LOCUS       V003246                10397 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003246
VERSION     V003246
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Lanahan AA, Murphy SJL., Maloney MI,
            Zheng T, Papanek B, Guss AM, Lynd LR.
  TITLE     The ethanol pathway from Thermoanaerobacterium saccharolyticum
            improves ethanol production in Clostridium thermocellum
  JOURNAL   Metab. Eng. 42, 175-184 (2017)
   PUBMED   28663138
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   Hon S, Olson DG, Holwerda EK, Lanahan AA, Murphy SJL., Maloney MI,
            Zheng T, Papanek BA, Guss AM, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2017) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 10397)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10397)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            42, 175-184 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-MAR-2017) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..92
                     /label="cat promoter"
                     /note="cat promoter"
                     /regulatory_class="promoter"
     CDS             93..740
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(824..1681)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1682..1773)
                     /label="AmpR promoter"
     rep_origin      2033..2578
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             3049..4050
                     /label="repB"
                     /note="RepB replication protein"
     regulatory      4099..4307
                     /label="Clo1313_2638 promoter"
                     /note="Clo1313_2638 promoter"
                     /regulatory_class="promoter"
     CDS             4308..5507
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="NADPH-dependent alcohol dehydrogenase"
                     /protein_id="ASK86096.1"
                     /translation="MWETKVNPSKIFELRCKNTTYFGVGSIHKIKDILENLKINGINNV
                     IFITGKGSYKTSGAWDVVRPVLEELDLKYSLYDKVGPNPTVDMIDEAAKIGRESGAKAV
                     IGIGGGSPIDTAKSVAVLLKYTDKNARELYKQKFIPDDAVPIIAINLTHGTGTEVDRFA
                     VATIPEKNYKPAIAYDCLYPMFAIDDPSLMTKLDKKQTIAVTVDALNHITEAATTLVAS
                     PYSILTAKETVRLIVRYLPAAVNDPLNIVARYYLLYASALAGISFDNGLLHLTHALEHP
                     LSAVKPEIAHGLGLGAILPAVIKAIYPATAEVLADVYSPIVPGLKGLPVEAEYVAEKVQ
                     EWLFSVGCIQKLSDFGFTKDDIPNLVKLAKTTPSLDGLLSIAPVEATESVIEKIYLKSL
                     "
     gene            4308..5507
                     /gene="adhA"
                     /label="adhA"
     CDS             5525..6379
                     /codon_start=1
                     /gene="nfnA"
                     /product="NfnA"
                     /label="nfnA"
                     /note="NADH-dependent reduced ferredoxin:NADP+
                     oxidoreductase"
                     /protein_id="ASK86098.1"
                     /translation="MNEILEKKQLNPTVKMMVINAPLMAKKAKPGQFVIVRVDEKGERI
