LOCUS       V003241                12520 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003241
VERSION     V003241
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12520)
  AUTHORS   Hon S, Holwerda EK, Worthen RS, Maloney MI, Tian L, Cui J, Lin PP,
            Lynd LR, Olson DG.
  TITLE     Expressing the Thermoanaerobacterium saccharolyticum pforA in
            engineered Clostridium thermocellum improves ethanol production
  JOURNAL   Biotechnol Biofuels 11, 242 (2018)
   PUBMED   30202437
REFERENCE   2  (bases 1 to 12520)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Worthen RS, Maloney MI, Tian L, Cui J,
            Lin PP, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 12520)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12520)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 11, 242 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-APR-2018) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..573
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(840..1418)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label="tdk"
                     /protein_id="AYA22182.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(840..1418)
                     /gene="tdk"
                     /label="tdk"
     regulatory      complement(1419..2039)
                     /label="C. thermocellum cbp promoter"
                     /note="C. thermocellum cbp promoter"
                     /regulatory_class="promoter"
     CDS             complement(2118..3119)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(3120..3574)
                     /direction=LEFT
                     /label="C. thermocellum origin of replication"
                     /note="C. thermocellum origin of replication"
     CDS             complement(3680..4537)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4538..4642)
                     /label="AmpR promoter"
     misc_feature    4732..5266
                     /label="upstream homology recombination flank"
                     /note="upstream homology recombination flank"
     regulatory      5267..5465
                     /label="C. bescii Athe_2105 promoter"
                     /note="C. bescii Athe_2105 promoter"
                     /regulatory_class="promoter"
     CDS             5466..8993
                     /codon_start=1
                     /gene="pforA"
                     /product="pyruvate ferredoxin oxidoreductase"
                     /label="pforA"
                     /note="derived from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AYA22185.1"
                     /translation="MSKVMKTMDGNTAAAHVAYAFTEVAAIYPITPSSPMAEHVDEWSA
                     HGRKNLFGQEVKVIEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMYKIAGEL
                     LPGVFHVSARALASHALSIFGDHQDVMACRQTGFALLASGSVQEVMDLGSVAHLAAIKG
                     RVPFLHFFDGFRTSHEYQKIEVMDYEDLRKLLDMDAVREFKKRALNPEHPVTRGTAQNP
                     DIYFQEREASNRYYNAVPEIVEEYMKEISKITGREYKLFNYYGAPDAERIVIAMGSVTE
                     TIEETIDYLLKKGEKVGVVKVHLYRPFSFKHFMDAIPKTVKKIAVLDRTKEAGAFGEPL
                     YEDVRAAFYDSEMKPIIVGGRYGLGSKDTTPAQIVAVFDNLKSDTPKNNFTIGIVDDVT
                     YTSLPVGEEIETTAEGTISCKFWGFGSDGTVGANKSAIQIIGDNTDMYAQAYFSYDSKK
                     SGGVTISHLRFGKKPIRSTYLINNADFVACHKQAYVYNYDVLAGLKKGGTFLLNCTWKP
                     EELDEKLPASMKRYIAKNNINFYIINAVDIAKELGLGARINMIMQSAFFKLANIIPIDE
