LOCUS       40924_40232        3839 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI-DAZ-DI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 3839)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3839)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        71..373
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        374..577
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        759..868
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    1005..1026
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1041..1071
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1079..1095
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1103..1119
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1137..1155
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1348..1395
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1505..1514
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1545..1563)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1570..1586)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1875..2099
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2241..2345
                     /label=AmpR promoter
     CDS             2346..3203
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(3378..3759)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(3801..3834)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttggcgcgcc tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
       61 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      121 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      181 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      241 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      301 atgcccagta catgacctta tgggactttc ctacttggca gtacatctag tattagtcat
      361 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      421 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      481 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      541 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
      601 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
      661 ccgcggccgg gaacggtgca ttggaacgcg gattccccgt gccaagagtg acgtaagtac
      721 cgcctataga ctctataggc acaccccttt ggctcttgga cctttaattc aacccaacac
      781 aatatattat agttaaataa gaattattat caaatcattt gtatattaat taaaatacta
      841 tactgtaaat tacattttat ttacaatcag acagactgtt cctttcctgg gtcttttctg
      901 caggcaccgt cgtcgactta acagatctgt ttaagcttgg taccgagctc aaaggagata
      961 taccatggga agagctggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     1021 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     1081 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt
     1141 aggtgacact atagaatact caagctatgc atccaacgcg ttgggagctc tcccatatgg
     1201 tcgacctgca ggcggccgca ctagtgattg atcgtcgacg gatgatcctc cagcgcgggg
     1261 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     1321 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     1381 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     1441 caccgagacc ccattggggc caatacgccc gcgtagggat aacagggtaa tgataatccc
     1501 gcggccatgg cggccgggag catgcgacgt cgggcccaat tcgccctata gtgagtcgta
     1561 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1621 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1681 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggtgat cacccgggat
     1741 ctcgagccat ggtgctagca gctgatgcat agcatgcggt accgggagat gggggaggct
     1801 aactgaaaca cggaagggct cttcctgacc gctcgagtct agagggcccg tttaaacccg
     1861 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     1921 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     1981 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2041 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     2101 ttctgaggcg gaaagaacca gctggggctc taggttaatt aagatcgatc cctaggcttt
     2161 acgtaactat aacggtccta aggtagcgaa taccgtcgac ctctagccct gcaggaggtg
     2221 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2281 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2341 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2401 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2461 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2521 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2581 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2641 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2701 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2761 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2821 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2881 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2941 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3001 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3061 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3121 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3181 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3301 tcatgaccaa aatcccttaa cgtgagttta aagaacatgt gagcacctga gtgcttgcgg
     3361 cagcgtgaag ctaattctgt cagccgttaa gtgttcctgt gtcactcaaa attgctttga
     3421 gaggctctaa gggcttctca gtgcgttaca tccctggctt gttgtccaca accgttaaac
     3481 cttaaaagct ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga
     3541 ggctatttaa gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt
     3601 gaaacatgag agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta
     3661 gttcgttaaa catgagagct tagtacgtta aacatgagag cttagtacgt gaaacatgag
     3721 agcttagtac gtactatcaa caggttgaac tgctgatcga tcttttctac ggggcggccg
     3781 cgtcagtcag tcatcatacc ataacttcgt atagcataca ttatacgaag ttatctggt
//
