LOCUS       V003242                12584 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003242
VERSION     V003242
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Hon S, Holwerda EK, Worthen RS, Maloney MI, Tian L, Cui J, Lin PP,
            Lynd LR, Olson DG.
  TITLE     Expressing the Thermoanaerobacterium saccharolyticum pforA in
            engineered Clostridium thermocellum improves ethanol production
  JOURNAL   Biotechnol Biofuels 11, 242 (2018)
   PUBMED   30202437
REFERENCE   2  (bases 1 to 12584)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Worthen RS, Maloney MI, Tian L, Cui J,
            Lin PP, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 12584)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12584)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 11, 242 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-APR-2018) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..573
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(840..1418)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label="tdk"
                     /protein_id="AYA22175.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(840..1418)
                     /gene="tdk"
                     /label="tdk"
     regulatory      complement(1419..2039)
                     /label="C. thermocellum cbp promoter"
                     /note="C. thermocellum cbp promoter"
                     /regulatory_class="promoter"
     CDS             complement(2118..3119)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(3120..3574)
                     /direction=LEFT
                     /label="C. thermocellum origin of replication"
                     /note="C. thermocellum origin of replication"
     CDS             complement(3680..4537)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4538..4642)
                     /label="AmpR promoter"
     misc_feature    4732..5266
                     /label="upstream homology recombination flank"
                     /note="upstream homology recombination flank"
     regulatory      5267..5529
                     /label="T. saccharolyticum pforA promoter"
                     /note="T. saccharolyticum pforA promoter"
                     /regulatory_class="promoter"
     CDS             5530..9057
                     /codon_start=1
                     /gene="pforA"
                     /product="pyruvate ferredoxin oxidoreductase"
                     /label="pforA"
                     /note="derived from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AYA22178.1"
                     /translation="MSKVMKTMDGNTAAAHVAYAFTEVAAIYPITPSSPMAEHVDEWSA
                     HGRKNLFGQEVKVIEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMYKIAGEL
                     LPGVFHVSARALASHALSIFGDHQDVMACRQTGFALLASGSVQEVMDLGSVAHLAAIKG
                     RVPFLHFFDGFRTSHEYQKIEVMDYEDLRKLLDMDAVREFKKRALNPEHPVTRGTAQNP
                     DIYFQEREASNRYYNAVPEIVEEYMKEISKITGREYKLFNYYGAPDAERIVIAMGSVTE
                     TIEETIDYLLKKGEKVGVVKVHLYRPFSFKHFMDAIPKTVKKIAVLDRTKEAGAFGEPL
                     YEDVRAAFYDSEMKPIIVGGRYGLGSKDTTPAQIVAVFDNLKSDTPKNNFTIGIVDDVT
                     YTSLPVGEEIETTAEGTISCKFWGFGSDGTVGANKSAIQIIGDNTDMYAQAYFSYDSKK
                     SGGVTISHLRFGKKPIRSTYLINNADFVACHKQAYVYNYDVLAGLKKGGTFLLNCTWKP
                     EELDEKLPASMKRYIAKNNINFYIINAVDIAKELGLGARINMIMQSAFFKLANIIPIDE