                     AVKHLKDAIVKSYGHKGEKIVNMNYAAVDRGIDALVKVDVPASWANAEDEAKVERNVPD
                     FIKNIADVMNRQEGDKLPVSAFVGMEDGTFPMGTAAYEKRGIAVDVPEWQIDNCIQCNQ
                     CAYVCPHAAIRPFLLNEEEVKNAPEGFTSKKAIGKGLEGLNFRIQVSVLDCTGCGVCAN
                     TCPSKEKSLIMKPLETQLDQAKNWEYAMSLSYKENPLGTDTVKGSQFEKPLLEFSGACA
                     GCGETPYARLVTQLFGDRMLIANATGCSSIWGGSAPSTPYTVNKDGHGPAWANSLFEDN
                     AEFGFGMALAVKQQREKLADIVKEALELDLTQDLKNALKLWLDNFNSSEITKKTANIIV
                     SLIQDYKTDDSKVKELLNEILDRKEYLVKKSQWIFGGDGWAYDIGFGGLDHVLASGEDV
                     NVLVFDTEVYSNTGGQSSKATPVGAIAQFAAAGKGIGKKDLGRIAMSYGYVYVAQIAMG
                     ANQAQTIKALKEAESYPGPSLIIAYAPCINHGIKLGMGCSQIEEKKAVEAGYWHLYRYN
                     PMLKAEGKNPFILDSKAPTASYKEFIMGEVRYSSLAKTFPERAEALFEKAEELAKEKYE
                     TYKKLAEQN"
     gene            5466..8993
                     /gene="pforA"
                     /label="pforA"
                     /note="Tsac_0046"
     CDS             9011..9181
                     /codon_start=1
                     /gene="Tsac_2084"
                     /product="ferredoxin"
                     /label="Tsac_2084"
                     /note="derived from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AYA22186.1"
                     /translation="MAHIITDECISCGACAAECPVDAIHEGTGKYEVDADTCIDCGACE
                     PVCPTGAIKAE"
     gene            9011..9181
                     /gene="Tsac_2084"
                     /label="Tsac_2084"
     misc_feature    9182..9741
                     /label="first downstream homology recombination flank"
                     /note="first downstream homology recombination flank"
     regulatory      9745..10319
                     /label="C. thermocellum gapDH promoter"
                     /note="C. thermocellum gapDH promoter"
                     /regulatory_class="promoter"
     CDS             10320..10967
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             10988..11533
                     /codon_start=1
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /protein_id="AYA22188.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            10988..11533
                     /gene="hpt"
                     /label="hpt"
     misc_feature    11718..12277
                     /label="second downstream homology recombination flank"
                     /note="second downstream homology recombination flank"
     terminator      12310..12498
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ttttatctga ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg
       61 ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa
      121 ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt
      181 ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg
      241 ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
      361 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
      421 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac
      481 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
      541 tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt
      601 ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt
      661 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata
      721 tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag
      781 cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacaaa aaaatccgct
      841 taagtccgcg ggacttcatg gcactttcta caccttgctt cataagactc ctttgctcca
      901 accattataa tgggatcgtc gtaattggca ggcttcccat ttataagcct ttgcgtgcga
      961 gtagcaggat tgccacactt catgcatata gcagtaagct tatcgacaaa ttcagctatg
     1021 gccatcaatt ctggagtcgg accaaatggt tcacctctaa agtccatgtc aagccctgca
     1081 catatgactc ttttcccgct atcggcaatc tcttttacga tgtcgactat ttcagaatca
     1141 aaaaattgaa cttcatctat agcaaataca tccgtatctt cttcagcata agccaatatg
     1201 tcagaagcct ttactatggc aatggcgtgc atgttgtcgc cgttatgaga tacgactttg
     1261 tctatggaat acctatcgtc tatagccggt ttgaaaacct gaactttttg cctggcaatc
     1321 ttagctctct ttatccttct tataagttcc tcactttttc cactgaacat gggaccagtt
     1381 acaacttcta tgtacccgtg gtctttaggc ccatacatct ttcgtcctcc ttaaaatttt
     1441 cgtttatttg ttttttattt agtgtgccta aaagcaccat cttatacatt cgtaccttat
     1501 atctcttatc ttgtatctct tacctcatat ctcttattat taactaccga agtatatttt
     1561 cataacggaa tcttatgact tatttttctg actgccgttt taccatacgg ttaatttata
     1621 cggaaacagc taatctaacc ttgctgaagg catgtttacc aaaatattat atctacttta
     1681 ccatattaat tcaagtggtc attttgatat tttacgccaa aatttttagc gttcctttac
     1741 ttgataattt atccaaaaac cttcattcgt caaaacaatt atgatataat taatttatga
     1801 tatgattaat taatatttac ggataactaa tcatttcaat gcaatagatc aacatgcaaa
     1861 tcaatatcca ccttactccg aacattttgc accaacgaat gcccagcact atatcccggc
     1921 aagcaatata acgtctataa ttttttatga taacgataaa attaaatttt atgaaagggg
     1981 taaaagcatg agaaaaatag ctgtattgtt aattactttg acgttcttag tcacgactcg
     2041 aatttaggag gcttacttgt ctgctttctt cattagaatc aatccttttt taaaagtcaa
     2101 tcccgtttgt tgaactactc tttaataaaa taatttttcc gttcccaatt ccacattgca
     2161 ataatagaaa atccatcttc atcggctttt tcgtcatcat ctgtatgaat caaatcgcct
     2221 tcttctgtgt catcaaggtt taatttttta tgtatttctt ttaacaaacc accataggag
     2281 attaaccttt tacggtgtaa accttcctcc aaatcagaca aacgtttcaa attcttttct
     2341 tcatcatcgg tcataaaatc cgtatccttt acaggatatt ttgcagtttc gtcaattgcc
     2401 gattgtatat ccgatttata tttatttttc ggtcgaatca tttgaacttt tacatttgga
     2461 tcatagtcta atttcattgc ctttttccaa aattgaatcc attgtttttg attcacgtag
     2521 ttttctgtat tcttaaaata agttggttcc acacatacca atacatgcat gtgctgatta
     2581 taagaattat ctttattatt tattgtcact tccgttgcac gcataaaacc aacaagattt
     2641 ttattaattt ttttatattg catcattcgg cgaaatcctt gagccatatc tgacaaactc
     2701 ttatttaatt cttcgccatc ataaacattt ttaactgtta atgtgagaaa caaccaacga
     2761 actgttggct tttgtttaat aacttcagca acaacctttt gtgactgaat gccatgtttc
     2821 attgctctcc tccagttgca cattggacaa agcctggatt tacaaaacca cactcgatac
     2881 aactttcttt cgcctgtttc acgattttgt ttatactcta atatttcagc acaatctttt
     2941 actctttcag cctttttaaa ttcaagaata tgcagaagtt caaagtaatc aacattagcg
     3001 attttctttt ctctccatgg tctcactttt ccactttttg tcttgtccac taaaaccctt
     3061 gatttttcat ctgaataaat gctactatta ggacacataa tattaaaaga aacccccatc
     3121 tatttagtta tttgtttggt cacttataac tttaacagat ggggtttttc tgtgcaacca
     3181 attttaaggg ttttcaatac tttaaaacac atacatacca acacttcaac gcacctttca
     3241 gcaactaaaa taaaaatgac gttatttcta tatgtatcaa gaatagaaag aactcgtttt
     3301 tcgctacgct caaaacgcaa aaaaagcact cattcgagtg ctttttctta tcgctccaaa
     3361 tcatgcgatt ttttcctctt tgcttttctt tgctcacgaa gttctcgatc acgctgcaaa
     3421 acatcttgaa gcgaaaaagt attcttcttt tcttccgatc gctcatgctg acgcacgaaa
