LOCUS       V003163                12392 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003163
VERSION     V003163
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12392)
  AUTHORS   Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
            Rozwadowski K.
  TITLE     TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
            expression vectors for functional analysis of proteins
  JOURNAL   Sci Rep 2, 874 (2012)
   PUBMED   23166857
REFERENCE   2  (bases 1 to 12392)
  AUTHORS   Rozwadowski K, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
            0X2, Canada
REFERENCE   3  (bases 1 to 12392)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
            874 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..754
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    766..821
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             823..4173
                     /codon_start=1
                     /gene="Rep"
                     /product="replicase"
                     /label="Rep"
                     /protein_id="AFX83498.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     gene            823..4173
                     /gene="Rep"
                     /label="Rep"
     CDS             4249..5667
                     /codon_start=1
                     /gene="RNA pol"
                     /product="RNA polymerase"
                     /label="RNA pol"
                     /protein_id="AFX83499.1"
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
                     IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
                     TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
                     FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
                     KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
                     KVLFRSLFIDGSSC"
     gene            4249..5667
                     /gene="RNA pol"
                     /label="RNA pol"
     CDS             5651..6454
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     CDS             6517..6594
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags"
     protein_bind    6598..6717
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6747..6777
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6831..7487
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7832..8134
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8178..8302)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8543..8587
                     /note="TobRV/G12a_Ribozyme: aligns with Tobacco ringspot
                     virus satellite RNA and Hammerhead ribozyme G12a"
     misc_feature    8910..8934
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9089..10234)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10536..11327)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(11539..12174)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    12252..12276
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     12357..12373
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
       61 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      121 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      181 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      241 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      301 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact
      361 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
      421 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
      481 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      541 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
      601 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
      661 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      721 ctctatataa ggaagttcat ttcatttgga gagggtattt ttacaacaat taccaacaac
      781 aacaaacaac agacaacatt acaattacta tttacaatta caatggcata cacacagaca
      841 gctaccacat cagctttgct ggacactgtc cgaggaaaca actccttggt caatgatcta
      901 gcaaagcgtc gtctttacga cacagcggtt gaagagttta acgctcgtga ccgcaggccc
      961 aaggtgaact tttcaaaagt aataagcgag gagcagacgc ttattgctac ccgggcgtat
     1021 ccagaattcc aaattacatt ttataacacg caaaatgccg tgcattcgct tgcaggtgga
     1081 ttgcgatctt tagaactgga atatctgatg atgcaaattc cctacggatc attgacttat
     1141 gacataggcg ggaattttgc atcgcatctg ttcaagggac gagcatatgt acactgctgc
