LOCUS       40924_40267        3905 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI-DSZ2cx-DI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 3905)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3905)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(7..395)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(416..449)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        525..827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        828..1031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1213..1322
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    1459..1480
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1495..1525
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1533..1549
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1557..1573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1591..1609
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1802..1849
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1959..1968
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1999..2017)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2024..2040)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2329..2553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2661..3449)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gaattctgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag
       61 ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt
      121 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
      181 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga
      241 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac
      301 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
      361 tactatcaac aggttgaact gctgatcaac agatcctcta cgcggccgcg gtaccataac
      421 ttcgtatagc atacattata cgaagttatc tggtttggcg cgcctagtta ttaatagtaa
      481 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      541 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      601 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      661 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      721 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      781 tttcctactt ggcagtacat ctagtattag tcatcgctat taccatggtg atgcggtttt
      841 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      901 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      961 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1021 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     1081 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     1141 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagactctat aggcacaccc
     1201 ctttggctct tggaccttta attcaaccca acacaatata ttatagttaa ataagaatta
     1261 ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa
     1321 tcagacagac tgttcctttc ctgggtcttt tctgcaggca ccgtcgtcga cttaacagat
     1381 ctgtttaagc ttggtaccga gctcaaagga gatataccat gggaagagct ggaaagcggg
     1441 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     1501 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     1561 aaacagctat gaccatgatt acgccaagct atttaggtga cactatagaa tactcaagct
     1621 atgcatccaa cgcgttggga gctctcccat atggtcgacc tgcaggcggc cgcactagtg
     1681 attgatcgtc gacggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1741 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     1801 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     1861 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     1921 gcccgcgtag ggataacagg gtaatgataa tcccgcggcc atggcggccg ggagcatgcg
     1981 acgtcgggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     2041 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2101 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2161 gcagcctgaa tggcgaatgg tgatcacccg ggatctcgag ccatggtgct agcagctgat
     2221 gcatagcatg cggtaccggg agatggggga ggctaactga aacacggaag ggctcttcct
     2281 gaccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     2341 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     2401 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     2461 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     2521 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     2581 gctctaggtt aattaagatc gatccctagg agtccaagcg agctcgatat ccgtcggctt
     2641 gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg
     2701 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     2761 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     2821 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     2881 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     2941 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     3001 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     3061 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     3121 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     3181 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     3241 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     3301 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     3361 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     3421 gatacttcgg cgatcaccgc ttccctcatg atgtttaact ttgttttagg gcgactgccc
     3481 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
     3541 tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg
     3601 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     3661 gagcgcatac gctacttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct
     3721 caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga
     3781 tcatttattc tgcctcccag ctgacattca tccggggtca gcaccgtttc tgcggactgg
     3841 ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg
     3901 aagct
//