     3481 agccctctag gcgcatagga acaactccta aatgcatgtg aggggttttc tcgtccatgt
     3541 gaacagtcgc atacgcaata ttttgtttcc catagcggcc gcgattatca aaaaggatct
     3601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagc
     3781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4681 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcga taggcgtggc
     4741 ttattgcccc cactgcgacg gaccgctgtt tgcagggaag gatgtggccg taattggcgg
     4801 aggcaattcc ggtatagaag ctgccattga tttggcgggc attgtaagac acgtaacggt
     4861 actggaattt ctgccgcagc tgaaagctga caaagtgctt caggaacgtt tgtacaggct
     4921 tcccaatgta actgtgctta caaatgtcca gacaaaagag tttaccggga aggaaaagct
     4981 cgacgggatt acttacattg agcgtgacac taatcaagaa aagcacattg aggttcaggg
     5041 agtgtttgtt cagattggcc ttgtcccaaa taccgaatgg ctggaaggaa ccattgaacg
     5101 caatgccatg ggtgaaatta ttgtcaatga aaaaaatgaa acctcaatgc ccggggtatt
     5161 tgcagcaggt gactgtacca acagccccta taaacagatt gttatagcca tgggttccgg
     5221 tgcgacggcc gcactcagcg ctttcgatta cctaatcagg aattaattca acaaccagag
     5281 acacttggga aataacatat ccacagagtt atccacatta tccacagctt atttttaaac
     5341 agaatggata tttgcttatt gtgaattgtg gataattttg tggaaaaaat ttttgtgtaa
     5401 ataagtcgaa attggtggta tatatataat atcaaaacaa aactttctac ataggaggga
     5461 tggtcatgtc gaaggttatg aaaaccatgg atggcaatac agccgctgca cacgtagcat
     5521 acgcctttac agaagtagct gccatctatc ctataactcc atcatcacct atggcagagc
     5581 acgtcgatga atggagtgcc cacggcagaa aaaatctctt tgggcaagaa gtgaaagtca
     5641 tcgaaatgca gtcagaagca ggtgctgctg gtgcagtaca cggttctctt gctgctggtg
     5701 cactaactac aacattcaca gcatcacaag gattactatt gatgattcct aatatgtaca
     5761 agatcgctgg tgaacttttg ccaggcgtat tccacgttag cgctcgtgcc cttgcatcac
     5821 atgcgctatc aatatttggt gaccatcaag acgtaatggc atgcagacaa acaggatttg
     5881 cgcttcttgc atcaggcagt gttcaggaag tcatggactt aggctcagtt gcacatttag
     5941 cagctataaa gggtagagtg ccatttttgc atttctttga tggttttagg acatctcacg
     6001 aatatcagaa gatagaagta atggactatg aagatttaag aaagctttta gatatggatg
     6061 cagtaagaga attcaagaag agagcattaa atccagagca tcctgtcaca agaggcacag
     6121 ctcaaaaccc tgacatatac ttccaagaaa gagaagcatc caacaggtac tacaatgctg
     6181 tgccagaaat agtagaagaa tacatgaagg aaataagcaa gattacaggc agagagtaca
     6241 agctatttaa ctactatggt gctcctgacg ctgaacgcat tgtaatcgct atgggttcag
     6301 ttacagagac gattgaggag acaatcgact acttgctgaa aaaaggcgaa aaagtaggtg
     6361 tcgtaaaagt acatctctac aggcctttct catttaaaca cttcatggat gctataccaa
     6421 agacagtaaa gaagatagcc gtattagaca ggacgaaaga agcaggcgca tttggcgaac
     6481 cgctttatga agatgtaaga gcagctttct acgacagtga aatgaaacct attatcgtag
     6541 gagggcgtta cggtttaggc tctaaggaca ctacaccagc tcaaatagtg gcagtatttg
     6601 acaacctaaa gtcagataca ccgaaaaaca acttcacaat tggcatagtg gatgatgtga
     6661 catacacatc gcttcctgtt ggtgaagaaa tcgaaacaac agcagaaggc accataagct
     6721 gcaaattctg gggatttggt tcagacggca cagttggcgc aaacaagagt gctatacaga
     6781 taatcggcga caacactgat atgtatgccc aagcatactt ctcatacgac tctaagaagt
     6841 ctggcggcgt aacaatatcc catttgcgct ttggcaaaaa gccaataagg tcaacgtacc
     6901 ttatcaacaa tgctgatttt gtagcatgcc ataaacaggc ttatgtgtac aactacgatg
     6961 tgcttgcagg cctcaaaaaa ggcggcacat tcttgttaaa ctgcacatgg aaaccagaag
     7021 aactcgatga aaagctacct gcttctatga agaggtacat cgctaagaac aacatcaatt
