LOCUS       V003245                14612 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003245
VERSION     V003245
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Lanahan AA, Murphy SJL., Maloney MI,
            Zheng T, Papanek B, Guss AM, Lynd LR.
  TITLE     The ethanol pathway from Thermoanaerobacterium saccharolyticum
            improves ethanol production in Clostridium thermocellum
  JOURNAL   Metab. Eng. 42, 175-184 (2017)
   PUBMED   28663138
REFERENCE   2  (bases 1 to 14612)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Lanahan AA, Murphy SJL., Maloney MI,
            Zheng T, Papanek BA, Guss AM, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2017) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 14612)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14612)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            42, 175-184 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-MAR-2017) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..579)
                     /codon_start=1
                     /gene="tdk"
                     /product="Tdk"
                     /label="tdk"
                     /note="thymidine kinase"
                     /protein_id="ASK05943.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(1..579)
                     /gene="tdk"
                     /label="tdk"
     regulatory      complement(580..1200)
                     /label="C therm cbp promoter"
                     /note="C therm cbp promoter"
                     /regulatory_class="promoter"
     CDS             complement(1279..2280)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(2281..2735)
                     /direction=LEFT
                     /label="C thermocellum replication origin"
                     /note="C thermocellum replication origin"
     CDS             complement(2841..3698)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3699..3803)
                     /label="AmpR promoter"
     misc_feature    3893..4392
                     /label="upstream homology region"
                     /note="upstream homology region"
     regulatory      4396..4970
                     /label="C thermocellum gapDH promoter"
                     /note="C thermocellum gapDH promoter"
                     /regulatory_class="promoter"
     CDS             4971..5618
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             5639..6184
                     /codon_start=1
                     /gene="hpt"
                     /product="Hpt"
                     /label="hpt"
                     /note="hypoxanthine phosphoribosyltransferase"
                     /protein_id="ASK05947.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            5639..6184
                     /gene="hpt"
                     /label="hpt"
     misc_feature    6369..6868
                     /label="upstream homology region"
                     /note="upstream homology region"
     CDS             6869..8068
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="NADPH-dependent alcohol dehydrogenase"
                     /protein_id="ASK05948.1"
                     /translation="MWETKVNPSKIFELRCKNTTYFGVGSIHKIKDILENLKINGINNV
