LOCUS       pSI.        3632 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian cell expression vector with the SV40 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pSI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..413
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          483..615
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        660..678
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    678..730
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    complement(746..867)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1048..1503
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1835..1939
                     /label=AmpR promoter
     CDS             1940..2797
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2971..3559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
       61 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
      121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
      421 attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc
      481 aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga
      541 gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg
      601 cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtactt
      661 aatacgactc actataggct agcctcgaga attcacgcgt ggtacctcta gagtcgaccc
      721 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
      781 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      841 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
      901 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
      961 atcgataagg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1021 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     1081 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1141 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1201 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1261 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1321 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1381 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1441 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     1501 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     1561 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     1621 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     1681 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     1741 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1801 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1861 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1921 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1981 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2041 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2101 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2161 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     2221 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     2281 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     2341 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     2401 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     2461 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     2521 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     2581 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2641 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2701 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2761 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2821 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2881 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2941 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3001 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3061 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3121 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3181 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3241 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3301 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3361 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3421 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3481 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3541 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3601 ctggcctttt gctcacatgg ctcgacagat ct
//
