LOCUS       V003165                12404 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003165
VERSION     V003165
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
            Rozwadowski K.
  TITLE     TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
            expression vectors for functional analysis of proteins
  JOURNAL   Sci Rep 2, 874 (2012)
   PUBMED   23166857
REFERENCE   2  (bases 1 to 12404)
  AUTHORS   Rozwadowski K, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
            0X2, Canada
REFERENCE   3  (bases 1 to 12404)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
            874 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..754
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    766..821
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             823..4173
                     /codon_start=1
                     /gene="Rep"
                     /product="replicase"
                     /label="Rep"
                     /protein_id="AFX83484.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     gene            823..4173
                     /gene="Rep"
                     /label="Rep"
     CDS             4249..5667
                     /codon_start=1
                     /gene="RNA pol"
                     /product="RNA polymerase"
                     /label="RNA pol"
                     /protein_id="AFX83485.1"
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
                     IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
                     TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
                     FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
                     KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
                     KVLFRSLFIDGSSC"
     gene            4249..5667
                     /gene="RNA pol"
                     /label="RNA pol"
     CDS             5651..6454
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     CDS             6517..6606
                     /label="3xHA"
                     /note="three tandem HA epitope tags"
     protein_bind    6610..6729
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6759..6789
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6843..7499
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7844..8146
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8190..8314)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8555..8599
                     /note="TobRV/G12a_Ribozyme: aligns with Tobacco ringspot
                     virus satellite RNA and Hammerhead ribozyme G12a"
     regulatory      8642..8852
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
     misc_feature    8922..8946
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9101..10246)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10548..11339)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(11551..12186)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    12264..12288
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     12369..12385
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
       61 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      121 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      181 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      241 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      301 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact
      361 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
      421 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
      481 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      541 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
      601 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
      661 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      721 ctctatataa ggaagttcat ttcatttgga gagggtattt ttacaacaat taccaacaac
      781 aacaaacaac agacaacatt acaattacta tttacaatta caatggcata cacacagaca
      841 gctaccacat cagctttgct ggacactgtc cgaggaaaca actccttggt caatgatcta
      901 gcaaagcgtc gtctttacga cacagcggtt gaagagttta acgctcgtga ccgcaggccc
      961 aaggtgaact tttcaaaagt aataagcgag gagcagacgc ttattgctac ccgggcgtat
     1021 ccagaattcc aaattacatt ttataacacg caaaatgccg tgcattcgct tgcaggtgga
     1081 ttgcgatctt tagaactgga atatctgatg atgcaaattc cctacggatc attgacttat
     1141 gacataggcg ggaattttgc atcgcatctg ttcaagggac gagcatatgt acactgctgc