                     IFITGKGSYKTSGAWDVVRPVLEELDLKYSLYDKVGPNPTVDMIDEAAKIGRESGAKAV
                     IGIGGGSPIDTAKSVAVLLKYTDKNARELYKQKFIPDDAVPIIAINLTHGTGTEVDRFA
                     VATIPEKNYKPAIAYDCLYPMFAIDDPSLMTKLDKKQTIAVTVDALNHITEAATTLVAS
                     PYSILTAKETVRLIVRYLPAAVNDPLNIVARYYLLYASALAGISFDNGLLHLTHALEHP
                     LSAVKPEIAHGLGLGAILPAVIKAIYPATAEVLADVYSPIVPGLKGLPVEAEYVAEKVQ
                     EWLFSVGCIQKLSDFGFTKDDIPNLVKLAKTTPSLDGLLSIAPVEATESVIEKIYLKSL
                     "
     gene            6869..8068
                     /gene="adhA"
                     /label="adhA"
     CDS             8086..8940
                     /codon_start=1
                     /gene="nfnA"
                     /product="NfnA"
                     /label="nfnA"
                     /note="NADH-dependent reduced ferredoxin:NADP+
                     oxidoreductase"
                     /protein_id="ASK05949.1"
                     /translation="MNEILEKKQLNPTVKMMVINAPLMAKKAKPGQFVIVRVDEKGERI
                     PLTIADYDRNKGTITIIFQEVGMSTKKLGTLNVGDRLHDFVGPLGKPVEFSKDTKRVLA
                     IGGGVGVAPLYPKVKMLNEMKVPVDSIIGGRSAEYVILEDEMKKVSENLYITTDDGTKG
                     RKGFVTDVLKELIEKDNKYDEVIAIGPLIMMKMVCNITKEYNIPTMVSMNPIMIDGTGM
                     CGGCRVTVGGETKFACVDGPAFDGLKVDFDEAMRRQNMYKDMERKVLENYEHECKLGGI
                     LNG"
     gene            8086..8940
                     /gene="nfnA"
                     /label="nfnA"
     CDS             8933..10324
                     /codon_start=1
                     /gene="nfnB"
                     /product="NfnB"
                     /label="nfnB"
                     /note="NADH-dependent reduced ferredoxin:NADP+
                     oxidoreductase"
                     /protein_id="ASK05950.1"
                     /translation="MANMSLKKVPMPEQEPDQRNKNFKEVALGYEENMAVEEAERCIQC
                     KNQPCVEGCPVHVKIPEFIKLIANRDFEGAYQKIKETNNLPAICGRVCPQESQCESVCT
                     RGKKGEPVAIGRLERFTADWHMKNNEDKIEKPETNGRKVAVIGSGPAGLSCAGDLAKMG
                     YDTTIFEAFHTPGGVLMYGIPEFRLPKEIVQKEIDSLKKLGVKIETNMVIGKILTIDDL
                     FDMGYEAVFIGTGAGLPKFMNIPGENLNGVYSANEFLTRINLMKAYDFPNSPTPVKVGK
                     KVAVVGGGNVAMDAARSAKRMGAEEVYIVYRRSEEEMPARLEEIHHAKEEGIIFKLLTN
                     PVRIIGDESGSVKGIECVNMVLGDVDESGRRRPVEEKGSEHVIDVDTVIIAIGQSPNPL
                     ITSTTEGLEKQRWGGIIVNEETLETSRRGVFAGGDAVTGAATVILAMGAGKKAAASIHK
                     YLSEK"
     gene            8933..10324
                     /gene="nfnB"
                     /label="nfnB"
     CDS             10342..12921
                     /codon_start=1
                     /gene="adhE"
                     /product="AdhE"
                     /label="adhE"
                     /note="bifunctional alcohol and aldehyde dehydrogenase;
                     bifunctional alcohol and aldehyde dehydrognase"
                     /protein_id="ASK05951.1"
                     /translation="MATTKTELDVQKQIDLLVSRAQEAQKKFMSYTQEQIDAIVKAMAL
                     AGVDKHVELAKMAYEETKMGVYEDKITKNLFATEYVYHDIKNEKTVGIINENIEENYME
                     VAEPIGVIAGVTPVTNPTSTTMFKCLISIKTRNPIIFSFHPKAIKCSIAAAKVMYEAAL
