LOCUS       V003166                12341 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003166
VERSION     V003166
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
            Rozwadowski K.
  TITLE     TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
            expression vectors for functional analysis of proteins
  JOURNAL   Sci Rep 2, 874 (2012)
   PUBMED   23166857
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   Rozwadowski K, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
            0X2, Canada
REFERENCE   3  (bases 1 to 12341)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12341)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
            874 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..754
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    766..821
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             823..4173
                     /codon_start=1
                     /gene="Rep"
                     /product="replicase"
                     /label="Rep"
                     /protein_id="AFX83478.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     gene            823..4173
                     /gene="Rep"
                     /label="Rep"
     CDS             4249..5667
                     /codon_start=1
                     /gene="RNA pol"
                     /product="RNA polymerase"
                     /label="RNA pol"
                     /protein_id="AFX83477.1"
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
                     IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
                     TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
                     FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
                     KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
                     KVLFRSLFIDGSSC"
     gene            4249..5667
                     /gene="RNA pol"
                     /label="RNA pol"
     CDS             5651..6454
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     CDS             6517..6543
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    6547..6666
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6696..6726
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6780..7436
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7781..8083
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8127..8251)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8492..8536
                     /note="TobRV/G12a_Ribozyme: aligns with Tobacco ringspot
                     virus satellite RNA and Hammerhead ribozyme G12a"
     regulatory      8579..8789
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
     misc_feature    8859..8883
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9038..10183)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10485..11276)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(11488..12123)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    12201..12225
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     12306..12322
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cctgcaggtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
       61 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      121 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      181 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      241 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      301 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact
      361 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
      421 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
      481 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      541 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
      601 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
      661 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      721 ctctatataa ggaagttcat ttcatttgga gagggtattt ttacaacaat taccaacaac
      781 aacaaacaac agacaacatt acaattacta tttacaatta caatggcata cacacagaca
      841 gctaccacat cagctttgct ggacactgtc cgaggaaaca actccttggt caatgatcta
      901 gcaaagcgtc gtctttacga cacagcggtt gaagagttta acgctcgtga ccgcaggccc
      961 aaggtgaact tttcaaaagt aataagcgag gagcagacgc ttattgctac ccgggcgtat
     1021 ccagaattcc aaattacatt ttataacacg caaaatgccg tgcattcgct tgcaggtgga
     1081 ttgcgatctt tagaactgga atatctgatg atgcaaattc cctacggatc attgacttat