     1201 atgcccaacc tggacgttcg agacatcatg cggcacgaag gccagaaaga cagtattgaa
     1261 ctataccttt ctaggctaga gagagggggg aaaacagtcc ccaacttcca aaaggaagca
     1321 tttgacagat acgcagaaat tcctgaagac gctgtctgtc acaatacttt ccagacatgc
     1381 gaacatcagc cgatgcagca atcaggcaga gtgtatgcca ttgcgctaca cagcatatat
     1441 gacataccag ccgatgagtt cggggcggca ctcttgagga aaaatgtcca tacgtgctat
     1501 gccgctttcc acttctccga gaacctgctt cttgaagatt catgcgtcaa tttggacgaa
     1561 atcaacgcgt gtttttcgcg cgatggagac aagttgacct tttcttttgc atcagagagt
     1621 actcttaatt actgtcatag ttattctaat attcttaagt atgtgtgcaa aacttacttc
     1681 ccggcctcta atagagaggt ttacatgaag gagtttttag tcaccagagt taatacctgg
     1741 ttttgtaagt tttctagaat agatactttt cttttgtaca aaggtgtggc ccataaaagt
     1801 gtagatagtg agcagtttta tactgcaatg gaagacgcat ggcattacaa aaagactctt
     1861 gcaatgtgca acagcgagag aatcctcctt gaggattcat catcagtcaa ttactggttt
     1921 cccaaaatga gggatatggt catcgtacca ttattcgaca tttctttgga gactagtaag
     1981 aggacgcgca aggaagtctt agtgtccaag gatttcgtgt tcacagtgct taaccacatt
     2041 cgaacatacc aggcgaaagc tcttacatac gcaaatgttt tgtccttcgt cgaatcgatt
     2101 cgatcgaggg taatcattaa cggtgtgaca gcgaggtccg aatgggatgt ggacaaatct
     2161 ttgttacaat ccttgtccat gacgttttac ctgcatacta agcttgccgt tctaaaggat
     2221 gacttactga ttagcaagtt tagtctcggt tcgaaaacgg tgtgccagca tgtgtgggat
     2281 gagatttcgc tggcgtttgg gaacgcattt ccctccgtga aagagaggct cttgaacagg
     2341 aaacttatca gagtggcagg cgacgcatta gagatcaggg tgcctgatct atatgtgacc
     2401 ttccacgaca gattagtgac tgagtacaag gcctctgtgg acatgcctgc gcttgacatt
     2461 aggaagaaga tggaagaaac ggaagtgatg tacaatgcac tttcagaatt atcggtgtta
     2521 agggagtctg acaaattcga tgttgatgtt ttttcccaga tgtgccaatc tttggaagtt
     2581 gacccaatga cggcagcgaa ggttatagtc gcggtcatga gcaatgagag cggtctgact
     2641 ctcacatttg aacgacctac tgaggcgaat gttgcgctag ctttacagga tcaagagaag
     2701 gcttcagaag gtgcattggt agttacctca agagaagttg aagaaccgtc catgaagggt
     2761 tcgatggcca gaggagagtt acaattagct ggtcttgctg gagatcatcc ggaatcgtcc
     2821 tattctaaga acgaggagat agagtcttta gagcagtttc atatggcgac ggcagattcg
     2881 ttaattcgta agcagatgag ctcgattgtg tacacgggtc cgattaaagt tcagcaaatg
     2941 aaaaacttta tcgatagcct ggtagcatca ctatctgctg cggtgtcgaa tctcgtcaag
     3001 atcctcaaag atacagctgc tattgacctt gaaacccgtc aaaagtttgg agtcttggat
     3061 gttgcatcta ggaagtggtt aatcaaacca acggccaaga gtcatgcatg gggtgttgtt
     3121 gaaacccacg cgaggaagta tcatgtggcg cttttggaat atgatgagca gggtgtggtg
     3181 acatgcgatg attggagaag agtagctgtt agctctgagt ctgttgttta ttccgacatg
     3241 gcgaaactca gaactctgcg cagactgctt cgaaacggag aaccgcatgt cagtagcgca
     3301 aaggttgttc ttgtggacgg agttccgggc tgtggaaaaa ccaaagaaat tctttccagg
     3361 gttaattttg atgaagatct aattttagta cctgggaagc aagccgcgga aatgatcaga
     3421 agacgtgcga attcctcagg gattattgtg gccacgaagg acaacgttaa aaccgttgat
     3481 tctttcatga tgaattttgg gaaaagcaca cgctgtcagt tcaagaggtt attcattgat
     3541 gaagggttga tgttgcatac tggttgtgtt aattttcttg tggcgatgtc attgtgcgaa
     3601 attgcatatg tttacggaga cacacagcag attccataca tcaatagagt ttcaggattc
     3661 ccgtaccccg cccattttgc caaattggaa gttgacgagg tggagacacg cagaactact
     3721 ctccgttgtc cagccgatgt cacacattat ctgaacagga gatatgaggg ctttgtcatg
     3781 agcacttctt cggttaaaaa gtctgtttcg caggagatgg tcggcggagc cgccgtgatc
     3841 aatccgatct caaaaccctt gcatggcaag atcctgactt ttacccaatc ggataaagaa
     3901 gctctgcttt caagagggta ttcagatgtt cacactgtgc atgaagtgca aggcgagaca
     3961 tactctgatg tttcactagt taggttaacc cctacaccgg tctccatcat tgcaggagac
     4021 agcccacatg ttttggtcgc attgtcaagg cacacctgtt cgctcaagta ctacactgtt
     4081 gttatggatc ctttagttag tatcattaga gatctagaga aacttagctc gtacttgtta
     4141 gatatgtata aggtcgatgc aggaacacaa tagcaattac agattgactc ggtgttcaaa
     4201 ggttccaatc tttttgttgc agcgccaaag actggtgata tttctgatat gcagttttac
     4261 tatgataagt gtctcccagg caacagcacc atgatgaata attttgatgc tgttaccatg
     4321 aggttgactg acatttcatt gaatgtcaaa gattgcatat tggatatgtc taagtctgtt
     4381 gctgcgccta aggatcaaat caaaccacta atacctatgg tacgaacggc ggcagaaatg
     4441 ccacgccaga ctggactatt ggaaaattta gtggcgatga ttaaaagaaa ctttaacgca
     4501 cccgagttgt ctggcatcat tgatattgaa aatactgcat ctttggttgt agataagttt
     4561 tttgatagtt atttgcttaa agaaaaaaga aaaccaaata aaaatgtttc tttgttcagt
     4621 agagagtctc tcaatagatg gttagaaaag caggaacagg taacaatagg ccagctcgca
