LOCUS       V003243                12499 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003243
VERSION     V003243
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Hon S, Holwerda EK, Worthen RS, Maloney MI, Tian L, Cui J, Lin PP,
            Lynd LR, Olson DG.
  TITLE     Expressing the Thermoanaerobacterium saccharolyticum pforA in
            engineered Clostridium thermocellum improves ethanol production
  JOURNAL   Biotechnol Biofuels 11, 242 (2018)
   PUBMED   30202437
REFERENCE   2  (bases 1 to 12499)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Worthen RS, Maloney MI, Tian L, Cui J,
            Lin PP, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2018) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 12499)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12499)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 11, 242 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-APR-2018) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..573
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(840..1418)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label="tdk"
                     /protein_id="AYA22168.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(840..1418)
                     /gene="tdk"
                     /label="tdk"
     regulatory      complement(1419..2039)
                     /label="C. thermocellum cbp promoter"
                     /note="C. thermocellum cbp promoter"
                     /regulatory_class="promoter"
     CDS             complement(2118..3119)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(3120..3574)
                     /direction=LEFT
                     /label="C. thermocellum origin of replication"
                     /note="C. thermocellum origin of replication"
     CDS             complement(3680..4537)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4538..4642)
                     /label="AmpR promoter"
     misc_feature    4732..5266
                     /label="upstream homology recombination flank"
                     /note="upstream homology recombination flank"
     regulatory      5267..5444
                     /label="C. thermocellum enolase promoter"
                     /note="C. thermocellum enolase promoter"
                     /regulatory_class="promoter"
     CDS             5445..8972
                     /codon_start=1
                     /gene="pforA"
                     /product="pyruvate ferredoxin oxidoreductase"
                     /label="pforA"
                     /note="derived from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AYA22171.1"
                     /translation="MSKVMKTMDGNTAAAHVAYAFTEVAAIYPITPSSPMAEHVDEWSA
                     HGRKNLFGQEVKVIEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMYKIAGEL
                     LPGVFHVSARALASHALSIFGDHQDVMACRQTGFALLASGSVQEVMDLGSVAHLAAIKG
                     RVPFLHFFDGFRTSHEYQKIEVMDYEDLRKLLDMDAVREFKKRALNPEHPVTRGTAQNP
                     DIYFQEREASNRYYNAVPEIVEEYMKEISKITGREYKLFNYYGAPDAERIVIAMGSVTE
                     TIEETIDYLLKKGEKVGVVKVHLYRPFSFKHFMDAIPKTVKKIAVLDRTKEAGAFGEPL
                     YEDVRAAFYDSEMKPIIVGGRYGLGSKDTTPAQIVAVFDNLKSDTPKNNFTIGIVDDVT
                     YTSLPVGEEIETTAEGTISCKFWGFGSDGTVGANKSAIQIIGDNTDMYAQAYFSYDSKK
                     SGGVTISHLRFGKKPIRSTYLINNADFVACHKQAYVYNYDVLAGLKKGGTFLLNCTWKP