                     KAGAPEGCIGWIETPSIEATQLLMTHPGVSLILATGGAGMVKAAYSSGKPALGVGPGNV
                     PCYIEKSANIKRAVSDLILSKTFDNGVICASEQAVIIDEEIADEVKKLMKEYGCYFLNK
                     DEIKKLEKFAIDEQSCAMSPAVVGQPAAKIAEMAGFKVPEGTKILVAEYEGVGPKYPLS
                     REKLSPILACYTVKDYNEGIKKCEEMTEFGGLGHSAVIHSENQNVINEFARRVRTGRLI
                     VNSPSSQGAIGDIYNTNTPSLTLGCGSMGRNSTTDNVSVKNLLNIKRVVIRKDRMKWFK
                     IPPKIYFESGSLQYLCKVKRKKAFIVTDPFMVKLGFVDKVTYQLDKANIEYEIFSEVEP
                     DPSVDTVMNGVKIMNSYNPDLIIAVGDGSAIDAAKGMWLFYEYPDTEFETLRLKFADIR
                     KRAFKFPELGKKALFIAIPTTSGTGSEVTAFAVITDKKRNIKYPLADYELTPDIAIIDP
                     DLTKTVPPSVTADTGMDVLTHAIEAYVSVMASDYTDALAEKAIKIVFEYLPRAYKNGND
                     EEAREKMHNASCMAGMAFTNAFLGINHSMAHILGGKFHIPHGRANAILLPYVIRYNAEK
                     PTKFVAFPQYEYPKAAERYAEIAKFLGLPASTVEEGVESLIEAIKNLMKELNIPLTLKD
                     AGINKEQFEKEIEEMSDIAFNDQCTGTNPRMPLTKEIAEIYRKAYGA"
     gene            10342..12921
                     /gene="adhE"
                     /label="adhE"
     misc_feature    12939..13457
                     /label="downstream homology region"
                     /note="downstream homology region"
     terminator      13490..13678
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(13938..14526)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ttaagtccgc gggacttcat ggcactttct acaccttgct tcataagact cctttgctcc
       61 aaccattata atgggatcgt cgtaattggc aggcttccca tttataagcc tttgcgtgcg
      121 agtagcagga ttgccacact tcatgcatat agcagtaagc ttatcgacaa attcagctat
      181 ggccatcaat tctggagtcg gaccaaatgg ttcacctcta aagtccatgt caagccctgc
      241 acatatgact cttttcccgc tatcggcaat ctcttttacg atgtcgacta tttcagaatc
      301 aaaaaattga acttcatcta tagcaaatac atccgtatct tcttcagcat aagccaatat
      361 gtcagaagcc tttactatgg caatggcgtg catgttgtcg ccgttatgag atacgacttt
      421 gtctatggaa tacctatcgt ctatagccgg tttgaaaacc tgaacttttt gcctggcaat
      481 cttagctctc tttatccttc ttataagttc ctcacttttt ccactgaaca tgggaccagt
      541 tacaacttct atgtacccgt ggtctttagg cccatacatc tttcgtcctc cttaaaattt
      601 tcgtttattt gttttttatt tagtgtgcct aaaagcacca tcttatacat tcgtacctta
      661 tatctcttat cttgtatctc ttacctcata tctcttatta ttaactaccg aagtatattt
      721 tcataacgga atcttatgac ttatttttct gactgccgtt ttaccatacg gttaatttat
      781 acggaaacag ctaatctaac cttgctgaag gcatgtttac caaaatatta tatctacttt
      841 accatattaa ttcaagtggt cattttgata ttttacgcca aaatttttag cgttccttta
      901 cttgataatt tatccaaaaa ccttcattcg tcaaaacaat tatgatataa ttaatttatg
      961 atatgattaa ttaatattta cggataacta atcatttcaa tgcaatagat caacatgcaa
     1021 atcaatatcc accttactcc gaacattttg caccaacgaa tgcccagcac tatatcccgg
     1081 caagcaatat aacgtctata attttttatg ataacgataa aattaaattt tatgaaaggg
     1141 gtaaaagcat gagaaaaata gctgtattgt taattacttt gacgttctta gtcacgactc
     1201 gaatttagga ggcttacttg tctgctttct tcattagaat caatcctttt ttaaaagtca
     1261 atcccgtttg ttgaactact ctttaataaa ataatttttc cgttcccaat tccacattgc
     1321 aataatagaa aatccatctt catcggcttt ttcgtcatca tctgtatgaa tcaaatcgcc
     1381 ttcttctgtg tcatcaaggt ttaatttttt atgtatttct tttaacaaac caccatagga
     1441 gattaacctt ttacggtgta aaccttcctc caaatcagac aaacgtttca aattcttttc