                     PLTIADYDRNKGTITIIFQEVGMSTKKLGTLNVGDRLHDFVGPLGKPVEFSKDTKRVLA
                     IGGGVGVAPLYPKVKMLNEMKVPVDSIIGGRSAEYVILEDEMKKVSENLYITTDDGTKG
                     RKGFVTDVLKELIEKDNKYDEVIAIGPLIMMKMVCNITKEYNIPTMVSMNPIMIDGTGM
                     CGGCRVTVGGETKFACVDGPAFDGLKVDFDEAMRRQNMYKDMERKVLENYEHECKLGGI
                     LNG"
     gene            5525..6379
                     /gene="nfnA"
                     /label="nfnA"
     CDS             6372..7763
                     /codon_start=1
                     /gene="nfnB"
                     /product="NfnB"
                     /label="nfnB"
                     /note="NADH-dependent reduced ferredoxin:NADP+
                     oxidoreductase"
                     /protein_id="ASK86099.1"
                     /translation="MANMSLKKVPMPEQEPDQRNKNFKEVALGYEENMAVEEAERCIQC
                     KNQPCVEGCPVHVKIPEFIKLIANRDFEGAYQKIKETNNLPAICGRVCPQESQCESVCT
                     RGKKGEPVAIGRLERFTADWHMKNNEDKIEKPETNGRKVAVIGSGPAGLSCAGDLAKMG
                     YDTTIFEAFHTPGGVLMYGIPEFRLPKEIVQKEIDSLKKLGVKIETNMVIGKILTIDDL
                     FDMGYEAVFIGTGAGLPKFMNIPGENLNGVYSANEFLTRINLMKAYDFPNSPTPVKVGK
                     KVAVVGGGNVAMDAARSAKRMGAEEVYIVYRRSEEEMPARLEEIHHAKEEGIIFKLLTN
                     PVRIIGDESGSVKGIECVNMVLGDVDESGRRRPVEEKGSEHVIDVDTVIIAIGQSPNPL
                     ITSTTEGLEKQRWGGIIVNEETLETSRRGVFAGGDAVTGAATVILAMGAGKKAAASIHK
                     YLSEK"
     gene            6372..7763
                     /gene="nfnB"
                     /label="nfnB"
     CDS             7781..10360
                     /codon_start=1
                     /gene="adhE"
                     /product="AdhE"
                     /label="adhE"
                     /note="bifunctional alcohol and aldehyde dehydrogenase"
                     /protein_id="ASK86097.1"
                     /translation="MATTKTELDVQKQIDLLVSRAQEAQKKFMSYTQEQIDAIVKAMAL
                     AGVDKHVELAKMAYEETKMGVYEDKITKNLFATEYVYHDIKNEKTVGIINENIEENYME
                     VAEPIGVIAGVTPVTNPTSTTMFKCLISIKTRNPIIFSFHPKAIKCSIAAAKVMYEAAL
                     KAGAPEGCIGWIETPSIEATQLLMTHPGVSLILATGGAGMVKAAYSSGKPALGVGPGNV
                     PCYIEKSANIKRAVSDLILSKTFDNGVICASEQAVIIDEEIADEVKKLMKEYGCYFLNK
                     DEIKKLEKFAIDEQSCAMSPAVVGQPAAKIAEMAGFKVPEGTKILVAEYEGVGPKYPLS
                     REKLSPILACYTVKDYNEGIKKCEEMTEFGGLGHSAVIHSENQNVINEFARRVRTGRLI
                     VNSPSSQGAIGDIYNTNTPSLTLGCGSMGRNSTTDNVSVKNLLNIKRVVIRKDRMKWFK
                     IPPKIYFESGSLQYLCKVKRKKAFIVTDPFMVKLGFVDKVTYQLDKANIEYEIFSEVEP
                     DPSVDTVMNGVKIMNSYNPDLIIAVGDGSAIDAAKGMWLFYEYPDTEFETLRLKFADIR
                     KRAFKFPELGKKALFIAIPTTSGTGSEVTAFAVITDKKRNIKYPLADYELTPDIAIIDP
                     DLTKTVPPSVTADTGMDVLTHAIEAYVSVMASDYTDALAEKAIKIVFEYLPRAYKNGND
                     EEAREKMHNASCMAGMAFTNAFLGINHSMAHILGGKFHIPHGRANAILLPYVIRYNAEK
                     PTKFVAFPQYEYPKAAERYAEIAKFLGLPASTVEEGVESLIEAIKNLMKELNIPLTLKD
                     AGINKEQFEKEIEEMSDIAFNDQCTGTNPRMPLTKEIAEIYRKAYGA"
