-Scneider-Str. 2/D15, Wuerzburg 97080, Germany
REFERENCE   3  (bases 1 to 7664)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2010"; volume: "76"; issue: "18"; 
            pages: "6313-6317"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUL-2010) YIG3, Research Center for Infectious Diseases, 
            Josef-Scneider-Str. 2/D15, Wuerzburg 97080, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_recomb     389..478
                     /label=six site
                     /note="six site"
     regulatory      484..2171
                     /note="derived from Penicillium chrysogenum xylP; similar
                     to INSD accession M98458.2"
                     /regulatory_class="promoter"
     regulatory      2028..2032
                     /regulatory_class="CAAT_signal"
     regulatory      2095..2099
                     /regulatory_class="TATA_box"
     CDS             2177..2794
                     /codon_start=1
                     /gene="beta"
                     /product="beta-recombinase"
                     /label=beta
                     /note="beta-rec; codon optimized for expression in
                     Aspergillus fumigatus"
                     /protein_id="ADM43596.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQLQ
                     AMLNYIREGDIVVVTELDRLGRNNKELTELMNAIQQKGATLEVLNLPSMNGIEDENLRR
                     LINNLVIELYKYQAESERKRIKERQAQGIEIAKSKGKFKGRQHKFKENDPRLKHAFDLF
                     LNGCSDKEVEEQTGINRRTFRRYRTRYNVTVDQRKNKGKRDS"
     gene            2177..2794
                     /gene="beta"
                     /label=beta
     terminator      3010..3573
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
     gene            complement(3573..5560)
                     /label=ptrA
                     /note="useful as a dominant selectable marker in
                     Aspergillus"
     misc_recomb     5583..5672
                     /label=six site
                     /note="six site"
     rep_origin      complement(5917..6505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6679..7536)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7537..7641)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgcatg gtaccggcca ctcaggccta taggtcaata gagtatactt atttgtccta
      421 ttgattagat agcagtataa tagctttata gagtaggtca tttaagttga gcataatagt
      481 taactgcagg cggccgccga cggaagcgcg cagtcggcga agacagctgg cactgatgcg
      541 agcaacagta tgccagatga cgccaggcca gtgtttgact tgaagaatct cattgtcgtc
      601 aagattgtag tgtgtgatgg tttatttgat tgaagacttg gagcaggggc tagcctcgct
      661 taaatacttc aagtacggct tgtaagtcga attgtttggc ccggccgaat gaatcaaact
      721 aattcctcat tatcgacatt aaaatccaca atgtttcaat cggaagaaat gaccccttgg
      781 tatgtttttg atttgctgcc ttcaaaggga tcgtccagga taaactacat tagccagaag
      841 ccttagtccg ccggactatt gcctttctag caacaggtac aggcatggac tacgcatcaa
      901 cttaaccatt ccaagcctat tttcagacgg aggctgggta aattcatcac agaggtcatg
      961 aatctagccg tgggtcttga tggtgacacc agacggcttg attcctcacc gacgcctggt
     1021 cattataccg ttgtttcaca aaataactca ctagtctaaa cggtgcttgc tatcttgtgt
     1081 ggcttgggcc ttgccgaaag ctctttgaag aacgtcagac gtgactctgc tcgggagtgc
     1141 ctcggaggat ttagcgggga aatcgagaaa aaggctgggc ttatttgaaa ccgaggcaag
     1201 ccctacttgg cgaaggagga gctctcgcta tttcggatga cccccgaggt ataactcata
     1261 gatgtctcga gggccccctc ttcgtttcaa ctaaacagat tattctaccc ttaagtataa
     1321 gccgacaact ttgtgggtcc ttgttacata tcggagcgag aactcataat aggcctagcc
     1381 tatatatcta aaacgacttt ggtgatctga taggattcag actaagttac tactccacat
     1441 atctttcaga ctaaagtctt ctataggagc atttaggcta aaaaaatgat ggaacattat
