LOCUS       V003161                12416 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003161
VERSION     V003161
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
            Rozwadowski K.
  TITLE     TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
            expression vectors for functional analysis of proteins
  JOURNAL   Sci Rep 2, 874 (2012)
   PUBMED   23166857
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   Rozwadowski K, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
            0X2, Canada
REFERENCE   3  (bases 1 to 12416)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
            874 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..754
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    766..821
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             823..4173
                     /codon_start=1
                     /gene="Rep"
                     /product="replicase"
                     /label="Rep"
                     /protein_id="AFX83512.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     gene            823..4173
                     /gene="Rep"
                     /label="Rep"
     CDS             4249..5667
                     /codon_start=1
                     /gene="RNA pol"
                     /product="RNA polymerase"
                     /label="RNA pol"
                     /protein_id="AFX83513.1"
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
                     IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
                     TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
                     FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
                     KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
                     KVLFRSLFIDGSSC"
     gene            4249..5667
                     /gene="RNA pol"
                     /label="RNA pol"
     CDS             5651..6454
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     CDS             6517..6546
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             6553..6582
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             6589..6618
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     protein_bind    6622..6741
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6771..6801
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6855..7511
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7856..8158
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8202..8326)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8567..8611
                     /note="TobRV/G12a_Ribozyme: aligns with Tobacco ringspot
                     virus satellite RNA and Hammerhead ribozyme G12a"
     misc_feature    8654..8864
                     /label="CaMV 35S"
                     /note="CaMV 35S"
     misc_feature    8934..8958
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9113..10258)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10560..11351)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(11563..12198)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    12276..12300
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     12381..12397
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
       61 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      121 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      181 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      241 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      301 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact
      361 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
      421 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
      481 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      541 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
      601 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
      661 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      721 ctctatataa ggaagttcat ttcatttgga gagggtattt ttacaacaat taccaacaac
      781 aacaaacaac agacaacatt acaattacta tttacaatta caatggcata cacacagaca
      841 gctaccacat cagctttgct ggacactgtc cgaggaaaca actccttggt caatgatcta
      901 gcaaagcgtc gtctttacga cacagcggtt gaagagttta acgctcgtga ccgcaggccc
      961 aaggtgaact tttcaaaagt aataagcgag gagcagacgc ttattgctac ccgggcgtat
     1021 ccagaattcc aaattacatt ttataacacg caaaatgccg tgcattcgct tgcaggtgga
     1081 ttgcgatctt tagaactgga atatctgatg atgcaaattc cctacggatc attgacttat