     1201 atgcccaacc tggacgttcg agacatcatg cggcacgaag gccagaaaga cagtattgaa
     1261 ctataccttt ctaggctaga gagagggggg aaaacagtcc ccaacttcca aaaggaagca
     1321 tttgacagat acgcagaaat tcctgaagac gctgtctgtc acaatacttt ccagacatgc
     1381 gaacatcagc cgatgcagca atcaggcaga gtgtatgcca ttgcgctaca cagcatatat
     1441 gacataccag ccgatgagtt cggggcggca ctcttgagga aaaatgtcca tacgtgctat
     1501 gccgctttcc acttctccga gaacctgctt cttgaagatt catgcgtcaa tttggacgaa
     1561 atcaacgcgt gtttttcgcg cgatggagac aagttgacct tttcttttgc atcagagagt
     1621 actcttaatt actgtcatag ttattctaat attcttaagt atgtgtgcaa aacttacttc
     1681 ccggcctcta atagagaggt ttacatgaag gagtttttag tcaccagagt taatacctgg
     1741 ttttgtaagt tttctagaat agatactttt cttttgtaca aaggtgtggc ccataaaagt
     1801 gtagatagtg agcagtttta tactgcaatg gaagacgcat ggcattacaa aaagactctt
     1861 gcaatgtgca acagcgagag aatcctcctt gaggattcat catcagtcaa ttactggttt
     1921 cccaaaatga gggatatggt catcgtacca ttattcgaca tttctttgga gactagtaag
     1981 aggacgcgca aggaagtctt agtgtccaag gatttcgtgt tcacagtgct taaccacatt
     2041 cgaacatacc aggcgaaagc tcttacatac gcaaatgttt tgtccttcgt cgaatcgatt
     2101 cgatcgaggg taatcattaa cggtgtgaca gcgaggtccg aatgggatgt ggacaaatct
     2161 ttgttacaat ccttgtccat gacgttttac ctgcatacta agcttgccgt tctaaaggat
     2221 gacttactga ttagcaagtt tagtctcggt tcgaaaacgg tgtgccagca tgtgtgggat
     2281 gagatttcgc tggcgtttgg gaacgcattt ccctccgtga aagagaggct cttgaacagg
     2341 aaacttatca gagtggcagg cgacgcatta gagatcaggg tgcctgatct atatgtgacc
     2401 ttccacgaca gattagtgac tgagtacaag gcctctgtgg acatgcctgc gcttgacatt
     2461 aggaagaaga tggaagaaac ggaagtgatg tacaatgcac tttcagaatt atcggtgtta
     2521 agggagtctg acaaattcga tgttgatgtt ttttcccaga tgtgccaatc tttggaagtt
     2581 gacccaatga cggcagcgaa ggttatagtc gcggtcatga gcaatgagag cggtctgact
     2641 ctcacatttg aacgacctac tgaggcgaat gttgcgctag ctttacagga tcaagagaag
     2701 gcttcagaag gtgcattggt agttacctca agagaagttg aagaaccgtc catgaagggt
     2761 tcgatggcca gaggagagtt acaattagct ggtcttgctg gagatcatcc ggaatcgtcc
     2821 tattctaaga acgaggagat agagtcttta gagcagtttc atatggcgac ggcagattcg
     2881 ttaattcgta agcagatgag ctcgattgtg tacacgggtc cgattaaagt tcagcaaatg
     2941 aaaaacttta tcgatagcct ggtagcatca ctatctgctg cggtgtcgaa tctcgtcaag
     3001 atcctcaaag atacagctgc tattgacctt gaaacccgtc aaaagtttgg agtcttggat
     3061 gttgcatcta ggaagtggtt aatcaaacca acggccaaga gtcatgcatg gggtgttgtt
     3121 gaaacccacg cgaggaagta tcatgtggcg cttttggaat atgatgagca gggtgtggtg
     3181 acatgcgatg attggagaag agtagctgtt agctctgagt ctgttgttta ttccgacatg
     3241 gcgaaactca gaactctgcg cagactgctt cgaaacggag aaccgcatgt cagtagcgca
     3301 aaggttgttc ttgtggacgg agttccgggc tgtggaaaaa ccaaagaaat tctttccagg
     3361 gttaattttg atgaagatct aattttagta cctgggaagc aagccgcgga aatgatcaga
     3421 agacgtgcga attcctcagg gattattgtg gccacgaagg acaacgttaa aaccgttgat
     3481 tctttcatga tgaattttgg gaaaagcaca cgctgtcagt tcaagaggtt attcattgat
     3541 gaagggttga tgttgcatac tggttgtgtt aattttcttg tggcgatgtc attgtgcgaa
     3601 attgcatatg tttacggaga cacacagcag attccataca tcaatagagt ttcaggattc
     3661 ccgtaccccg cccattttgc caaattggaa gttgacgagg tggagacacg cagaactact
     3721 ctccgttgtc cagccgatgt cacacattat ctgaacagga gatatgaggg ctttgtcatg
     3781 agcacttctt cggttaaaaa gtctgtttcg caggagatgg tcggcggagc cgccgtgatc
     3841 aatccgatct caaaaccctt gcatggcaag atcctgactt ttacccaatc ggataaagaa
     3901 gctctgcttt caagagggta ttcagatgtt cacactgtgc atgaagtgca aggcgagaca
     3961 tactctgatg tttcactagt taggttaacc cctacaccgg tctccatcat tgcaggagac
     4021 agcccacatg ttttggtcgc attgtcaagg cacacctgtt cgctcaagta ctacactgtt
     4081 gttatggatc ctttagttag tatcattaga gatctagaga aacttagctc gtacttgtta
     4141 gatatgtata aggtcgatgc aggaacacaa tagcaattac agattgactc ggtgttcaaa
     4201 ggttccaatc tttttgttgc agcgccaaag actggtgata tttctgatat gcagttttac
     4261 tatgataagt gtctcccagg caacagcacc atgatgaata attttgatgc tgttaccatg
     4321 aggttgactg acatttcatt gaatgtcaaa gattgcatat tggatatgtc taagtctgtt
     4381 gctgcgccta aggatcaaat caaaccacta atacctatgg tacgaacggc ggcagaaatg
     4441 ccacgccaga ctggactatt ggaaaattta gtggcgatga ttaaaagaaa ctttaacgca
     4501 cccgagttgt ctggcatcat tgatattgaa aatactgcat ctttggttgt agataagttt
     4561 tttgatagtt atttgcttaa agaaaaaaga aaaccaaata aaaatgtttc tttgttcagt
     4621 agagagtctc tcaatagatg gttagaaaag caggaacagg taacaatagg ccagctcgca