     gene            7781..10360
                     /gene="adhE"
                     /label="adhE"
ORIGIN
        1 ttgactttta aaaaaggatt gattctaatg aagaaagcag acaagtaagc ctcctaaatt
       61 cactttagat aaaaatttag gaggcatatc aaatgaactt taataaaatt gatttagaca
      121 attggaagag aaaagagata tttaatcatt atttgaacca acaaacgact tttagtataa
      181 ccacagaaat tgatattagt gttttatacc gaaacataaa acaagaagga tataaatttt
      241 accctgcatt tattttctta gtgacaaggg tgataaactc aaatacagct tttagaactg
      301 gttacaatag cgacggagag ttaggttatt gggataagtt agagccactt tatacaattt
      361 ttgatggtgt atctaaaaca ttctctggta tttggactcc tgtaaagaat gacttcaaag
      421 agttttatga tttatacctt tctgatgtag agaaatataa tggttcgggg aaattgtttc
      481 ccaaaacacc tatacctgaa aatgcttttt ctctttctat tattccatgg acttcattta
      541 ctgggtttaa cttaaatatc aataataata gtaattacct tctacccatt attacagcag
      601 gaaaattcat taataaaggt aattcaatat atttaccgct atctttacag gtacatcatt
      661 ctgtttgtga tggttatcat gcaggattgt ttatgaactc tattcaggaa ttgtcagata
      721 ggcctaatga ctggctttta taatatgaga taatgccgac tgtacttttt acagtcggtt
      781 ttctaatgtc actagggctc gcctttggga agtttgaagg ttaccaatgc ttaatcagtg
      841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1741 acatatttga atgtatttag aaaaataaac aaaagagttt gaggaaaact taagaaaacg
     1801 ggaaactatt tcctgttgag aatgtgaaat taaatgataa aaagtgatat aatagggtca
     1861 ggcgtacaga agcctgacct ttttgcaaaa ttgttttatt ggcgcggcgt atatatacag
     1921 catatataca atgtaaattt atacaatcaa attgttgata caatcgaatt atgcggctaa
     1981 aaatttgctc atgagcctag aaatatttta tctgattaat aagatgatct tcttgagatc
     2041 gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt
     2101 tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc
     2161 agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact
     2221 cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg
     2281 gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc
     2341 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     2401 caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     2461 cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2521 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa
     2581 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa
     2641 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag
     2701 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca
     2761 gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt
     2821 aagccagtat acaaatgagt gctttttttg cgttttgagc gtagcgaaaa acgagttctt
     2881 tctattcttg atacatatag aaataacgtc atttttattt tagttgctga aaggtgcgtt
     2941 gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc ttaaaattgg ttgcacagaa
     3001 aaaccccatc tgttaaagtt ataagtgacc aaacaaataa ctaaatagat gggggtttct
     3061 tttaatatta tgtgtcctaa tagtagcatt tattcagatg aaaaatcaag ggttttagtg
     3121 gacaagacaa aaagtggaaa agtgagacca tggagagaaa agaaaatcgc taatgttgat
     3181 tactttgaac ttctgcatat tcttgaattt aaaaaggctg aaagagtaaa agattgtgct