                     AVKHLKDAIVKSYGHKGEKIVNMNYAAVDRGIDALVKVDVPASWANAEDEAKVERNVPD
                     FIKNIADVMNRQEGDKLPVSAFVGMEDGTFPMGTAAYEKRGIAVDVPEWQIDNCIQCNQ
                     CAYVCPHAAIRPFLLNEEEVKNAPEGFTSKKAIGKGLEGLNFRIQVSVLDCTGCGVCAN
                     TCPSKEKSLIMKPLETQLDQAKNWEYAMSLSYKENPLGTDTVKGSQFEKPLLEFSGACA
                     GCGETPYARLVTQLFGDRMLIANATGCSSIWGGSAPSTPYTVNKDGHGPAWANSLFEDN
                     AEFGFGMALAVKQQREKLADIVKEALELDLTQDLKNALKLWLDNFNSSEITKKTANIIV
                     SLIQDYKTDDSKVKELLNEILDRKEYLVKKSQWIFGGDGWAYDIGFGGLDHVLASGEDV
                     NVLVFDTEVYSNTGGQSSKATPVGAIAQFAAAGKGIGKKDLGRIAMSYGYVYVAQIAMG
                     ANQAQTIKALKEAESYPGPSLIIAYAPCINHGIKLGMGCSQIEEKKAVEAGYWHLYRYN
                     PMLKAEGKNPFILDSKAPTASYKEFIMGEVRYSSLAKTFPERAEALFEKAEELAKEKYE
                     TYKKLAEQN"
     gene            5530..9057
                     /gene="pforA"
                     /label="pforA"
                     /note="Tsac_0046"
     CDS             9075..9245
                     /codon_start=1
                     /gene="Tsac_2084"
                     /product="ferredoxin"
                     /label="Tsac_2084"
                     /note="derived from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AYA22179.1"
                     /translation="MAHIITDECISCGACAAECPVDAIHEGTGKYEVDADTCIDCGACE
                     PVCPTGAIKAE"
     gene            9075..9245
                     /gene="Tsac_2084"
                     /label="Tsac_2084"
     misc_feature    9246..9805
                     /label="first downstream homology recombination flank"
                     /note="first downstream homology recombination flank"
     regulatory      9809..10383
                     /label="C. thermocellum gapDH promoter"
                     /note="C. thermocellum gapDH promoter"
                     /regulatory_class="promoter"
     CDS             10384..11031
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             11052..11597
                     /codon_start=1
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /protein_id="AYA22181.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            11052..11597
                     /gene="hpt"
                     /label="hpt"
     misc_feature    11782..12341
                     /label="second downstream homology recombination flank"
                     /note="second downstream homology recombination flank"
     terminator      12374..12562
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ttttatctga ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg
       61 ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa
      121 ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt
      181 ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg
      241 ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
      361 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
      421 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac
      481 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
      541 tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt
      601 ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt
      661 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata
      721 tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag
      781 cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacaaa aaaatccgct