     4681 gattttgatt ttgtggattt gccagcagtt gatcagtaca gacacatgat taaagcacaa
     4741 cccaaacaaa agttggacac ttcaatccaa acggagtacc cggctttgca gacgattgtg
     4801 taccatcaaa aaagatcaat gcaatatcgc ccgttgttag tgagcttacc aggcaattac
     4861 tggacagtgt tgattcgagc agattttgtt ttcacaagaa agacaccagc gcagattgag
     4921 gatttcttcg gagatctcga cagtcatgtg ccgatggatg tcttggagct ggatatatca
     4981 aaatacgaca aatctcagaa tgaattccac tgtgcagtag aatacgagat ctggcgaaga
     5041 ttgggtttcg aagacttctt gggagaagtt tggaaacaag ggcatagaaa gaccaccctc
     5101 aaggattata ccgcaggtat aaaaacttgc atctggtatc aaagaaagag cggggacgtc
     5161 acgacgttca ttggaaacac tgtgatcatt gctgcatgtt tggcctcgat gcttccgatg
     5221 gagaaaataa tcaaaggagc cttttgcggt gacgatagtc tgctgtactt tccaaagggt
     5281 tgtgagtttc cggatgtgca acactccgcg aatcttatgt ggaattttga agcaaaactg
     5341 tttaaaaaac agtatggata cttttgcgga agatatgtaa tacatcacga cagaggatgc
     5401 attgtgtatt acgatcccct aaagttgatc tcgaaacttg gtgctaaaca catcaaggat
     5461 tgggaacact tggaggagtt cagaaggtct ctttgtgatg ttgctgtttc gttgaacaat
     5521 tgtgcgtatt acacacagtt ggacgacgct gtatgggagg ttcataagac cgcccctcca
     5581 ggttcgtttg tttataaaag tctggtgaag tatttgtctg ataaagttct ttttagaagt
     5641 ttgtttatag atggctctag ttgttaaagg aaaagtgaat atcaatgagt ttatcgacct
     5701 gacaaaaatg gagaagatct taccgtcgat gtttacccct gtaaagagtg ttatgtgttc
     5761 caaagttgat aaaataatgg ttcatgagaa tgagtcattg tcaggggtga accttcttaa
     5821 aggagttaag cttattgata gtggatacgt ctgtttagcc ggtttggtcg tcacgggcga
     5881 gtggaacttg cctgacaatt gcagaggagg tgtgagcgtg tgtctggtgg acaaaaggat
     5941 ggaaagagcc gacgaggcca ttctcggatc ttactacaca gcagctgcaa agaaaagatt
     6001 tcagttcaag gtcgttccca attatgctat aaccacccag gacgcgatga aaaacgtctg
     6061 gcaagtttta gttaatatta gaaatgtgaa gatgtcagcg ggtttctgtc cgctttctct
     6121 ggagtttgtg tcggtgtgta ttgtttatag aaataatata aaattaggtt tgagagagaa
     6181 gattacaaac gtgagagacg gagggcccat ggaacttaca gaagaagtcg ttgatgagtt
     6241 catggaagat gtccctatgt cgatcaggct tgcaaagttt cgatctcgaa ccggaaaaaa
     6301 gagtgatgtc cgcaaaggga aaaatagtag tagtgatcgg tcagtgccga acaagaacta
     6361 tagaaatgtt aaggattttg ggggaatgag ttttaaaaag aataatttaa tcgatgatga
     6421 ttcggaggct actgtcgccg aatcggattc gttttaaata gatcttacag tatcactact
     6481 ccatctcagt tcgtgttctt gtcattaatt aacatgtatc cttatgatgt tcctgattat
     6541 gctggttatc cttatgatgt tcctgattat gctggatctt acccatacga tgttccagat
     6601 tacgctatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     6661 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     6721 atccagtcat attggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     6781 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     6841 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     6901 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     6961 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     7021 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     7081 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     7141 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     7201 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     7261 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     7321 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     7381 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     7441 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     7501 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     7561 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     7621 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     7681 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     7741 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     7801 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     7861 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     7921 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     7981 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     8041 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     8101 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     8161 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagc
     8221 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     8281 acgtttctcg ttcagctttc ttgtacaaag tggtgcctag ggcggccgcg gtcctgcaac
     8341 ttgaggtagt caagatgcat aataaataac ggattgtgtc cgtaatcaca cgtggtgcgt
     8401 acgataacgc atagtgtttt tccctccact taaatcgaag ggttgtgtct tggatcgcgc
