LOCUS       Exported                7435 bp DNA     circular SYN 21-APR-2025
DEFINITION  Exported.
ACCESSION   V011282
VERSION     .
KEYWORDS    pSIP403
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Sorvig E, Mathiesen G, Naterstad K, Eijsink VG, Axelsson L
  TITLE     High-level, inducible gene expression in Lactobacillus sakei and 
            Lactobacillus plantarum using versatile expression vectors.
  JOURNAL   Microbiology. 2005 Jul;151(Pt 7):2439-49. doi: 
            10.1099/mic.0.28084-0.
  PUBMED    16000734
REFERENCE   2  (bases 1 to 7435)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7435)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7435)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2005-07"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(542..7435,1..541)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..672
                     /codon_start=1
                     /product="Encodes a response regulator. Receives the 
                     phosphate group from phosphorylated sppK, then binds to DNA
                     to regulate gene transcription."
                     /label=SppR
                     /translation="MINEFAMTLTCAASDTETLLAAIKDQQRGLFFLDMEIEDNRQAGL
                     EVATKIRQMMPFAQIVFITTHEELTLLTLERKIAPLDYILKDQTMAEIKRQLIDDLLLA
                     EKQNEAAAYHRENLFSYKIGPRFFSLPLKEVVYLYTEKENPGHINLLAVTRKVTFPGNL
                     NALEAQYPMLFRCDKSYLVNLSNIANYDSKTRSLKFVDGSEAKVSFRKSRELVAKLKQM
                     M"
     promoter        972..1115
                     /label=PsppA
                     /note="A DNA sequence upstream of the sppA gene. Inactive 
                     without an inducing signal; activates gene transcription 
                     when stimulated."
     CDS             1116..2915
                     /label=GUS
                     /note="beta-glucuronidase"
     rep_origin      3272..3860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4032..4719
                     /label=256rep
                     /note="p256 replication region; allows replication in 
                     Lactobacillus species"
     CDS             5049..5783
                     /gene="ermBP"
                     /label=rRNA adenine N-6-methyltransferase
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             6013..7359
                     /codon_start=1
                     /product="Encodes a histidine-kinase in a two-component 
                     system. It senses extracellular signals (like pheromones, 
                     IP-673) and autophosphorylates upon signal binding."
                     /label=SppK
                     /translation="MLYTDVSVSLMQNFVAILLIFLLYRYIQRKITFKRIILDILIAII
                     FSILYLFISDASLLVMVLMRLGWHFHQQKENKIKTTDTANLILIIVIQLLLVAVGTIIS
                     QFTISIIKSDFSQNILNNSATDITLLGIFFAVLFDGLFFILLKNKRTELQHLNQEIIEF
                     SLEKQYFIFIFILFIVIEIILAVGNLQGVTATILLTIIIIFCVLIGMTFWQVMLFLKAY
                     SIRQEANDQLVRNQQLQDYLVNIEQQYTELRRFKHDYQNILLSLESFAEKGDQQQFKAY
                     YQELLAQRPIQSEIQGAVIAQLDYLKNDPIRGLVIQKFLAAKQAGVTLKFEMTEPIELA
                     TANLLTVIRIIGILLDNAIEQAVQETDQLVSCAFLQSDGLIEITIENTASQVKNLQAFS
                     ELGYSTKGAGRGTGLANVQDLIAKQTNLFLETQIENRKLRQTLMITEET"
ORIGIN
        1 atgattaacg aatttgcaat gactttaaca tgcgctgcca gtgatactga gacattgttg
       61 gcggcaatta aggatcagca acgaggttta ttctttttgg atatggaaat tgaggataac
      121 cgccaagccg gtttagaagt ggcaactaag attcggcaga tgatgccgtt tgcgcaaatt
      181 gtcttcatta caacccacga ggaactgaca ttattaacgt tagaacgaaa aatagcgcct
      241 ttagattaca ttctcaagga ccaaacaatg gctgaaatca aaaggcaatt gattgatgat
      301 ctattgttag ctgagaagca aaacgaggcg gcagcgtatc accgagaaaa tttatttagt
      361 tataaaatag gtcctcgctt tttctcatta ccattaaagg aagttgttta tttatatact
      421 gaaaaagaaa atccgggtca tattaatttg ttagccgtta ccagaaaggt tacttttcca
      481 ggaaatttaa atgcgctgga agcccaatat ccaatgctct ttcggtgtga taaaagttac
      541 ttagttaacc tatctaatat tgccaattat gacagtaaaa cacggagttt aaaatttgta