                     EELDEKLPASMKRYIAKNNINFYIINAVDIAKELGLGARINMIMQSAFFKLANIIPIDE
                     AVKHLKDAIVKSYGHKGEKIVNMNYAAVDRGIDALVKVDVPASWANAEDEAKVERNVPD
                     FIKNIADVMNRQEGDKLPVSAFVGMEDGTFPMGTAAYEKRGIAVDVPEWQIDNCIQCNQ
                     CAYVCPHAAIRPFLLNEEEVKNAPEGFTSKKAIGKGLEGLNFRIQVSVLDCTGCGVCAN
                     TCPSKEKSLIMKPLETQLDQAKNWEYAMSLSYKENPLGTDTVKGSQFEKPLLEFSGACA
                     GCGETPYARLVTQLFGDRMLIANATGCSSIWGGSAPSTPYTVNKDGHGPAWANSLFEDN
                     AEFGFGMALAVKQQREKLADIVKEALELDLTQDLKNALKLWLDNFNSSEITKKTANIIV
                     SLIQDYKTDDSKVKELLNEILDRKEYLVKKSQWIFGGDGWAYDIGFGGLDHVLASGEDV
                     NVLVFDTEVYSNTGGQSSKATPVGAIAQFAAAGKGIGKKDLGRIAMSYGYVYVAQIAMG
                     ANQAQTIKALKEAESYPGPSLIIAYAPCINHGIKLGMGCSQIEEKKAVEAGYWHLYRYN
                     PMLKAEGKNPFILDSKAPTASYKEFIMGEVRYSSLAKTFPERAEALFEKAEELAKEKYE
                     TYKKLAEQN"
     gene            5445..8972
                     /gene="pforA"
                     /label="pforA"
                     /note="Tsac_0046"
     CDS             8990..9160
                     /codon_start=1
                     /gene="Tsac_2084"
                     /product="ferredoxin"
                     /label="Tsac_2084"
                     /note="derived from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AYA22172.1"
                     /translation="MAHIITDECISCGACAAECPVDAIHEGTGKYEVDADTCIDCGACE
                     PVCPTGAIKAE"
     gene            8990..9160
                     /gene="Tsac_2084"
                     /label="Tsac_2084"
     misc_feature    9161..9720
                     /label="first downstream homology recombination flank"
                     /note="first downstream homology recombination flank"
     regulatory      9724..10298
                     /label="C. thermocellum gapDH promoter"
                     /note="C. thermocellum gapDH promoter"
                     /regulatory_class="promoter"
     CDS             10299..10946
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             10967..11512
                     /codon_start=1
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /protein_id="AYA22174.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            10967..11512
                     /gene="hpt"
                     /label="hpt"
     misc_feature    11697..12256
                     /label="second downstream homology recombination flank"
                     /note="second downstream homology recombination flank"
     terminator      12289..12477
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ttttatctga ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg
       61 ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa
      121 ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt
      181 ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg
      241 ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
      361 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
      421 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac
      481 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
      541 tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt
      601 ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt
      661 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata
      721 tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag
      781 cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacaaa aaaatccgct
      841 taagtccgcg ggacttcatg gcactttcta caccttgctt cataagactc ctttgctcca
      901 accattataa tgggatcgtc gtaattggca ggcttcccat ttataagcct ttgcgtgcga
      961 gtagcaggat tgccacactt catgcatata gcagtaagct tatcgacaaa ttcagctatg
     1021 gccatcaatt ctggagtcgg accaaatggt tcacctctaa agtccatgtc aagccctgca
     1081 catatgactc ttttcccgct atcggcaatc tcttttacga tgtcgactat ttcagaatca
     1141 aaaaattgaa cttcatctat agcaaataca tccgtatctt cttcagcata agccaatatg
     1201 tcagaagcct ttactatggc aatggcgtgc atgttgtcgc cgttatgaga tacgactttg
     1261 tctatggaat acctatcgtc tatagccggt ttgaaaacct gaactttttg cctggcaatc
     1321 ttagctctct ttatccttct tataagttcc tcactttttc cactgaacat gggaccagtt
     1381 acaacttcta tgtacccgtg gtctttaggc ccatacatct ttcgtcctcc ttaaaatttt
     1441 cgtttatttg ttttttattt agtgtgccta aaagcaccat cttatacatt cgtaccttat
     1501 atctcttatc ttgtatctct tacctcatat ctcttattat taactaccga agtatatttt
     1561 cataacggaa tcttatgact tatttttctg actgccgttt taccatacgg ttaatttata
     1621 cggaaacagc taatctaacc ttgctgaagg catgtttacc aaaatattat atctacttta
     1681 ccatattaat tcaagtggtc attttgatat tttacgccaa aatttttagc gttcctttac
     1741 ttgataattt atccaaaaac cttcattcgt caaaacaatt atgatataat taatttatga
     1801 tatgattaat taatatttac ggataactaa tcatttcaat gcaatagatc aacatgcaaa
     1861 tcaatatcca ccttactccg aacattttgc accaacgaat gcccagcact atatcccggc
     1921 aagcaatata acgtctataa ttttttatga taacgataaa attaaatttt atgaaagggg
     1981 taaaagcatg agaaaaatag ctgtattgtt aattactttg acgttcttag tcacgactcg
     2041 aatttaggag gcttacttgt ctgctttctt cattagaatc aatccttttt taaaagtcaa
     2101 tcccgtttgt tgaactactc tttaataaaa taatttttcc gttcccaatt ccacattgca
     2161 ataatagaaa atccatcttc atcggctttt tcgtcatcat ctgtatgaat caaatcgcct
     2221 tcttctgtgt catcaaggtt taatttttta tgtatttctt ttaacaaacc accataggag
     2281 attaaccttt tacggtgtaa accttcctcc aaatcagaca aacgtttcaa attcttttct
     2341 tcatcatcgg tcataaaatc cgtatccttt acaggatatt ttgcagtttc gtcaattgcc
     2401 gattgtatat ccgatttata tttatttttc ggtcgaatca tttgaacttt tacatttgga
     2461 tcatagtcta atttcattgc ctttttccaa aattgaatcc attgtttttg attcacgtag
     2521 ttttctgtat tcttaaaata agttggttcc acacatacca atacatgcat gtgctgatta
     2581 taagaattat ctttattatt tattgtcact tccgttgcac gcataaaacc aacaagattt
     2641 ttattaattt ttttatattg catcattcgg cgaaatcctt gagccatatc tgacaaactc
     2701 ttatttaatt cttcgccatc ataaacattt ttaactgtta atgtgagaaa caaccaacga
     2761 actgttggct tttgtttaat aacttcagca acaacctttt gtgactgaat gccatgtttc
     2821 attgctctcc tccagttgca cattggacaa agcctggatt tacaaaacca cactcgatac
     2881 aactttcttt cgcctgtttc acgattttgt ttatactcta atatttcagc acaatctttt
     2941 actctttcag cctttttaaa ttcaagaata tgcagaagtt caaagtaatc aacattagcg
     3001 attttctttt ctctccatgg tctcactttt ccactttttg tcttgtccac taaaaccctt
     3061 gatttttcat ctgaataaat gctactatta ggacacataa tattaaaaga aacccccatc
     3121 tatttagtta tttgtttggt cacttataac tttaacagat ggggtttttc tgtgcaacca
     3181 attttaaggg ttttcaatac tttaaaacac atacatacca acacttcaac gcacctttca
     3241 gcaactaaaa taaaaatgac gttatttcta tatgtatcaa gaatagaaag aactcgtttt
     3301 tcgctacgct caaaacgcaa aaaaagcact cattcgagtg ctttttctta tcgctccaaa
     3361 tcatgcgatt ttttcctctt tgcttttctt tgctcacgaa gttctcgatc acgctgcaaa
     3421 acatcttgaa gcgaaaaagt attcttcttt tcttccgatc gctcatgctg acgcacgaaa