      841 taagtccgcg ggacttcatg gcactttcta caccttgctt cataagactc ctttgctcca
      901 accattataa tgggatcgtc gtaattggca ggcttcccat ttataagcct ttgcgtgcga
      961 gtagcaggat tgccacactt catgcatata gcagtaagct tatcgacaaa ttcagctatg
     1021 gccatcaatt ctggagtcgg accaaatggt tcacctctaa agtccatgtc aagccctgca
     1081 catatgactc ttttcccgct atcggcaatc tcttttacga tgtcgactat ttcagaatca
     1141 aaaaattgaa cttcatctat agcaaataca tccgtatctt cttcagcata agccaatatg
     1201 tcagaagcct ttactatggc aatggcgtgc atgttgtcgc cgttatgaga tacgactttg
     1261 tctatggaat acctatcgtc tatagccggt ttgaaaacct gaactttttg cctggcaatc
     1321 ttagctctct ttatccttct tataagttcc tcactttttc cactgaacat gggaccagtt
     1381 acaacttcta tgtacccgtg gtctttaggc ccatacatct ttcgtcctcc ttaaaatttt
     1441 cgtttatttg ttttttattt agtgtgccta aaagcaccat cttatacatt cgtaccttat
     1501 atctcttatc ttgtatctct tacctcatat ctcttattat taactaccga agtatatttt
     1561 cataacggaa tcttatgact tatttttctg actgccgttt taccatacgg ttaatttata
     1621 cggaaacagc taatctaacc ttgctgaagg catgtttacc aaaatattat atctacttta
     1681 ccatattaat tcaagtggtc attttgatat tttacgccaa aatttttagc gttcctttac
     1741 ttgataattt atccaaaaac cttcattcgt caaaacaatt atgatataat taatttatga
     1801 tatgattaat taatatttac ggataactaa tcatttcaat gcaatagatc aacatgcaaa
     1861 tcaatatcca ccttactccg aacattttgc accaacgaat gcccagcact atatcccggc
     1921 aagcaatata acgtctataa ttttttatga taacgataaa attaaatttt atgaaagggg
     1981 taaaagcatg agaaaaatag ctgtattgtt aattactttg acgttcttag tcacgactcg
     2041 aatttaggag gcttacttgt ctgctttctt cattagaatc aatccttttt taaaagtcaa
     2101 tcccgtttgt tgaactactc tttaataaaa taatttttcc gttcccaatt ccacattgca
     2161 ataatagaaa atccatcttc atcggctttt tcgtcatcat ctgtatgaat caaatcgcct
     2221 tcttctgtgt catcaaggtt taatttttta tgtatttctt ttaacaaacc accataggag
     2281 attaaccttt tacggtgtaa accttcctcc aaatcagaca aacgtttcaa attcttttct
     2341 tcatcatcgg tcataaaatc cgtatccttt acaggatatt ttgcagtttc gtcaattgcc
     2401 gattgtatat ccgatttata tttatttttc ggtcgaatca tttgaacttt tacatttgga
     2461 tcatagtcta atttcattgc ctttttccaa aattgaatcc attgtttttg attcacgtag
     2521 ttttctgtat tcttaaaata agttggttcc acacatacca atacatgcat gtgctgatta
     2581 taagaattat ctttattatt tattgtcact tccgttgcac gcataaaacc aacaagattt
     2641 ttattaattt ttttatattg catcattcgg cgaaatcctt gagccatatc tgacaaactc
     2701 ttatttaatt cttcgccatc ataaacattt ttaactgtta atgtgagaaa caaccaacga
     2761 actgttggct tttgtttaat aacttcagca acaacctttt gtgactgaat gccatgtttc
     2821 attgctctcc tccagttgca cattggacaa agcctggatt tacaaaacca cactcgatac
     2881 aactttcttt cgcctgtttc acgattttgt ttatactcta atatttcagc acaatctttt
     2941 actctttcag cctttttaaa ttcaagaata tgcagaagtt caaagtaatc aacattagcg
     3001 attttctttt ctctccatgg tctcactttt ccactttttg tcttgtccac taaaaccctt
     3061 gatttttcat ctgaataaat gctactatta ggacacataa tattaaaaga aacccccatc
     3121 tatttagtta tttgtttggt cacttataac tttaacagat ggggtttttc tgtgcaacca
     3181 attttaaggg ttttcaatac tttaaaacac atacatacca acacttcaac gcacctttca
     3241 gcaactaaaa taaaaatgac gttatttcta tatgtatcaa gaatagaaag aactcgtttt
     3301 tcgctacgct caaaacgcaa aaaaagcact cattcgagtg ctttttctta tcgctccaaa
     3361 tcatgcgatt ttttcctctt tgcttttctt tgctcacgaa gttctcgatc acgctgcaaa
     3421 acatcttgaa gcgaaaaagt attcttcttt tcttccgatc gctcatgctg acgcacgaaa
     3481 agccctctag gcgcatagga acaactccta aatgcatgtg aggggttttc tcgtccatgt
