LOCUS       V014835                 8517 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014835
VERSION     V014835
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1415..1648
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1833..1877
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2026..2067
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2164..2281
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2410..3588
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3608..4339
                     /label="mNeptune2"
                     /note="far-red fluorescent protein with spectra similar to
                     those of mNeptune but improved brightness"
     misc_feature    4437..5023
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5043..5834
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     LTR             6040..6273
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6430..6656
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6752..6856
                     /label="AmpR promoter"
     CDS             6857..7714
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7888..8475
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggatc gataagcttt gcaaagatgg
     2341 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     2401 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2461 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2521 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2581 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     2641 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     2701 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     2761 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     2821 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     2881 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     2941 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     3001 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     3061 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     3121 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     3181 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     3241 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     3301 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     3361 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     3421 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     3481 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     3541 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattgggatc
     3601 cgccaccatg gtgtctaagg gcgaagagct gattaaggag aacatgcaca tgaagctgta
     3661 catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc
     3721 ctacgagggc acccagaccg gcagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     3781 cttcgacatc ctggctacct gcttcatgta cggcagcaag accttcatca accacaccca
     3841 gggcatcccc gatttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac
     3901 cacatacgaa gacgggggcg tgcttaccgt tacccaggac accagcctcc aggacggctg
     3961 cttgatctac aacgtcaagc tcagaggggt gaacttccca tccaacggcc ctgtgatgca
     4021 gaagaaaaca ctcggctggg aggccagtac cgagaccctg taccccgctg acggcggcct
     4081 ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg ggccacctgc actgcaacct
     4141 gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctactt
     4201 tgtggaccgc agactggaaa gaatcaagga ggccgacaat gagacctacg tcgagcagca
     4261 cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaatgg
     4321 catggacgag ctgtacaagt aaggatcccc ggacgaattc ttcgaacata tgaagcttgc
     4381 tagcactagt gatccgcatg catctagggc ggccaattcc gcccctctcc cccccccccc
     4441 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     4501 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     4561 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     4621 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     4681 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     4741 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     4801 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     4861 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     4921 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     4981 gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt
     5041 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5101 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     5161 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     5221 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     5281 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     5341 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     5401 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     5461 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     5521 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     5581 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     5641 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5701 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5761 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5821 ttgacgagtt cttctgataa taggcggccg ctcgagacct agaaaaacat ggagcaatca
     5881 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     5941 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     6001 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6061 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     6121 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6181 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6241 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     6301 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattataata
     6361 gatttagcag gaattgaact aggagtggag cacacaggca aagttctaga gctcgctgat
     6421 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6481 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6541 cgcattgtct gagtaggtgt cattctattc tggggggtgg gggtggggca ggacagcaag
     6601 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     6661 tgaggcggaa agaaccagct gggggcgcgc ccctcgaggc cgccatggtc atagctgttt
     6721 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6781 aatacattca aatatgtatc cgctcacgag acaataaccc tgataaatgc ttcaataata
     6841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6961 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7021 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7081 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7141 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7201 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7261 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7321 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7381 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7441 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7501 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7561 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7621 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7681 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7741 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8161 ggactcaaga cgatagttac cggataagcg cagcggtcgg gctgaacggg gggttcgtgc
     8221 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8281 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8341 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8401 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8461 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttctggcc ttttgca
//