     1501 ttcatcatcg gtcataaaat ccgtatcctt tacaggatat tttgcagttt cgtcaattgc
     1561 cgattgtata tccgatttat atttattttt cggtcgaatc atttgaactt ttacatttgg
     1621 atcatagtct aatttcattg cctttttcca aaattgaatc cattgttttt gattcacgta
     1681 gttttctgta ttcttaaaat aagttggttc cacacatacc aatacatgca tgtgctgatt
     1741 ataagaatta tctttattat ttattgtcac ttccgttgca cgcataaaac caacaagatt
     1801 tttattaatt tttttatatt gcatcattcg gcgaaatcct tgagccatat ctgacaaact
     1861 cttatttaat tcttcgccat cataaacatt tttaactgtt aatgtgagaa acaaccaacg
     1921 aactgttggc ttttgtttaa taacttcagc aacaaccttt tgtgactgaa tgccatgttt
     1981 cattgctctc ctccagttgc acattggaca aagcctggat ttacaaaacc acactcgata
     2041 caactttctt tcgcctgttt cacgattttg tttatactct aatatttcag cacaatcttt
     2101 tactctttca gcctttttaa attcaagaat atgcagaagt tcaaagtaat caacattagc
     2161 gattttcttt tctctccatg gtctcacttt tccacttttt gtcttgtcca ctaaaaccct
     2221 tgatttttca tctgaataaa tgctactatt aggacacata atattaaaag aaacccccat
     2281 ctatttagtt atttgtttgg tcacttataa ctttaacaga tggggttttt ctgtgcaacc
     2341 aattttaagg gttttcaata ctttaaaaca catacatacc aacacttcaa cgcacctttc
     2401 agcaactaaa ataaaaatga cgttatttct atatgtatca agaatagaaa gaactcgttt
     2461 ttcgctacgc tcaaaacgca aaaaaagcac tcattcgagt gctttttctt atcgctccaa
     2521 atcatgcgat tttttcctct ttgcttttct ttgctcacga agttctcgat cacgctgcaa
     2581 aacatcttga agcgaaaaag tattcttctt ttcttccgat cgctcatgct gacgcacgaa
     2641 aagccctcta ggcgcatagg aacaactcct aaatgcatgt gaggggtttt ctcgtccatg
     2701 tgaacagtcg catacgcaat attttgtttc ccatagcggc cgcgattatc aaaaaggatc
     2761 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2821 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2881 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2941 cgcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3001 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3061 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3121 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3181 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3241 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3301 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3361 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3421 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3481 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3541 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3601 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3661 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3721 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3781 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3841 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcg attctggatg
     3901 caataaacaa cggagccgtt aaggctataa aagaaagcta tttgaaactt gatgaagaac
     3961 ttggcaaatt taacagaaac ccccacgtgg acagggaaga tggttaatgt gattttgagc
     4021 aaggggaggg aaagggtatt gtatgcctaa gccatcaatc ggaactgctc aaattccagt
     4081 gccggagaaa gaggaaaagc cggtaatcca ggagcttacc gtacttgaag aaggctcaaa
     4141 ggaatcaata tataatctga ttttttctga agaagaaaaa taagataaac aaaggacggt
     4201 tcagggcttc tgctcatcct actctgcatt gtaaaaaggt aggatgaatt tttattttta