     4681 gattttgatt ttgtggattt gccagcagtt gatcagtaca gacacatgat taaagcacaa
     4741 cccaaacaaa agttggacac ttcaatccaa acggagtacc cggctttgca gacgattgtg
     4801 taccatcaaa aaagatcaat gcaatatcgc ccgttgttag tgagcttacc aggcaattac
     4861 tggacagtgt tgattcgagc agattttgtt ttcacaagaa agacaccagc gcagattgag
     4921 gatttcttcg gagatctcga cagtcatgtg ccgatggatg tcttggagct ggatatatca
     4981 aaatacgaca aatctcagaa tgaattccac tgtgcagtag aatacgagat ctggcgaaga
     5041 ttgggtttcg aagacttctt gggagaagtt tggaaacaag ggcatagaaa gaccaccctc
     5101 aaggattata ccgcaggtat aaaaacttgc atctggtatc aaagaaagag cggggacgtc
     5161 acgacgttca ttggaaacac tgtgatcatt gctgcatgtt tggcctcgat gcttccgatg
     5221 gagaaaataa tcaaaggagc cttttgcggt gacgatagtc tgctgtactt tccaaagggt
     5281 tgtgagtttc cggatgtgca acactccgcg aatcttatgt ggaattttga agcaaaactg
     5341 tttaaaaaac agtatggata cttttgcgga agatatgtaa tacatcacga cagaggatgc
     5401 attgtgtatt acgatcccct aaagttgatc tcgaaacttg gtgctaaaca catcaaggat
     5461 tgggaacact tggaggagtt cagaaggtct ctttgtgatg ttgctgtttc gttgaacaat
     5521 tgtgcgtatt acacacagtt ggacgacgct gtatgggagg ttcataagac cgcccctcca
     5581 ggttcgtttg tttataaaag tctggtgaag tatttgtctg ataaagttct ttttagaagt
     5641 ttgtttatag atggctctag ttgttaaagg aaaagtgaat atcaatgagt ttatcgacct
     5701 gacaaaaatg gagaagatct taccgtcgat gtttacccct gtaaagagtg ttatgtgttc
     5761 caaagttgat aaaataatgg ttcatgagaa tgagtcattg tcaggggtga accttcttaa
     5821 aggagttaag cttattgata gtggatacgt ctgtttagcc ggtttggtcg tcacgggcga
     5881 gtggaacttg cctgacaatt gcagaggagg tgtgagcgtg tgtctggtgg acaaaaggat
     5941 ggaaagagcc gacgaggcca ttctcggatc ttactacaca gcagctgcaa agaaaagatt
     6001 tcagttcaag gtcgttccca attatgctat aaccacccag gacgcgatga aaaacgtctg
     6061 gcaagtttta gttaatatta gaaatgtgaa gatgtcagcg ggtttctgtc cgctttctct
     6121 ggagtttgtg tcggtgtgta ttgtttatag aaataatata aaattaggtt tgagagagaa
     6181 gattacaaac gtgagagacg gagggcccat ggaacttaca gaagaagtcg ttgatgagtt
     6241 catggaagat gtccctatgt cgatcaggct tgcaaagttt cgatctcgaa ccggaaaaaa
     6301 gagtgatgtc cgcaaaggga aaaatagtag tagtgatcgg tcagtgccga acaagaacta
     6361 tagaaatgtt aaggattttg ggggaatgag ttttaaaaag aataatttaa tcgatgatga
     6421 ttcggaggct actgtcgccg aatcggattc gttttaaata gatcttacag tatcactact
     6481 ccatctcagt tcgtgttctt gtcattaatt aacatggatt ataaggatga tgatgataag
     6541 ggagattata aggatgatga tgataaggga gactacaaag acgatgacga caaaatcaca
     6601 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
     6661 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcatat
     6721 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     6781 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
     6841 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     6901 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     6961 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     7021 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
     7081 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     7141 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     7201 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     7261 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     7321 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     7381 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     7441 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat
     7501 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa
     7561 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg
     7621 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat
     7681 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc
     7741 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc
     7801 tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag
     7861 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
     7921 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
     7981 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     8041 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     8101 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag
     8161 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagcat tatgtagtct
     8221 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt
     8281 cagctttctt gtacaaagtg gtgcctaggg cggccgcggt cctgcaactt gaggtagtca
     8341 agatgcataa taaataacgg attgtgtccg taatcacacg tggtgcgtac gataacgcat