     3541 gaacagtcgc atacgcaata ttttgtttcc catagcggcc gcgattatca aaaaggatct
     3601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagc
     3781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4681 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcga taggcgtggc
     4741 ttattgcccc cactgcgacg gaccgctgtt tgcagggaag gatgtggccg taattggcgg
     4801 aggcaattcc ggtatagaag ctgccattga tttggcgggc attgtaagac acgtaacggt
     4861 actggaattt ctgccgcagc tgaaagctga caaagtgctt caggaacgtt tgtacaggct
     4921 tcccaatgta actgtgctta caaatgtcca gacaaaagag tttaccggga aggaaaagct
     4981 cgacgggatt acttacattg agcgtgacac taatcaagaa aagcacattg aggttcaggg
     5041 agtgtttgtt cagattggcc ttgtcccaaa taccgaatgg ctggaaggaa ccattgaacg
     5101 caatgccatg ggtgaaatta ttgtcaatga aaaaaatgaa acctcaatgc ccggggtatt
     5161 tgcagcaggt gactgtacca acagccccta taaacagatt gttatagcca tgggttccgg
     5221 tgcgacggcc gcactcagcg ctttcgatta cctaatcagg aattaaatca cataatatga
     5281 ccagctcata atttttatga ataaattttt gtcaaaaaaa tatttttata ttttaattat
     5341 ctgtgatata attatcgtgg aactgttata taaagtctaa aggtatatat ataagcattt
     5401 tcagaaaact gttatataat cgcttaaccg ctgttgtata acagtacata tttgtttatg
     5461 ctgtattata atagtttgct tatatatcct tagatttaaa tataaattta aaaagagagg
     5521 ggaaatacaa tgtcgaaggt tatgaaaacc atggatggca atacagccgc tgcacacgta
     5581 gcatacgcct ttacagaagt agctgccatc tatcctataa ctccatcatc acctatggca
     5641 gagcacgtcg atgaatggag tgcccacggc agaaaaaatc tctttgggca agaagtgaaa
     5701 gtcatcgaaa tgcagtcaga agcaggtgct gctggtgcag tacacggttc tcttgctgct
     5761 ggtgcactaa ctacaacatt cacagcatca caaggattac tattgatgat tcctaatatg
     5821 tacaagatcg ctggtgaact tttgccaggc gtattccacg ttagcgctcg tgcccttgca
     5881 tcacatgcgc tatcaatatt tggtgaccat caagacgtaa tggcatgcag acaaacagga
     5941 tttgcgcttc ttgcatcagg cagtgttcag gaagtcatgg acttaggctc agttgcacat
     6001 ttagcagcta taaagggtag agtgccattt ttgcatttct ttgatggttt taggacatct
     6061 cacgaatatc agaagataga agtaatggac tatgaagatt taagaaagct tttagatatg
     6121 gatgcagtaa gagaattcaa gaagagagca ttaaatccag agcatcctgt cacaagaggc
     6181 acagctcaaa accctgacat atacttccaa gaaagagaag catccaacag gtactacaat
     6241 gctgtgccag aaatagtaga agaatacatg aaggaaataa gcaagattac aggcagagag
     6301 tacaagctat ttaactacta tggtgctcct gacgctgaac gcattgtaat cgctatgggt
     6361 tcagttacag agacgattga ggagacaatc gactacttgc tgaaaaaagg cgaaaaagta
     6421 ggtgtcgtaa aagtacatct ctacaggcct ttctcattta aacacttcat ggatgctata
     6481 ccaaagacag taaagaagat agccgtatta gacaggacga aagaagcagg cgcatttggc
     6541 gaaccgcttt atgaagatgt aagagcagct ttctacgaca gtgaaatgaa acctattatc
     6601 gtaggagggc gttacggttt aggctctaag gacactacac cagctcaaat agtggcagta
     6661 tttgacaacc taaagtcaga tacaccgaaa aacaacttca caattggcat agtggatgat
     6721 gtgacataca catcgcttcc tgttggtgaa gaaatcgaaa caacagcaga aggcaccata
     6781 agctgcaaat tctggggatt tggttcagac ggcacagttg gcgcaaacaa gagtgctata
     6841 cagataatcg gcgacaacac tgatatgtat gcccaagcat acttctcata cgactctaag
     6901 aagtctggcg gcgtaacaat atcccatttg cgctttggca aaaagccaat aaggtcaacg
     6961 taccttatca acaatgctga ttttgtagca tgccataaac aggcttatgt gtacaactac
     7021 gatgtgcttg caggcctcaa aaaaggcggc acattcttgt taaactgcac atggaaacca
     7081 gaagaactcg atgaaaagct acctgcttct atgaagaggt acatcgctaa gaacaacatc
     7141 aatttctaca tcataaatgc agttgacata gcaaaagaat taggtcttgg cgcaagaata