      601 gatggcagtg aggcaaaagt ctcgttccgg aaatcacggg aactagtggc caaattaaaa
      661 caaatgatgt agcgcctgca gcacgccaaa tgatcccagt aaaaagccac ccgcatggcg
      721 ggtggctttt tattagccct agaagggctt cccacacgca tttcagcgcc ttagtgcctt
      781 agtttgtgaa tcataggtgg tatagtcccg aaatacccgt ctaaggaatt gtcagatagg
      841 cctaatgact ggcttttata atatgagata atgccgactg tactttttac agtcggtttt
      901 ctaatgtcac taacctgccc cgttagttga agaaggtttt tatattacag ctccagatct
      961 accggtgggc ccatattaac gtttaaccga taaagttgaa cgttaatatt ttttttgcgc
     1021 agaaatggta aattgaagca taatagtctt gtaaggtatt tagctggctg gcgtaaagta
     1081 tgctttataa aataatatat aggagtatga ttcccatggt acgtcctgta gaaaccccaa
     1141 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
     1201 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
     1261 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
     1321 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
     1381 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
     1441 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
     1501 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
     1561 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
     1621 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
     1681 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
     1741 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
     1801 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
     1861 tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg
     1921 tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct
     1981 actttactgg ctttggtcgt catgaagatg cggacttgcg tggcaaagga ttcgataacg
     2041 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     2101 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     2161 ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca
     2221 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     2281 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     2341 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     2401 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     2461 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     2521 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     2581 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     2641 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     2701 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     2761 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     2821 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     2881 ctggcatgaa cttcggtgaa aaaccgcagg gaggcaaaca atgatctaga ctcgaggaat
     2941 tcggtacccc gggttcgaag gcgccaagct tcaaattaca gcacgtgttg ctttgattga
     3001 tagccaaaaa gcagcagttg ataaagcaat tactgatatt gctgaaaaat tgtaatttat
     3061 aaataaaaat caccttttag aggtggtttt tttatttata aattattcgt ttgatttcgc
     3121 tttcgataga acaatcaaag cgagaataag gaagataaat cccataaggg cgggagcaga
     3181 atgtccgaga ctaattcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3241 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3301 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3361 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3421 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3481 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3541 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3601 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3661 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3721 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3781 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3841 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3901 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3961 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgctgcgagt
     4021 cagtgagcga gaaggattat tcggctggtt gagacgttaa aatgataaag gttgtattaa
     4081 tcttatatta cggttataat gtactcaact taataaatga acgcaaaaaa aagaaccctc
     4141 aacttagcag agttaggatt cacgacttat cagcacaacc tgataagatt ttcgatagca
