LOCUS       V003225                 4570 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003225
VERSION     V003225
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira
            T, Bull EH, Butt AT, Thomas MS.
  TITLE     An efficient system for the generation of marked genetic mutants in
            members of the genus Burkholderia
  JOURNAL   Plasmid (2016) In press
   PUBMED   27825973
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Shastri S, Spiewak HL, Pereira T, Sofoluwe AO, Eidsvaag VA, Bull EH,
            Asghar AH, Thomas MS.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid
            (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUN-2016) Department of Infection, Immunity "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             144..782
                     /gene="cmlA"
                     /label="Chloramphenicol acetyltransferase 2"
                     /note="Chloramphenicol acetyltransferase 2 from Escherichia
                     coli. Accession#: P22615"
     misc_feature    903..921
                     /label="O end"
                     /note="O end"
     protein_bind    complement(933..949)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      complement(984..1372)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1993..2361)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(2394..2503)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(3584..4441)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4442..4546)
                     /label="AmpR promoter"
ORIGIN
        1 tgcagatcct tgacaattaa gcccgtatat ggtattatta ctgaatacca aacagcttac
       61 ggaggacgga atgttaccca ttgagacaac cagactgcct tctgattatt aatatttttc
      121 actattaatc agaaggaata accatgaatt ttacccggat tgacctgaat acctggaatc
      181 gcagggaaca ctttgccctt tatcgtcagc agattaaatg cggattcagc ctgaccacca
      241 aactcgatat taccgctttg cgtaccgcac tggcggagac aggttataag ttttatccgc
      301 tgatgattta cctgatctcc cgggctgtta atcagtttcc ggagttccgg atggcactga
      361 aagacaatga acttatttac tgggaccagt cagacccggt ctttactgtc tttcataaag
      421 aaaccgaaac attctctgca ctgtcctgcc gttattttcc ggatctcagt gagtttatgg
      481 caggttataa tgcggtaacg gcagaatatc agcatgatac cagattgttt ccgcagggaa
      541 atttaccgga gaatcacctg aatatatcat cattaccgtg ggtgagtttt gacggattta
      601 acctgaacat caccggaaat gatgattatt ttgccccggt ttttacgatg gcaaagtttc
      661 agcaggaagg tgaccgcgta ttattacctg tttctgtaca ggttcatcat gcagtctgtg
      721 atggctttca tgcagcacgg tttattaata cacttcagct gatgtgtgat aacatactga
      781 aataaattaa ttaattctgt atttaagcca ccgtatccgg gagctcttag gcctttctcg
      841 agttggtacc tttctagatt agatctttgt cgacaagctt cggccgccta ggccgcggcc
      901 gcacttgtgt ataagagtca gaattccaca aattgttatc cgctcacaat tccacatgtg
      961 gaattccaca tgtggaattc ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg
     1021 ctttgagagg ctctaagggc ttctcagtgc gttacatccc tggcttgttg tccacaaccg
     1081 ttaaacctta aaagctttaa aagccttata tattcttttt tttcttataa aacttaaaac
     1141 cttagaggct atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta
     1201 gtacgtgaaa catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag
     1261 ggtttagttc gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtgaaa
     1321 catgagagct tagtacgtac tatcaacagg ttgaactgct gatcttcaga tcctctacgc
     1381 cggacgcatc gtggccggat ccagccgacc aggctttcca cgcccgcgtg ccgctccatg
     1441 tcgttcgcgc ggttctcgga aacgcgctgc cgcgtttcgt gattgtcacg ctcaagcccg
     1501 tagtcccgtt cgagcgtcgc gcagaggtca gcgagggcgc ggtaggcccg atacggctca
     1561 tggatggtgt ttcgggtcgg gtgaatcttg ttgatggcga tatggatgtg caggttgtcg
     1621 gtgtcgtgat gcacggcact gacgcgctga tgctcggcga agccaagccc agcgcagatg
     1681 cggtcctcaa tcgcgcgcaa cgtctccgcg tcgggcttct ctcccgcgcg gaagctaacc
     1741 agcaggtgat aggtcttgtc ggcctcggaa cgggtgttgc cgtgctgggt cgccatcacc
     1801 tcggccatga cagcgggcag ggtgtttgcc tcgcagttcg tgacgcgcac gtgacccagg
     1861 cgctcggtct tgccttgctc gtcggtgatg tacttcacca gctccgcgaa gtcgctcttc
     1921 ttgatggagc gcatggggac gtgcttggca atcacgcgca ccccccggcc gttttagcgg
     1981 ctaaaaaagt catggctctg ccctcgggcg gaccacgccc atcatgacct tgccaagctc
     2041 gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc
     2101 cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg cccaggcggc ccaggtcgcc
     2161 attgatgcgg gccagctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc
     2221 ctggccgacg gccagcaggt aggccgacag gctcatgccg gccgccgccg ccttttcctc
     2281 aatcgctctt cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt
     2341 tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca
     2401 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     2461 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     2521 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     2581 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagcg
     2641 ctgcttccct gctgttttgt ggaatatcta ccgactggaa acaggcaaat gcaggaaatt
     2701 actgaactga ggggacaggc gagagacgat gccaaagagc tacaccgacg agctggccga
     2761 gtgggttgaa tcccgcgcgg ccaagaagcg ccggcgtgat gaggctgcgg ttgcgttcct
     2821 ggcggtgagg gcggatgtcg aggcggcgtt agcgtccggc tatgcgctcg tcaccatttg
     2881 ggagcacatg cgggaaacgg ggaaggtcaa gttctcctac gagacgttcc gctcgcacgc
     2941 caggcggcac atcaaggcca agcccgccga tgtgcccgca ccgcaggcca aggctgcgga
     3001 acccgcgccg gcacccaaga cgccggagcc acggcggccg aagcaggggg gcaaggctga
     3061 aaagccggcc cccgctgcgg ccccgaccgg cttcaccttc aacccaacac cggacaaaaa
     3121 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     3181 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     3241 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     3301 tctccttgct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3361 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     3421 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     3481 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     3541 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3661 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3721 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3781 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3841 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     3901 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3961 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4021 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4081 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4141 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     4201 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4261 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4321 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4381 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4441 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4501 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4561 aagtgccacc
//