     3481 agccctctag gcgcatagga acaactccta aatgcatgtg aggggttttc tcgtccatgt
     3541 gaacagtcgc atacgcaata ttttgtttcc catagcggcc gcgattatca aaaaggatct
     3601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagc
     3781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4681 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcga taggcgtggc
     4741 ttattgcccc cactgcgacg gaccgctgtt tgcagggaag gatgtggccg taattggcgg
     4801 aggcaattcc ggtatagaag ctgccattga tttggcgggc attgtaagac acgtaacggt
     4861 actggaattt ctgccgcagc tgaaagctga caaagtgctt caggaacgtt tgtacaggct
     4921 tcccaatgta actgtgctta caaatgtcca gacaaaagag tttaccggga aggaaaagct
     4981 cgacgggatt acttacattg agcgtgacac taatcaagaa aagcacattg aggttcaggg
     5041 agtgtttgtt cagattggcc ttgtcccaaa taccgaatgg ctggaaggaa ccattgaacg
     5101 caatgccatg ggtgaaatta ttgtcaatga aaaaaatgaa acctcaatgc ccggggtatt
     5161 tgcagcaggt gactgtacca acagccccta taaacagatt gttatagcca tgggttccgg
     5221 tgcgacggcc gcactcagcg ctttcgatta cctaatcagg aattaaggaa atattaaaat
     5281 ggaaatgttg aaaaaatgtt ttaagatggg tcattatgga taaaatatac tatggttttg
     5341 caataaatgc tttctattaa ttggactttg tggtaatatg gtagaaggat gcagtgttaa
     5401 ttttttaaca tataaaaata agctatatga agggagaatg gagaatgtcg aaggttatga
     5461 aaaccatgga tggcaataca gccgctgcac acgtagcata cgcctttaca gaagtagctg
     5521 ccatctatcc tataactcca tcatcaccta tggcagagca cgtcgatgaa tggagtgccc
     5581 acggcagaaa aaatctcttt gggcaagaag tgaaagtcat cgaaatgcag tcagaagcag
     5641 gtgctgctgg tgcagtacac ggttctcttg ctgctggtgc actaactaca acattcacag
     5701 catcacaagg attactattg atgattccta atatgtacaa gatcgctggt gaacttttgc
     5761 caggcgtatt ccacgttagc gctcgtgccc ttgcatcaca tgcgctatca atatttggtg
     5821 accatcaaga cgtaatggca tgcagacaaa caggatttgc gcttcttgca tcaggcagtg
     5881 ttcaggaagt catggactta ggctcagttg cacatttagc agctataaag ggtagagtgc
     5941 catttttgca tttctttgat ggttttagga catctcacga atatcagaag atagaagtaa
     6001 tggactatga agatttaaga aagcttttag atatggatgc agtaagagaa ttcaagaaga
     6061 gagcattaaa tccagagcat cctgtcacaa gaggcacagc tcaaaaccct gacatatact
     6121 tccaagaaag agaagcatcc aacaggtact acaatgctgt gccagaaata gtagaagaat
     6181 acatgaagga aataagcaag attacaggca gagagtacaa gctatttaac tactatggtg
     6241 ctcctgacgc tgaacgcatt gtaatcgcta tgggttcagt tacagagacg attgaggaga
     6301 caatcgacta cttgctgaaa aaaggcgaaa aagtaggtgt cgtaaaagta catctctaca
     6361 ggcctttctc atttaaacac ttcatggatg ctataccaaa gacagtaaag aagatagccg
     6421 tattagacag gacgaaagaa gcaggcgcat ttggcgaacc gctttatgaa gatgtaagag
     6481 cagctttcta cgacagtgaa atgaaaccta ttatcgtagg agggcgttac ggtttaggct
     6541 ctaaggacac tacaccagct caaatagtgg cagtatttga caacctaaag tcagatacac
     6601 cgaaaaacaa cttcacaatt ggcatagtgg atgatgtgac atacacatcg cttcctgttg
     6661 gtgaagaaat cgaaacaaca gcagaaggca ccataagctg caaattctgg ggatttggtt
     6721 cagacggcac agttggcgca aacaagagtg ctatacagat aatcggcgac aacactgata
     6781 tgtatgccca agcatacttc tcatacgact ctaagaagtc tggcggcgta acaatatccc
     6841 atttgcgctt tggcaaaaag ccaataaggt caacgtacct tatcaacaat gctgattttg
     6901 tagcatgcca taaacaggct tatgtgtaca actacgatgt gcttgcaggc ctcaaaaaag
     6961 gcggcacatt cttgttaaac tgcacatgga aaccagaaga actcgatgaa aagctacctg
     7021 cttctatgaa gaggtacatc gctaagaaca acatcaattt ctacatcata aatgcagttg
     7081 acatagcaaa agaattaggt cttggcgcaa gaataaacat gataatgcaa tcagcattct
