LOCUS       40924_40282        4188 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI-DSZ2cx-UBC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 4188)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4188)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(7..395)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(416..449)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        503..902
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     protein_bind    1742..1763
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1778..1808
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1816..1832
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1840..1856
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1874..1892
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2085..2132
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      2242..2251
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2282..2300)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2307..2323)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2612..2836
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2944..3732)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gaattctgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag
       61 ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt
      121 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
      181 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga
      241 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac
      301 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
      361 tactatcaac aggttgaact gctgatcaac agatcctcta cgcggccgcg gtaccataac
      421 ttcgtatagc atacattata cgaagttatc tggtttggcg cgcctagtta ttaatagtaa
      481 tcaattacgg ggtcggtgca gcggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
      541 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcaggagcgt tcctgatcct
      601 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
      661 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
      721 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
      781 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
      841 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
      901 gtgagttgcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
      961 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
     1021 gaactggggg ttggggggag cgcacaaaat ggcggctgtt cccgagtctt gaatggaaga
     1081 cgcttgtaag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     1141 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     1201 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcgggtttgt
     1261 cgtctggttg cgggggcggc agttatgcgg tgccgttggg cagtgcaccc gtacctttgg
     1321 gagcgcgcgc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     1381 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     1441 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     1501 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     1561 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     1621 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa agcttggtac
     1681 cgagctcaaa ggagatatac catgggaaga gctggaaagc gggcagtgag cgcaacgcaa
     1741 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     1801 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     1861 attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc caacgcgttg
     1921 ggagctctcc catatggtcg acctgcaggc ggccgcacta gtgattgatc gtcgacggat
     1981 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     2041 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     2101 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     2161 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tagggataac
     2221 agggtaatga taatcccgcg gccatggcgg ccgggagcat gcgacgtcgg gcccaattcg
     2281 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     2341 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2401 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2461 tggtgatcac ccgggatctc gagccatggt gctagcagct gatgcatagc atgcggtacc
     2521 gggagatggg ggaggctaac tgaaacacgg aagggctctt cctgaccgct cgagtctaga
     2581 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     2641 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2701 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2761 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     2821 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag gttaattaag
     2881 atcgatccct aggagtccaa gcgagctcga tatccgtcgg cttgaacgaa ttgttagaca
     2941 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     3001 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     3061 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     3121 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     3181 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     3241 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     3301 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     3361 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     3421 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     3481 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     3541 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     3601 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     3661 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     3721 cgcttccctc atgatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3781 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     3841 aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     3901 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     3961 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     4021 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     4081 cagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc
     4141 ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgaagct
//
