LOCUS       V003224                 4516 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003224
VERSION     V003224
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira
            T, Bull EH, Butt AT, Thomas MS.
  TITLE     An efficient system for the generation of marked genetic mutants in
            members of the genus Burkholderia
  JOURNAL   Plasmid (2016) In press
   PUBMED   27825973
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Shastri S, Spiewak HL, Pereira T, Sofoluwe AO, Eidsvaag VA, Bull EH,
            Asghar AH, Thomas MS.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4516)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4516)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid
            (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUN-2016) Department of Infection, Immunity "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             144..782
                     /gene="cmlA"
                     /label="Chloramphenicol acetyltransferase 2"
                     /note="Chloramphenicol acetyltransferase 2 from Escherichia
                     coli. Accession#: P22615"
     rep_origin      complement(930..1318)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1939..2307)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(2340..2449)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(3530..4387)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4388..4492)
                     /label="AmpR promoter"
ORIGIN
        1 tgcagatcct tgacaattaa gcccgtatat ggtattatta ctgaatacca aacagcttac
       61 ggaggacgga atgttaccca ttgagacaac cagactgcct tctgattatt aatatttttc
      121 actattaatc agaaggaata accatgaatt ttacccggat tgacctgaat acctggaatc
      181 gcagggaaca ctttgccctt tatcgtcagc agattaaatg cggattcagc ctgaccacca
      241 aactcgatat taccgctttg cgtaccgcac tggcggagac aggttataag ttttatccgc
      301 tgatgattta cctgatctcc cgggctgtta atcagtttcc ggagttccgg atggcactga
      361 aagacaatga acttatttac tgggaccagt cagacccggt ctttactgtc tttcataaag
      421 aaaccgaaac attctctgca ctgtcctgcc gttattttcc ggatctcagt gagtttatgg
      481 caggttataa tgcggtaacg gcagaatatc agcatgatac cagattgttt ccgcagggaa
      541 atttaccgga gaatcacctg aatatatcat cattaccgtg ggtgagtttt gacggattta
      601 acctgaacat caccggaaat gatgattatt ttgccccggt ttttacgatg gcaaagtttc
      661 agcaggaagg tgaccgcgta ttattacctg tttctgtaca ggttcatcat gcagtctgtg
      721 atggctttca tgcagcacgg tttattaata cacttcagct gatgtgtgat aacatactga
      781 aataaattaa ttaattctgt atttaagcca ccgtatccgg gagctcttag gcctttctcg
      841 agttggtacc tttctagatt agatctttgt cgacaagctt cggccgccta ggccgcggcc
      901 gcaaactagt aagggcccaa gaattcccat gtcagccgtt aagtgttcct gtgtcactca
      961 aaattgcttt gagaggctct aagggcttct cagtgcgtta catccctggc ttgttgtcca
     1021 caaccgttaa accttaaaag ctttaaaagc cttatatatt cttttttttc ttataaaact
     1081 taaaacctta gaggctattt aagttgctga tttatattaa ttttattgtt caaacatgag
     1141 agcttagtac gtgaaacatg agagcttagt acgttagcca tgagagctta gtacgttagc
     1201 catgagggtt tagttcgtta aacatgagag cttagtacgt taaacatgag agcttagtac
     1261 gtgaaacatg agagcttagt acgtactatc aacaggttga actgctgatc ttcagatcct
     1321 ctacgccgga cgcatcgtgg ccggatccag ccgaccaggc tttccacgcc cgcgtgccgc
     1381 tccatgtcgt tcgcgcggtt ctcggaaacg cgctgccgcg tttcgtgatt gtcacgctca
     1441 agcccgtagt cccgttcgag cgtcgcgcag aggtcagcga gggcgcggta ggcccgatac
     1501 ggctcatgga tggtgtttcg ggtcgggtga atcttgttga tggcgatatg gatgtgcagg
     1561 ttgtcggtgt cgtgatgcac ggcactgacg cgctgatgct cggcgaagcc aagcccagcg
     1621 cagatgcggt cctcaatcgc gcgcaacgtc tccgcgtcgg gcttctctcc cgcgcggaag
     1681 ctaaccagca ggtgataggt cttgtcggcc tcggaacggg tgttgccgtg ctgggtcgcc
     1741 atcacctcgg ccatgacagc gggcagggtg tttgcctcgc agttcgtgac gcgcacgtga
     1801 cccaggcgct cggtcttgcc ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg
     1861 ctcttcttga tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt
     1921 tagcggctaa aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc
     1981 aagctcgtcc tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa
     2041 ccgcgccgtg cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag
     2101 gtcgccattg atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt
     2161 gtagccctgg ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt
     2221 ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt
     2281 cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc
     2341 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
     2401 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
     2461 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa
     2521 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga
     2581 aaagcgctgc ttccctgctg ttttgtggaa tatctaccga ctggaaacag gcaaatgcag
     2641 gaaattactg aactgagggg acaggcgaga gacgatgcca aagagctaca ccgacgagct
     2701 ggccgagtgg gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc
     2761 gttcctggcg gtgagggcgg atgtcgaggc ggcgttagcg tccggctatg cgctcgtcac
     2821 catttgggag cacatgcggg aaacggggaa ggtcaagttc tcctacgaga cgttccgctc
     2881 gcacgccagg cggcacatca aggccaagcc cgccgatgtg cccgcaccgc aggccaaggc
     2941 tgcggaaccc gcgccggcac ccaagacgcc ggagccacgg cggccgaagc aggggggcaa
     3001 ggctgaaaag ccggcccccg ctgcggcccc gaccggcttc accttcaacc caacaccgga
     3061 caaaaaggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     3121 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     3181 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     3241 gcgccatctc cttgctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     3301 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     3361 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     3421 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     3481 accataaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3541 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3601 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3661 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3721 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3781 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3841 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3901 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3961 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4021 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4081 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4141 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4201 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4261 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4321 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4381 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4441 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4501 cccgaaaagt gccacc
//