     3241 gaaatattag agtataaaca aaatcgtgaa acaggcgaaa gaaagttgta tcgagtgtgg
     3301 ttttgtaaat ccaggctttg tccaatgtgc aactggagga gagcaatgaa acatggcatt
     3361 cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc caacagttcg ttggttgttt
     3421 ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat taaataagag tttgtcagat
     3481 atggctcaag gatttcgccg aatgatgcaa tataaaaaaa ttaataaaaa tcttgttggt
     3541 tttatgcgtg caacggaagt gacaataaat aataaagata attcttataa tcagcacatg
     3601 catgtattgg tatgtgtgga accaacttat tttaagaata cagaaaacta cgtgaatcaa
     3661 aaacaatgga ttcaattttg gaaaaaggca atgaaattag actatgatcc aaatgtaaaa
     3721 gttcaaatga ttcgaccgaa aaataaatat aaatcggata tacaatcggc aattgacgaa
     3781 actgcaaaat atcctgtaaa ggatacggat tttatgaccg atgatgaaga aaagaatttg
     3841 aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa ggttaatctc ctatggtggt
     3901 ttgttaaaag aaatacataa aaaattaaac cttgatgaca cagaagaagg cgatttgatt
     3961 catacagatg atgacgaaaa agccgatgaa gatggatttt ctattattgc aatgtggaat
     4021 tgggaacgga aaaattattt tattaaagag tagttcaacc cggggatcct ctagagtcga
     4081 cctgcaggca tgcaagctga taaacaaagg acggttcagg gcttctgctc atcctactct
     4141 gcattgtaaa aaggtaggat gaatttttat ttttaatctt attgaaaaaa atttttgaaa
     4201 atcggtttta ttaaaaaaaa gtgggtatat ttataatagt caattgattg gttaaaaaaa
     4261 tttaaataag caaacagaat aataacaaaa gtaaggagga atttgttatg tgggaaacaa
     4321 aagtaaatcc tagcaaaatt tttgaacttc gctgtaaaaa tacgacttat tttggtgttg
     4381 gcagtataca taagataaag gatatattag aaaatctaaa aattaatggc attaataatg
     4441 ttatttttat aacaggcaaa ggttcgtata agactagcgg agcttgggat gttgtaaggc
     4501 ctgtattgga agagcttgat ttaaaatatt cactttacga taaagttggt ccaaatccta
     4561 ccgttgatat gatagacgaa gcggcaaaaa ttggacgaga atctggagcg aaagccgtaa
     4621 ttggaattgg cggtggtagc cctatagata ctgcaaaaag tgtagctgtg ctattaaagt
     4681 atactgataa aaatgcacga gagctttata agcaaaaatt tattcctgat gatgctgttc
     4741 ctataatagc tataaatctt acacatggta caggaacaga ggtagataga tttgcagtgg
     4801 ctactatacc tgagaaaaat tataaaccag ctatagctta tgattgctta tatcctatgt
     4861 ttgctataga tgacccttcg cttatgacaa agcttgataa aaaacagact atagccgtta
     4921 ctgttgatgc tttaaatcac attacagaag ctgctacgac acttgttgca tctccatata
     4981 gtattcttac ggctaaggaa acagtaagat tgattgttcg atatcttccc gctgctgtaa
     5041 atgatccttt aaatatagtg gcaagatatt accttctgta tgcttctgca ttagctggca
     5101 tatcttttga caatggtctt ttgcatctta ctcatgcttt agagcatcct ttaagtgctg
     5161 taaaacctga aatagctcat ggtttagggc ttggtgcaat attgccagca gttataaaag
     5221 ctatttatcc agctacagca gaagtattgg ctgatgtata tagtcctata gttcctggtt
     5281 taaaaggact gcctgttgag gcggagtatg tagcagaaaa agttcaagaa tggcttttta
     5341 gtgtggggtg cattcaaaag ttatctgatt ttgggtttac gaaagatgat attcctaatc
     5401 ttgtaaaact tgctaaaaca actccttccc ttgatgggtt gttatctatt gcacctgttg
     5461 aagctactga atctgtcatt gaaaagattt atttaaaatc tctttaataa aggaggttat
     5521 aaaaatgaac gagattttag agaagaagca attgaatccc actgtcaaaa tgatggttat
     5581 aaacgctcca ctaatggcga aaaaggcgaa acctggacag tttgtcatag taagagtaga