     8401 agtgtttttc cctccactta aatcgaaggg ttgtgtcttg gatcgcgcgg gtcaaatgta
     8461 tatggttcat atacatccgc aggcacgtaa taaagcgagg ggttcgaatc cccccgttac
     8521 ccccggtagg ggcccaggta cccggatgtg ttttccgggc tgatgagtcc gtgaggacga
     8581 aacctggctg caggcatgca agcttggcgt aatcatggtc atagcctaga gtccgcaaaa
     8641 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     8701 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     8761 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     8821 ctaaaaccaa aatccagtga cctgcagccc ggccggggga tccactagca gattgtcgtt
     8881 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     8941 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     9001 cgtccatttg tatgtgcatg ccaaccacag gagatctcag taaagcgctg gctgaacccc
     9061 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
     9121 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
     9181 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
     9241 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
     9301 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
     9361 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
     9421 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
     9481 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
     9541 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
     9601 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
     9661 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
     9721 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
     9781 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
     9841 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
     9901 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
     9961 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
    10021 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
    10081 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
    10141 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
    10201 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
    10261 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
    10321 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
    10381 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
    10441 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
    10501 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
    10561 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    10621 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    10681 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    10741 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    10801 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    10861 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    10921 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    10981 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    11041 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    11101 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    11161 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    11221 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    11281 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    11341 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    11401 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    11461 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
    11521 tgatgctgcc aactcgagag cgggccggga gggttcgaga agggggggca ccccccttcg
    11581 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
    11641 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
    11701 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
    11761 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
    11821 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
    11881 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
    11941 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
    12001 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
    12061 gccggcggcc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca
    12121 gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag cgtctagtgg
    12181 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    12241 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
    12301 aacacattgc ggacgttttt aatgtactgg ggtggttttg gtaccgggcc ccccctcgag
    12361 gtcgacggta tcgataagct tgatatcgaa tt
//