     4261 atcttattga aaaaaatttt tgaaaatcgg ttttattaaa aaaaagtggg tatatttata
     4321 atagtcaatt gattggttaa aaaaatttaa ataagcaaac agaataataa caaaagtaag
     4381 gaggaatttg ttatcgtggg aataggcatg gaaaagattt ttttgcccga ggatgcattg
     4441 ataaagtatt tcttttccga aagagaggaa aagattctaa agagttttgg aaatactgat
     4501 gaatattgtg tgcagagtac aattctatgg acaagaaaag aggctttgtc aaaacttttt
     4561 cgtctgggaa tgaggatgga ttttaaaaag ctggatactt tggagggcga ggtggttttt
     4621 caggaaacaa acagggcgcg tctgttttct tttatatgca ataattactg tatctctctg
     4681 gcattgccag gttttaataa agattaaaat tattgactag aaataaaaaa attgtccata
     4741 atattaatgg acaaaaaaac aaagaattac atcaaaggaa gataaaaata ctttgttaaa
     4801 aaattaatta ttttttatct aaactattga aaatgaaaat aaaataatat aaaatgaatc
     4861 atagtgcaag agatacttgc cagaggatga atattttact gcattcatgc tttatggcag
     4921 ctaatagagg cattaaatta aattttaatt tacaatagga ggcgatatta atgaacttta
     4981 ataaaattga tttagacaat tggaagagaa aagagatatt taatcattat ttgaaccaac
     5041 aaacgacttt tagtataacc acagaaattg atattagtgt tttataccga aacataaaac
     5101 aagaaggata taaattttac cctgcattta ttttcttagt gacaagggtg ataaactcaa
     5161 atacagcttt tagaactggt tacaatagcg acggagagtt aggttattgg gataagttag
     5221 agccacttta tacaattttt gatggtgtat ctaaaacatt ctctggtatt tggactcctg
     5281 taaagaatga cttcaaagag ttttatgatt tatacctttc tgatgtagag aaatataatg
     5341 gttcggggaa attgtttccc aaaacaccta tacctgaaaa tgctttttct ctttctatta
     5401 ttccatggac ttcatttact gggtttaact taaatatcaa taataatagt aattaccttc
     5461 tacccattat tacagcagga aaattcatta ataaaggtaa ttcaatatat ttaccgctat
     5521 ctttacaggt acatcattct gtttgtgatg gttatcatgc aggattgttt atgaactcta
     5581 ttcaggaatt gtcagatagg cctaatgact ggcttttata ataaaggagg tcgacgtcat
     5641 ggaaaattta tcaaaagaca tcgatgaaat tttgatcaca gaagaagaac ttaaggaaaa
     5701 gataaaagag cttgggaggc aaatcacaaa agactacaaa gggaaaaatt tgatgttggt
     5761 aggagtttta aaaggtgctt taatgtttat ggctgatttg tcaagacaca tagatttgcc
     5821 tttatcactt gattttatgg ctgtttccag ctatggaagc tcaactcatt catcaggaat
     5881 agtaaagata atcaaagatc ttgatataag catagaaggc aaagatgttc tgattgtgga
     5941 agacataatt gacagcggtt tgactttgtc ttacttaagg gaaactttac ttggaaggaa
     6001 gccaaaaagc ctgaaaatat gcacaatatt agacaaaccg gagagaagag aagcatctgt
     6061 aaaagtcgat tatgtaggat ttaagatacc tgataagttt gtcgtgggtt atggattgga
     6121 ctttgatgaa aagtacagga accttccttt tataggcgtt ttgaaacctg aaatgtacag
     6181 ctaagcccgg gcctcgagaa aacaaaaggc tcagtcggaa gactgggcct tttgttttgg
     6241 taccgaattc ggcgcgcctc agcgtttaaa cccgctgatc ctagagggcc gcatcatgta
     6301 attagttatg tcacgcttac attcacgccc tccccatgct ttaatacata cctggcccag
     6361 tagttcagtc tggatgcaat aaacaacgga gccgttaagg ctataaaaga aagctatttg
     6421 aaacttgatg aagaacttgg caaatttaac agaaaccccc acgtggacag ggaagatggt
     6481 taatgtgatt ttgagcaagg ggagggaaag ggtattgtat gcctaagcca tcaatcggaa
     6541 ctgctcaaat tccagtgccg gagaaagagg aaaagccggt aatccaggag cttaccgtac
     6601 ttgaagaagg ctcaaaggaa tcaatatata atctgatttt ttctgaagaa gaaaaataag
     6661 ataaacaaag gacggttcag ggcttctgct catcctactc tgcattgtaa aaaggtagga
     6721 tgaattttta tttttaatct tattgaaaaa aatttttgaa aatcggtttt attaaaaaaa
     6781 agtgggtata tttataatag tcaattgatt ggttaaaaaa atttaaataa gcaaacagaa