     1141 gacataggcg ggaattttgc atcgcatctg ttcaagggac gagcatatgt acactgctgc
     1201 atgcccaacc tggacgttcg agacatcatg cggcacgaag gccagaaaga cagtattgaa
     1261 ctataccttt ctaggctaga gagagggggg aaaacagtcc ccaacttcca aaaggaagca
     1321 tttgacagat acgcagaaat tcctgaagac gctgtctgtc acaatacttt ccagacatgc
     1381 gaacatcagc cgatgcagca atcaggcaga gtgtatgcca ttgcgctaca cagcatatat
     1441 gacataccag ccgatgagtt cggggcggca ctcttgagga aaaatgtcca tacgtgctat
     1501 gccgctttcc acttctccga gaacctgctt cttgaagatt catgcgtcaa tttggacgaa
     1561 atcaacgcgt gtttttcgcg cgatggagac aagttgacct tttcttttgc atcagagagt
     1621 actcttaatt actgtcatag ttattctaat attcttaagt atgtgtgcaa aacttacttc
     1681 ccggcctcta atagagaggt ttacatgaag gagtttttag tcaccagagt taatacctgg
     1741 ttttgtaagt tttctagaat agatactttt cttttgtaca aaggtgtggc ccataaaagt
     1801 gtagatagtg agcagtttta tactgcaatg gaagacgcat ggcattacaa aaagactctt
     1861 gcaatgtgca acagcgagag aatcctcctt gaggattcat catcagtcaa ttactggttt
     1921 cccaaaatga gggatatggt catcgtacca ttattcgaca tttctttgga gactagtaag
     1981 aggacgcgca aggaagtctt agtgtccaag gatttcgtgt tcacagtgct taaccacatt
     2041 cgaacatacc aggcgaaagc tcttacatac gcaaatgttt tgtccttcgt cgaatcgatt
     2101 cgatcgaggg taatcattaa cggtgtgaca gcgaggtccg aatgggatgt ggacaaatct
     2161 ttgttacaat ccttgtccat gacgttttac ctgcatacta agcttgccgt tctaaaggat
     2221 gacttactga ttagcaagtt tagtctcggt tcgaaaacgg tgtgccagca tgtgtgggat
     2281 gagatttcgc tggcgtttgg gaacgcattt ccctccgtga aagagaggct cttgaacagg
     2341 aaacttatca gagtggcagg cgacgcatta gagatcaggg tgcctgatct atatgtgacc
     2401 ttccacgaca gattagtgac tgagtacaag gcctctgtgg acatgcctgc gcttgacatt
     2461 aggaagaaga tggaagaaac ggaagtgatg tacaatgcac tttcagaatt atcggtgtta
     2521 agggagtctg acaaattcga tgttgatgtt ttttcccaga tgtgccaatc tttggaagtt
     2581 gacccaatga cggcagcgaa ggttatagtc gcggtcatga gcaatgagag cggtctgact
     2641 ctcacatttg aacgacctac tgaggcgaat gttgcgctag ctttacagga tcaagagaag
     2701 gcttcagaag gtgcattggt agttacctca agagaagttg aagaaccgtc catgaagggt
     2761 tcgatggcca gaggagagtt acaattagct ggtcttgctg gagatcatcc ggaatcgtcc
     2821 tattctaaga acgaggagat agagtcttta gagcagtttc atatggcgac ggcagattcg
     2881 ttaattcgta agcagatgag ctcgattgtg tacacgggtc cgattaaagt tcagcaaatg
     2941 aaaaacttta tcgatagcct ggtagcatca ctatctgctg cggtgtcgaa tctcgtcaag
     3001 atcctcaaag atacagctgc tattgacctt gaaacccgtc aaaagtttgg agtcttggat
     3061 gttgcatcta ggaagtggtt aatcaaacca acggccaaga gtcatgcatg gggtgttgtt
     3121 gaaacccacg cgaggaagta tcatgtggcg cttttggaat atgatgagca gggtgtggtg
     3181 acatgcgatg attggagaag agtagctgtt agctctgagt ctgttgttta ttccgacatg
     3241 gcgaaactca gaactctgcg cagactgctt cgaaacggag aaccgcatgt cagtagcgca
     3301 aaggttgttc ttgtggacgg agttccgggc tgtggaaaaa ccaaagaaat tctttccagg
     3361 gttaattttg atgaagatct aattttagta cctgggaagc aagccgcgga aatgatcaga
     3421 agacgtgcga attcctcagg gattattgtg gccacgaagg acaacgttaa aaccgttgat
     3481 tctttcatga tgaattttgg gaaaagcaca cgctgtcagt tcaagaggtt attcattgat
     3541 gaagggttga tgttgcatac tggttgtgtt aattttcttg tggcgatgtc attgtgcgaa
     3601 attgcatatg tttacggaga cacacagcag attccataca tcaatagagt ttcaggattc
     3661 ccgtaccccg cccattttgc caaattggaa gttgacgagg tggagacacg cagaactact
     3721 ctccgttgtc cagccgatgt cacacattat ctgaacagga gatatgaggg ctttgtcatg
     3781 agcacttctt cggttaaaaa gtctgtttcg caggagatgg tcggcggagc cgccgtgatc
     3841 aatccgatct caaaaccctt gcatggcaag atcctgactt ttacccaatc ggataaagaa
     3901 gctctgcttt caagagggta ttcagatgtt cacactgtgc atgaagtgca aggcgagaca
     3961 tactctgatg tttcactagt taggttaacc cctacaccgg tctccatcat tgcaggagac
     4021 agcccacatg ttttggtcgc attgtcaagg cacacctgtt cgctcaagta ctacactgtt
     4081 gttatggatc ctttagttag tatcattaga gatctagaga aacttagctc gtacttgtta
     4141 gatatgtata aggtcgatgc aggaacacaa tagcaattac agattgactc ggtgttcaaa
     4201 ggttccaatc tttttgttgc agcgccaaag actggtgata tttctgatat gcagttttac
     4261 tatgataagt gtctcccagg caacagcacc atgatgaata attttgatgc tgttaccatg
     4321 aggttgactg acatttcatt gaatgtcaaa gattgcatat tggatatgtc taagtctgtt
     4381 gctgcgccta aggatcaaat caaaccacta atacctatgg tacgaacggc ggcagaaatg
     4441 ccacgccaga ctggactatt ggaaaattta gtggcgatga ttaaaagaaa ctttaacgca
     4501 cccgagttgt ctggcatcat tgatattgaa aatactgcat ctttggttgt agataagttt
     4561 tttgatagtt atttgcttaa agaaaaaaga aaaccaaata aaaatgtttc tttgttcagt