     7201 aacatgataa tgcaatcagc attctttaag cttgcgaaca tcattcctat tgatgaagca
     7261 gttaaacact taaaagatgc tattgtgaag tcttacggtc ataaaggcga aaagatagtc
     7321 aacatgaact acgcagccgt cgatcgtgga atagatgcac ttgttaaggt agatgttcct
     7381 gcatcgtggg caaatgctga agacgaagct aaagtagaaa gaaatgttcc tgatttcata
     7441 aagaatatag cagatgttat gaatagacaa gaaggagaca aattgcctgt aagcgccttt
     7501 gtaggcatgg aagacggtac attcccaatg ggcactgcag catacgagaa gcgcggtatc
     7561 gccgtagatg tgcctgaatg gcagatagat aactgcatac agtgcaatca gtgtgcgtat
     7621 gtatgtccac atgcagcaat caggccattc ctcttaaatg aagaagaagt caaaaacgct
     7681 cctgaaggat ttacttccaa aaaagctatc ggtaagggct tagaaggatt gaacttcagg
     7741 attcaagtga gcgtgcttga ttgtacaggc tgtggcgtat gcgcaaacac atgtccttca
     7801 aaagaaaagt ctctcataat gaagccttta gagacacagt tagatcaagc aaagaattgg
     7861 gaatacgcta tgtcattatc atacaaggag aatccattag gaacagatac ggtaaagggc
     7921 agccaatttg agaagccttt gcttgaattc tctggtgctt gcgcaggatg cggcgaaaca
     7981 ccatacgcaa ggcttgtaac acaattattt ggcgatagaa tgcttatcgc aaatgctaca
     8041 ggttgctcat ccatctgggg tggcagcgca ccatctacac cgtacacagt aaacaaagat
     8101 ggtcatggtc cagcttgggc taactcactg tttgaagaca acgctgagtt tggcttcggt
     8161 atggcattag ctgtaaaaca acagagagaa aaattggctg atatagtaaa agaagcatta
     8221 gaattagatc ttacgcaaga tttaaagaat gcgttaaagt tatggcttga caatttcaat
     8281 agctctgaga taacaaagaa gacagccaat atcatagttt cattgataca ggactacaag
     8341 acagatgact caaaagtcaa agaactttta aatgaaatac ttgataggaa agaatatctt
     8401 gtcaagaagt cacagtggat atttggcggt gacggttggg catacgatat aggctttggt
     8461 ggtcttgacc atgtgctggc atccggtgaa gacgtaaatg ttcttgtatt tgatacagaa
     8521 gtgtattcaa acacaggtgg tcaatcatca aaggcgacac cagtaggcgc tatagcacaa
     8581 tttgctgcag caggtaaagg aatcggcaag aaggatttag gacgcattgc aatgagctat
     8641 ggatatgttt atgttgcgca gatagctatg ggcgctaacc aagcacagac aataaaagcc
     8701 ttgaaagagg cagaaagcta tcctggccca tcgcttataa tagcatatgc tccatgtatc
     8761 aaccatggca tcaagcttgg aatgggttgc agccagatag aagaaaagaa agcagttgaa
     8821 gctggatact ggcacctata caggtacaat ccaatgctta aagctgaggg caagaatcca
     8881 ttcatccttg actcaaaggc tccaacagct tcatacaaag aattcatcat gggagaagtt
     8941 cgctattctt cactggcaaa gacattccca gaaagagcag aagctttgtt tgaaaaagct
     9001 gaagaacttg caaaagaaaa atacgaaaca tacaagaagc ttgctgaaca aaattagtaa
     9061 aggaggcgat attaatggca catattatta cagacgaatg cataagctgt ggtgcttgcg
     9121 ctgcagaatg ccctgtagat gctattcacg aaggtacagg aaaatacgaa gtggatgctg
     9181 atacatgtat agattgtggt gcatgtgaac cagtatgccc aacaggagct attaaggctg
     9241 aataagaaat taacagagga cggttctttg tttgatatca aacagggaac cgtccaaact
     9301 attggtgaaa gtataataga tagattttta ccttgaaaaa tcatagtatt tgtctgtacg
     9361 ataccaattc caaaaatttt gtccttgtgt catgagaagc tggtctaaaa atacttcaaa
     9421 attatgtcca agacatttaa actcatcgcg caaaatattt ggtccgacat acaaataatt
     9481 gttggtattg gcttctttgc tgttgattac tatataaatt tccgttttgg attcttttga
     9541 ttgcggagat tatatcttgc atgctcaagt aaatcaactc ccacataata atttttttat
     9601 tatttactcc tgctttaggc aaaagaacaa aatttaacat cgacaaatta acatagttgt
     9661 ttatttgtat caagcagaaa aaaatatgta tatgatgccg gccgaccgac acaaaaatgt
     9721 gagcgttcac gaaacaataa atatataaat aagggtatta attctgcaaa aagaaaagtg
     9781 tttgctacat gaggtccatt aatttatcgt gggaataggc atggaaaaga tttttttgcc
     9841 cgaggatgca ttgataaagt atttcttttc cgaaagagag gaaaagattc taaagagttt
