LOCUS       62056_19755        6972 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1780..2637
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     oriT            complement(2771..2880)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      3051..3439
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     primer_bind     3472..3488
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  complement(3857..4624)
                     /label=IS1
                     /note="prokaryotic transposable element"
     protein_bind    5416..5434
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(6488..6506)
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(6858..6972,1..506)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline repressor TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
ORIGIN
        1 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga
       61 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt
      121 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag
      181 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct
      241 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc
      301 attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg
      361 aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc
      421 accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa
      481 aacaacttaa atgtgaaagt gggtcttaag gtaccgagga cgcgtaaaca tttacagttg
      541 catgtggcct attgttttta gccgttaaat attttataac tattaaatag cgatacaaat
      601 tgttcgaaac taatattgtt tatatcatat attctcgcat gttttaaagc tttattaaat
      661 tgattttttg taaacagttt ttcgtactct ttgttaaccc atttcattac aaaagtttca
      721 tatttttttc tctctttaaa tgccattttt gctggctttc tttttaatac aattaatgtg
      781 ctatccactt taggttttgg atggaaataa tacctaggaa tttttgctaa tatagaaata
      841 tctacctctg ccattaacag caatgctagt gatctgtttg tatctaataa cattttagca
      901 aaaccatatt ccactattaa ataacttatt gtggctgaac tttcaaaaac aatttttcga
      961 attatatttg tgcttatgtt gtaaggtatg ctgccaaata ttttatatgg attgtggcta
     1021 ggaaatgtaa atttcagtat atcatcattt actatttgat agttaggata atttaagagc
     1081 ttattacgag ttacctcaca taatttagaa tcaatttcta tcgccgttac aaaattacat
     1141 ctctttacca atccagcagt aaaatgacct ttccctgcac ctatttcaaa gatgttatct
     1201 ttttcatcta aacttatgca attcattatt ttttctatgt gatattttga agtaataaaa
     1261 ttttgactat cttttatatt tactttgttc attataacct ctccttaatt tattgcatct
     1321 cttttcgaat atttatgttt tttgagaaaa gaacgtactc atggttcatc ccgatatgcg
     1381 tatcggtctg tatatcagca actttctatg tgtttcaact acaatagtca tctattctca
     1441 tctttctgag tccaccccct gcaaagcccc tctttacgac ataaaaattc ggtcggaaaa
     1501 ggtatgcaaa agatgtttct ctctttaaga gaaactcttc gggatgcaaa aatatgaaaa
     1561 taactccaat tcaccaaatt atatagcgac ttttttacaa aatgctaaaa tttgttgatt
     1621 tccgtcaagc aattgttgag caaaaatgtc ttttacgata aaatgatacc tcaatatcaa
     1681 ctgtttagca aaacgatatt tctcttaaag agagaaacac ctttttgttc accaatcccc
     1741 gacttttaat cccgcggcca tgattgaaaa aggaagagta tgagtattca acatttccgt
     1801 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1861 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1921 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1981 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2041 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2101 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2161 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2221 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2281 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2341 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2401 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2461 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2521 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2581 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2641 ctgtcagacc aagtttactc ataacgcgtc aattcgaggg ggatcaattc cgtgataggt
     2701 gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg
     2761 ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
     2821 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
     2881 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
     2941 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
     3001 tatgcagcgg aaaacggaat tgatccggcc acgatgcgtc cggcgtagag gatctgaaga
     3061 tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct
     3121 catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta
     3181 agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa
     3241 caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga
     3301 aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc
     3361 cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg agtgacacag
     3421 gaacacttaa cggctgacat gggaattccc ctccaccgcg gtggcggccg ctctagaact
     3481 agtggatcct cataatgcta atcttacacc gtcccaccac atttccaatt cagaagctct
     3541 attgtctatc cagtcataga actcgtcacc ccaagtataa tcagccactc cgataccgat
     3601 agctaagacc caaccgacat agggaataaa agtaacgcca taagctaaac ctgctacggc
     3661 aattcttgta cctgccacgg caaatcgacc attttgcaca tcacgcacaa cttgatagcc
     3721 agtaagcacc attcctgccc atcccaaagc tttacctact ccacgcactc tactcatacc
     3781 tttagcgggg acttgatagt tccctaaagg caatcttata atcggcggaa gcgttttggg
     3841 cgagaaacgc atctgcggta atgactccaa cttattgata gtgttttatg ttcagataat
     3901 gcccgatgac tttgtcatgc agctccaccg attttgagaa cgacagcgac ttccgtccca
     3961 gccgtgccag gtgctgcctc agattcaggt tatgccgctc aattcgctgc gtatatcgct
     4021 tgctgattac gtgcagcttt cccttcaggc gggattcata cagcggccag ccatccgtca
     4081 tccatatcac cacgtcaaag ggtgacagca ggctcataag acgccccagc gtcgccatag
     4141 tgcgttcacc gaatacgtgc gcaacaaccg tcttccggag actgtcatac gcgtaaaaca
     4201 gccagcgctg gcgcgattta gccccgacat agccccactg ttcgtccatt tccgcgcaga
     4261 cgatgacgtc actgcccggc tgtatgcgcg aggttaccga ctgcggcctg agttttttaa
     4321 gtgacgtaaa atcgtgttga ggccaacgcc cataatgcgg gctgttgccc ggcatccaac
     4381 gccattcatg gccatatcaa tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt
     4441 gaactgcagt tgccatgttt tacggcagtg agagcagaga tagcgctgat gtccggcggt
     4501 gcttttgccg ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagaaac
     4561 agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat
     4621 cacccgcatc tgcttaggta agtacgcacc tcgattatcc ataatcttct cagcccaatt
     4681 tccggctgtt tcagcaattc cgaatcctaa ccctgccagt ccttccagat tgggtccttc
     4741 cttatttaca gcaggcaggc taccagaatc gcgtaccgct gacaccaagt ccgtcaaact
     4801 ctcatacttt gcctcataag tctttccatt tgagttatcc gtggcactaa tcacaatttc
     4861 gtaccgactg ccagtgtcag taattacaat acttatctga ctattatccg tgggcaaata
     4921 ttgcttttct tggcctaaat ttatctcagt gatattatta cctttgtttt taactattaa
     4981 gtcacggacc ttttcggaaa cttttgttgg atcttgcatc agactttttt cataggcata
     5041 ttgggaggta tcgtatttcc tttctgagac tatcgtatga aaactctcgt ccaccgaaac
     5101 attccgagat attggactcc atccgttttg atttaccggt agaattacct tgctactcat
     5161 ccaagtatat cccaagccaa ctacaaatcc ttcatagtca tccccccgtt ccaagatgac
     5221 attaaattca ctttgcgttt tatattcatt tctataagcg ccggcaaatg agaactgtat
     5281 agctacaata gccatcacaa gcaaagataa aattttcttc atggtgtctt ttcttttata
     5341 tgtctttatt tcgtctctat cactgatagg gagactgcaa agataagaac ttttagcttt
     5401 atcttccaaa ctttatccct atcagtgata gagatttttt tgttcttatg gtcaagcagt
     5461 actgcgcttt tacactaagt caatttaaca aggctttctt ctcttggaaa gcgggtgcaa
     5521 aaactgcagt tagaatttaa ctcctagatt acctaaattt ttttgcatct ctatataatg
     5581 tgaattcagt attttgtgac attcattcat cttttcctct acagatttgt ctccactttc
     5641 aaattttttt tgtaattctc gatattttat tagtgttttt ttgtaatcgc tataatttat
     5701 acatgctaat ctccattgca tcaagtccgt ctcatacatc catgtgccta aacttatgac
     5761 agtcatatag ggacttaatt ttgtttttcc ggctgcaaac agtaaaatgt ctccggcagc
     5821 tgaccaatat tcaccattac ctacctcatc tataatagaa ataagcgaca atccatctcc
     5881 aattttgcct atatgtttat tatccattag catattcgct gcatcaaggg gagctccgat
     5941 ttggtcattt atttgactac ctaatgatag tatcccattt cttgcgtcat ttccctttag
     6001 tgctcctttg gattttatta tgggaagagg tatttcacaa taacttttaa tagcatccgc
     6061 ccgatgaata aattttagca tctcctccac gataccataa aatgcataat aggcatcgta
     6121 atccaatttc aacagttcat cttttgacaa gacatggagt gtatgtaatg ttttggtctc
     6181 aatgattgca tctttccttt gaaaataggg gaaaggtatt tgctgatatt ttggaatcgc
     6241 ccataattgt tctggattag tgcggataaa atatagggtt tctgttgtcc aatacacata
     6301 cccgatgcct tctaccaatc ccgtataggt cgtattaccg gcattcacca tagcctcgaa
     6361 ctctggcaat ttctttattt ctatttcctt accgccggca aatgagaact gtatagctac
     6421 aatagccatc acaagcaaag ataaaatttt cttcatggtg tcttttcttt tatatgtctt
     6481 tatttcgtct ctatcactga tagggagact gcaaagataa gaacttttag ctttatcttc
     6541 caaactttat ccctatcagt gatagagatt tttttgttct tatggtcaag cagtactgcg
     6601 cttttacact aagtcaattt aacaaggctt tcttctcttg gaaagcgggt gcaaaaagtc
     6661 gactaaaacg aaaggctcag tcgaaagact gggcctttcg ttttacaatt gggctacctt
     6721 ttttttgttt tgtttgcaat ggttaatcta ttgttaaaat ttaaagtttc acttgaactt
     6781 tcaaataatg ttcttatatt tgcagtgtcg aaagaaacaa agtaggactg atcacacaac
     6841 atttaaaaaa taacattatg tctagattag ataaaagtaa agtgattaac agcgcattag
     6901 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
     6961 gtgtagagca gc
//
