LOCUS       Exported                8388 bp DNA     circular SYN 20-JUL-2025
DEFINITION  Exported.
ACCESSION   V017137
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8388)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2096..2115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1193..1900
                     /label=PAmCherry1
                     /note="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
     misc_feature    2083..2681
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2701..3297
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     LTR             3503..3736
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3893..4117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4213..4317
                     /label=AmpR promoter
     CDS             4318..5175
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5349..5937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     LTR             6096..6729
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6776..6901
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    7394..7627
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7812..7856
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             8005..8046
                     /label=Protein Tat
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    8143..8260
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        8388
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattcgcca ccatggtgag
     1201 caagggcgag gaggataaca tggccatcat taaggagttc atgcgcttca aggtgcacat
     1261 ggaggggtcc gtgaacggcc acgtgttcga gatcgagggc gagggcgagg gccgccccta
     1321 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcacctg
     1381 ggacatcctg agccctcagt tcatgtacgg ctccaatgcc tacgtgaagc accccgccga
     1441 catccccgac tactttaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     1501 attcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     1561 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg tgatgcagaa
     1621 gaagaccatg ggctgggagg ccctctccga gcggatgtac cccgaggacg gcgccctgaa
     1681 gggcgaggtc aagcccagag tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     1741 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaaccgcaa
     1801 gctggacatc accagccaca acgaggacta caccatcgtg gagcagtacg agagagccga
     1861 gggccgccac tccaccggcg gcatggacga gctgtacaag gctagcaagg aatcccgggc
     1921 caagaaattc cagcggcagc atatggactc agacagttcc cccagcacca aagaaaaccc
     1981 tcgtagcaac caggaagaaa gctatgatga taacgaaagc taagtttaaa caatcactag
     2041 ttcgaaggat ccgcatgcat ctagggcggc caattccgcc cctctccccc cccccccctc
     2101 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     2161 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     2221 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     2281 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     2341 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     2401 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     2461 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     2521 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     2581 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     2641 ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag acgaccttcc
     2701 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     2761 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     2821 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     2881 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     2941 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     3001 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     3061 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     3121 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     3181 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     3241 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     3301 taataggcgg ccgctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     3361 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     3421 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     3481 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     3541 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3601 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3661 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     3721 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     3781 aagaaatgaa tatcagagag tgagaggcct tgacattata atagatttag caggaattga
     3841 actaggagtg gagcacacag gcaaagttct agagctcgct gatcagcctc gactgtgcct
     3901 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3961 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4021 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4081 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4141 tgggggcgcg cccctcgagg ccgccatggt catagctgtt tgacgtcagg tggcactttt
     4201 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4261 ccgctcacga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4321 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4381 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4441 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4501 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4561 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4621 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4681 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4741 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4801 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4861 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4921 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4981 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5041 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5101 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5161 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5221 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5281 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5461 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5941 gccagcaacg cggccttttt acggttctgg ccttttgcac ggaccgccac tgccaattac
     6001 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     6061 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     6121 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     6181 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     6241 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     6301 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     6361 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     6421 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     6481 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     6541 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6601 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6661 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6721 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     6781 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     6841 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     6901 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     6961 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     7021 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     7081 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     7141 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     7201 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     7261 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     7321 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     7381 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     7441 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     7501 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     7561 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     7621 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     7681 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     7741 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     7801 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     7861 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     7921 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     7981 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     8041 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     8101 gatctcgacg gtatcgccac aaatggcagt attcatccac aattttaaaa gaaagggggg
     8161 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     8221 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     8281 cagagatcca ctttggatcg ataagctttg caaagatgga taaagtttta aacagagagg
     8341 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
