LOCUS       40924_40272        3524 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI-DSZ2cx-PGK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 3524)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3524)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(7..395)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(416..449)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        502..1001
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    1078..1099
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1114..1144
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1152..1168
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1176..1192
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1210..1228
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1421..1468
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1578..1587
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1618..1636)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1643..1659)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1948..2172
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2280..3068)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gaattctgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag
       61 ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt
      121 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
      181 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga
      241 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac
      301 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
      361 tactatcaac aggttgaact gctgatcaac agatcctcta cgcggccgcg gtaccataac
      421 ttcgtatagc atacattata cgaagttatc tggtttggcg cgcctagtta ttaatagtaa
      481 tcaattacgg ggtcttctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
      541 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
      601 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
      661 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
      721 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
      781 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
      841 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
      901 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
      961 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctaagct tggtaccgag
     1021 ctcaaaggag atataccatg ggaagagctg gaaagcgggc agtgagcgca acgcaattaa
     1081 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     1141 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     1201 cgccaagcta tttaggtgac actatagaat actcaagcta tgcatccaac gcgttgggag
     1261 ctctcccata tggtcgacct gcaggcggcc gcactagtga ttgatcgtcg acggatgatc
     1321 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     1381 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     1441 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     1501 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtagg gataacaggg
     1561 taatgataat cccgcggcca tggcggccgg gagcatgcga cgtcgggccc aattcgccct
     1621 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1681 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1741 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggt
     1801 gatcacccgg gatctcgagc catggtgcta gcagctgatg catagcatgc ggtaccggga
     1861 gatgggggag gctaactgaa acacggaagg gctcttcctg accgctcgag tctagagggc
     1921 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     1981 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2041 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2101 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     2161 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggtta attaagatcg
     2221 atccctagga gtccaagcga gctcgatatc cgtcggcttg aacgaattgt tagacattat
     2281 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     2341 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     2401 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     2461 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     2521 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     2581 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     2641 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     2701 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     2761 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     2821 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     2881 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     2941 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     3001 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     3061 tccctcatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     3121 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     3181 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     3241 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttaccg
     3301 gtttattgac taccggaagc agtgtgaccg tgtgcttctc aaatgcctga ggccagtttg
     3361 ctcaggctct ccccgtggag gtaataattg acgatatgat catttattct gcctcccagc
     3421 tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgtg ttccgcttcc
     3481 tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga agct
//