     1501 ttcatcctaa atatttacta ggatcttgga taaaataggc aagcatccgt tccccttgag
     1561 taaaagaaat gggccttgaa aaaagggagg aaaaaaaaaa agaaaaaaac tgtttgaaga
     1621 atgtcgagat acactcctgc tcgggggtac ctcggaagtt ggatattcga gaacgagacc
     1681 ctgttggagg tgatagacgt catctccaca actaggaccc ctttttcgtt ctaactacac
     1741 gggctgttct agccttaatc aaagctaaga gttgtgggcc ccttgttaca gctggaactg
     1801 tgtaataggt ccatatacct gaaaggaggc tggcggtttg atactattca aagtgcaagt
     1861 tactacgtgc cccacatgca ctccagaata aagtcctctc tgcgagaatt taggctaaag
     1921 aaagatcgac tataatttca tccgagctat ttgttaggat tttggataaa atatgtaaat
     1981 tcgttctcca ttcgtgaacg agatagatga tgtagccccc gttttgtatt ggggaaggat
     2041 ttccctgtct caagtatgaa cttcttaaga catgccatgc tggcttctgt tctgtataag
     2101 tatcgcctcc atccctcccc gacgatgtct tctatcacac tcattttcat tcatcgactc
     2161 gaagaaccaa ctgcagatgg ccaagatcgg ctacgcccgc gtcagctcca aggaacagaa
     2221 cctggaccgc caactccagg ccctccaggg cgtcagcaag gtgttctccg acaagctgtc
     2281 cggccagtcc gtcgagcgcc cccagctcca ggccatgctg aactacatcc gcgagggcga
     2341 catcgtcgtc gtcaccgagc tggaccgcct gggccgcaac aacaaggaac tgaccgagct
     2401 gatgaacgcc atccagcaga agggcgccac cctcgaggtc ctgaacctgc cctccatgaa
     2461 cggcatcgag gacgagaacc tgcgccgcct gatcaacaac ctggtcatcg agctgtacaa
     2521 gtaccaggcc gagtccgagc gcaagcgcat caaggaacgc caggcccagg gcatcgagat
     2581 cgccaagtcc aagggcaagt tcaagggccg ccagcacaag ttcaaggaaa acgacccccg
     2641 cctgaagcac gccttcgacc tgttcctcaa cggctgctcc gacaaggaag tcgaggaaca
     2701 gaccggcatc aaccgccgca ccttccgccg ctaccgcacc cgctacaacg tcaccgtcga
     2761 ccagcgcaag aacaagggca agcgcgactc ctgaggatcc ccgacgccga ccaacaccgc
     2821 cggtccgacg gcggcccacg ggtcccagga gcttgagatc cacttaacgt tactgaaatc
     2881 atcaaacagc ttgacgaatc tggatataag atcgttggtg tcgatgtcag ctccggagtt
     2941 gagacaaatg gtgttcagga tctcgataag atacgttcat ttgtccaagc agcaaagagt
     3001 gccttctagt gatttaatag ctccatgtca acaagaataa aacgcgtttt cgggtttacc
     3061 tcttccagat acagctcatc tgcaatgcat taatgcattg actgcaacct agtaacgcct
     3121 tcaggctccg gcgaagagaa gaatagctta gcagagctat tttcattttc gggagacgag
     3181 atcaagcaga tcaacggtcg tcaagagacc tacgagactg aggaatccgc tcttggctcc
     3241 acgcgactat atatttgtct ctaattgtac tttgacatgc tcctcttctt tactctgata
     3301 gcttgactat gaaaattccg tcaccagccc ctgggttcgc aaagataatt gcatgtttct
     3361 tccttgaact ctcaagccta caggacacac attcatcgta ggtataaacc tcgaaatcat
     3421 tcctactaag atggtataca atagtaacca tgcatggttg cctagtgaat gctccgtaac
     3481 acccaatacg ccggccgaaa cttttttaca actctcctat gagtcgttta cccagaatgc
     3541 acaggtacac ttgtttagag gtaatccttc tttcttgcat ctttgtttgt attatactgt
     3601 ctttcttgtt acacataatt attctagaat gccccaccgt tacatacggg acacagccat
     3661 ttacatatgc atgtggatta cgagctaacg agttcattca aatctcagaa ctatcacata
     3721 atcatcattc ccctatcgtc aaagaccgta agacaaatcc ggttcatgca ctgaacccat
     3781 tcgggtagtg agtcatttac tcagcacact cgcgctgacg ctcgtcgaac accttcaatg
     3841 cctcctcggc agccttgaca ccactgagaa ccatggcacc gaaggtaggg cccatgcggt
     3901 taaagccatc aatttcagac agctccatac cgccgattat caagccctta gtaacctcgc
     3961 gggtgttctt gacgatggca tcctcggccg agttcatgtc gagaccacgc atgccaccta
     4021 gcttgtcgac gctgcccatg gacaccaagc gcttcgcaca gaaggcgccg aatggcccat
     4081 cgtgaccagt ggtactgatg atgacaggag cgttgatagt gttggggtcc atgcaggagt
     4141 gatcatcgtg gtgaagggtg accagcgtcc agttgacgac aacaccagca atctgggggt
     4201 tgccgttctc ggtcggacgg gtgatcaagt cctcaacagc ggtagcattg aagagcttga
     4261 cattggggaa ggagagaacc ttcgacatga gtgtcgaggt aaacagggag gcgtgcttga
     4321 cgacaacgta gttggggttt gcgtcctctt cgtaaggaac acccagctcg ttcaggaaga
