LOCUS       V003164                12338 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003164
VERSION     V003164
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12338)
  AUTHORS   Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
            Rozwadowski K.
  TITLE     TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
            expression vectors for functional analysis of proteins
  JOURNAL   Sci Rep 2, 874 (2012)
   PUBMED   23166857
REFERENCE   2  (bases 1 to 12338)
  AUTHORS   Rozwadowski K, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
            0X2, Canada
REFERENCE   3  (bases 1 to 12338)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12338)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
            874 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..754
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    766..821
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             823..4173
                     /codon_start=1
                     /gene="Rep"
                     /product="replicase"
                     /label="Rep"
                     /protein_id="AFX83491.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     gene            823..4173
                     /gene="Rep"
                     /label="Rep"
     CDS             4249..5667
                     /codon_start=1
                     /gene="RNA pol"
                     /product="RNA polymerase"
                     /label="RNA pol"
                     /protein_id="AFX83492.1"
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
                     IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
                     TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
                     FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
                     KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
                     KVLFRSLFIDGSSC"
     gene            4249..5667
                     /gene="RNA pol"
                     /label="RNA pol"
     CDS             5651..6454
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     CDS             6517..6540
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     protein_bind    6544..6663
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6693..6723
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6777..7433
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7778..8080
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8124..8248)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8489..8533
                     /note="TobRV/G12a_Ribozyme: aligns with Tobacco ringspot
                     virus satellite RNA and Hammerhead ribozyme G12a"
     regulatory      8576..8786
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
     misc_feature    8856..8880
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9035..10180)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10482..11273)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(11485..12120)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    12198..12222
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     12303..12319
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
       61 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      121 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      181 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      241 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      301 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact
      361 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
      421 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
      481 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      541 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
      601 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
      661 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      721 ctctatataa ggaagttcat ttcatttgga gagggtattt ttacaacaat taccaacaac
      781 aacaaacaac agacaacatt acaattacta tttacaatta caatggcata cacacagaca
      841 gctaccacat cagctttgct ggacactgtc cgaggaaaca actccttggt caatgatcta
      901 gcaaagcgtc gtctttacga cacagcggtt gaagagttta acgctcgtga ccgcaggccc
      961 aaggtgaact tttcaaaagt aataagcgag gagcagacgc ttattgctac ccgggcgtat
     1021 ccagaattcc aaattacatt ttataacacg caaaatgccg tgcattcgct tgcaggtgga
     1081 ttgcgatctt tagaactgga atatctgatg atgcaaattc cctacggatc attgacttat
