LOCUS       40924_40507        5614 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Rosalind Kim, Steve J. Sandler, Stanley Goldman, Hisao Yokota, Alvin
            J. Clark, Sung-Hou Kim
  TITLE     Overexpression of archaeal proteins in Escherichia coli
  JOURNAL   Biotechnology Letters. March 1998. 20(3): 207-210.
REFERENCE   2  (bases 1 to 5614)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5614)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechnology Letters. March 1998. 20(3): 207-210."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     663..680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1072..1174
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1175..1831
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(3673..4461)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     tRNA            complement(4981..5057)
                     /label=argU
     tRNA            5304..5379
                     /label=ileX
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta
      841 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
      901 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact
      961 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
     1021 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
     1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1381 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1441 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1501 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1561 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1621 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1681 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1741 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     1801 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     1861 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     1921 gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc
     1981 tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat
     2041 gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcatt
     2101 tattctgcct cccagagcct gataaaaacg gttagcgctt cgttaataca gatgtaggtg
     2161 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg
     2221 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgctgccg
     2281 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     2341 tgccccgcgc ccaccggaag gagctaccgg cagcggtgcg gactgttgta actcagaata
     2401 agaaatgagg ccgctcatgg cgttgactct cagtcatagt atcgtggtat caccggttgg
     2461 ttccactctc tgttgcgggc aacttcagca gcacgtaggg gacttccgcg tttccagact
     2521 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     2581 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     2641 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     2701 ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
     2761 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
     2821 aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg
     2881 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct
     2941 acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc
     3001 agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc
     3061 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     3121 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     3181 aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     3241 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     3301 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     3361 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     3421 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     3481 ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     3541 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcgagctcga cccgcatagt
     3601 cttccccagc tctctaacgc ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg
     3661 aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtgaacaaa
     3721 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat aagcctgcct
     3781 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     3841 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     3901 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     3961 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     4021 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     4081 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
     4141 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     4201 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
     4261 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
     4321 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
     4381 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
     4441 ggcgatcacc gcttccctca tgatgtttaa ctcctgaatt aagccgcgcc gcgaagcggt
     4501 gtcggcttga atgaattgtt aggcgtcatc ctgtgctccc gagaaccagt accagtacat
     4561 cgctgtttcg ttcgagactt gaggtctagt tttatacgtg aacaggtcaa tgccgccgag
     4621 agtaaagcca cattttgcgt acaaattgca ggcaggtaca ttgttcgttt gtgtctctaa
     4681 tcgtatgcca aggagctgtc tgcttagtgc ccactttttc gcaaattcga tgagactgtg
     4741 cgcgactcct ttgcctcggt gcgtgtgcga cacaacaatg tgttcgatag aggctagatc
     4801 gttccatgtt gagttgagtt caatcttccc gacaagctct tggtcgatga atgcgccata
     4861 gcaagcaggt cgagctcgca tgcgaggaaa atagacgaca tttttggtga taatgtccca
     4921 aatatgtccc actctgaaat tatggaggat ataaagaagg cgtaactgat tgaattgtaa
     4981 tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc
     5041 aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat tatacggtca
     5101 acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga ccgcgtctga
     5161 ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg tctgaatcaa
     5221 gaaaacccgt attttcatgt atcaaagtga cctgcagcca agcttggatt gcgacacgga
     5281 gttactttat aatccaatcg attggcccct tagctcagtg gttagagcag gcgactcata
     5341 atcgcttggt cgctggttca agtccagcag gggccaccag cggccgcaaa ggctgacgag
     5401 aaatcgtcag cctttttaag ctttaagccg aattccagca cactggcggc cgttactagt
     5461 ggatccgagc tcggtaccaa gcttatcgat gataagctgt caaacatgag aattacaact
     5521 tatatcgtat ggggctgact tcaggtgcta catttgaaga gataaattgc actgaaatct
     5581 agaaatattt tatctgatta ataagatgat cttc
//