     5641 tgaaaagggt gaacgcattc ctttgacaat agcggattac gacagaaaca aaggcacgat
     5701 tactataata tttcaagaag tcggcatgag caccaagaag ttaggtacgt taaacgtagg
     5761 cgatagattg catgacttcg taggaccttt aggaaaacct gttgaatttt caaaagatac
     5821 gaagagagtt ttagcaatcg gcggaggtgt tggagttgcg ccgctttatc caaaagtaaa
     5881 aatgctaaat gagatgaaag taccagtgga tagtataata ggcggcagaa gtgcagagta
     5941 cgtcatatta gaagatgaaa tgaaaaaagt cagtgaaaac ctttatataa cgacggatga
     6001 cggcacaaaa ggccgtaaag gctttgtaac agatgtatta aaagaattga tagaaaaaga
     6061 caacaaatac gatgaagtaa ttgcaattgg gccactgata atgatgaaga tggtatgcaa
     6121 tataacaaaa gaatacaaca taccaactat ggtcagcatg aacccgataa tgatagacgg
     6181 aactggaatg tgcggaggat gcagagtcac agttggagga gagacaaaat ttgcatgtgt
     6241 cgatggtcca gcatttgacg ggcttaaagt agactttgat gaagcaatga gaaggcagaa
     6301 tatgtataaa gatatggaga ggaaagtatt agaaaattat gagcacgaat gcaagttagg
     6361 gggcatctta aatggctaac atgtcgttaa aaaaagtacc aatgcctgaa caggaaccag
     6421 atcagcggaa caaaaatttc aaagaagttg cattagggta tgaagaaaac atggctgttg
     6481 aagaagccga aagatgtatt caatgtaaga atcagccatg tgttgaaggt tgccctgttc
     6541 acgtaaaaat acctgagttc atcaagctga tagcaaacag ggactttgaa ggagcatatc
     6601 aaaagataaa agaaacgaat aatctacctg caatatgtgg aagagtatgt ccacaggaaa
     6661 gccagtgtga atcagtatgc acaagaggca aaaaaggaga acctgtagct ataggcagac
     6721 tggagagatt tacagcagat tggcatatga aaaataatga agataaaata gaaaaaccag
     6781 agacgaatgg cagaaaagtt gctgttatag gatctgggcc tgcaggacta tcgtgtgcag
     6841 gtgatcttgc aaaaatggga tatgacacaa ccatctttga agcatttcat acaccaggcg
     6901 gtgttttaat gtacggtatc ccagagttta gacttccaaa agaaatagtg caaaaagaaa
     6961 ttgattcgtt gaaaaaattg ggagtaaaaa tagaaaccaa catggtcata ggcaagatac
     7021 ttacaatcga tgatttattc gacatggggt atgaagcagt gttcataggg acaggcgcag
     7081 gattgcctaa atttatgaat atacctggag aaaacttaaa tggagtttac tcagcaaatg
     7141 aatttttgac gaggattaat ttgatgaaag cttatgattt ccctaacagt cccactcctg
     7201 taaaagtagg caagaaagtt gctgttgttg gcggtggaaa cgtggcgatg gatgcagcaa
     7261 gatccgcaaa gcgcatggga gcagaagaag tgtacatcgt atatagaagg tcagaggaag
     7321 aaatgcctgc aaggcttgag gaaatacacc acgcaaaaga agaaggaatc atatttaagt
     7381 tattgacaaa ccctgtaaga atcattggtg atgaaagtgg cagtgtaaaa ggaatcgaat
     7441 gtgttaacat ggttttaggc gatgtagacg aatcaggtag aagaagacct gtagaagaaa
     7501 aaggctcaga gcatgtaata gatgtagata cagttattat agcaatagga caaagtccaa
     7561 atccgcttat aacatccaca acagaaggtt tagaaaagca aagatggggt ggaataatag
     7621 tcaacgaaga aacattggag acaagccgca gaggagtttt tgcgggcggt gatgctgtga
     7681 caggtgcagc tacagtaata cttgcaatgg gagctggtaa aaaagctgca gcttctatac
     7741 acaaatattt aagcgaaaag tgataaagga ggtcgacgtc atggcaacga caaaaacgga
     7801 attagacgtt cagaagcaga tagatctact tgtgtcaaga gcacaagagg ctcagaaaaa
     7861 attcatgtct tacacgcaag agcaaatcga cgcaatagtt aaggcaatgg ctttagcagg
     7921 cgttgacaaa cacgtagagc tggcaaagat ggcgtacgaa gagacaaaaa tgggtgtata
     7981 cgaagataag ataacaaaaa atctcttcgc aacagagtac gtgtaccacg acataaaaaa