     1141 gacataggcg ggaattttgc atcgcatctg ttcaagggac gagcatatgt acactgctgc
     1201 atgcccaacc tggacgttcg agacatcatg cggcacgaag gccagaaaga cagtattgaa
     1261 ctataccttt ctaggctaga gagagggggg aaaacagtcc ccaacttcca aaaggaagca
     1321 tttgacagat acgcagaaat tcctgaagac gctgtctgtc acaatacttt ccagacatgc
     1381 gaacatcagc cgatgcagca atcaggcaga gtgtatgcca ttgcgctaca cagcatatat
     1441 gacataccag ccgatgagtt cggggcggca ctcttgagga aaaatgtcca tacgtgctat
     1501 gccgctttcc acttctccga gaacctgctt cttgaagatt catgcgtcaa tttggacgaa
     1561 atcaacgcgt gtttttcgcg cgatggagac aagttgacct tttcttttgc atcagagagt
     1621 actcttaatt actgtcatag ttattctaat attcttaagt atgtgtgcaa aacttacttc
     1681 ccggcctcta atagagaggt ttacatgaag gagtttttag tcaccagagt taatacctgg
     1741 ttttgtaagt tttctagaat agatactttt cttttgtaca aaggtgtggc ccataaaagt
     1801 gtagatagtg agcagtttta tactgcaatg gaagacgcat ggcattacaa aaagactctt
     1861 gcaatgtgca acagcgagag aatcctcctt gaggattcat catcagtcaa ttactggttt
     1921 cccaaaatga gggatatggt catcgtacca ttattcgaca tttctttgga gactagtaag
     1981 aggacgcgca aggaagtctt agtgtccaag gatttcgtgt tcacagtgct taaccacatt
     2041 cgaacatacc aggcgaaagc tcttacatac gcaaatgttt tgtccttcgt cgaatcgatt
     2101 cgatcgaggg taatcattaa cggtgtgaca gcgaggtccg aatgggatgt ggacaaatct
     2161 ttgttacaat ccttgtccat gacgttttac ctgcatacta agcttgccgt tctaaaggat
     2221 gacttactga ttagcaagtt tagtctcggt tcgaaaacgg tgtgccagca tgtgtgggat
     2281 gagatttcgc tggcgtttgg gaacgcattt ccctccgtga aagagaggct cttgaacagg
     2341 aaacttatca gagtggcagg cgacgcatta gagatcaggg tgcctgatct atatgtgacc
     2401 ttccacgaca gattagtgac tgagtacaag gcctctgtgg acatgcctgc gcttgacatt
     2461 aggaagaaga tggaagaaac ggaagtgatg tacaatgcac tttcagaatt atcggtgtta
     2521 agggagtctg acaaattcga tgttgatgtt ttttcccaga tgtgccaatc tttggaagtt
     2581 gacccaatga cggcagcgaa ggttatagtc gcggtcatga gcaatgagag cggtctgact
     2641 ctcacatttg aacgacctac tgaggcgaat gttgcgctag ctttacagga tcaagagaag
     2701 gcttcagaag gtgcattggt agttacctca agagaagttg aagaaccgtc catgaagggt
     2761 tcgatggcca gaggagagtt acaattagct ggtcttgctg gagatcatcc ggaatcgtcc
     2821 tattctaaga acgaggagat agagtcttta gagcagtttc atatggcgac ggcagattcg
     2881 ttaattcgta agcagatgag ctcgattgtg tacacgggtc cgattaaagt tcagcaaatg
     2941 aaaaacttta tcgatagcct ggtagcatca ctatctgctg cggtgtcgaa tctcgtcaag
     3001 atcctcaaag atacagctgc tattgacctt gaaacccgtc aaaagtttgg agtcttggat
     3061 gttgcatcta ggaagtggtt aatcaaacca acggccaaga gtcatgcatg gggtgttgtt
     3121 gaaacccacg cgaggaagta tcatgtggcg cttttggaat atgatgagca gggtgtggtg
     3181 acatgcgatg attggagaag agtagctgtt agctctgagt ctgttgttta ttccgacatg
     3241 gcgaaactca gaactctgcg cagactgctt cgaaacggag aaccgcatgt cagtagcgca
     3301 aaggttgttc ttgtggacgg agttccgggc tgtggaaaaa ccaaagaaat tctttccagg
     3361 gttaattttg atgaagatct aattttagta cctgggaagc aagccgcgga aatgatcaga
     3421 agacgtgcga attcctcagg gattattgtg gccacgaagg acaacgttaa aaccgttgat
     3481 tctttcatga tgaattttgg gaaaagcaca cgctgtcagt tcaagaggtt attcattgat
     3541 gaagggttga tgttgcatac tggttgtgtt aattttcttg tggcgatgtc attgtgcgaa
     3601 attgcatatg tttacggaga cacacagcag attccataca tcaatagagt ttcaggattc
     3661 ccgtaccccg cccattttgc caaattggaa gttgacgagg tggagacacg cagaactact
     3721 ctccgttgtc cagccgatgt cacacattat ctgaacagga gatatgaggg ctttgtcatg
     3781 agcacttctt cggttaaaaa gtctgtttcg caggagatgg tcggcggagc cgccgtgatc
     3841 aatccgatct caaaaccctt gcatggcaag atcctgactt ttacccaatc ggataaagaa
     3901 gctctgcttt caagagggta ttcagatgtt cacactgtgc atgaagtgca aggcgagaca
     3961 tactctgatg tttcactagt taggttaacc cctacaccgg tctccatcat tgcaggagac
     4021 agcccacatg ttttggtcgc attgtcaagg cacacctgtt cgctcaagta ctacactgtt
     4081 gttatggatc ctttagttag tatcattaga gatctagaga aacttagctc gtacttgtta
     4141 gatatgtata aggtcgatgc aggaacacaa tagcaattac agattgactc ggtgttcaaa
     4201 ggttccaatc tttttgttgc agcgccaaag actggtgata tttctgatat gcagttttac
     4261 tatgataagt gtctcccagg caacagcacc atgatgaata attttgatgc tgttaccatg
     4321 aggttgactg acatttcatt gaatgtcaaa gattgcatat tggatatgtc taagtctgtt
     4381 gctgcgccta aggatcaaat caaaccacta atacctatgg tacgaacggc ggcagaaatg
     4441 ccacgccaga ctggactatt ggaaaattta gtggcgatga ttaaaagaaa ctttaacgca
     4501 cccgagttgt ctggcatcat tgatattgaa aatactgcat ctttggttgt agataagttt
     4561 tttgatagtt atttgcttaa agaaaaaaga aaaccaaata aaaatgtttc tttgttcagt