     1141 gacataggcg ggaattttgc atcgcatctg ttcaagggac gagcatatgt acactgctgc
     1201 atgcccaacc tggacgttcg agacatcatg cggcacgaag gccagaaaga cagtattgaa
     1261 ctataccttt ctaggctaga gagagggggg aaaacagtcc ccaacttcca aaaggaagca
     1321 tttgacagat acgcagaaat tcctgaagac gctgtctgtc acaatacttt ccagacatgc
     1381 gaacatcagc cgatgcagca atcaggcaga gtgtatgcca ttgcgctaca cagcatatat
     1441 gacataccag ccgatgagtt cggggcggca ctcttgagga aaaatgtcca tacgtgctat
     1501 gccgctttcc acttctccga gaacctgctt cttgaagatt catgcgtcaa tttggacgaa
     1561 atcaacgcgt gtttttcgcg cgatggagac aagttgacct tttcttttgc atcagagagt
     1621 actcttaatt actgtcatag ttattctaat attcttaagt atgtgtgcaa aacttacttc
     1681 ccggcctcta atagagaggt ttacatgaag gagtttttag tcaccagagt taatacctgg
     1741 ttttgtaagt tttctagaat agatactttt cttttgtaca aaggtgtggc ccataaaagt
     1801 gtagatagtg agcagtttta tactgcaatg gaagacgcat ggcattacaa aaagactctt
     1861 gcaatgtgca acagcgagag aatcctcctt gaggattcat catcagtcaa ttactggttt
     1921 cccaaaatga gggatatggt catcgtacca ttattcgaca tttctttgga gactagtaag
     1981 aggacgcgca aggaagtctt agtgtccaag gatttcgtgt tcacagtgct taaccacatt
     2041 cgaacatacc aggcgaaagc tcttacatac gcaaatgttt tgtccttcgt cgaatcgatt
     2101 cgatcgaggg taatcattaa cggtgtgaca gcgaggtccg aatgggatgt ggacaaatct
     2161 ttgttacaat ccttgtccat gacgttttac ctgcatacta agcttgccgt tctaaaggat
     2221 gacttactga ttagcaagtt tagtctcggt tcgaaaacgg tgtgccagca tgtgtgggat
     2281 gagatttcgc tggcgtttgg gaacgcattt ccctccgtga aagagaggct cttgaacagg
     2341 aaacttatca gagtggcagg cgacgcatta gagatcaggg tgcctgatct atatgtgacc
     2401 ttccacgaca gattagtgac tgagtacaag gcctctgtgg acatgcctgc gcttgacatt
     2461 aggaagaaga tggaagaaac ggaagtgatg tacaatgcac tttcagaatt atcggtgtta
     2521 agggagtctg acaaattcga tgttgatgtt ttttcccaga tgtgccaatc tttggaagtt
     2581 gacccaatga cggcagcgaa ggttatagtc gcggtcatga gcaatgagag cggtctgact
     2641 ctcacatttg aacgacctac tgaggcgaat gttgcgctag ctttacagga tcaagagaag
     2701 gcttcagaag gtgcattggt agttacctca agagaagttg aagaaccgtc catgaagggt
     2761 tcgatggcca gaggagagtt acaattagct ggtcttgctg gagatcatcc ggaatcgtcc
     2821 tattctaaga acgaggagat agagtcttta gagcagtttc atatggcgac ggcagattcg
     2881 ttaattcgta agcagatgag ctcgattgtg tacacgggtc cgattaaagt tcagcaaatg
     2941 aaaaacttta tcgatagcct ggtagcatca ctatctgctg cggtgtcgaa tctcgtcaag
     3001 atcctcaaag atacagctgc tattgacctt gaaacccgtc aaaagtttgg agtcttggat
     3061 gttgcatcta ggaagtggtt aatcaaacca acggccaaga gtcatgcatg gggtgttgtt
     3121 gaaacccacg cgaggaagta tcatgtggcg cttttggaat atgatgagca gggtgtggtg
     3181 acatgcgatg attggagaag agtagctgtt agctctgagt ctgttgttta ttccgacatg
     3241 gcgaaactca gaactctgcg cagactgctt cgaaacggag aaccgcatgt cagtagcgca
     3301 aaggttgttc ttgtggacgg agttccgggc tgtggaaaaa ccaaagaaat tctttccagg
     3361 gttaattttg atgaagatct aattttagta cctgggaagc aagccgcgga aatgatcaga
     3421 agacgtgcga attcctcagg gattattgtg gccacgaagg acaacgttaa aaccgttgat
     3481 tctttcatga tgaattttgg gaaaagcaca cgctgtcagt tcaagaggtt attcattgat
     3541 gaagggttga tgttgcatac tggttgtgtt aattttcttg tggcgatgtc attgtgcgaa
     3601 attgcatatg tttacggaga cacacagcag attccataca tcaatagagt ttcaggattc
     3661 ccgtaccccg cccattttgc caaattggaa gttgacgagg tggagacacg cagaactact
     3721 ctccgttgtc cagccgatgt cacacattat ctgaacagga gatatgaggg ctttgtcatg
     3781 agcacttctt cggttaaaaa gtctgtttcg caggagatgg tcggcggagc cgccgtgatc
     3841 aatccgatct caaaaccctt gcatggcaag atcctgactt ttacccaatc ggataaagaa
     3901 gctctgcttt caagagggta ttcagatgtt cacactgtgc atgaagtgca aggcgagaca
     3961 tactctgatg tttcactagt taggttaacc cctacaccgg tctccatcat tgcaggagac
     4021 agcccacatg ttttggtcgc attgtcaagg cacacctgtt cgctcaagta ctacactgtt
     4081 gttatggatc ctttagttag tatcattaga gatctagaga aacttagctc gtacttgtta
     4141 gatatgtata aggtcgatgc aggaacacaa tagcaattac agattgactc ggtgttcaaa
     4201 ggttccaatc tttttgttgc agcgccaaag actggtgata tttctgatat gcagttttac
     4261 tatgataagt gtctcccagg caacagcacc atgatgaata attttgatgc tgttaccatg
     4321 aggttgactg acatttcatt gaatgtcaaa gattgcatat tggatatgtc taagtctgtt
     4381 gctgcgccta aggatcaaat caaaccacta atacctatgg tacgaacggc ggcagaaatg
     4441 ccacgccaga ctggactatt ggaaaattta gtggcgatga ttaaaagaaa ctttaacgca
     4501 cccgagttgt ctggcatcat tgatattgaa aatactgcat ctttggttgt agataagttt
     4561 tttgatagtt atttgcttaa agaaaaaaga aaaccaaata aaaatgtttc tttgttcagt
     4621 agagagtctc tcaatagatg gttagaaaag caggaacagg taacaatagg ccagctcgca