     8041 tgaaaagact gtaggaatca taaacgagaa catagaagaa aactacatgg aagtggcaga
     8101 accgataggc gtaattgccg gtgtcacacc tgtcacaaac ccaacatcta ccacgatgtt
     8161 taaatgctta atatccataa agacgcgaaa tcctataata ttcagcttcc atccaaaggc
     8221 aataaagtgc agcatcgcag cagccaaagt gatgtatgaa gctgcactaa aggcaggcgc
     8281 acctgaagga tgcataggat ggatagaaac gccatcaatt gaggccacac agcttctcat
     8341 gacacatcca ggcgtatcgc tgatccttgc aacgggcggt gcaggaatgg taaaagcggc
     8401 atacagctca ggaaaaccgg cattaggcgt aggtcctggc aatgtgccat gctacatcga
     8461 aaaatcagca aacataaaga gggctgtatc ggatctcata ctaagcaaga catttgacaa
     8521 tggagtaata tgcgcatcag agcaggccgt aataatagac gaggaaatag cagatgaagt
     8581 caaaaagctt atgaaagaat acggctgcta cttcttaaac aaagatgaaa taaagaagct
     8641 tgagaaattt gcaattgatg agcaaagctg cgccatgagc cctgcagtgg taggtcagcc
     8701 agcggcgaag attgctgaaa tggcaggctt caaagtcccc gaaggcacaa agatattagt
     8761 ggcagagtac gaaggagtag gtccaaaata tcctctatca agggagaaac taagcccgat
     8821 tcttgcttgc tacaccgtca aagactacaa tgaaggaatc aaaaagtgcg aggaaatgac
     8881 tgaattcgga ggtttaggcc actctgctgt aatacactct gaaaatcaaa acgtcataaa
     8941 tgaatttgca aggcgagtcc gcacaggaag acttatcgta aattcaccat catcacaggg
     9001 agcaatagga gatatataca atacaaacac gccatcactt acattaggct gtggttctat
     9061 gggaagaaac tcaacgacag acaatgtaag cgtcaagaac cttttgaata ttaagcgtgt
     9121 cgtgataagg aaggatagaa tgaaatggtt caagattcca ccgaagattt actttgaaag
     9181 cgggtcactc cagtacctgt gcaaagtcaa aagaaaaaaa gcgtttatcg tcacagatcc
     9241 attcatggtt aagcttggct tcgtagacaa agtgacatat caattagaca aagcaaacat
     9301 cgaatacgaa atattctcag aagtagagcc agatccatct gttgacacag tcatgaacgg
     9361 cgtaaaaata atgaattcgt acaatcctga cttaataatc gctgtaggcg atggctctgc
     9421 aatagacgca gcaaagggaa tgtggctttt ctacgaatat cctgatacag agtttgaaac
     9481 attgaggctt aaatttgcag acatcagaaa aagggcattt aagttcccag aacttggcaa
     9541 aaaagcgcta ttcatcgcaa taccgacaac aagcggcaca ggctcagaag tgacagcatt
     9601 tgccgtaata accgacaaaa agagaaacat caagtatcca ctggcagact acgaacttac
     9661 acctgacata gccataatag atcctgacct tacaaagact gtaccgccat ctgtaacagc
     9721 agacacaggc atggatgtgc tgacacacgc catagaagca tacgtatcag taatggcatc
     9781 agactacaca gatgcactgg cggaaaaggc tataaagatc gtatttgaat acctgccaag
     9841 ggcttataaa aacggcaatg atgaagaagc ccgcgaaaag atgcacaatg cttcctgcat
     9901 ggctggtatg gcattcacaa atgcattctt aggaataaac cacagcatgg cacacatact
     9961 gggcggaaag ttccacatac cacacggaag agcaaatgca atacttctgc cgtatgtaat
    10021 aaggtacaat gcagaaaaac ctacaaagtt tgtggcattc ccacaatacg aatatccaaa
    10081 agcagcagaa agatatgcgg aaatcgccaa attcttagga ctgcctgctt caactgttga
    10141 agaaggcgta gaaagcttaa tagaagctat aaagaacctc atgaaagagc ttaacattcc
    10201 gcttacactt aaagacgccg gcatcaacaa agaacagttt gaaaaagaaa tagaggaaat
    10261 gtcagacatc gccttcaacg atcagtgcac agggacaaac ccgagaatgc ctctcacaaa
    10321 agaaattgca gagatctaca gaaaagcata cggtgcatag ttaatagaaa taattttgtt
    10381 taactttaca aacggga
//