     8461 gggtcaaatg tatatggttc atatacatcc gcaggcacgt aataaagcga ggggttcgaa
     8521 tccccccgtt acccccggta ggggcccagg tacccggatg tgttttccgg gctgatgagt
     8581 ccgtgaggac gaaacctggc tgcaggcatg caagcttggc gtaatcatgg tcatagccta
     8641 gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga
     8701 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt
     8761 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     8821 aatttctaat tcctaaaacc aaaatccagt gacctgcagc ccggccgggg gatccactag
     8881 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
     8941 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     9001 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac aggagatctc agtaaagcgc
     9061 tggctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt
     9121 catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc
     9181 cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg
     9241 tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg
     9301 ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa
     9361 acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt
     9421 ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg
     9481 tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc
     9541 ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct
     9601 cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt
     9661 cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga
     9721 ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc
     9781 gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg
     9841 aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct
     9901 cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc
     9961 ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg
    10021 aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc
    10081 gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg
    10141 gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt
    10201 cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg
    10261 ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg
    10321 ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc
    10381 cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc gaccccttgc
    10441 ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg
    10501 tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa caattcatcc
    10561 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
    10621 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg
    10681 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca
    10741 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg
    10801 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct
    10861 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct
    10921 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc
    10981 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct
    11041 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt
    11101 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt
    11161 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg
    11221 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt
    11281 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccatt
    11341 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
    11401 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
    11461 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
    11521 cacaattatg ggtgatgctg ccaactcgag agcgggccgg gagggttcga gaaggggggg
    11581 cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt
    11641 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc
    11701 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt
    11761 gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt
    11821 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca
    11881 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc
    11941 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt
    12001 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga
    12061 ggtatccaca acgccggcgg ccggccgcgg tgtctcgcac acggcttcga cggcgtttct
    12121 ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg
    12181 agcgtctagt ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    12241 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
    12301 agacaactta ataacacatt gcggacgttt ttaatgtact ggggtggttt tggtaccggg
    12361 ccccccctcg aggtcgacgg tatcgataag cttgatatcg aatt
//
