LOCUS       40924_40517        5607 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pSK(+)PICT1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 5607)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..546
                     /label=Ulva RbcS promoter
                     /note="Ulva RbcS promoter"
                     /regulatory_class="promoter"
     repeat_region   558..668
                     /label=derived from Escherichia coli transposon Tn5
                     /note="derived from Escherichia coli transposon Tn5"
     intron          680..842
                     /number=1
                     /note="derived from Ulva RbcS"
     CDS             complement(990..1646)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1647..1749)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     intron          1843..2175
                     /number=1
                     /note="derived from Ulva RbcS"
     repeat_region   2182..2293
                     /label=derived from Escherichia coli transposon Tn5
                     /note="derived from Escherichia coli transposon Tn5"
     regulatory      2301..2689
                     /label=Ulva RbcS terminator
                     /note="Ulva RbcS terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2705..2721)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2747..2765)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2772..2788)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2933..3388
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3417..3521
                     /label=AmpR promoter
     CDS             3522..4379
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4553..5141
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5429..5450
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5465..5495
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5503..5519
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5527..5543
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5564..5582
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gctatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacatgg ccaaggggat ccaaactgga tggctttctt gcccgccaag gatctgatgg
      601 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
      661 gatggattac tagtgccaag tgcgcatcgc gtgtcaatgc tggcgctgct gacgtccgct
      721 gcctaaactg catgaaagct cctgcagtcg caagtagtcc ttttaacttg ttgtatcgga
      781 acaaatggtt gtagtctgtg ccgtgtgacg atcttgagcc gcctggtcgg caggtccaat
      841 gtacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
      901 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa
      961 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
     1021 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     1081 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     1141 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     1201 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     1261 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     1321 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     1381 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
     1441 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     1501 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     1561 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     1621 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     1681 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     1741 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     1801 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggcctgga ccctgcacac
     1861 cctgcccacc ctgccgctgc tcacccgttc acgaccccac aggtgctcgc agcagtaatt
     1921 gtcgatagat agcaactgtg ttctgactat tctttgacct tccgcatttg ggactgcggc
     1981 ggcacaccta ctatccgcaa tgcacaccct ggtgctggta ctgcctgcct ttccgcgcga
     2041 gcccacacca tgcaggcgca tagtaatgga ctgtgattga tttaggcaac tgtatgaaac
     2101 ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg agttcatatg accaacgctg
     2161 gcacgacccc tgcaggatca actagtaatc catcttgttc aatcatgcga aacgatcctc
     2221 atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc gggcaagaaa
     2281 gccatccagt ttggatcccc gcagtcaggc agagtcgttg ttgggtgtat ggttgcacaa
     2341 aactagacct cgagctggtc ggttagaaag agatgcttgt gcgactggat gtaacaggca
     2401 caatccgagg aaaatgcaac agaaacgtta tgatgtaaca tctgagcaca gtcaataaaa
     2461 ttgcatgttg tatgccgcac acaaaagtgt tgttaaacat gcgatgtggt tgtcaaatca
     2521 ttattcgcac aaccatggac cgaacctctg gcacgctgca tacgctgtat tcattcacat
     2581 catgttttct acttgtcaca tgctctgcac caagtaactc cgattagtta ggttcgcaca
     2641 tgtcatcgga gtggaccact tcgaatcatt tggttcgaag acaaaccggg gcatgcaagc
     2701 ttatcgatac cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg
     2761 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2821 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2881 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc
     2941 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3001 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3061 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3121 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3181 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3241 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3301 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3361 tttaacaaaa tattaacgtt tacaatttcc caggtggcac ttttcgggga aatgtgcgcg
     3421 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3481 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3541 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3601 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3661 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3721 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3781 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3841 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3901 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3961 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4021 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4081 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4141 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4201 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4261 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4321 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4381 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4441 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4501 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4561 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4621 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4681 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4741 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4801 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4861 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4921 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4981 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5041 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5161 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5221 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5281 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5341 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5401 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5461 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5521 ttcacacagg aaacagctat gaccatgatt acgccaagct cggaattaac cctcactaaa
     5581 gggaacaaaa gctggagctc ggtaccc
//