     4621 agagagtctc tcaatagatg gttagaaaag caggaacagg taacaatagg ccagctcgca
     4681 gattttgatt ttgtggattt gccagcagtt gatcagtaca gacacatgat taaagcacaa
     4741 cccaaacaaa agttggacac ttcaatccaa acggagtacc cggctttgca gacgattgtg
     4801 taccatcaaa aaagatcaat gcaatatcgc ccgttgttag tgagcttacc aggcaattac
     4861 tggacagtgt tgattcgagc agattttgtt ttcacaagaa agacaccagc gcagattgag
     4921 gatttcttcg gagatctcga cagtcatgtg ccgatggatg tcttggagct ggatatatca
     4981 aaatacgaca aatctcagaa tgaattccac tgtgcagtag aatacgagat ctggcgaaga
     5041 ttgggtttcg aagacttctt gggagaagtt tggaaacaag ggcatagaaa gaccaccctc
     5101 aaggattata ccgcaggtat aaaaacttgc atctggtatc aaagaaagag cggggacgtc
     5161 acgacgttca ttggaaacac tgtgatcatt gctgcatgtt tggcctcgat gcttccgatg
     5221 gagaaaataa tcaaaggagc cttttgcggt gacgatagtc tgctgtactt tccaaagggt
     5281 tgtgagtttc cggatgtgca acactccgcg aatcttatgt ggaattttga agcaaaactg
     5341 tttaaaaaac agtatggata cttttgcgga agatatgtaa tacatcacga cagaggatgc
     5401 attgtgtatt acgatcccct aaagttgatc tcgaaacttg gtgctaaaca catcaaggat
     5461 tgggaacact tggaggagtt cagaaggtct ctttgtgatg ttgctgtttc gttgaacaat
     5521 tgtgcgtatt acacacagtt ggacgacgct gtatgggagg ttcataagac cgcccctcca
     5581 ggttcgtttg tttataaaag tctggtgaag tatttgtctg ataaagttct ttttagaagt
     5641 ttgtttatag atggctctag ttgttaaagg aaaagtgaat atcaatgagt ttatcgacct
     5701 gacaaaaatg gagaagatct taccgtcgat gtttacccct gtaaagagtg ttatgtgttc
     5761 caaagttgat aaaataatgg ttcatgagaa tgagtcattg tcaggggtga accttcttaa
     5821 aggagttaag cttattgata gtggatacgt ctgtttagcc ggtttggtcg tcacgggcga
     5881 gtggaacttg cctgacaatt gcagaggagg tgtgagcgtg tgtctggtgg acaaaaggat
     5941 ggaaagagcc gacgaggcca ttctcggatc ttactacaca gcagctgcaa agaaaagatt
     6001 tcagttcaag gtcgttccca attatgctat aaccacccag gacgcgatga aaaacgtctg
     6061 gcaagtttta gttaatatta gaaatgtgaa gatgtcagcg ggtttctgtc cgctttctct
     6121 ggagtttgtg tcggtgtgta ttgtttatag aaataatata aaattaggtt tgagagagaa
     6181 gattacaaac gtgagagacg gagggcccat ggaacttaca gaagaagtcg ttgatgagtt
     6241 catggaagat gtccctatgt cgatcaggct tgcaaagttt cgatctcgaa ccggaaaaaa
     6301 gagtgatgtc cgcaaaggga aaaatagtag tagtgatcgg tcagtgccga acaagaacta
     6361 tagaaatgtt aaggattttg ggggaatgag ttttaaaaag aataatttaa tcgatgatga
     6421 ttcggaggct actgtcgccg aatcggattc gttttaaata gatcttacag tatcactact
     6481 ccatctcagt tcgtgttctt gtcattaatt aacatggact acaaagacga tgacgacaaa
     6541 atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     6601 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     6661 tcatattggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     6721 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     6781 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     6841 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     6901 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     6961 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     7021 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     7081 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     7141 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     7201 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     7261 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     7321 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     7381 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc
     7441 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     7501 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
     7561 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     7621 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     7681 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     7741 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
     7801 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     7861 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     7921 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     7981 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     8041 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     8101 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagcattatg
     8161 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     8221 ctcgttcagc tttcttgtac aaagtggtgc ctagggcggc cgcggtcctg caacttgagg
     8281 tagtcaagat gcataataaa taacggattg tgtccgtaat cacacgtggt gcgtacgata