     4621 agagagtctc tcaatagatg gttagaaaag caggaacagg taacaatagg ccagctcgca
     4681 gattttgatt ttgtggattt gccagcagtt gatcagtaca gacacatgat taaagcacaa
     4741 cccaaacaaa agttggacac ttcaatccaa acggagtacc cggctttgca gacgattgtg
     4801 taccatcaaa aaagatcaat gcaatatcgc ccgttgttag tgagcttacc aggcaattac
     4861 tggacagtgt tgattcgagc agattttgtt ttcacaagaa agacaccagc gcagattgag
     4921 gatttcttcg gagatctcga cagtcatgtg ccgatggatg tcttggagct ggatatatca
     4981 aaatacgaca aatctcagaa tgaattccac tgtgcagtag aatacgagat ctggcgaaga
     5041 ttgggtttcg aagacttctt gggagaagtt tggaaacaag ggcatagaaa gaccaccctc
     5101 aaggattata ccgcaggtat aaaaacttgc atctggtatc aaagaaagag cggggacgtc
     5161 acgacgttca ttggaaacac tgtgatcatt gctgcatgtt tggcctcgat gcttccgatg
     5221 gagaaaataa tcaaaggagc cttttgcggt gacgatagtc tgctgtactt tccaaagggt
     5281 tgtgagtttc cggatgtgca acactccgcg aatcttatgt ggaattttga agcaaaactg
     5341 tttaaaaaac agtatggata cttttgcgga agatatgtaa tacatcacga cagaggatgc
     5401 attgtgtatt acgatcccct aaagttgatc tcgaaacttg gtgctaaaca catcaaggat
     5461 tgggaacact tggaggagtt cagaaggtct ctttgtgatg ttgctgtttc gttgaacaat
     5521 tgtgcgtatt acacacagtt ggacgacgct gtatgggagg ttcataagac cgcccctcca
     5581 ggttcgtttg tttataaaag tctggtgaag tatttgtctg ataaagttct ttttagaagt
     5641 ttgtttatag atggctctag ttgttaaagg aaaagtgaat atcaatgagt ttatcgacct
     5701 gacaaaaatg gagaagatct taccgtcgat gtttacccct gtaaagagtg ttatgtgttc
     5761 caaagttgat aaaataatgg ttcatgagaa tgagtcattg tcaggggtga accttcttaa
     5821 aggagttaag cttattgata gtggatacgt ctgtttagcc ggtttggtcg tcacgggcga
     5881 gtggaacttg cctgacaatt gcagaggagg tgtgagcgtg tgtctggtgg acaaaaggat
     5941 ggaaagagcc gacgaggcca ttctcggatc ttactacaca gcagctgcaa agaaaagatt
     6001 tcagttcaag gtcgttccca attatgctat aaccacccag gacgcgatga aaaacgtctg
     6061 gcaagtttta gttaatatta gaaatgtgaa gatgtcagcg ggtttctgtc cgctttctct
     6121 ggagtttgtg tcggtgtgta ttgtttatag aaataatata aaattaggtt tgagagagaa
     6181 gattacaaac gtgagagacg gagggcccat ggaacttaca gaagaagtcg ttgatgagtt
     6241 catggaagat gtccctatgt cgatcaggct tgcaaagttt cgatctcgaa ccggaaaaaa
     6301 gagtgatgtc cgcaaaggga aaaatagtag tagtgatcgg tcagtgccga acaagaacta
     6361 tagaaatgtt aaggattttg ggggaatgag ttttaaaaag aataatttaa tcgatgatga
     6421 ttcggaggct actgtcgccg aatcggattc gttttaaata gatcttacag tatcactact
     6481 ccatctcagt tcgtgttctt gtcattaatt aacatgtacc catacgatgt tccagattac
     6541 gctatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     6601 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     6661 cagtcatatt ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     6721 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     6781 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     6841 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     6901 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     6961 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     7021 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     7081 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     7141 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     7201 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     7261 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     7321 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     7381 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     7441 cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     7501 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     7561 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     7621 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     7681 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     7741 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     7801 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     7861 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     7921 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     7981 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     8041 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     8101 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagcatt
     8161 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     8221 tttctcgttc agctttcttg tacaaagtgg tgcctagggc ggccgcggtc ctgcaacttg
     8281 aggtagtcaa gatgcataat aaataacgga ttgtgtccgt aatcacacgt ggtgcgtacg
     8341 ataacgcata gtgtttttcc ctccacttaa atcgaagggt tgtgtcttgg atcgcgcggg
