LOCUS       V003223                 7871 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003223
VERSION     V003223
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Wang Y, Su H, Li G, Yang M, Yi J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2017) School of Environmental and Biological
            Engineering, Nanjing University of Science and Technology,
            XiaoLinwei 200#, Nan Jing, JiangSu 210094, China
REFERENCE   2  (bases 1 to 7871)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7871)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2017) School of Environmental and Biological Engineering,
            Nanjing University of Science and Technology, XiaoLinwei 200#, Nan
            Jing, JiangSu 210094, China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..504
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        769..989
                     /label="URA3 promoter"
     CDS             990..1790
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(1924..2379)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2524..2540
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2550..2568
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2587..2834)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(3379..4083)
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     protein_bind    complement(4093..4109)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4147..4163)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(4178..4619)
                     /label="GAL1 promoter"
                     /note="inducible promoter, regulated by Gal4"
     rep_origin      4651..5181
                     /label="oriV"
                     /note="incP origin of replication"
     regulatory      5403..5521
                     /label="neo promoter"
                     /note="neo promoter"
                     /regulatory_class="promoter"
     CDS             5573..6718
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     regulatory      6772..7377
                     /regulatory_class="terminator"
     regulatory      7381..7434
                     /label="hbs promoter"
                     /note="hbs promoter"
                     /regulatory_class="promoter"
     CDS             7435..7806
                     /label="BleoR"
                     /note="antibiotic-binding protein"
ORIGIN
        1 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
       61 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
      121 actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat
      181 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
      241 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
      301 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
      361 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
      421 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
      481 cgtgtaagtt acaggcaagc gatccgtcct aagaaaccat tattatcatg acattaacct
      541 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
      601 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      661 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
      721 atgcggcatc agagcagatt gtactgagag tgcaccatac cacagctttt caattcaatt
      781 catcattttt tttttattct tttttttgat ttcggtttct ttgaaatttt tttgattcgg
      841 taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
      901 gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
      961 caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
     1021 ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
     1081 acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
     1141 taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
     1201 agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag
     1261 acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
     1321 gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
     1381 gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
     1441 cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
     1501 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
     1561 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
     1621 acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
     1681 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
     1741 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
     1801 tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
     1861 agttattacc ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1921 aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     1981 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     2041 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     2101 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     2161 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     2221 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     2281 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     2341 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg ccattcaggc
     2401 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2461 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2521 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
     2581 ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg ttttcagtat
     2641 aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa atataaataa
     2701 cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt caggttgtct
     2761 aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta agcgtgacat
     2821 aactaattac atgactcgag gtcgacaatt ccaaccttac ccaagagttc gccaaactca
     2881 gacatcactt tangcaaaac cgcgccgtgc ttcttcctcg gtggcattca tcacgaaatg
     2941 ttcagcacta cgcatacttt tgacaggaaa cgcaacggat attgagtcaa tatcaggcat
     3001 tctatcgctc agctttacag tgacaatgac ggctggcgac tgaatattag tgcttacaga