     7141 ttaagcttgc gaacatcatt cctattgatg aagcagttaa acacttaaaa gatgctattg
     7201 tgaagtctta cggtcataaa ggcgaaaaga tagtcaacat gaactacgca gccgtcgatc
     7261 gtggaataga tgcacttgtt aaggtagatg ttcctgcatc gtgggcaaat gctgaagacg
     7321 aagctaaagt agaaagaaat gttcctgatt tcataaagaa tatagcagat gttatgaata
     7381 gacaagaagg agacaaattg cctgtaagcg cctttgtagg catggaagac ggtacattcc
     7441 caatgggcac tgcagcatac gagaagcgcg gtatcgccgt agatgtgcct gaatggcaga
     7501 tagataactg catacagtgc aatcagtgtg cgtatgtatg tccacatgca gcaatcaggc
     7561 cattcctctt aaatgaagaa gaagtcaaaa acgctcctga aggatttact tccaaaaaag
     7621 ctatcggtaa gggcttagaa ggattgaact tcaggattca agtgagcgtg cttgattgta
     7681 caggctgtgg cgtatgcgca aacacatgtc cttcaaaaga aaagtctctc ataatgaagc
     7741 ctttagagac acagttagat caagcaaaga attgggaata cgctatgtca ttatcataca
     7801 aggagaatcc attaggaaca gatacggtaa agggcagcca atttgagaag cctttgcttg
     7861 aattctctgg tgcttgcgca ggatgcggcg aaacaccata cgcaaggctt gtaacacaat
     7921 tatttggcga tagaatgctt atcgcaaatg ctacaggttg ctcatccatc tggggtggca
     7981 gcgcaccatc tacaccgtac acagtaaaca aagatggtca tggtccagct tgggctaact
     8041 cactgtttga agacaacgct gagtttggct tcggtatggc attagctgta aaacaacaga
     8101 gagaaaaatt ggctgatata gtaaaagaag cattagaatt agatcttacg caagatttaa
     8161 agaatgcgtt aaagttatgg cttgacaatt tcaatagctc tgagataaca aagaagacag
     8221 ccaatatcat agtttcattg atacaggact acaagacaga tgactcaaaa gtcaaagaac
     8281 ttttaaatga aatacttgat aggaaagaat atcttgtcaa gaagtcacag tggatatttg
     8341 gcggtgacgg ttgggcatac gatataggct ttggtggtct tgaccatgtg ctggcatccg
     8401 gtgaagacgt aaatgttctt gtatttgata cagaagtgta ttcaaacaca ggtggtcaat
     8461 catcaaaggc gacaccagta ggcgctatag cacaatttgc tgcagcaggt aaaggaatcg
     8521 gcaagaagga tttaggacgc attgcaatga gctatggata tgtttatgtt gcgcagatag
     8581 ctatgggcgc taaccaagca cagacaataa aagccttgaa agaggcagaa agctatcctg
     8641 gcccatcgct tataatagca tatgctccat gtatcaacca tggcatcaag cttggaatgg
     8701 gttgcagcca gatagaagaa aagaaagcag ttgaagctgg atactggcac ctatacaggt
     8761 acaatccaat gcttaaagct gagggcaaga atccattcat ccttgactca aaggctccaa
     8821 cagcttcata caaagaattc atcatgggag aagttcgcta ttcttcactg gcaaagacat
     8881 tcccagaaag agcagaagct ttgtttgaaa aagctgaaga acttgcaaaa gaaaaatacg
     8941 aaacatacaa gaagcttgct gaacaaaatt agtaaaggag gcgatattaa tggcacatat
     9001 tattacagac gaatgcataa gctgtggtgc ttgcgctgca gaatgccctg tagatgctat
     9061 tcacgaaggt acaggaaaat acgaagtgga tgctgataca tgtatagatt gtggtgcatg
     9121 tgaaccagta tgcccaacag gagctattaa ggctgaataa gaaattaaca gaggacggtt
     9181 ctttgtttga tatcaaacag ggaaccgtcc aaactattgg tgaaagtata atagatagat
     9241 ttttaccttg aaaaatcata gtatttgtct gtacgatacc aattccaaaa attttgtcct
     9301 tgtgtcatga gaagctggtc taaaaatact tcaaaattat gtccaagaca tttaaactca
     9361 tcgcgcaaaa tatttggtcc gacatacaaa taattgttgg tattggcttc tttgctgttg
     9421 attactatat aaatttccgt tttggattct tttgattgcg gagattatat cttgcatgct
     9481 caagtaaatc aactcccaca taataatttt tttattattt actcctgctt taggcaaaag
     9541 aacaaaattt aacatcgaca aattaacata gttgtttatt tgtatcaagc agaaaaaaat
     9601 atgtatatga tgccggccga ccgacacaaa aatgtgagcg ttcacgaaac aataaatata
     9661 taaataaggg tattaattct gcaaaaagaa aagtgtttgc tacatgaggt ccattaattt
     9721 atcgtgggaa taggcatgga aaagattttt ttgcccgagg atgcattgat aaagtatttc