     9901 tggaaatact gatgaatatt gtgtgcagag tacaattcta tggacaagaa aagaggcttt
     9961 gtcaaaactt tttcgtctgg gaatgaggat ggattttaaa aagctggata ctttggaggg
    10021 cgaggtggtt tttcaggaaa caaacagggc gcgtctgttt tcttttatat gcaataatta
    10081 ctgtatctct ctggcattgc caggttttaa taaagattaa aattattgac tagaaataaa
    10141 aaaattgtcc ataatattaa tggacaaaaa aacaaagaat tacatcaaag gaagataaaa
    10201 atactttgtt aaaaaattaa ttatttttta tctaaactat tgaaaatgaa aataaaataa
    10261 tataaaatga atcatagtgc aagagatact tgccagagga tgaatatttt actgcattca
    10321 tgctttatgg cagctaatag aggcattaaa ttaaatttta atttacaata ggaggcgata
    10381 ttaatgaact ttaataaaat tgatttagac aattggaaga gaaaagagat atttaatcat
    10441 tatttgaacc aacaaacgac ttttagtata accacagaaa ttgatattag tgttttatac
    10501 cgaaacataa aacaagaagg atataaattt taccctgcat ttattttctt agtgacaagg
    10561 gtgataaact caaatacagc ttttagaact ggttacaata gcgacggaga gttaggttat
    10621 tgggataagt tagagccact ttatacaatt tttgatggtg tatctaaaac attctctggt
    10681 atttggactc ctgtaaagaa tgacttcaaa gagttttatg atttatacct ttctgatgta
    10741 gagaaatata atggttcggg gaaattgttt cccaaaacac ctatacctga aaatgctttt
    10801 tctctttcta ttattccatg gacttcattt actgggttta acttaaatat caataataat
    10861 agtaattacc ttctacccat tattacagca ggaaaattca ttaataaagg taattcaata
    10921 tatttaccgc tatctttaca ggtacatcat tctgtttgtg atggttatca tgcaggattg
    10981 tttatgaact ctattcagga attgtcagat aggcctaatg actggctttt ataataaagg
    11041 aggtcgacgt catggaaaat ttatcaaaag acatcgatga aattttgatc acagaagaag
    11101 aacttaagga aaagataaaa gagcttggga ggcaaatcac aaaagactac aaagggaaaa
    11161 atttgatgtt ggtaggagtt ttaaaaggtg ctttaatgtt tatggctgat ttgtcaagac
    11221 acatagattt gcctttatca cttgatttta tggctgtttc cagctatgga agctcaactc
    11281 attcatcagg aatagtaaag ataatcaaag atcttgatat aagcatagaa ggcaaagatg
    11341 ttctgattgt ggaagacata attgacagcg gtttgacttt gtcttactta agggaaactt
    11401 tacttggaag gaagccaaaa agcctgaaaa tatgcacaat attagacaaa ccggagagaa
    11461 gagaagcatc tgtaaaagtc gattatgtag gatttaagat acctgataag tttgtcgtgg
    11521 gttatggatt ggactttgat gaaaagtaca ggaaccttcc ttttataggc gttttgaaac
    11581 ctgaaatgta cagctaagcc cgggcctcga gaaaacaaaa ggctcagtcg gaagactggg
    11641 ccttttgttt tggtaccgaa ttcggcgcgc ctcagcgttt aaacccgctg atcctagagg
    11701 gccgcatcat gtaattagtt atgtcacgct tacattcacg ccctccccat gctttaatac
    11761 atacctggcc cagtagttca ggaaattaac agaggacggt tctttgtttg atatcaaaca
    11821 gggaaccgtc caaactattg gtgaaagtat aatagataga tttttacctt gaaaaatcat
    11881 agtatttgtc tgtacgatac caattccaaa aattttgtcc ttgtgtcatg agaagctggt
    11941 ctaaaaatac ttcaaaatta tgtccaagac atttaaactc atcgcgcaaa atatttggtc
    12001 cgacatacaa ataattgttg gtattggctt ctttgctgtt gattactata taaatttccg
    12061 ttttggattc ttttgattgc ggagattata tcttgcatgc tcaagtaaat caactcccac
    12121 ataataattt ttttattatt tactcctgct ttaggcaaaa gaacaaaatt taacatcgac
    12181 aaattaacat agttgtttat ttgtatcaag cagaaaaaaa tatgtatatg atgccggccg
    12241 accgacacaa aaatgtgagc gttcacgaaa caataaatat ataaataagg gtattaattc
    12301 tgcaaaaaga aaagtgtttg ctacatgagg tccattaatt tctgctaata gtagtgaaaa
    12361 aatcagggat aatgccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
    12421 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
    12481 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
    12541 gaaggttttg ggacgctcga aggctttaat ttgcaagctg cggc
//