     7081 tctacatcat aaatgcagtt gacatagcaa aagaattagg tcttggcgca agaataaaca
     7141 tgataatgca atcagcattc tttaagcttg cgaacatcat tcctattgat gaagcagtta
     7201 aacacttaaa agatgctatt gtgaagtctt acggtcataa aggcgaaaag atagtcaaca
     7261 tgaactacgc agccgtcgat cgtggaatag atgcacttgt taaggtagat gttcctgcat
     7321 cgtgggcaaa tgctgaagac gaagctaaag tagaaagaaa tgttcctgat ttcataaaga
     7381 atatagcaga tgttatgaat agacaagaag gagacaaatt gcctgtaagc gcctttgtag
     7441 gcatggaaga cggtacattc ccaatgggca ctgcagcata cgagaagcgc ggtatcgccg
     7501 tagatgtgcc tgaatggcag atagataact gcatacagtg caatcagtgt gcgtatgtat
     7561 gtccacatgc agcaatcagg ccattcctct taaatgaaga agaagtcaaa aacgctcctg
     7621 aaggatttac ttccaaaaaa gctatcggta agggcttaga aggattgaac ttcaggattc
     7681 aagtgagcgt gcttgattgt acaggctgtg gcgtatgcgc aaacacatgt ccttcaaaag
     7741 aaaagtctct cataatgaag cctttagaga cacagttaga tcaagcaaag aattgggaat
     7801 acgctatgtc attatcatac aaggagaatc cattaggaac agatacggta aagggcagcc
     7861 aatttgagaa gcctttgctt gaattctctg gtgcttgcgc aggatgcggc gaaacaccat
     7921 acgcaaggct tgtaacacaa ttatttggcg atagaatgct tatcgcaaat gctacaggtt
     7981 gctcatccat ctggggtggc agcgcaccat ctacaccgta cacagtaaac aaagatggtc
     8041 atggtccagc ttgggctaac tcactgtttg aagacaacgc tgagtttggc ttcggtatgg
     8101 cattagctgt aaaacaacag agagaaaaat tggctgatat agtaaaagaa gcattagaat
     8161 tagatcttac gcaagattta aagaatgcgt taaagttatg gcttgacaat ttcaatagct
     8221 ctgagataac aaagaagaca gccaatatca tagtttcatt gatacaggac tacaagacag
     8281 atgactcaaa agtcaaagaa cttttaaatg aaatacttga taggaaagaa tatcttgtca
     8341 agaagtcaca gtggatattt ggcggtgacg gttgggcata cgatataggc tttggtggtc
     8401 ttgaccatgt gctggcatcc ggtgaagacg taaatgttct tgtatttgat acagaagtgt
     8461 attcaaacac aggtggtcaa tcatcaaagg cgacaccagt aggcgctata gcacaatttg
     8521 ctgcagcagg taaaggaatc ggcaagaagg atttaggacg cattgcaatg agctatggat
     8581 atgtttatgt tgcgcagata gctatgggcg ctaaccaagc acagacaata aaagccttga
     8641 aagaggcaga aagctatcct ggcccatcgc ttataatagc atatgctcca tgtatcaacc
     8701 atggcatcaa gcttggaatg ggttgcagcc agatagaaga aaagaaagca gttgaagctg
     8761 gatactggca cctatacagg tacaatccaa tgcttaaagc tgagggcaag aatccattca
     8821 tccttgactc aaaggctcca acagcttcat acaaagaatt catcatggga gaagttcgct
     8881 attcttcact ggcaaagaca ttcccagaaa gagcagaagc tttgtttgaa aaagctgaag
     8941 aacttgcaaa agaaaaatac gaaacataca agaagcttgc tgaacaaaat tagtaaagga
     9001 ggcgatatta atggcacata ttattacaga cgaatgcata agctgtggtg cttgcgctgc
     9061 agaatgccct gtagatgcta ttcacgaagg tacaggaaaa tacgaagtgg atgctgatac
     9121 atgtatagat tgtggtgcat gtgaaccagt atgcccaaca ggagctatta aggctgaata
     9181 agaaattaac agaggacggt tctttgtttg atatcaaaca gggaaccgtc caaactattg
     9241 gtgaaagtat aatagataga tttttacctt gaaaaatcat agtatttgtc tgtacgatac
     9301 caattccaaa aattttgtcc ttgtgtcatg agaagctggt ctaaaaatac ttcaaaatta
     9361 tgtccaagac atttaaactc atcgcgcaaa atatttggtc cgacatacaa ataattgttg
     9421 gtattggctt ctttgctgtt gattactata taaatttccg ttttggattc ttttgattgc
     9481 ggagattata tcttgcatgc tcaagtaaat caactcccac ataataattt ttttattatt
     9541 tactcctgct ttaggcaaaa gaacaaaatt taacatcgac aaattaacat agttgtttat
     9601 ttgtatcaag cagaaaaaaa tatgtatatg atgccggccg accgacacaa aaatgtgagc
     9661 gttcacgaaa caataaatat ataaataagg gtattaattc tgcaaaaaga aaagtgtttg
     9721 ctacatgagg tccattaatt tatcgtggga ataggcatgg aaaagatttt tttgcccgag