     6841 taataacaaa agtaaggagg aatttgttat gtgggaaaca aaagtaaatc ctagcaaaat
     6901 ttttgaactt cgctgtaaaa atacgactta ttttggtgtt ggcagtatac ataagataaa
     6961 ggatatatta gaaaatctaa aaattaatgg cattaataat gttattttta taacaggcaa
     7021 aggttcgtat aagactagcg gagcttggga tgttgtaagg cctgtattgg aagagcttga
     7081 tttaaaatat tcactttacg ataaagttgg tccaaatcct accgttgata tgatagacga
     7141 agcggcaaaa attggacgag aatctggagc gaaagccgta attggaattg gcggtggtag
     7201 ccctatagat actgcaaaaa gtgtagctgt gctattaaag tatactgata aaaatgcacg
     7261 agagctttat aagcaaaaat ttattcctga tgatgctgtt cctataatag ctataaatct
     7321 tacacatggt acaggaacag aggtagatag atttgcagtg gctactatac ctgagaaaaa
     7381 ttataaacca gctatagctt atgattgctt atatcctatg tttgctatag atgacccttc
     7441 gcttatgaca aagcttgata aaaaacagac tatagccgtt actgttgatg ctttaaatca
     7501 cattacagaa gctgctacga cacttgttgc atctccatat agtattctta cggctaagga
     7561 aacagtaaga ttgattgttc gatatcttcc cgctgctgta aatgatcctt taaatatagt
     7621 ggcaagatat taccttctgt atgcttctgc attagctggc atatcttttg acaatggtct
     7681 tttgcatctt actcatgctt tagagcatcc tttaagtgct gtaaaacctg aaatagctca
     7741 tggtttaggg cttggtgcaa tattgccagc agttataaaa gctatttatc cagctacagc
     7801 agaagtattg gctgatgtat atagtcctat agttcctggt ttaaaaggac tgcctgttga
     7861 ggcggagtat gtagcagaaa aagttcaaga atggcttttt agtgtggggt gcattcaaaa
     7921 gttatctgat tttgggttta cgaaagatga tattcctaat cttgtaaaac ttgctaaaac
     7981 aactccttcc cttgatgggt tgttatctat tgcacctgtt gaagctactg aatctgtcat
     8041 tgaaaagatt tatttaaaat ctctttaata aaggaggtta taaaaatgaa cgagatttta
     8101 gagaagaagc aattgaatcc cactgtcaaa atgatggtta taaacgctcc actaatggcg
     8161 aaaaaggcga aacctggaca gtttgtcata gtaagagtag atgaaaaggg tgaacgcatt
     8221 cctttgacaa tagcggatta cgacagaaac aaaggcacga ttactataat atttcaagaa
     8281 gtcggcatga gcaccaagaa gttaggtacg ttaaacgtag gcgatagatt gcatgacttc
     8341 gtaggacctt taggaaaacc tgttgaattt tcaaaagata cgaagagagt tttagcaatc
     8401 ggcggaggtg ttggagttgc gccgctttat ccaaaagtaa aaatgctaaa tgagatgaaa
     8461 gtaccagtgg atagtataat aggcggcaga agtgcagagt acgtcatatt agaagatgaa
     8521 atgaaaaaag tcagtgaaaa cctttatata acgacggatg acggcacaaa aggccgtaaa
     8581 ggctttgtaa cagatgtatt aaaagaattg atagaaaaag acaacaaata cgatgaagta
     8641 attgcaattg ggccactgat aatgatgaag atggtatgca atataacaaa agaatacaac
     8701 ataccaacta tggtcagcat gaacccgata atgatagacg gaactggaat gtgcggagga
     8761 tgcagagtca cagttggagg agagacaaaa tttgcatgtg tcgatggtcc agcatttgac
     8821 gggcttaaag tagactttga tgaagcaatg agaaggcaga atatgtataa agatatggag
     8881 aggaaagtat tagaaaatta tgagcacgaa tgcaagttag ggggcatctt aaatggctaa
     8941 catgtcgtta aaaaaagtac caatgcctga acaggaacca gatcagcgga acaaaaattt
     9001 caaagaagtt gcattagggt atgaagaaaa catggctgtt gaagaagccg aaagatgtat
     9061 tcaatgtaag aatcagccat gtgttgaagg ttgccctgtt cacgtaaaaa tacctgagtt
     9121 catcaagctg atagcaaaca gggactttga aggagcatat caaaagataa aagaaacgaa
     9181 taatctacct gcaatatgtg gaagagtatg tccacaggaa agccagtgtg aatcagtatg
     9241 cacaagaggc aaaaaaggag aacctgtagc tataggcaga ctggagagat ttacagcaga
     9301 ttggcatatg aaaaataatg aagataaaat agaaaaacca gagacgaatg gcagaaaagt
