LOCUS       40924_40277        3431 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI-DSZ2cx-TRE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 3431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(7..395)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(416..449)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(513..783)
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     protein_bind    985..1006
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1021..1051
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1059..1075
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1083..1099
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1117..1135
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1328..1375
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1485..1494
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1525..1543)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1550..1566)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1855..2079
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2187..2975)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gaattctgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag
       61 ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt
      121 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
      181 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga
      241 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac
      301 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
      361 tactatcaac aggttgaact gctgatcaac agatcctcta cgcggccgcg gtaccataac
      421 ttcgtatagc atacattata cgaagttatc tggtttggcg cgcctagtta ttaatagtaa
      481 tcaattacgg ggtcgagctc gactttcact tttctctatc actgataggg agtggtaaac
      541 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
      601 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
      661 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
      721 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
      781 ggagtggtaa actcgaccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      841 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc
      901 ggccccgaat tgaagcttgg taccgagctc aaaggagata taccatggga agagctggaa
      961 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     1021 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     1081 cacaggaaac agctatgacc atgattacgc caagctattt aggtgacact atagaatact
     1141 caagctatgc atccaacgcg ttgggagctc tcccatatgg tcgacctgca ggcggccgca
     1201 ctagtgattg atcgtcgacg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     1261 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     1321 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     1381 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     1441 caatacgccc gcgtagggat aacagggtaa tgataatccc gcggccatgg cggccgggag
     1501 catgcgacgt cgggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     1561 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     1621 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     1681 agttgcgcag cctgaatggc gaatggtgat cacccgggat ctcgagccat ggtgctagca
     1741 gctgatgcat agcatgcggt accgggagat gggggaggct aactgaaaca cggaagggct
     1801 cttcctgacc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     1861 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     1921 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     1981 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2041 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2101 gctggggctc taggttaatt aagatcgatc cctaggagtc caagcgagct cgatatccgt
     2161 cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc gcctttcacg
     2221 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca
     2281 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
     2341 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
     2401 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
     2461 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
     2521 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
     2581 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
     2641 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
     2701 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
     2761 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
     2821 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
     2881 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
     2941 tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt tttagggcga
     3001 ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
     3061 cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa
     3121 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     3181 ttgcgtgagc gcatacgcta cttaccggtt tattgactac cggaagcagt gtgaccgtgt
     3241 gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg
     3301 atatgatcat ttattctgcc tcccagctga cattcatccg gggtcagcac cgtttctgcg
     3361 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc
     3421 agcgtgaagc t
//