     4201 agtactacca atacaagcta tctaacttgg tactattata acatgtaggc taagtttttc
     4261 aaccattgat acttaaagta aacggttgtt atcgggaatc ttaacagaaa cctgatagca
     4321 accgtttttt tgttattcaa tggttagcaa ccatcaaagc aactaaaggc tggaaacctg
     4381 ttcttagcta gtaaaacctc ccgtgagtgt cgttcgtgac cccgcttgca gttaacaaca
     4441 taggtatgct aaaccttgtc gagatcaacg cgactaaaga cgtggctgga agactaggaa
     4501 atgatacgga caggctaact attaacgcag attattcggg ttgctgctaa aaccaactct
     4561 aataatagtt agtgcaaggg ctggttgagc ttaaattgtc tgataaagag ttctctcttt
     4621 atactgcaaa agaagcgcag ttattcacga ttaggataac tgtttgagag agcctaaggg
     4681 cttgaccctt gatggtttaa gcaccgctat gcgtgcggga tccccttaga agcaaactta
     4741 gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa gttaaattag
     4801 atgctaaaaa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
     4861 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
     4921 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
     4981 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttcagga
     5041 gagattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac
     5101 tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa
     5161 caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg
     5221 aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaat actcgtgtca
     5281 ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg
     5341 ttgggaatat tccttacaat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc
     5401 gtgcgtctga catctatctg actgttgaag aaggattcta caagcgtacc ttggatattc
     5461 accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc
     5521 cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc
     5581 ataccacaga tgttccagat aaatattgga agctatataa gtactttgtt tcaaaatggg
     5641 tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcgtcaagca atgaaacacg
     5701 ccaaagtaaa caatttaagt accattactt atgagcaagt attgtctatt tttaatagtt
     5761 atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt tggaaagtta
     5821 cacgttacta aagggaatgg agaccggggt cgacttcaat agagttctta acgttaatcc
     5881 gaaaaaaact aacgttaata ttaaaaaata agatccgctt gtgaattatg tataatttga
     5941 ttagactaaa gaataggaga aagtatgatg atatttaaaa aactttctcg ttaagatagg
     6001 ttgttggtga gcatgttata tacggatgta tcggtttcct taatgcaaaa ttttgttgct
     6061 atcttattaa tttttctatt atatagatat attcaaagaa agataacatt taaacggatc
     6121 atattagata ttttaatagc gattattttt tcaatattat atctgtttat ttcagatgcg
     6181 tcattacttg taatggtatt aatgcgatta gggtggcatt ttcatcaaca aaaagaaaat
     6241 aagataaaaa cgactgatac agctaattta attctaatta tcgtgatcca gttattgtta
     6301 gttgcggttg ggactattat tagtcagttt accatatcga ttatcaaaag tgatttcagc
     6361 caaaatatat tgaacaatag tgcaacagat ataactttat taggtatttt ctttgctgtt
     6421 ttatttgacg gcttgttctt tatattattg aagaataagc ggactgaatt acaacattta
     6481 aatcaagaaa tcattgaatt ttcgttagaa aaacaatatt ttatatttat atttatttta
     6541 tttatagtaa tagaaattat tttagcagtt gggaatcttc aaggagtaac agccacgata
     6601 ttattaacca ttatcattat tttttgtgtc cttatcggga tgactttttg gcaagtgatg
     6661 ctttttttga aggcttattc gattcgccaa gaagccaatg accaattggt ccggaatcaa
     6721 caacttcaag attatctagt caatatcgaa cagcagtaca ccgaattacg gcgatttaag
     6781 catgattatc aaaacatctt attatcgttg gagagttttg ccgaaaaggg cgatcagcaa
     6841 cagtttaagg cgtattacca agaattatta gcacaacggc caattcaaag tgaaatccaa
     6901 ggggcagtca ttgcacaact cgactacttg aaaaatgatc ctattcgagg attagtcatt
     6961 caaaagtttt tggcagccaa acaggctggt gttactttaa aattcgaaat gaccgaacca
     7021 atcgaattag caaccgctaa tctattaacg gttattcgga ttatcggtat tttattagac
     7081 aatgcgattg aacaagccgt tcaagaaacc gatcaattgg tgagttgtgc tttcttacaa
     7141 tctgatggtt taatcgaaat tacgattgaa aatacggcca gtcaagttaa gaatctccaa
     7201 gcattttcag agttaggcta ttcaacgaaa ggcgctggtc gggggactgg tttagctaat
     7261 gtgcaggatt tgattgccaa acaaaccaat ttattcttag aaacacagat tgaaaataga
     7321 aagttacgac agacattgat gattacggag gaaacttaat ttgtatcccg tttatttatt
     7381 agaggatgat ttacagcaac aagcgattta tcagcaaatt atcgcgaata cgatt
//