     9361 tgctgttata ggatctgggc ctgcaggact atcgtgtgca ggtgatcttg caaaaatggg
     9421 atatgacaca accatctttg aagcatttca tacaccaggc ggtgttttaa tgtacggtat
     9481 cccagagttt agacttccaa aagaaatagt gcaaaaagaa attgattcgt tgaaaaaatt
     9541 gggagtaaaa atagaaacca acatggtcat aggcaagata cttacaatcg atgatttatt
     9601 cgacatgggg tatgaagcag tgttcatagg gacaggcgca ggattgccta aatttatgaa
     9661 tatacctgga gaaaacttaa atggagttta ctcagcaaat gaatttttga cgaggattaa
     9721 tttgatgaaa gcttatgatt tccctaacag tcccactcct gtaaaagtag gcaagaaagt
     9781 tgctgttgtt ggcggtggaa acgtggcgat ggatgcagca agatccgcaa agcgcatggg
     9841 agcagaagaa gtgtacatcg tatatagaag gtcagaggaa gaaatgcctg caaggcttga
     9901 ggaaatacac cacgcaaaag aagaaggaat catatttaag ttattgacaa accctgtaag
     9961 aatcattggt gatgaaagtg gcagtgtaaa aggaatcgaa tgtgttaaca tggttttagg
    10021 cgatgtagac gaatcaggta gaagaagacc tgtagaagaa aaaggctcag agcatgtaat
    10081 agatgtagat acagttatta tagcaatagg acaaagtcca aatccgctta taacatccac
    10141 aacagaaggt ttagaaaagc aaagatgggg tggaataata gtcaacgaag aaacattgga
    10201 gacaagccgc agaggagttt ttgcgggcgg tgatgctgtg acaggtgcag ctacagtaat
    10261 acttgcaatg ggagctggta aaaaagctgc agcttctata cacaaatatt taagcgaaaa
    10321 gtgataaagg aggtcgacgt catggcaacg acaaaaacgg aattagacgt tcagaagcag
    10381 atagatctac ttgtgtcaag agcacaagag gctcagaaaa aattcatgtc ttacacgcaa
    10441 gagcaaatcg acgcaatagt taaggcaatg gctttagcag gcgttgacaa acacgtagag
    10501 ctggcaaaga tggcgtacga agagacaaaa atgggtgtat acgaagataa gataacaaaa
    10561 aatctcttcg caacagagta cgtgtaccac gacataaaaa atgaaaagac tgtaggaatc
    10621 ataaacgaga acatagaaga aaactacatg gaagtggcag aaccgatagg cgtaattgcc
    10681 ggtgtcacac ctgtcacaaa cccaacatct accacgatgt ttaaatgctt aatatccata
    10741 aagacgcgaa atcctataat attcagcttc catccaaagg caataaagtg cagcatcgca
    10801 gcagccaaag tgatgtatga agctgcacta aaggcaggcg cacctgaagg atgcatagga
    10861 tggatagaaa cgccatcaat tgaggccaca cagcttctca tgacacatcc aggcgtatcg
    10921 ctgatccttg caacgggcgg tgcaggaatg gtaaaagcgg catacagctc aggaaaaccg
    10981 gcattaggcg taggtcctgg caatgtgcca tgctacatcg aaaaatcagc aaacataaag
    11041 agggctgtat cggatctcat actaagcaag acatttgaca atggagtaat atgcgcatca
    11101 gagcaggccg taataataga cgaggaaata gcagatgaag tcaaaaagct tatgaaagaa
    11161 tacggctgct acttcttaaa caaagatgaa ataaagaagc ttgagaaatt tgcaattgat
    11221 gagcaaagct gcgccatgag ccctgcagtg gtaggtcagc cagcggcgaa gattgctgaa
    11281 atggcaggct tcaaagtccc cgaaggcaca aagatattag tggcagagta cgaaggagta
    11341 ggtccaaaat atcctctatc aagggagaaa ctaagcccga ttcttgcttg ctacaccgtc
    11401 aaagactaca atgaaggaat caaaaagtgc gaggaaatga ctgaattcgg aggtttaggc
    11461 cactctgctg taatacactc tgaaaatcaa aacgtcataa atgaatttgc aaggcgagtc
    11521 cgcacaggaa gacttatcgt aaattcacca tcatcacagg gagcaatagg agatatatac
    11581 aatacaaaca cgccatcact tacattaggc tgtggttcta tgggaagaaa ctcaacgaca
    11641 gacaatgtaa gcgtcaagaa ccttttgaat attaagcgtg tcgtgataag gaaggataga
    11701 atgaaatggt tcaagattcc accgaagatt tactttgaaa gcgggtcact ccagtacctg
    11761 tgcaaagtca aaagaaaaaa agcgtttatc gtcacagatc cattcatggt taagcttggc
    11821 ttcgtagaca aagtgacata tcaattagac aaagcaaaca tcgaatacga aatattctca
