LOCUS       40924_40292        3359 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI-DSZcx-SV40, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 3359)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3359)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(7..395)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(416..449)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        508..837
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     protein_bind    913..934
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        949..979
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    987..1003
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1011..1027
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1045..1063
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1256..1303
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1413..1422
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1453..1471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1478..1494)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1783..2007
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2115..2903)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gaattctgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag
       61 ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt
      121 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
      181 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga
      241 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac
      301 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
      361 tactatcaac aggttgaact gctgatcaac agatcctcta cgcggccgcg gtaccataac
      421 ttcgtatagc atacattata cgaagttatc tggtttgatc ggcgcgccta gttattaata
      481 gtaatcaatt acggggtcct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      541 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      601 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      661 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      721 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
      781 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      841 aagcttggta ccgagctcaa aggagatata ccatgggaag agctggaaag cgggcagtga
      901 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      961 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     1021 ctatgaccat gattacgcca agctatttag gtgacactat agaatactca agctatgcat
     1081 ccaacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcact agtgattgat
     1141 cgtcgacgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     1201 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     1261 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     1321 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     1381 gtagggataa cagggtaatg ataatcccgc ggccatggcg gccgggagca tgcgacgtcg
     1441 ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     1501 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     1561 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     1621 tgaatggcga atggtgatca cccgggatct cgagccatgg tgctagcagc tgatgcatag
     1681 catgcggtac cgggagatgg gggaggctaa ctgaaacacg gaagggctct tcctgaccgc
     1741 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     1801 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1861 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1921 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     1981 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     2041 ggttaattaa gatcgatccc taggagtcca agcgagctcg atatccgtcg gcttgaacga
     2101 attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat
     2161 tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt
     2221 ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca
     2281 gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta
     2341 agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt
     2401 tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct
     2461 ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg
     2521 tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat
     2581 tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg
     2641 acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg
     2701 ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca
     2761 atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc
     2821 aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact
     2881 tcggcgatca ccgcttccct catgatgttt aactttgttt tagggcgact gccctgctgc
     2941 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     3001 ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc
     3061 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
     3121 atacgctact taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg
     3181 cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt
     3241 attctgcctc ccagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct
     3301 acgtgttccg cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagct
//