     8401 tcaaatgtat atggttcata tacatccgca ggcacgtaat aaagcgaggg gttcgaatcc
     8461 ccccgttacc cccggtaggg gcccaggtac ccggatgtgt tttccgggct gatgagtccg
     8521 tgaggacgaa acctggctgc aggcatgcaa gcttggcgta atcatggtca tagcctagag
     8581 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
     8641 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
     8701 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     8761 ttctaattcc taaaaccaaa atccagtgac ctgcagcccg gccgggggat ccactagcag
     8821 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
     8881 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     8941 ttatccgttc gtccatttgt atgtgcatgc caaccacagg agatctcagt aaagcgctgg
     9001 ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat
     9061 cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga
     9121 cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt
     9181 ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg
     9241 tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca
     9301 gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca
     9361 ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga
     9421 agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc
     9481 cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc
     9541 cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg
     9601 cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct
     9661 tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg
     9721 ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa
     9781 gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc
     9841 tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc
     9901 gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac
     9961 gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct
    10021 cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg
    10081 cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga
    10141 tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga
    10201 ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct
    10261 tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt
    10321 ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca
    10381 aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc
    10441 gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa ttcatccagt
    10501 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
    10561 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
    10621 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
    10681 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
    10741 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
    10801 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
    10861 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    10921 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    10981 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    11041 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    11101 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    11161 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    11221 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    11281 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    11341 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
    11401 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac
    11461 aattatgggt gatgctgcca actcgagagc gggccgggag ggttcgagaa gggggggcac
    11521 cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta
    11581 taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga
    11641 aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg
    11701 cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag
    11761 tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca
    11821 tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc
    11881 acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg
    11941 cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt
    12001 atccacaacg ccggcggccg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
    12061 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
    12121 gtctagtgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
    12181 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
    12241 caacttaata acacattgcg gacgttttta atgtactggg gtggttttgg taccgggccc
    12301 cccctcgagg tcgacggtat cgataagctt gatatcgaat t
//