    11881 gaagtagagc cagatccatc tgttgacaca gtcatgaacg gcgtaaaaat aatgaattcg
    11941 tacaatcctg acttaataat cgctgtaggc gatggctctg caatagacgc agcaaaggga
    12001 atgtggcttt tctacgaata tcctgataca gagtttgaaa cattgaggct taaatttgca
    12061 gacatcagaa aaagggcatt taagttccca gaacttggca aaaaagcgct attcatcgca
    12121 ataccgacaa caagcggcac aggctcagaa gtgacagcat ttgccgtaat aaccgacaaa
    12181 aagagaaaca tcaagtatcc actggcagac tacgaactta cacctgacat agccataata
    12241 gatcctgacc ttacaaagac tgtaccgcca tctgtaacag cagacacagg catggatgtg
    12301 ctgacacacg ccatagaagc atacgtatca gtaatggcat cagactacac agatgcactg
    12361 gcggaaaagg ctataaagat cgtatttgaa tacctgccaa gggcttataa aaacggcaat
    12421 gatgaagaag cccgcgaaaa gatgcacaat gcttcctgca tggctggtat ggcattcaca
    12481 aatgcattct taggaataaa ccacagcatg gcacacatac tgggcggaaa gttccacata
    12541 ccacacggaa gagcaaatgc aatacttctg ccgtatgtaa taaggtacaa tgcagaaaaa
    12601 cctacaaagt ttgtggcatt cccacaatac gaatatccaa aagcagcaga aagatatgcg
    12661 gaaatcgcca aattcttagg actgcctgct tcaactgttg aagaaggcgt agaaagctta
    12721 atagaagcta taaagaacct catgaaagag cttaacattc cgcttacact taaagacgcc
    12781 ggcatcaaca aagaacagtt tgaaaaagaa atagaggaaa tgtcagacat cgccttcaac
    12841 gatcagtgca cagggacaaa cccgagaatg cctctcacaa aagaaattgc agagatctac
    12901 agaaaagcat acggtgcata gtaaaggagg cgatattaat gtcactgatt ggaactgaag
    12961 taaaaccatt tactgctgag gcttatcaca acggaaaatt tataacggta acggagcagg
    13021 attttaaggg caaatggagc atagtagttt tctatcccgc agattttact tttgtttgcc
    13081 cgaccgagct tgaagacctt cagaataatt atgaaaaatt taaagcatta ggagcagaag
    13141 tttattctgt ttccaccgat acccattata ctcacaaagc atggcatgac acatccgaag
    13201 ctataaagaa aattacttat gtaatgattg gcgatccgtc ccatactctt tccagaaact
    13261 ttgatgttct caatgaagca acaggattgg cagacagagg tacctttatt atagaccctg
    13321 acggtgttat tcaatatgtt gaagtatcag ctgaaaatgt cggacgtaat gccgaggctc
    13381 ttattgataa aataaaggct gcacaatacg tcagaaatca tcccggtgaa gtttgcccgg
    13441 caaaatggaa agaaggcctg ctaatagtag tgaaaaaatc agggataatg ccgctctaac
    13501 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat
    13561 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt
    13621 gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc
    13681 tttaatttgc aagctgcggc cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    13741 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    13801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    13861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    13921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    13981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    14041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    14101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    14161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    14221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    14281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    14341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    14401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    14461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    14521 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    14581 cgttaaggga ttttggtcat gaaaaaatcc gc
//