     3061 cagcactaca tattttccgt cgatgttgaa atcctttctc atatgtcacc ataaatatca
     3121 aataattata gcaatcattt acgcgttaat ggctaatcgc catcttccag caggcgcacc
     3181 attgcccctg tttcactatc cagggtacgg atatagttca tgacaatatt tacattggtc
     3241 cagccaccag cttgcatgat ctccggtatt gaaactccag cgcgggccat atctcgcgcg
     3301 gctccgacac gggcactgtg tccagaccag gccaggtatc tctgaccaga gtcatcctta
     3361 gcgccgtaaa tcaatttatt tcaggaaagt ttcggaggag atagtgttcg gcagtttgta
     3421 catcatctgc gggatcaggt acggtttgat caggttgtag aagatcaggt aagacataga
     3481 atcgatgtag atgatcggtt tgtttttgtt gatttttacg taacagttca gttggaattt
     3541 gttacgcaga cccttaacca ggtattctac ttcttcgaaa gtgaaagact gggtgttcag
     3601 tacgatcgat ttgttggtag agtttttgtt gtaatcccat ttaccaccat catccatgaa
     3661 ccagtatgcc agagacatcg gggtcaggta gttttcaacc aggttgttcg ggatggtttt
     3721 tttgttgtta acgatgaaca ggttagccag tttgttgaaa gcttggtgtt tgaaagtctg
     3781 ggcgccccag gtgattacca ggttacccag gtggttaaca cgttcttttt tgtgcggcgg
     3841 ggacagtacc cactgatcgt acagcagaca tacgtggtcc atgtatgctt tgtttttcca
     3901 ctcgaactgc atacagtagg ttttaccttc atcacgagaa cggatgtaag catcacccag
     3961 gatcagaccg atacctgctt cgaactgttc gatgttcagt tcgatcagct gggatttgta
     4021 ttctttcagc agtttagagt tcggacccag gttcattacc tggttttttt tgatgttttt
     4081 catatgcatg aattgttatc cgctcacaat tccataagct tgatatcgaa ttcctgcagc
     4141 ccgggggatc cactagttct agaatccggg gttttttctc cttgacgttt aagtatagag
     4201 gcatattgac aattttttgt tgatactttt attacgtttg aataagaagt aatacaaacc
     4261 gaaaacgttg aaagtattag ttaaagtggt tatgcagttt ctgcatttat atatctgtta
     4321 atagatcaaa aatcatcgct tcgctaatta attaccccag aaataaggct aaaaaactaa
     4381 tcgcattatc atcctatggt tgttaatttg attcgttcat ttgaaggttt gtggggccag
     4441 gttactgcca atttttcctc ttcataacca taaaagctag tattgtagaa tctttattgt
     4501 tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt gaagacgagg
     4561 acgcacggag gagagtcttc cttcggaggg ctgtcacccg ctcggcggct tctaatccgt
     4621 actagagctc actacagcag agccatgtag ggccgccggc gttgtggata ccacgcggaa
     4681 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
     4741 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
     4801 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
     4861 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
     4921 ggggcgcgat ccttgacact tgaggggcag agtgatgaca gatgaggggc gcacctattg
     4981 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
     5041 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
     5101 tttaaccagg gctgcgccct ggcgcgtgac cgcgcacgcc gaaggggggt gccccccctt
     5161 ctcgaaccct cccggcccgc taacgcggca ccccatcccc ccaggggctg cgcccctcgg
     5221 ccgcgaacga cctcacccca aaaatggcag ccacgtagaa agccagtccg cagaaacggt
     5281 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     5341 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     5401 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     5461 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     5521 cgacggatcg atccggggaa ttaattccgg ggcaatcccg caaggagggt gaatgaatcg
     5581 gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt ccgccgagga
     5641 tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt
     5701 cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc aactggctcc
     5761 ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtctcg aacaggaggc
     5821 ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga ccaagaagcg
     5881 aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct
     5941 gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg
     6001 gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt tcaccacgcg
     6061 caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg tcaacaagga
     6121 cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac tggtgtggca
     6181 gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct tcacgttcta
     6241 cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga aggccgagga
     6301 atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg ttgggcacct
     6361 ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga aaacgtcccg
     6421 ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc actacacgaa
     6481 attcacatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt tcgactattt
     6541 cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca tgtgcggatc
     6601 ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg
     6661 aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt gcaaacgcta
     6721 gggccttgtg gggtcagttc cggctggggg ttcagcagcc accggcatgc aagcttgcta
     6781 gagggtcagc tttatgcttg taaaccgttt tgtgaaaaaa tttttaaaat aaaaaagggg
     6841 acctctaggg tccccaatta attagtaata taatctatta aaggtcattc aaaaggtcat
     6901 ccaccggatc aattcccctg ctcgcgcagg ctgggtgcca agctctcggg taacatcaag
     6961 gcccgatcct tggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc
     7021 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     7081 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     7141 gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc
     7201 aacatccgca ttaaaatcta gcgagggctt tactaagctg atccggtgga tgaccttttg
     7261 aatgaccttt aatagattat attactaatt aattggggac cctagaggtc ccctttttta
     7321 ttttaaaaat tttttcacaa aacggtttac aagcataaag ctgactctag ctagaggatc
     7381 cttgatatgg ctttttatat gtgttactct acatacagaa aggaggaact aaatatggcc
     7441 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     7501 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     7561 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     7621 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     7681 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     7741 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     7801 caggactgag gatcccctcg acttattatt tcaggaaagt ttcggaggag atagtgttcg
     7861 gcagtttgta c
//
