LOCUS       40924_40287        3730 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI-DSZ2x-DM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 3730)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3730)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(7..395)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(416..449)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        525..828
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        830..1029
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1069..1178
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    1284..1305
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1320..1350
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1358..1374
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1382..1398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1416..1434
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1627..1674
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1784..1793
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1824..1842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1849..1865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2154..2378
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2486..3274)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gaattctgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag
       61 ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt
      121 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
      181 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga
      241 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac
      301 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
      361 tactatcaac aggttgaact gctgatcaac agatcctcta cgcggccgcg gtaccataac
      421 ttcgtatagc atacattata cgaagttatc tggtttggcg cgcctagtta ttaatagtaa
      481 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      541 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      601 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      661 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      721 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      781 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatgct gatgcggttt
      841 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      901 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      961 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1021 ataagcagac gtcgtttagt gaaccgtcag atcaccatcc agtatacgga cctttaattc
     1081 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     1141 taaaatacta tactgtaaat tacattttat ttacaatcac tcgacgaaga cttgatcacc
     1201 cgggatctcg aaagcttggt accgagctca aaggagatat accatgggaa gagctggaaa
     1261 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1321 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1381 acaggaaaca gctatgacca tgattacgcc aagctattta ggtgacacta tagaatactc
     1441 aagctatgca tccaacgcgt tgggagctct cccatatggt cgacctgcag gcggccgcac
     1501 tagtgattga tcgtcgacgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     1561 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     1621 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     1681 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     1741 aatacgcccg cgtagggata acagggtaat gataatcccg cggccatggc ggccgggagc
     1801 atgcgacgtc gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt
     1861 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     1921 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     1981 gttgcgcagc ctgaatggcg aatggtgatc acccgggatc tcgagccatg gtgctagcag
     2041 ctgatgcata gcatgcggta ccgggagatg ggggaggcta actgaaacac ggaagggctc
     2101 ttcctgaccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     2161 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2221 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2281 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2341 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     2401 ctggggctct aggttaatta agatcgatcc ctaggagtcc aagcgagctc gatatccgtc
     2461 ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt
     2521 agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
     2581 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
     2641 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
     2701 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
     2761 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
     2821 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
     2881 ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc
     2941 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
     3001 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt
     3061 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
     3121 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
     3181 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
     3241 gagtcgatac ttcggcgatc accgcttccc tcatgatgtt taactttgtt ttagggcgac
     3301 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     3361 gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag
     3421 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     3481 tgcgtgagcg catacgctac ttaccggttt attgactacc ggaagcagtg tgaccgtgtg
     3541 cttctcaaat gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga
     3601 tatgatcatt tattctgcct cccagctgac attcatccgg ggtcagcacc gtttctgcgg
     3661 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca
     3721 gcgtgaagct
//