     4381 cttccgcggg acggcgcatg accatagcag aaaagagttg gccacccaac caggcaccgc
     4441 cacctattgc acgttagttc cggaaagctg agtgcaaggc aatccatcat ggactactga
     4501 ccaggagaga cgctggcctc gacgatagca atcttcaggt ccggacgagc cttggccaag
     4561 acgtacgcag tgctcagacc gcaggaacca gcaccaacaa tgacaacgtc actttcagcg
     4621 tacttgtcca ggtcctcaaa gtaacgtctg gtcatggcac gagagacctg gctttcgcgg
     4681 ataggggcga acttgaactc gtcccacttg ccaccgaaat ggtccaacag cttggtctga
     4741 gaagctccct caacggggac ggtctcagaa accacgacct tacccttgag gccggtagcg
     4801 gccacagtgg gttcgtagat ggcagctgga ggagacatgt ttcaagttgc aatgactatc
     4861 atctgttagc cattccatca acaggaagaa cgagagaagg catgaccctt ttcgctggta
     4921 ttatccagat caagttttag ccgtataatc tcagaacgaa cccagtccat cgatgccatg
     4981 tccttctaga ctaggatcct agagtctagg gcccagctta gggagggcat gtgaatgcat
     5041 cgatgactgg gaacgaacac cggcccacgc caaagacgtt acctaagata ccttgatcat
     5101 tgtgagagtc cagccaaaag tattccatga cttccatcgt atgccctcta gagggctaat
     5161 cgaggagtgt atttacattg tcggttggtt tgggaactat agaagatggt cagttattcc
     5221 aatcaccaaa ggtttatcga agggaggaag acttgttcag tttcgtccga ggacttttgg
     5281 aattcaaatc tgagatagag aattgtgtgg gatgagagga aaaggaagaa gatggtgggg
     5341 ttcagaagga ggggttgagt taaatagcat gggttgagtc aacgtgataa gggcactata
     5401 ccgtatagat cagcggcacc cgattctatc cgttcctttt gctcctcttt agctttgacc
     5461 ggtgagccgg acaagaaaca agtgaaatca tcctgacatc ggcggacgat ctcctagctt
     5521 ttacatttcg ttaccaatgg gatcccgtaa tcaattgccc gtctgtcaga tcttctgata
     5581 tctataggtc aatagagtat acttatttgt cctattgatt agatagcagt ataatagctt
     5641 tatagagtag gtcatttaag ttgagcataa tatggccatc taggccgagc tcctgggcct
     5701 catgggcctt ccgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5761 aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg ctcactgact
     5821 cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa
     5881 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5941 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6001 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6061 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6121 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6181 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6241 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6301 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6361 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6421 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6481 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6541 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6601 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6661 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6721 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6781 cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga
     6841 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6901 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6961 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7021 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7081 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7141 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7201 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7261 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7321 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7381 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7441 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7501 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7561 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7621 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