     8341 acgcatagtg tttttccctc cacttaaatc gaagggttgt gtcttggatc gcgcgggtca
     8401 aatgtatatg gttcatatac atccgcaggc acgtaataaa gcgaggggtt cgaatccccc
     8461 cgttaccccc ggtaggggcc caggtacccg gatgtgtttt ccgggctgat gagtccgtga
     8521 ggacgaaacc tggctgcagg catgcaagct tggcgtaatc atggtcatag cctagagtcc
     8581 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     8641 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     8701 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     8761 taattcctaa aaccaaaatc cagtgacctg cagcccggcc gggggatcca ctagcagatt
     8821 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     8881 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     8941 tccgttcgtc catttgtatg tgcatgccaa ccacaggaga tctcagtaaa gcgctggctg
     9001 aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat
     9061 tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct
     9121 gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca
     9181 gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg
     9241 gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca
     9301 cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga
     9361 cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc
     9421 ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat
     9481 tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga
     9541 taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc
     9601 gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt
     9661 tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg
     9721 tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct
     9781 gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga
     9841 cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg
     9901 tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct
     9961 ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga
    10021 tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac
    10081 gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca
    10141 gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg
    10201 ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg
    10261 tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct
    10321 tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat
    10381 acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct
    10441 cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc atccagtaaa
    10501 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
    10561 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
    10621 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
    10681 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
    10741 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    10801 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    10861 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    10921 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    10981 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    11041 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    11101 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    11161 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    11221 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    11281 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    11341 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    11401 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat
    11461 tatgggtgat gctgccaact cgagagcggg ccgggagggt tcgagaaggg ggggcacccc
    11521 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
    11581 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
    11641 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
    11701 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
    11761 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
    11821 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
    11881 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
    11941 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
    12001 cacaacgccg gcggccggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg
    12061 tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc
    12121 tagtggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    12181 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    12241 cttaataaca cattgcggac gtttttaatg tactggggtg gttttggtac cgggcccccc
    12301 ctcgaggtcg acggtatcga taagcttgat atcgaatt
//
