LOCUS       40924_40202        4148 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI-AGZ10, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 4148)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4148)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(33..198)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      388..976
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(1111..1334)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1404..1934)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(2123..2151)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2222..2255)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2347..2650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2651..2854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2899..2917
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3019..3040
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3055..3085
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3093..3109
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3117..3133
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3151..3169
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3362..3409
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      3519..3528
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3559..3577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3584..3600)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3889..4113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttaattaacc tagggtatac ccatctaatt ggaaccagat aagtgaaatc tagttccaaa
       61 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
      121 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
      181 ctattttgtc cgcccacaac cggttgactt gggtcaactg tcagaccaag tttactcata
      241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1021 gccttttgct cacatgttct ttcctgcgtt atcccctgat tgacttgggt cactcttcct
     1081 gtggatgcgc agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     1141 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     1201 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     1261 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     1321 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     1381 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     1441 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     1501 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     1561 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     1621 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     1681 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     1741 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     1801 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     1861 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     1921 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     1981 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
     2041 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     2101 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     2161 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcgc
     2221 cataacttcg tatagcatac attatacgaa gttatctgta actataacgg tcctaagtgg
     2281 cgcgcctagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2341 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2401 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2461 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2521 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2581 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2641 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2701 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2761 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2821 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
     2881 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt
     2941 aaacttaagc ttggtaccga gctcaaagga gatataccat gggaagagct ggaaagcggg
     3001 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     3061 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     3121 aaacagctat gaccatgatt acgccaagct atttaggtga cactatagaa tactcaagct
     3181 atgcatccaa cgcgttggga gctctcccat atggtcgacc tgcaggcggc cgcactagtg
     3241 attgatcgtc gacggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     3301 cataggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     3361 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     3421 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     3481 gcccgcgtag ggataacagg gtaatgataa tcccgcggcc atggcggccg ggagcatgcg
     3541 acgtcgggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     3601 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     3661 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     3721 gcagcctgaa tggcgaatgg tgatcacccg ggatctcgag ccatggtgct agcagctgat
     3781 gcatagcatg cggtaccggg anatggggga ggctaactga aacacggaag ggctcttcct
     3841 gaccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     3901 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3961 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4021 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4081 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4141 gctctagg
//
