LOCUS       40924_40497        3664 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI_DAZ-DM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 3664)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        71..374
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        376..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        615..724
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    830..851
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        866..896
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    904..920
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     928..944
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        962..980
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1173..1220
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1330..1339
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1370..1388)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1395..1411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1700..1924
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2066..2170
                     /label=AmpR promoter
     CDS             2171..3028
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(3203..3584)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(3626..3659)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttggcgcgcc tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
       61 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      121 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      181 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      241 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      301 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
      361 tcgctattac catgctgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
      421 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      481 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
      541 gtaggcgtgt acggtgggag gtctatataa gcagacgtcg tttagtgaac cgtcagatca
      601 ccatccagta tacggacctt taattcaacc caacacaata tattatagtt aaataagaat
      661 tattatcaaa tcatttgtat attaattaaa atactatact gtaaattaca ttttatttac
      721 aatcactcga cgaagacttg atcacccggg atctcgaaag cttggtaccg agctcaaagg
      781 agatatacca tgggaagagc tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      841 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      901 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      961 tatttaggtg acactataga atactcaagc tatgcatcca acgcgttggg agctctccca
     1021 tatggtcgac ctgcaggcgg ccgcactagt gattgatcgt cgacggatga tcctccagcg
     1081 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     1141 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     1201 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     1261 taccccaccg agaccccatt ggggccaata cgcccgcgta gggataacag ggtaatgata
     1321 atcccgcggc catggcggcc gggagcatgc gacgtcgggc ccaattcgcc ctatagtgag
     1381 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1441 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1501 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gtgatcaccc
     1561 gggatctcga gccatggtgc tagcagctga tgcatagcat gcggtaccgg gagatggggg
     1621 aggctaactg aaacacggaa gggctcttcc tgaccgctcg agtctagagg gcccgtttaa
     1681 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     1741 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     1801 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     1861 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     1921 atggcttctg aggcggaaag aaccagctgg ggctctaggt taattaagat cgatccctag
     1981 gctttacgta actataacgg tcctaaggta gcgaataccg tcgacctcta gccctgcagg
     2041 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2101 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2161 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2221 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2281 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2341 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2401 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2461 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2521 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2581 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2641 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2701 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2761 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2821 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2881 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2941 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3001 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3061 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3121 taatctcatg accaaaatcc cttaacgtga gtttaaagaa catgtgagca cctgagtgct
     3181 tgcggcagcg tgaagctaat tctgtcagcc gttaagtgtt cctgtgtcac tcaaaattgc
     3241 tttgagaggc tctaagggct tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt
     3301 taaaccttaa aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc
     3361 ttagaggcta tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag
     3421 tacgtgaaac atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg
     3481 gtttagttcg ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac
     3541 atgagagctt agtacgtact atcaacaggt tgaactgctg atcgatcttt tctacggggc
     3601 ggccgcgtca gtcagtcatc ataccataac ttcgtatagc atacattata cgaagttatc
     3661 tggt
//