     4681 gattttgatt ttgtggattt gccagcagtt gatcagtaca gacacatgat taaagcacaa
     4741 cccaaacaaa agttggacac ttcaatccaa acggagtacc cggctttgca gacgattgtg
     4801 taccatcaaa aaagatcaat gcaatatcgc ccgttgttag tgagcttacc aggcaattac
     4861 tggacagtgt tgattcgagc agattttgtt ttcacaagaa agacaccagc gcagattgag
     4921 gatttcttcg gagatctcga cagtcatgtg ccgatggatg tcttggagct ggatatatca
     4981 aaatacgaca aatctcagaa tgaattccac tgtgcagtag aatacgagat ctggcgaaga
     5041 ttgggtttcg aagacttctt gggagaagtt tggaaacaag ggcatagaaa gaccaccctc
     5101 aaggattata ccgcaggtat aaaaacttgc atctggtatc aaagaaagag cggggacgtc
     5161 acgacgttca ttggaaacac tgtgatcatt gctgcatgtt tggcctcgat gcttccgatg
     5221 gagaaaataa tcaaaggagc cttttgcggt gacgatagtc tgctgtactt tccaaagggt
     5281 tgtgagtttc cggatgtgca acactccgcg aatcttatgt ggaattttga agcaaaactg
     5341 tttaaaaaac agtatggata cttttgcgga agatatgtaa tacatcacga cagaggatgc
     5401 attgtgtatt acgatcccct aaagttgatc tcgaaacttg gtgctaaaca catcaaggat
     5461 tgggaacact tggaggagtt cagaaggtct ctttgtgatg ttgctgtttc gttgaacaat
     5521 tgtgcgtatt acacacagtt ggacgacgct gtatgggagg ttcataagac cgcccctcca
     5581 ggttcgtttg tttataaaag tctggtgaag tatttgtctg ataaagttct ttttagaagt
     5641 ttgtttatag atggctctag ttgttaaagg aaaagtgaat atcaatgagt ttatcgacct
     5701 gacaaaaatg gagaagatct taccgtcgat gtttacccct gtaaagagtg ttatgtgttc
     5761 caaagttgat aaaataatgg ttcatgagaa tgagtcattg tcaggggtga accttcttaa
     5821 aggagttaag cttattgata gtggatacgt ctgtttagcc ggtttggtcg tcacgggcga
     5881 gtggaacttg cctgacaatt gcagaggagg tgtgagcgtg tgtctggtgg acaaaaggat
     5941 ggaaagagcc gacgaggcca ttctcggatc ttactacaca gcagctgcaa agaaaagatt
     6001 tcagttcaag gtcgttccca attatgctat aaccacccag gacgcgatga aaaacgtctg
     6061 gcaagtttta gttaatatta gaaatgtgaa gatgtcagcg ggtttctgtc cgctttctct
     6121 ggagtttgtg tcggtgtgta ttgtttatag aaataatata aaattaggtt tgagagagaa
     6181 gattacaaac gtgagagacg gagggcccat ggaacttaca gaagaagtcg ttgatgagtt
     6241 catggaagat gtccctatgt cgatcaggct tgcaaagttt cgatctcgaa ccggaaaaaa
     6301 gagtgatgtc cgcaaaggga aaaatagtag tagtgatcgg tcagtgccga acaagaacta
     6361 tagaaatgtt aaggattttg ggggaatgag ttttaaaaag aataatttaa tcgatgatga
     6421 ttcggaggct actgtcgccg aatcggattc gttttaaata gatcttacag tatcactact
     6481 ccatctcagt tcgtgttctt gtcattaatt aacatggaac aaaagctcat ttctgaagag
     6541 gatcttaatg gtgagcagaa attgatttca gaggaggatt tgaatggaga acagaaactg
     6601 atctctgaag aagatctgat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
     6661 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     6721 aaaacacaac atatccagtc atattggcgg ccgcattagg caccccaggc tttacacttt
     6781 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
     6841 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     6901 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     6961 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     7021 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
     7081 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     7141 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     7201 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     7261 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     7321 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     7381 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     7441 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     7501 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt
     7561 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     7621 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     7681 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     7741 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     7801 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     7861 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     7921 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     7981 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     8041 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     8101 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     8161 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     8221 gtgttttaca gcattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     8281 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgcct agggcggccg
     8341 cggtcctgca acttgaggta gtcaagatgc ataataaata acggattgtg tccgtaatca
     8401 cacgtggtgc gtacgataac gcatagtgtt tttccctcca cttaaatcga agggttgtgt