     9781 ttttccgaaa gagaggaaaa gattctaaag agttttggaa atactgatga atattgtgtg
     9841 cagagtacaa ttctatggac aagaaaagag gctttgtcaa aactttttcg tctgggaatg
     9901 aggatggatt ttaaaaagct ggatactttg gagggcgagg tggtttttca ggaaacaaac
     9961 agggcgcgtc tgttttcttt tatatgcaat aattactgta tctctctggc attgccaggt
    10021 tttaataaag attaaaatta ttgactagaa ataaaaaaat tgtccataat attaatggac
    10081 aaaaaaacaa agaattacat caaaggaaga taaaaatact ttgttaaaaa attaattatt
    10141 ttttatctaa actattgaaa atgaaaataa aataatataa aatgaatcat agtgcaagag
    10201 atacttgcca gaggatgaat attttactgc attcatgctt tatggcagct aatagaggca
    10261 ttaaattaaa ttttaattta caataggagg cgatattaat gaactttaat aaaattgatt
    10321 tagacaattg gaagagaaaa gagatattta atcattattt gaaccaacaa acgactttta
    10381 gtataaccac agaaattgat attagtgttt tataccgaaa cataaaacaa gaaggatata
    10441 aattttaccc tgcatttatt ttcttagtga caagggtgat aaactcaaat acagctttta
    10501 gaactggtta caatagcgac ggagagttag gttattggga taagttagag ccactttata
    10561 caatttttga tggtgtatct aaaacattct ctggtatttg gactcctgta aagaatgact
    10621 tcaaagagtt ttatgattta tacctttctg atgtagagaa atataatggt tcggggaaat
    10681 tgtttcccaa aacacctata cctgaaaatg ctttttctct ttctattatt ccatggactt
    10741 catttactgg gtttaactta aatatcaata ataatagtaa ttaccttcta cccattatta
    10801 cagcaggaaa attcattaat aaaggtaatt caatatattt accgctatct ttacaggtac
    10861 atcattctgt ttgtgatggt tatcatgcag gattgtttat gaactctatt caggaattgt
    10921 cagataggcc taatgactgg cttttataat aaaggaggtc gacgtcatgg aaaatttatc
    10981 aaaagacatc gatgaaattt tgatcacaga agaagaactt aaggaaaaga taaaagagct
    11041 tgggaggcaa atcacaaaag actacaaagg gaaaaatttg atgttggtag gagttttaaa
    11101 aggtgcttta atgtttatgg ctgatttgtc aagacacata gatttgcctt tatcacttga
    11161 ttttatggct gtttccagct atggaagctc aactcattca tcaggaatag taaagataat
    11221 caaagatctt gatataagca tagaaggcaa agatgttctg attgtggaag acataattga
    11281 cagcggtttg actttgtctt acttaaggga aactttactt ggaaggaagc caaaaagcct
    11341 gaaaatatgc acaatattag acaaaccgga gagaagagaa gcatctgtaa aagtcgatta
    11401 tgtaggattt aagatacctg ataagtttgt cgtgggttat ggattggact ttgatgaaaa
    11461 gtacaggaac cttcctttta taggcgtttt gaaacctgaa atgtacagct aagcccgggc
    11521 ctcgagaaaa caaaaggctc agtcggaaga ctgggccttt tgttttggta ccgaattcgg
    11581 cgcgcctcag cgtttaaacc cgctgatcct agagggccgc atcatgtaat tagttatgtc
    11641 acgcttacat tcacgccctc cccatgcttt aatacatacc tggcccagta gttcaggaaa
    11701 ttaacagagg acggttcttt gtttgatatc aaacagggaa ccgtccaaac tattggtgaa
    11761 agtataatag atagattttt accttgaaaa atcatagtat ttgtctgtac gataccaatt
    11821 ccaaaaattt tgtccttgtg tcatgagaag ctggtctaaa aatacttcaa aattatgtcc
    11881 aagacattta aactcatcgc gcaaaatatt tggtccgaca tacaaataat tgttggtatt
    11941 ggcttctttg ctgttgatta ctatataaat ttccgttttg gattcttttg attgcggaga
    12001 ttatatcttg catgctcaag taaatcaact cccacataat aattttttta ttatttactc
    12061 ctgctttagg caaaagaaca aaatttaaca tcgacaaatt aacatagttg tttatttgta
    12121 tcaagcagaa aaaaatatgt atatgatgcc ggccgaccga cacaaaaatg tgagcgttca
    12181 cgaaacaata aatatataaa taagggtatt aattctgcaa aaagaaaagt gtttgctaca
    12241 tgaggtccat taatttctgc taatagtagt gaaaaaatca gggataatgc cgctctaacc
    12301 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
    12361 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
    12421 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
    12481 ttaatttgca agctgcggc
//