     9781 gatgcattga taaagtattt cttttccgaa agagaggaaa agattctaaa gagttttgga
     9841 aatactgatg aatattgtgt gcagagtaca attctatgga caagaaaaga ggctttgtca
     9901 aaactttttc gtctgggaat gaggatggat tttaaaaagc tggatacttt ggagggcgag
     9961 gtggtttttc aggaaacaaa cagggcgcgt ctgttttctt ttatatgcaa taattactgt
    10021 atctctctgg cattgccagg ttttaataaa gattaaaatt attgactaga aataaaaaaa
    10081 ttgtccataa tattaatgga caaaaaaaca aagaattaca tcaaaggaag ataaaaatac
    10141 tttgttaaaa aattaattat tttttatcta aactattgaa aatgaaaata aaataatata
    10201 aaatgaatca tagtgcaaga gatacttgcc agaggatgaa tattttactg cattcatgct
    10261 ttatggcagc taatagaggc attaaattaa attttaattt acaataggag gcgatattaa
    10321 tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
    10381 tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
    10441 acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
    10501 taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
    10561 ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
    10621 ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
    10681 aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
    10741 tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
    10801 attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
    10861 taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
    10921 tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa taaaggaggt
    10981 cgacgtcatg gaaaatttat caaaagacat cgatgaaatt ttgatcacag aagaagaact
    11041 taaggaaaag ataaaagagc ttgggaggca aatcacaaaa gactacaaag ggaaaaattt
    11101 gatgttggta ggagttttaa aaggtgcttt aatgtttatg gctgatttgt caagacacat
    11161 agatttgcct ttatcacttg attttatggc tgtttccagc tatggaagct caactcattc
    11221 atcaggaata gtaaagataa tcaaagatct tgatataagc atagaaggca aagatgttct
    11281 gattgtggaa gacataattg acagcggttt gactttgtct tacttaaggg aaactttact
    11341 tggaaggaag ccaaaaagcc tgaaaatatg cacaatatta gacaaaccgg agagaagaga
    11401 agcatctgta aaagtcgatt atgtaggatt taagatacct gataagtttg tcgtgggtta
    11461 tggattggac tttgatgaaa agtacaggaa ccttcctttt ataggcgttt tgaaacctga
    11521 aatgtacagc taagcccggg cctcgagaaa acaaaaggct cagtcggaag actgggcctt
    11581 ttgttttggt accgaattcg gcgcgcctca gcgtttaaac ccgctgatcc tagagggccg
    11641 catcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccatgctt taatacatac
    11701 ctggcccagt agttcaggaa attaacagag gacggttctt tgtttgatat caaacaggga
    11761 accgtccaaa ctattggtga aagtataata gatagatttt taccttgaaa aatcatagta
    11821 tttgtctgta cgataccaat tccaaaaatt ttgtccttgt gtcatgagaa gctggtctaa
    11881 aaatacttca aaattatgtc caagacattt aaactcatcg cgcaaaatat ttggtccgac
    11941 atacaaataa ttgttggtat tggcttcttt gctgttgatt actatataaa tttccgtttt
    12001 ggattctttt gattgcggag attatatctt gcatgctcaa gtaaatcaac tcccacataa
    12061 taattttttt attatttact cctgctttag gcaaaagaac aaaatttaac atcgacaaat
    12121 taacatagtt gtttatttgt atcaagcaga aaaaaatatg tatatgatgc cggccgaccg
    12181 acacaaaaat gtgagcgttc acgaaacaat aaatatataa ataagggtat taattctgca
    12241 aaaagaaaag tgtttgctac atgaggtcca ttaatttctg ctaatagtag tgaaaaaatc
    12301 agggataatg ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
    12361 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
    12421 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
    12481 gttttgggac gctcgaaggc tttaatttgc aagctgcggc
//
