LOCUS       40924_40182        4410 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSI-AGL10-DI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Mansouri M, Bellon-Echeverria I, Rizk A, Ehsaei Z, Cianciolo 
            Cosentino C, Silva CS, Xie Y, Boyce FM, Davis MW, Neuhauss SC, 
            Taylor V, Ballmer-Hofer K, Berger I, Berger P.
  TITLE     Highly efficient baculovirus-mediated multigene delivery in primary 
            cells
  JOURNAL   Nat Commun 7, 11529 (2016)
  PUBMED    27143231
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Mansouri M, Berger I, Berger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Laboratory of Biomolecular Research, Paul 
            Scherrer Institute, OFLG-110, Villigen psi 5232, Switzerland
REFERENCE   3  (bases 1 to 4410)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            7, 11529 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2016) Laboratory of Biomolecular Research, Paul Scherrer 
            Institute, OFLG-110, Villigen psi 5232, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Ape v. v. v2.0.49
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(913..1136)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1206..1736)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1925..1953)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2149..2451
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2452..2655
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2837..2946
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    3083..3104
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3119..3149
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3157..3173
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3181..3197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3215..3233
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3426..3473
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      3583..3592
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3623..3641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3648..3664)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3953..4177
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     mobile_element  complement(4245..4410)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcactcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagt ggcgcgccta gttattaata
     2101 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2161 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2221 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2281 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2341 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2401 ggactttcct acttggcagt acatctagta ttagtcatcg ctattaccat ggtgatgcgg
     2461 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     2521 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     2581 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     2641 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
     2701 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt
     2761 ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagact ctataggcac
     2821 acccctttgg ctcttggacc tttaattcaa cccaacacaa tatattatag ttaaataaga
     2881 attattatca aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt
     2941 acaatcagac agactgttcc tttcctgggt cttttctgca ggcaccgtcg tcgacttaac
     3001 agatctgttt aagcttggta ccgagctcaa aggagatata ccatgggaag agctggaaag
     3061 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     3121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     3181 caggaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca
     3241 agctatgcat ccaacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcact
     3301 agtgattgat cgtcgacgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3361 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     3421 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     3481 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     3541 atacgcccgc gtagggataa cagggtaatg ataatcccgc ggccatggcg gccgggagca
     3601 tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     3661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3781 ttgcgcagcc tgaatggcga atggtgatca cccgggatct cgagccatgg tgctagcagc
     3841 tgatgcatag catgcggtac cgggagatgg gggaggctaa ctgaaacacg gaagggctct
     3901 tcctgaccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     3961 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4021 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4081 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4141 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4201 tggggctcta ggttaattaa cctagggtat acccatctaa ttggaaccag ataagtgaaa
     4261 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
     4321 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
     4381 cccagttccc aactattttg tccgcccaca
//