     8461 cttggatcgc gcgggtcaaa tgtatatggt tcatatacat ccgcaggcac gtaataaagc
     8521 gaggggttcg aatccccccg ttacccccgg taggggccca ggtacccgga tgtgttttcc
     8581 gggctgatga gtccgtgagg acgaaacctg gctgcaggca tgcaagcttg gcgtaatcat
     8641 ggtcatagcc tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta
     8701 tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg
     8761 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     8821 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca gcccggccgg
     8881 gggatccact agcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
     8941 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     9001 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acaggagatc
     9061 tcagtaaagc gctggctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc
     9121 aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt
     9181 cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca
     9241 tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga
     9301 acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt
     9361 ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt
     9421 tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa
     9481 cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct
     9541 tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga
     9601 aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca
     9661 gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga
     9721 cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga
     9781 acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa
     9841 tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg
     9901 cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg
     9961 tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt
    10021 cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt
    10081 gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga
    10141 aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg
    10201 aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc
    10261 accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg
    10321 acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt
    10381 agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca
    10441 gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa cacttgatgc
    10501 ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa
    10561 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
    10621 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
    10681 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
    10741 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
    10801 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
    10861 ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
    10921 tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
    10981 gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca
    11041 tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
    11101 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
    11161 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
    11221 tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct
    11281 tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc
    11341 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
    11401 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
    11461 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
    11521 cgattttgaa accacaatta tgggtgatgc tgccaactcg agagcgggcc gggagggttc
    11581 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt
    11641 taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc
    11701 cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa
    11761 atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg
    11821 cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca
    11881 ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg
    11941 aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg
    12001 cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca
    12061 agttttccgc gaggtatcca caacgccggc ggccggccgc ggtgtctcgc acacggcttc
    12121 gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca
    12181 accagcccgg tgagcgtcta gtggactgat gggctgcctg tatcgagtgg tgattttgtg
    12241 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
    12301 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggtggt
    12361 tttggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaatt
//
